Immunology

Photoreceptor loss does not recruit neutrophils despite strong microglial activation

1. Derek Power
2. Justin Elstrott
3. Jesse Schallek

• Curated by eLife

  eLife Assessment

  The study by Power and colleagues is important, as elucidating the dynamic immune responses to photoreceptor damage in vivo potentiates future work in the field to better understand the disease process. The evidence supporting the authors' claims is compelling.

Reviewed by eLife

Pre-existing YFV-17D immunity mediates T cell cross-protection against DENV-2 infection

1. Prince Baffour Tonto
2. Sebastian Gallon
3. Reem Alatrash
4. Wei-Kung Wang
5. Bobby Brooke Herrera

Reviewed by Rapid Reviews Infectious Diseases, PREreview

Neutrophil KLF2 regulates inflammasome-dependent neonatal mortality from endotoxemia

1. Devashis Mukherjee
2. Sriram Satyavolu
3. Asha Thomas
4. Sarah Cioffi
5. Yuexin Li
6. E. Ricky Chan
7. Katherine Wen
8. Alex Y. Huang
9. Mukesh K. Jain
10. George R. Dubyak
11. Lalitha Nayak

Reviewed by preLights

A randomized controlled trial of the effectiveness of a community-based rabies vaccination strategy

1. Felix Lankester
2. Ahmed Lugelo
3. Joel Changalucha
4. Danni Anderson
5. Christian Tetteh Duamor
6. Anna Czupryna
7. Kennedy Lushasi
8. Elaine Ferguson
9. Emmanuel S. Swai
10. Hezron Nonga
11. Maganga Sambo
12. Sarah Cleaveland
13. Sally Wyke
14. Paul C. D. Johnson
15. Katie Hampson

Reviewed by PREreview

Temperature-based MHC class-I multimer peptide exchange for human HLA-A, B and C

1. Cilia R Pothast
2. Ian Derksen
3. Anneloes van der Plas - van Duijn
4. Angela el Hebieshy
5. Wesley Huisman
6. Kees LMC Franken
7. Jacques Neefjes
8. Jolien J Luimstra
9. Marieke Griffioen
10. Michel Kester
11. Maarten H Vermeer
12. Mirjam HM Heemskerk
13. Ferenc A Scheeren

• Curated by eLife

  eLife Assessment

  This study presents an alternative to conventional and UV-based tetramers, which are easy to use and reliable for the identification of antigen-specific CD8 T cells. The authors demonstrate that tetramers for HLA alleles A0301, A1101, B0702, and C0702 can be subjected to specific temperatures that facilitate peptide exchange, whilst maintaining structural integrity. Whilst the strength of the evidence is currently incomplete, further development and validation of this approach is likely to provide a useful alternative to generating reagents for examining T cell specificities.

Reviewed by eLife

Hypothermia protects against ventilator-induced lung injury by limiting IL-1β release and NETs formation

1. Nobuyuki Nosaka
2. Vanessa Borges
3. Daisy Martinon
4. Timothy R Crother
5. Moshe Arditi
6. Kenichi Shimada

• Curated by eLife

  eLife Assessment

  This study provides a comprehensive exploration of the role of hypothermia of mitigating IL1beta induction and NETosis in the context of lung injury induced by mechanical ventilation. The data are convincing, and the study is important for the field.

Reviewed by eLife

Somatic hypermutation patterns in immunoglobulin variable regions are established independently of the local transcriptional landscape

1. Ursula E Schoeberl
2. Johanna Fitz
3. Kimon Froussios
4. Renan Valieris
5. Iordanis Ourailidis
6. Marina Makharova
7. Bernd Bauer
8. Tobias Neumann
9. Eva-Maria Wiedemann
10. Monika Steininger
11. Adriana Cantoran Garcia
12. Marialaura Mastrovito
13. Hugo Mouquet
14. Israel Tojal Da Silva
15. Rushad Pavri

• Curated by eLife

  eLife Assessment

  This study clarifies that stalled RNA pol II is not sufficient for AID targeting, which is important to the field. The authors provide solid experimental evidence that RNA poll II stalling is not the driving mechanism for AID targeting, and even though the results are generally "negative", they are highly relevant to our current understanding of SHM. The authors propose premature transcription termination as a possible mechanism to determine V gene mutability, but the study does not experimentally address such possibilities. This paper makes investigators rethink the model with which AID finds single-strand DNA in the genome.

Reviewed by eLife

An immunomechanical checkpoint PYK2 governs monocyte-to-macrophage differentiation in pancreatic cancer

1. Wenyan Xie
2. Xin Yu
3. Qingxin Yang
4. Nengwen Ke
5. Ping Wang
6. Hao Kong
7. Xiangji Wu
8. Panpan Ma
9. Lang Chen
10. Jie Yang
11. Xiuqing Feng
12. Yuan Wang
13. Hubing Shi
14. Lu Chen
15. Yun-Hua Liu
16. Bi-Sen Ding
17. Qiang Wei
18. Hong Jiang

Reviewed by preLights

Early resident NK cell response to local HIV infection in lymphoid tissue

1. David Perea
2. Alba Gonzalez
3. Nerea Sanchez-Gaona
4. Felix Pumarola
5. Nuria Ortiz
6. Ines Llano
7. Juan Lorente
8. Vicenc Falco
9. Meritxell Genesca
10. Maria J Buzon

Reviewed by preLights

Single-cell profiling of the lung immune cells of diabetes-tuberculosis comorbidity reveals reduced type-II interferon and elevated Th17 responses

1. Shweta Chaudhary
2. Mothe Sravya
3. Falak Pahwa
4. Sureshkumar V
5. Prateek Singh
6. Shivam Chaturvedi
7. Debasisa Mohanty
8. Debasis Dash
9. Ranjan Kumar Nanda

• Curated by eLife

  eLife Assessment

  This study presents a valuable finding on the intersection between tuberculosis and diabetes and the impact on immune responses, notably T cell and myeloid cell responses. The single-cell data collected and analyzed are convincing and provide a rich dataset to develop a more detailed understanding of cellular responses during Mtb infection of diabetic mice. Some of the mechanistic claims are incomplete, as there are no experiments performed to clearly define a role for IL-16 or IL-17 in disease. Inclusion of analysis of human samples would have strengthened the conclusions in the paper for translational impact, as well as the inclusion of a DM group alone in addition to DM-TB vs TB in some of the experiments.

Reviewed by eLife