1. High-speed whole-genome sequencing of a Whippet: Rapid chromosome-level assembly and annotation of an extremely fast dog’s genome

    This article has 8 authors:
    1. Marcel Nebenführ
    2. David Prochotta
    3. Alexander Ben Hamadou
    4. Axel Janke
    5. Charlotte Gerheim
    6. Christian Betz
    7. Carola Greve
    8. Hanno Jörn Bolz
    This article has been curated by 1 group:
    • Curated by GigaByte

      Editors Assessment:

      This Data Release paper presents the genome of the whippet breed of dog. Demonstrating a streamlined laboratory and bioinformatics workflows with PacBio HiFi long-read whole-genome sequencing that enables the generation of a high-quality reference genome within one week. The genome study being a collaboration between an academic biodiversity institute and a medical diagnostic company. The presented method of working and workflow providing examples that can be used for a wide range of future human and non-human genome projects. The final is 2.47 Gbp assembly being of high quality - with a contig N50 of 55 Mbp and a scaffold N50 of 65.7 Mbp. This reference being scaffolded into 39 chromosome-length scaffolds and the annotation resulting in 28,383 transcripts. The results also looked at the Myostatin gene which can be used for breeding purposes, as these heterozygous animals can have an advantage in dog races. The reviewers making the authors clarify this part a little better with additional results. Overall this study demonstrating how rapidly animal genome research can be carried out through close and streamlined time management and collaboration.

      This evaluation refers to version 1 of the preprint

    Reviewed by GigaByte

    This article has 2 evaluationsAppears in 2 listsLatest version Latest activity
  2. Transcriptomic profiling of Schlemm’s canal cells reveals a lymphatic-biased identity and three major cell states

    This article has 15 authors:
    1. Revathi Balasubramanian
    2. Krishnakumar Kizhatil
    3. Taibo Li
    4. Nicholas Tolman
    5. Aakriti Bhandari
    6. Graham Clark
    7. Violet Bupp-Chickering
    8. Ruth A Kelly
    9. Sally Zhou
    10. John Peregrin
    11. Marina Simón
    12. Christa Montgomery
    13. W Daniel Stamer
    14. Jiang Qian
    15. Simon WM John
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study has characterized the unique expression of Schlemm's canal endothelial cells (SECs) using FACS-sorted specific cell bulk RNA-Seq and scRNA-/snRNA-Seq of mouse SECs. The compelling study identified novel biomarkers for SECs and molecular markers for two inner wall SEC states and outwall SECs in mouse eyes. Significant gene networks and pathways were elucidated for their potential contribution to glaucoma pathogenesis, providing targets for further research in relation to glaucoma.

    Reviewed by eLife

    This article has 8 evaluationsAppears in 1 listLatest version Latest activity
  3. Interchromosomal segmental duplication drives translocation and loss of P. falciparum histidine-rich protein 3

    This article has 11 authors:
    1. Nicholas J Hathaway
    2. Isaac E Kim
    3. Neeva WernsmanYoung
    4. Sin Ting Hui
    5. Rebecca Crudale
    6. Emily Y Liang
    7. Christian P Nixon
    8. David Giesbrecht
    9. Jonathan J Juliano
    10. Jonathan B Parr
    11. Jeffrey A Bailey
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This work provides important insight into the mechanisms of hrp2 and particularly hrp3 deletion generation. The generation of additional long-read data alongside a new analysis of 19,000 public short-read sequenced genomes makes this the most detailed investigation currently available on this topic, which has high public health importance and also basic biological interest. The revised version of the manuscript provides convincing evidence for the proposed mechanisms.

    Reviewed by eLife

    This article has 9 evaluationsAppears in 1 listLatest version Latest activity
  4. Genome-wide mapping of native co-localized G4s and R-loops in living cells

    This article has 8 authors:
    1. Ting Liu
    2. Xing Shen
    3. Yijia Ren
    4. Hongyu Lu
    5. Yu Liu
    6. Chong Chen
    7. Lin Yu
    8. Zhihong Xue
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study describes a useful antibody-free method to map both G-quadruplexes and R-loops in vertebrate cells independently of the BG4 and S9.6 antibodies. It also reveals that the helicase Dhx9 can affect the self-renewal and differentiation capacities of mESCs, perhaps by regulating co-localized G4s and R-loops. The datasets provided might constitute a good starting point for future functional studies, and although the strength of the evidence that DHX9 interferes with the ability of mESCs to differentiate by regulating directly the stability of either G4s or R-loops has been improved compared to a previous version, it is still incomplete.

    Reviewed by eLife

    This article has 10 evaluationsAppears in 1 listLatest version Latest activity
  5. Regulatory genome annotation of 33 insect species

    This article has 8 authors:
    1. Hasiba Asma
    2. Ellen Tieke
    3. Kevin D Deem
    4. Jabale Rahmat
    5. Tiffany Dong
    6. Xinbo Huang
    7. Yoshinori Tomoyasu
    8. Marc S Halfon
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      In the revised version of this important study, the authors present a convincing pipeline for insect genome regulatory annotation across 33 insect genomes spanning 5 orders. Despite technical limitations in the field owing to the lack of comprehensive knowledge of enhancer content in any system, the authors employ several independent downstream analyses to support the validity of their enhancer predictions for a subset of these genomes. Taken together, the revised results suggest that this prediction pipeline may have uses in identifying functional enhancers across large phylogenetic distances. Reviewers note caveats that an experimental validation is not yet available in the field to validate a large class of newly identified enhancers across such evolutionary distances, and other pipelines might be of use to compare. This work will be of interest to the computational genomics, evolutionary biology, and gene regulation fields.

    Reviewed by eLife

    This article has 9 evaluationsAppears in 1 listLatest version Latest activity
  6. Robustness and reliability of single-cell regulatory multi-omics with deep mitochondrial mutation profiling

    This article has 3 authors:
    1. Chen Weng
    2. Jonathan S. Weissman
    3. Vijay G. Sankaran

    Reviewed by Arcadia Science

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  7. Essentiality and dynamic expression of the human tRNA pool during viral infection

    This article has 5 authors:
    1. Noa Aharon-Hefetz
    2. Michal Schwartz
    3. Orna Dahan
    4. Noam Stern-Ginossar
    5. Yitzhak Pilpel

    Reviewed by Review Commons

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  8. Identification of novel myelodysplastic syndromes prognostic subgroups by integration of inflammation, cell-type composition, and immune signatures in the bone marrow

    This article has 13 authors:
    1. Sila Gerlevik
    2. Nogayhan Seymen
    3. Shan Hama
    4. Warisha Mumtaz
    5. I Richard Thompson
    6. Seyed R Jalili
    7. Deniz E Kaya
    8. Alfredo Iacoangeli
    9. Andrea Pellagatti
    10. Jacqueline Boultwood
    11. Giorgio Napolitani
    12. Ghulam J Mufti
    13. Mohammad M Karimi
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This manuscript uses public datasets of myelodysplastic syndrome (MDS) patients to undertake a multi-omics analysis of clinical, genomic, and transcriptomic datasets. Useful findings are provided by way of interesting correlations of specific mutations with inflammation and differing clinical outcomes. The evidence is solid and interesting, and the manuscript is of substantive value to hematologists and clinical immunologists.

    Reviewed by eLife

    This article has 6 evaluationsAppears in 1 listLatest version Latest activity
  9. A rapid phylogeny-based method for accurate community profiling of large-scale metabarcoding datasets

    This article has 2 authors:
    1. Lenore Pipes
    2. Rasmus Nielsen
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This potentially important work presents a tool for performing phylogenetic taxonomic classification of DNA sequences. In terms of methodology, the work is compelling. The authors perform a benchmark experiment against current state-of-the-art tools using real and simulated datasets to demonstrate where the novel tool stands in the context of existing methods. However, the experimentation is still incomplete. It would benefit from a more thorough exploration of existing methods as well as data sets that better represent real-world use cases.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  10. Feature sequence-based genome mining uncovers the hidden diversity of bacterial siderophore pathways

    This article has 14 authors:
    1. Shaohua Gu
    2. Yuanzhe Shao
    3. Karoline Rehm
    4. Laurent Bigler
    5. Di Zhang
    6. Ruolin He
    7. Ruichen Xu
    8. Jiqi Shao
    9. Alexandre Jousset
    10. Ville-Petri Friman
    11. Xiaoying Bian
    12. Zhong Wei
    13. Rolf Kümmerli
    14. Zhiyuan Li
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study presents a novel pipeline for the large-scale genomic prediction of members of the non-ribosomal peptide group of pyoverdines based on a dataset from nearly 2000 Pseudomonas genomes. The advance presented in this study is based on convincing evidence. This study of bacterial siderophores has broad theoretical and practical implications beyond a singular subfield.

    Reviewed by eLife

    This article has 8 evaluationsAppears in 1 listLatest version Latest activity
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