Genetics

High-throughput library transgenesis in Caenorhabditis elegans via Transgenic Arrays Resulting in Diversity of Integrated Sequences (TARDIS)

1. Zachary C Stevenson
2. Megan J Moerdyk-Schauwecker
3. Stephen A Banse
4. Dhaval S Patel
5. Hang Lu
6. Patrick C Phillips

• Curated by eLife

  eLife assessment

  This manuscript provides a description of an approach for efficiently integrating diverse libraries into the C. elegans genome and tools that enable researchers to use the method. It is a valuable contribution for researchers carrying out experiments that would benefit from easy generation of such libraries, and the data for the effectiveness of the method is solid. The advantages of this approach in terms of ease and effectiveness relative to others with similar aims will emerge as they are put to more general use in addressing biological problems.

Reviewed by eLife, Arcadia Science

Cas9-induced nonhomologous recombination in C. elegans

1. Stefan Zdraljevic
2. Laura Walter-McNeill
3. Heriberto Marquez
4. Leonid Kruglyak

Reviewed by Arcadia Science

Disparate patterns of genetic divergence in three widespread corals across a pan-Pacific environmental gradient highlights species-specific adaptation trajectories

1. Benjamin C C Hume
2. Christian R Voolstra
3. Eric Armstrong
4. Guinther Mitushasi
5. Barbara Porro
6. Nicolas Oury
7. Sylvain Agostini
8. Emilie Boissin
9. Julie Poulain
10. Quentin Carradec
11. David A. Paz-García
12. Didier Zoccola
13. Hélène Magalon
14. Clémentine Moulin
15. Guillaume Bourdin
16. Guillaume Iwankow
17. Sarah Romac
18. Bernard Banaigs
19. Emmanuel Boss
20. Chris Bowler
21. Colomban de Vargas
22. Eric Douville
23. Michel Flores
24. Paola Furla
25. Pierre E Galand
26. Eric Gilson
27. Fabien Lombard
28. Stéphane Pesant
29. Stéphanie Reynaud
30. Matthew B. Sullivan
31. Shinichi Sunagawa
32. Olivier Thomas
33. Romain Troublé
34. Rebecca Vega Thurber
35. Patrick Wincker
36. Serge Planes
37. Denis Allemand
38. Didier Forcioli

Reviewed by Arcadia Science

Rescuable sleep and synaptogenesis phenotypes in a Drosophila model of O-GlcNAc transferase intellectual disability

1. Ignacy Czajewski
2. Bijayalaxmi Swain
3. Jiawei Xu
4. Laurin McDowall
5. Andrew T Ferenbach
6. Daan MF van Aalten

Reviewed by Review Commons

Dietary Restriction Impacts Peripheral Circadian Clock Output Important for Longevity in Drosophila

1. Dae-Sung Hwangbo
2. Yong-Jae Kwon
3. Marta Iwanaszko
4. Peng Jiang
5. Ladan Abbasi
6. Nicholas Wright
7. Sarayu Alli
8. Alan L. Hutchison
9. Aaron R. Dinner
10. Rosemary I Braun
11. Ravi Allada

• Curated by eLife

  eLife assessment

  This study describes important findings on how a core component of the circadian clock impacts the effect of dietary restriction (DR) on longevity and fecundity in Drosophila, which lead the authors to postulate rhythmic control of proteostasis in the fat body as a critical aspect of DR effects. The evidence presented is still incomplete, not fully supporting the conclusions of the study, as alternative hypotheses/explanations have not yet been systematically explored. The work will nevertheless be of substantial interest to researchers working in circadian and cell biology, metabolism, and aging, with an interesting hypothesis to be explored further.

Reviewed by eLife

Decoding the genetic and chemical basis of sexual attractiveness in parasitic wasps

1. Weizhao Sun
2. Michelle Ina Lange
3. Jürgen Gadau
4. Jan Buellesbach

• Curated by eLife

  eLife assessment

  This important study reveals the genetic regulation of changes in cuticular hydrocarbon profiles in a Hymenopteran insect and links these changes with courtship behaviour and sexual attractiveness. It provides convincing empirical evidence, spanning genetic, chemical, and behavioural data. It adds valuable new perspectives on the mechanisms that underlie chemical recognition and communication systems in nature.

Reviewed by eLife

Long-range DNA end resection supports homologous recombination by checkpoint activation rather than extensive homology generation

1. Michael T Kimble
2. Matthew J Johnson
3. Mattie R Nester
4. Lorraine S Symington

• Curated by eLife

  eLife assessment:

  This fundamental study provides compelling evidence that long-range resection is important for recombination between distal, but not proximal, homologous sequences. It is thus proposed that a major role of long resection of a double-strand break mediated by Sgs1 and Exo1 is to activate the DNA damage checkpoint to allow the chromosomal mobility needed for the DNA ends to find a distant homologous sequence with which repair via homologous recombination, adding a new biological meaning to the role of long DNA resection in homologous recombination.

Reviewed by eLife

Ether lipid biosynthesis promotes lifespan extension and enables diverse pro-longevity paradigms in Caenorhabditis elegans

1. Lucydalila Cedillo
2. Fasih M Ahsan
3. Sainan Li
4. Nicole L Stuhr
5. Yifei Zhou
6. Yuyao Zhang
7. Adebanjo Adedoja
8. Luke M Murphy
9. Armen Yerevanian
10. Sinclair Emans
11. Khoi Dao
12. Zhaozhi Li
13. Nicholas D Peterson
14. Jeramie Watrous
15. Mohit Jain
16. Sudeshna Das
17. Read Pukkila-Worley
18. Sean P Curran
19. Alexander A Soukas

• Curated by eLife

  eLife assessment

  Using C. elegans as a model organism, the study hones in on the role of ether lipid biosynthesis as an effector of metformin--a process previously implicated in extending lifespan in response to diet--, TOR signalling, and mitochondrial interventions. The data in this paper are compelling, and a better understanding of biguanide impact on metabolism is highly important in the field.

Reviewed by eLife

Genes associated with liver damage signalling pathways may impact the severity of COVID-19 symptoms in Spanish and Italian populations

1. Leire Moya
2. Samaneh Farashi
3. Prashanth N Suravajhala
4. Panchadsaram Janaththani
5. Jyotsna Batra

Reviewed by ScreenIT

Mutational signatures and heterogeneous host response revealed via large-scale characterization of SARS-CoV-2 genomic diversity

1. Alex Graudenzi
2. Davide Maspero
3. Fabrizio Angaroni
4. Rocco Piazza
5. Daniele Ramazzotti

Reviewed by ScreenIT