Immunology

A novel bioinformatics pipeline for the identification of immune inhibitory receptors as potential therapeutic targets

1. Akashdip Singh
2. Alberto Miranda Bedate
3. Helen J von Richthofen
4. Saskia V Vijver
5. Michiel van der Vlist
6. Raphael Kuhn
7. Alexander Yermanos
8. Jürgen J Kuball
9. Can Kesmir
10. M Ines Pascoal Ramos
11. Linde Meyaard

• Curated by eLife

  eLife assessment

  The authors presented a valuable bioinformatics pipeline for screening and identifying inhibitory receptors for potential drug targets. They provided solid evidence showing a sequential reduction in the search space through various screening tools and algorithms and demonstrated that this pipeline can be used to "rediscover" known targets. Further experimental validation on putative and unknown inhibitory receptors will strengthen the evidence reported in this work. This study will be of interest to bioinformaticians and computational biologists working on immune regulation, sequence screening, and target identification of immune checkpoint inhibitors.

Reviewed by eLife

Chemokine expression profile of an innate granuloma

1. Megan E Amason
2. Cole J Beatty
3. Carissa K Harvest
4. Daniel R Saban
5. Edward A Miao

• Curated by eLife

  eLife assessment

  This valuable study advances the understanding of granuloma formation by identifying a key chemokine receptors in containing infection by a specific species of bacteria. The evidence supporting this is solid, providing a spatial transcriptomic dataset spanning granuloma formation and resolution by a specific species of bacteria. The work should be of interest to microbiologists and immunologists.

Reviewed by eLife

Cell state and transcription factor modulation during extended ex vivo CD8 ⁺ T-cell expansion

1. Yuan Lui
2. Edward Jenkins
3. Emily Zhi Qing Ng
4. Mateusz Kotowski
5. Sydney J Mullin
6. Joseph Clarke
7. Simon J Davis
8. Ana Mafalda Santos
9. Sumana Sharma

Reviewed by PREreview

Resting natural killer cells promote the progress of colon cancer liver metastasis by elevating tumor-derived stem cell factor

1. Chenchen Mao
2. Yanyu Chen
3. Dong Xing
4. Teming Zhang
5. Yangxuan Lin
6. Cong Long
7. Jiaye Yu
8. Yunhui Luo
9. Tao Ming
10. Wangkai Xie
11. Zheng Han
12. Dianfeng Mei
13. Dan Xiang
14. Mingdong Lu
15. Xian Shen
16. Xiangyang Xue

• Curated by eLife

  eLife assessment

  This useful study draws on published single-cell and spatial transcriptomic data of colon cancer liver metastasis to clarify the pro- and anti-tumorigenic properties of NK cells. The authors discover increased GZMK+ resting NK cells in the tumor tissue and reduced abundance of KIR2DL4+ activated NK cells. However, the evidence is currently incomplete, as the models used to validate the hypothesis and claims are not adequate and lack the necessary controls.

Reviewed by eLife

Exposure to live saprophytic Leptospira before challenge with a pathogenic serovar prevents severe leptospirosis and promotes kidney homeostasis

1. Suman Kundu
2. Advait Shetty
3. Maria Gomes-Solecki

• Curated by eLife

  eLife assessment

  This important study contributes to our understanding on how prior exposure to a non-pathogenic Leptospira strain could prime the host to prevent severe leptospirosis following infection with a pathogenic strain. The work described is solid and broadly supports the claims, with minor weaknesses that could be addressed in future studies. The work will be of interest to scientists interested in host-pathogen interactions and leptospirosis.

Reviewed by eLife

Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross-stage recognition of glutamate-rich repeats

1. Axelle Amen
2. Randy Yoo
3. Amanda Fabra-García
4. Judith Bolscher
5. William JR Stone
6. Isabelle Bally
7. Sebastián Dergan-Dylon
8. Iga Kucharska
9. Roos M de Jong
10. Marloes de Bruijni
11. Teun Bousema
12. C Richter King
13. Randall S MacGill
14. Robert W Sauerwein
15. Jean-Philippe Julien
16. Pascal Poignard
17. Matthijs M Jore

• Curated by eLife

  eLife assessment

  This study reports important results and new insights into humoral immune responses to Plasmodium falciparum sexual stage proteins. The experiments are based on the use of target-agnostic memory B cell sorting and screening approaches as well as several state-of-the-art technologies. The authors present compelling evidence that one antibody, B1E11K, is cross-reactive with multiple proteins containing glutamate-rich repeats through homotypic interactions, a process similar to what has been observed for Plasmodium circumsporozoite protein repeat-directed antibodies.

Reviewed by eLife

Transposable elements may enhance antiviral resistance in HIV-1 elite controllers

1. Manvendra Singh
2. Sabrina M. Leddy
3. Luis Pedro Iñiguez
4. Matthew L. Bendall
5. Douglas F. Nixon
6. Cédric Feschotte

Reviewed by Review Commons

Tissue-resident memory CD8 T cell diversity is spatiotemporally imprinted

1. Miguel Reina-Campos
2. Alexander Monell
3. Amir Ferry
4. Vida Luna
5. Kitty P. Cheung
6. Giovanni Galletti
7. Nicole E. Scharping
8. Kennidy K. Takehara
9. Sara Quon
10. Peter P. Challita
11. Brigid Boland
12. Yun Hsuan Lin
13. William H. Wong
14. Cynthia S. Indralingam
15. Hayley Neadeau
16. Suzie Alarcón
17. Gene W. Yeo
18. John T. Chang
19. Maximilian Heeg
20. Ananda W. Goldrath

Reviewed by preLights

Adipocyte microRNA-802 promotes adipose tissue inflammation and insulin resistance by modulating macrophages in obesity

1. Yue Yang
2. Bin Huang
3. Yimeng Qin
4. Danwei Wang
5. Yinuo Jin
6. Linmin Su
7. Qingxin Wang
8. Yi Pan
9. Yanfeng Zhang
10. Yumeng Shen
11. Wenjun Hu
12. Zhengyu Cao
13. Liang Jin
14. Fangfang Zhang

• Curated by eLife

  eLife assessment

  This important study utilizes a comprehensive array of animal and cellular models, alongside various techniques, to elucidate the mechanism by which adipose tissue miR-802 contributes to inflammation and metabolic dysfunction in obesity. The data is solid, with clear, reproducible changes showing low variability among biological replicates and consistency across different models. However, some conclusions should be further substantiated with additional data to enhance the scope and strength of the manuscript.

Reviewed by eLife

Defining cell type-specific immune responses in a mouse model of allergic contact dermatitis by single-cell transcriptomics

1. Youxi Liu
2. Meimei Yin
3. Xiaoting Mao
4. Shuai Wu
5. Shuangping Wei
6. Shujun Heng
7. Yichun Yang
8. Jinwen Huang
9. Zhuolin Guo
10. Chuan Li
11. Chao Ji
12. Liu Hu
13. Wenjie Liu
14. Ling-juan Zhang

• Curated by eLife

  eLife assessment

  This important study uses single-cell RNA-seq to obtain a more granular understanding of cell subsets within allergic contact dermatitis in a model system with DNFB. The convincing data revela unique subpopulations of dermal fibroblasts as key responders to interferon gamma and likely as mediators of dermatitis. This study has many novel aspects and provides a unique resource as well.

Reviewed by eLife