Immunology

Cell state and transcription factor modulation during extended ex vivo CD8 ⁺ T-cell expansion

1. Yuan Lui
2. Edward Jenkins
3. Emily Zhi Qing Ng
4. Mateusz Kotowski
5. Sydney J Mullin
6. Joseph Clarke
7. Simon J Davis
8. Ana Mafalda Santos
9. Sumana Sharma

Reviewed by PREreview

Resting natural killer cells promote the progress of colon cancer liver metastasis by elevating tumor-derived sSCF

1. Chenchen Mao
2. Yanyu Chen
3. Dong Xing
4. Teming Zhang
5. Yangxuan Lin
6. Cong Long
7. Jiaye Yu
8. Yunhui Luo
9. Tao Ming
10. Wangkai Xie
11. Zheng Han
12. Dianfeng Mei
13. Dan Xiang
14. Mingdong Lu
15. Xian Shen
16. Xiangyang Xue

• Curated by eLife

  eLife assessment

  This useful study draws on published single-cell and spatial transcriptomic data of colon cancer liver metastasis to clarify the pro- and anti-tumorigenic properties of NK cells. The authors discover increased GZMK+ resting NK cells in the tumor tissue and reduced abundance of KIR2DL4+ activated NK cells. However, the evidence is currently incomplete, as the models used to validate the hypothesis and claims are not adequate and lack the necessary controls.

Reviewed by eLife

Exposure to live saprophytic Leptospira before challenge with a pathogenic serovar prevents severe leptospirosis and promotes kidney homeostasis

1. Suman Kundu
2. Advait Shetty
3. Maria Gomes-Solecki

• Curated by eLife

  eLife assessment

  This important study contributes to our understanding on how prior exposure to a non-pathogenic Leptospira strain could prime the host to prevent severe leptospirosis following infection with a pathogenic strain. The work described is solid and broadly supports the claims, with minor weaknesses that could be addressed in future studies. The work will be of interest to scientists interested in host-pathogen interactions and leptospirosis.

Reviewed by eLife

T3SS translocon induces pyroptosis by direct interaction with NLRC4/NAIP inflammasome

1. Yan Zhao
2. Hanshuo Zhu
3. Jinqian Li
4. Li Sun

• Curated by eLife

  eLife assessment

  This important study shows that Type 3 secretion translocons in E. tarda and other bacteria activate the NAIP-NLRC4 inflammasome. The data from cellular and biochemical experiments showing that EseB is required for activation of the NLRC4 inflammasome are convincing, and comparing other translocons and additional cellular assays will provide further strength. This paper is broadly relevant to those investigating host-pathogen interactions in diverse organisms.

Reviewed by eLife

Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross stage recognition of glutamate-rich repeats

1. Axelle Amen
2. Randy Yoo
3. Amanda Fabra-García
4. Judith Bolscher
5. William JR Stone
6. Isabelle Bally
7. Sebastián Dergan-Dylon
8. Iga Kucharska
9. Roos M de Jong
10. Marloes de Bruijni
11. Teun Bousema
12. C Richter King
13. Randall S MacGill
14. Robert W Sauerwein
15. Jean-Philippe Julien
16. Pascal Poignard
17. Matthijs M Jore

• Curated by eLife

  eLife assessment

  This study reports important results and new insights into humoral immune responses to Plasmodium falciparum sexual stage proteins. The experiments are based on the use of target-agnostic memory B cell sorting and screening approaches as well as several state-of-the-art technologies. The authors present compelling evidence that one antibody, B1E11K, is cross-reactive with multiple proteins containing glutamate-rich repeats through homotypic interactions, a process similar to what has been observed for Plasmodium circumsporozoite protein repeat-directed antibodies.

Reviewed by eLife

Transposable elements may enhance antiviral resistance in HIV-1 elite controllers

1. Manvendra Singh
2. Sabrina M. Leddy
3. Luis Pedro Iñiguez
4. Matthew L. Bendall
5. Douglas F. Nixon
6. Cédric Feschotte

Reviewed by Review Commons

Heparan sulphate binding controls in vivo half-life of the HpARI protein family

1. Florent Colomb
2. Abhishek Jamwal
3. Adefunke Ogunkanbi
4. Tania Frangova
5. Alice R Savage
6. Sarah Kelly
7. Gavin J Wright
8. Matthew K Higgins
9. Henry J McSorley

• Curated by eLife

  eLife assessment

  This valuable study uses in vitro and in vivo methods to identify HpARI proteins from H. polygyrus as modulators of the host immune system. Based on comprehensive approaches for investigating differential roles of HpARI proteins, the data are solid, but there are some concerns whether the claims are fully validate. This paper is relevant to those who investigate host-pathogen interactions at the systems and molecular levels.

Reviewed by eLife

Functional Diversity of Memory CD8 T Cells is Spatiotemporally Imprinted

1. Miguel Reina-Campos
2. Alexander Monell
3. Amir Ferry
4. Vida Luna
5. Kitty P. Cheung
6. Giovanni Galletti
7. Nicole E. Scharping
8. Kennidy K. Takehara
9. Sara Quon
10. Brigid Boland
11. Yun Hsuan Lin
12. William H. Wong
13. Cynthia S. Indralingam
14. Gene W. Yeo
15. John T. Chang
16. Maximilian Heeg
17. Ananda W. Goldrath

Reviewed by preLights

One N-glycan regulates natural killer cell antibody-dependent cell-mediated cytotoxicity and modulates Fc γ receptor IIIa / CD16a structure

1. Paul G. Kremer
2. Elizabeth A. Lampros
3. Allison M. Blocker
4. Adam W. Barb

• Curated by eLife

  eLife assessment

  This study investigates the mechanistic connection between glycosylation at the N162 site of the Fc gamma receptor FcγRIIIa and the regulation of NK cell-mediated antibody-dependent cytotoxicity. The compelling findings, derived from novel isotope labeling approaches and state-of-the-art NMR spectroscopy techniques, underscore the impact of glycan composition on receptor stability and immune function. This research offers fundamental insights that could aid in the development of more effective therapeutic antibodies. The manuscript will be of interest to researchers in the fields of immunology and therapeutic antibody development.

Reviewed by eLife

Adipocyte microRNA-802 promotes adipose tissue inflammation and insulin resistance by modulating macrophages in obesity

1. Yue Yang
2. Bin Huang
3. Yimeng Qin
4. Danwei Wang
5. Yinuo Jin
6. Linmin Su
7. Yi Pan
8. Yanfeng Zhang
9. Yumeng Shen
10. Wenjun Hu
11. Zhengyu Cao
12. Liang Jin
13. Fangfang Zhang

• Curated by eLife

  eLife assessment

  This important study utilizes a comprehensive array of animal and cellular models, alongside various techniques, to elucidate the mechanism by which adipose tissue miR-802 contributes to inflammation and metabolic dysfunction in obesity. The data is solid, with clear, reproducible changes showing low variability among biological replicates and consistency across different models. However, some conclusions should be further substantiated with additional data to enhance the scope and strength of the manuscript.

Reviewed by eLife