Immunology

Identification of a Neuroimmune Circuit that Regulates Allergic Inflammation in the Esophagus

1. Kendall Kellerman
2. Mia Natale
3. Eddie Gerstner
4. Yrina Rochman
5. Mark Rochman
6. Michael P. Jankowski
7. Marc E. Rothenberg

Reviewed by Arcadia Science

Mast cells promote pathology and susceptibility in tuberculosis

1. Ananya Gupta
2. Vibha Taneja
3. Javier Rangel Moreno
4. Abhimanyu
5. Mushtaq Ahmed
6. Nilofer Naqvi
7. Kuldeep S Chauhan
8. Daniela Trejo-Ponce de León
9. Gustavo Ramírez-Martínez
10. Luis Jiménez-Alvarez
11. Cesar Luna-Rivero
12. Joaquin Zuniga
13. Deepak Kaushal
14. Shabaana A Khader

• Curated by eLife

  eLife Assessment

  In this useful study, the authors use published scRNA-seq data to highlight the importance of mast cells (MCs) in TB granulomas, reporting a comparative assessment of chymase- and tryptase-expressing MCs in the lungs of tuberculosis-infected individuals and non-human primates, with MC-deficient mice showing reduced lung bacterial burden and pathology during infection. Whilst the findings are helpful, the evidence to support conclusions is inconsistent across models and thus incomplete. Specifically, the data supporting a role for MCs in coordinating cytokine responses to modulate pathology, susceptibility to tuberculosis, and dissemination during infection are weak.

Reviewed by eLife, Arcadia Science

Proximity labeling defines the phagosome lumen proteome of murine and primary human macrophages

1. Benjamin L. Allsup
2. Supriya Gharpure
3. Bryan D. Bryson

Reviewed by Arcadia Science

NK Cell Exhaustion in Wilson’s Disease Revealed by Single-cell RNA Sequencing Predicts the Prognosis of Cholecystitis

1. Yong Jin
2. Jiayu Xing
3. Chenyu Dai
4. Lei Jin
5. Wanying Zhang
6. Qianqian Tao
7. Mei Hou
8. Ziyi Li
9. Wen Yang
10. Qiyu Feng
11. Hongyang Wang
12. Qingsheng Yu

• Curated by eLife

  eLife Assessment

  This study presents valuable findings, based on solid methods, to link metabolic dysfunction in Wilson's disease to immune cell dysregulation and poor cholecystitis outcomes. The integration of clinical data and single-cell analyses highlights NK cell exhaustion as a key factor, offering insights with potential therapeutic implications. The work will be of interest to colleagues in inflammatory and metabolic diseases.

Reviewed by eLife

PD-L1+ Neutrophils mediate Susceptibility during Systemic Inflammatory Response in Non-Alcoholic Fatty Liver Disease

1. Cleyson da Cruz Oliveira Barros
2. Alexandre Kanashiro
3. Gabriel Victor Lucena da Silva
4. Guilherme Cesar Martelossi Cebinelli
5. Luiz Osório Leiria
6. Thiago Mattar Cunha
7. José Carlos Alves Filho
8. Fernando Queiroz Cunha

• Curated by eLife

  eLife Assessment

  This important study highlights the key role of NK cells and PD-L1+ neutrophils in worsening sepsis responses in the context of of MASH (metabolic dysfunction-associated steatohepatitis). While the data are solid, the overall evidence for the role of neutrophils in mediating this effect, which is based on a choline-deficient high-fat diet model of various knockouts or selective ablation of immune cell types, remains incomplete. The study will be of interest to researchers in immunopathological disease mechanisms.

Reviewed by eLife

Altered hepatic metabolism mediates sepsis preventive effects of reduced glucose supply in infected preterm newborns

1. Ole Bæk
2. Tik Muk
3. Ziyuan Wu
4. Yongxin Ye
5. Bekzod Khakimov
6. Alessandra Maria Casano
7. Bagirath Gangadharan
8. Ivan Bilic
9. Anders Brunse
10. Per Torp Sangild
11. Duc Ninh Nguyen

• Curated by eLife

  eLife Assessment

  The study follows up on previous work suggesting that lower glucose concentrations are protective from sepsis but put the patient at risk for hypoglycemia. In this paper, the authors identify that a slightly higher dose of glucose is still protective but no longer puts the patients at risk for hypoglycemia. The study is important, supported by convincing data, and will be of interest to a broad audience.

Reviewed by eLife

Syngeneic natural killer cell therapy activates dendritic and T cells in metastatic lungs and effectively treats low-burden metastases

1. Shih-Wen Huang
2. Yein-Gei Lai
3. Hao-Ting Liao
4. Chin-Ling Chang
5. Ruo-Yu Ma
6. Yung-Hsiang Chen
7. Yae-Huei Liou
8. Zhen-Qi Wu
9. Yu-Chen Wu
10. Ko-Jiunn Liu
11. Yen-Tsung Huang
12. Jen-Lung Yang
13. Ming-Shen Dai
14. Nan-Shih Liao

• Curated by eLife

  eLife Assessment

  In this important study the authors develop an elegant lung metastasis mouse model that closely mimics the events in human patients. They provide convincing evidence for the effectiveness of IL-15/12-conditioned NK cells in this design, which was also critical for the authors being able to conclusively reveal the T cell-dependency of NK-cell-mediated long-term control of experimental metastasis. Of note, an investigator-initiated clinical trial demonstrated that similar NK cell infusions in cancer patients after resections were safe and showed signs of efficacy, which is of promising clinical application value.

Reviewed by eLife

TCR transgenic clone selection guided by immune receptor analysis and single cell RNA expression of polyclonal responders

1. Nincy Debeuf
2. Sahine Lameire
3. Manon Vanheerswynghels
4. Julie Deckers
5. Caroline De Wolf
6. Wendy Toussaint
7. Rein Verbeke
8. Kevin Verstaen
9. Hamida Hammad
10. Stijn Vanhee
11. Bart N Lambrecht

• Curated by eLife

  eLife Assessment

  The paper illustrates a valuable approach to generating TCR transgenic mice specific for known epitopes. Solid evidence validates the described pipeline for identification of TCRs from single-cell datasets for the generation of TCR transgenic mice, while obviating the need for generation of T-cell lines and hybridomas.

Reviewed by eLife

Tumor-specific antibodies elicited by engineered bacteria promote bladder cancer immunotherapy

1. Mathieu Rouanne
2. Noah Chen
3. Dylan L. Mariuzza
4. Fangda Li
5. Kenia de los Santos-Alexis
6. Thomas M. Savage
7. Rosa L. Vincent
8. Cathy L. Mendelsohn
9. Tal Danino
10. Nicholas Arpaia

Reviewed by PREreview

CD131 Contributes to Ulcerative Colitis Pathogenesis by Promoting Macrophage Infiltration

1. Zhiyuan Wu
2. Lindi Liu
3. Chenchen He
4. Lin Xiao
5. Duo Yun
6. Junliang Chen
7. Zhihao Liu
8. Wenjun Li
9. Qingjie Lv
10. Xiaodong Tan

• Curated by eLife

  eLife Assessment

  Ulcerative colitis (UC) is a chronic gut inflammatory condition affecting the colon in humans. This study uses human samples as well as a mouse model of colitis induced by a chemical, DSS, to investigate the role of an immune marker, CD131, in UC pathogenesis. The study, as presented, is incomplete, as experimental details are lacking, the statistical analyses are deficient, and there is not yet direct evidence for a CD131-mediated mechanism of gut inflammation.

Reviewed by eLife