Immunology

Human giant GTPase GVIN1 forms an antimicrobial coatomer around the intracellular bacterial pathogen Burkholderia thailandensis

1. Weilun Guo
2. Shruti S Apte
3. Mary S Dickinson
4. So Young Kim
5. Miriam Kutsch
6. Jörn Coers

• Curated by eLife

  eLife Assessment

  This fundamental study identifies GVIN as a previously unrecognized interferon-induced antimicrobial GTPase and establishes its role in cell-autonomous immunity against intracellular Burkholderia. The compelling data demonstrate that GVIN assembles into coat-like structures on the bacterial surface and restricts BimA-dependent actin-based motility through a mechanism independent of GBP1. These findings expand our understanding of interferon-induced host defense mechanisms and reveal a distinct pathway by which human cells restrict the intracellular spread of bacterial pathogens.

Reviewed by eLife

Temperature-based MHC class-I multimer peptide exchange for human HLA-A, B and C

1. Cilia R Pothast
2. Ian Derksen
3. Anneloes van der Plas - van Duijn
4. Angela el Hebieshy
5. Wesley Huisman
6. Kees LMC Franken
7. Jacques Neefjes
8. Jolien J Luimstra
9. Marieke Griffioen
10. Michel Kester
11. Maarten H Vermeer
12. Marcus Östholm
13. Sine R Hadrup
14. Mirjam HM Heemskerk
15. Ferenc A Scheeren

• Curated by eLife

  eLife Assessment

  This is a very helpful study that presents an easy to use and reliable alternative to conventional and UV-based tetramers for the identification of antigen-specific CD8 T cells. The authors demonstrate that tetramers for a number of HLA alleles can be subjected to specific temperatures that facilitate peptide exchange whilst maintaining structural integrity, thereby generating detection reagents. They provide convincing evidence for this method, and the approach is likely to provide a useful alternative to generating reagents for examining T cell specificities.

Reviewed by eLife

Macrophages drive inguinal fat pad and lymph node remodelling in response to peripheral inflammation

1. Robin Bartolini
2. Deepika Sharma
3. Gillian J Wilson
4. Gillian Dunphy
5. Jonathan Cavanagh
6. Jennifer A Barrie
7. Heba A Halawa
8. John Cole
9. Stefan Weidt
10. Kirstyn Gardner-Stephen
11. Gerard J Graham

• Curated by eLife

  eLife Assessment

  This study presents important findings linking peripheral inflammation to the remodeling of perinodal adipose tissue and draining lymph nodes, suggesting a mechanism by which local tissue inflammation can reshape LN structure and metabolism. The evidence is solid and supported by observations, future studies will address any outstanding questions.

Reviewed by eLife

Gelsolin Counteracts ER Stress-Driven Inflammatory Circuits in Psoriasis-like Dermatitis

1. Daisuke Ori
2. Haruna Okude
3. Riko Konishi
4. Motoya Murase
5. Shuya Hiroki
6. Saki Takahara
7. Towa Tanaka
8. Rina Toyodome
9. Norisuke Kano
10. Takumi Kawasaki
11. Ken J Ishii
12. Kouji Kobiyama
13. Hideyuki Nakashima
14. Kinichi Nakashima
15. Miwa Sasai
16. Masahiro Yamamoto
17. Yutaro Kumagai
18. Akio Tsuru
19. Kenji Kohno
20. Taro Kawai

• Curated by eLife

  eLife Assessment

  This paper provides a valuable observation that imiquimod, a compound often used to induce a psoriasis-like skin inflammation in mice, has a TLR7-independent effect acting through the unfolded protein response and binding to Gelsolin. These solid findings expand our understanding of imiquimod-mediated inflammation and are of interest to the field of skin immunology.

Reviewed by eLife

Heterogeneity and ontogeny of mouse thymic macrophages reveal a requirement for Csf1r-expressing myeloid cells during early T cell development

1. Helen Wang
2. Vinothkumar Rajan
3. Anthony Wong
4. Slava Epelman
5. Juan Carlos Zúñiga-Pflücker

• Curated by eLife

  eLife Assessment

  This study characterises the heterogeneity and developmental origins of macrophages in the thymus and demonstrates a role for Csf1r-expressing myeloid cells in the first step of T cell development. The state-of-the-art experiments provide convincing evidence, and the assessors concerns were addressed in the revised submission. These results provide an important advance that furthers our understanding of thymus biology, especially in view of the contribution of heterogenous thymic macrophage subpopulations.

Reviewed by eLife

A Germinal Center Checkpoint of AIRE in B Cells Limits Antibody Diversification

1. Jordan Z Zhou
2. Bo Pei
3. Guang Wen Sun
4. Michael D Pawlitz
5. Wei Zhang
6. Xinyang Li
7. Fayi Yao
8. Kati C Hokynar
9. Chun-Xiao Li
10. Madusha LW Perera
11. Shanqiao Wei
12. Simin Zheng
13. Lisa A Polin
14. Janet M Poulik
15. Annamari Ranki
16. Kai Krohn
17. Charlotte Cunningham-Rundles
18. Andrea Cerutti
19. Naibo Yang
20. Ashok S Bhagwat
21. Kefei Yu
22. Pärt Peterson
23. Kai Kisand
24. Bao Q Vuong
25. Bihui Huang
26. Kang Chen

• Curated by eLife

  eLife Assessment

  AIRE has been well known to contribute to immune self-tolerance in the thymus by expressing auto-antigens. In this manuscript, the authors describe unexpected findings about the interaction of AIRE with AID in B cells, and its function in the immune system, thereby contributing to a fundamental understanding of the broader functions of AIRE. The strength of this manuscript is that, by employing biochemical and genetic experiments, the authors convincingly show interaction between AIRE and AID and subsequent AIRE's function in the GC responses.

Reviewed by eLife

An IL-21R STAT hypomorph circumvents functional redundancy in germinal centre responses

1. Christoph Jandl
2. Joanna Warren
3. Samantha Owens
4. Marcel Batten
5. Howard Wang
6. Cecile King

• Curated by eLife

  eLife Assessment

  These results add to a growing body of literature demonstrating a role for IL-21 in T follicular helper cell expansion and differentiation, as well as in germinal center formation and maintenance. The authors use an IL21R ENU-induced mutant, together with complementary in vitro and in vivo experiments, to investigate the roles of STAT1 and STAT3 signaling downstream of IL-21R. The experimental approach is valuable, but the main conclusion is incompletely supported by the evidence, as the mutant exhibits a broad reduction in STAT signaling rather than a selective impairment of STAT1 signaling.

Reviewed by eLife

UBA1 Mutations Drive RIPK1-Mediated Cell Death and Monocyte Dysfunction in VEXAS Syndrome

1. Paul Breillat
2. Samuel J. Magaziner
3. Stéphane Camus
4. Léa Dionet
5. Benjamin de Valence
6. Pierre Sohier
7. Amine Majdi
8. Quentin Delcros
9. Federica Pallotti
10. Nadia Rivet
11. Kevin Chevalier
12. Margot Poux
13. Pierre-Louis Tharaux
14. Olivia Lenoir
15. Abdelrahim Zoued
16. Olivier Kosmider
17. David B. Beck
18. Benjamin Terrier

Reviewed by PREreview

Role of mast cells in endomicrobial sepsis revisited: Mast cell-deficient mice show normal immunological protection

1. Thorsten B Feyerabend
2. Fabienne Schochter
3. Alpaslan Tasdogan
4. Maren Bechberger
5. Sébastien Boutin
6. Hans-Reimer Rodewald

• Curated by eLife

  eLife Assessment

  This study presents important evidence showing that the high susceptibility to sepsis of Kit-mutant mice is not due to mast cell deficiency. The revised manuscript, with solid data, focuses on refuting the notion that mast cells play a major role in sepsis. This paper would be of interest to researchers in mast cell biology and mucosal immunology.

Reviewed by eLife

WASp activity in macrophages prevents mechano-induced inflammation by protecting the nuclear envelope

1. Roberto Amadio
2. Giulia Bracchetti
3. Zahraa Alraies
4. Giulia Maria Piperno
5. Lucia Lopez Rodriguez
6. Mathieu Maurin
7. Martina Conti
8. Laura Andolfi
9. Maria Carmina Castiello
10. Francesca Ferrua
11. Anna Villa
12. Alessandro Aiuti
13. Ana-Maria Lennon-Dumenil
14. Federica Benvenuti

Reviewed by Review Commons