Developmental Biology

Immune–Vascular intercellular signalling coordinates tissue plasticity during adult muscle repair in Drosophila

1. Nourhene Ammar
2. Olivier Josse
3. Aurélien Guillou
4. Jessica Perochon
5. Hadi Boukhatmi

Reviewed by preLights

Cross-species insemination reveals mouse sperm ability to enter and cross the fish micropyle

1. Suma Garibova
2. Eva Stickler
3. Fatima AlAli
4. Maha A Abdulla
5. Abbirami Sathappan
6. Sahar I Da'as
7. Lillian Ghanem
8. Mohamed Nadhir Djekidel
9. Rick Portman
10. Matteo Avella

• Curated by eLife

  eLife Assessment

  This important study reports the conservation of sperm-egg envelope binding by demonstrating successful recognition of the micropyle in fish eggs by mouse sperm. The evidence supporting the conclusions drawn is convincing. This study will be of interest to reproductive biologists and clinicians studying the biology of fertilization and fertility.

Reviewed by eLife

Actin cytoskeletal deregulation, caused by RhoGEF2 overexpression, induces cell competition dependent on Ptp10D, Crumbs, and the Hippo signaling pathway

1. Natasha Fahey-Lozano
2. Marta Portela
3. John E. La Marca
4. Helena E. Richardson

Reviewed by Review Commons

Gene regulatory dynamics during craniofacial development in a carnivorous marsupial

1. Laura E Cook
2. Charles Y Feigin
3. John D Hills
4. Davide M Vespasiani
5. Andrew J Pask
6. Irene Gallego Romero

• Curated by eLife

  eLife Assessment

  This important study of regulatory elements and gene expression in the craniofacial region of the fat-tailed dunnart shows that, compared to placental mammals, marsupial craniofacial tissue develops in a precocious manner, with enhancer regulatory elements as primary driver of this difference. The compelling data, including a new dunnart genome assembly, provide an invaluable reference for future mammalian evolution studies, especially once additional developmental time point for the fat-tailed dunnart become available.

Reviewed by eLife

Asynchronous mouse embryo polarization leads to heterogeneity in cell fate specification

1. Adiyant Lamba
2. Meng Zhu
3. Maciej Meglicki
4. Sylwia Czukiewska
5. Lakshmi Balasubramaniam
6. Ron Hadas
7. Nina Weishaupt
8. Ekta M Patel
9. Yu Hua Kavanagh
10. Ran Wang
11. Naihe Jing
12. Magdalena Zernicka-Goetz

• Curated by eLife

  eLife Assessment

  This fundamental work substantially advances our understanding of the molecular basis by which early symmetry breaking events connect to the following cell fate specifications in preimplantation mammalian embryos. The evidence supporting the conclusions is compelling, with advanced image based assays and microinjection based functional tests. The work will be of broad interest to cell and developmental biologists.

Reviewed by eLife

Dissecting infant leukemia developmental origins with a hemogenic gastruloid model

1. Denise Ragusa
2. Chun Wai Suen
3. Gabriel Torregrosa Cortes
4. Fabio Pastorino
5. Ayona Johns
6. Ylenia Cicirò
7. Liza Dijkhuis
8. Susanne van den Brink
9. Michele Cilli
10. Connor Byrne
11. Giulia-Andreea Ionescu
12. Joana Cerveira
13. Kamil R Kranc
14. Victor Hernandez-Hernandez
15. Mirco Ponzoni
16. Anna Bigas
17. Jordi Garcia-Ojalvo
18. Alfonso Martínez Arias
19. Cristina Pina

• Curated by eLife

  eLife Assessment

  This valuable study presents a mouse gastruloid system to generate successive waves of hematopoietic progenitors that in vivo would emerge during embryonic development. Although this newly revised manuscript has addressed some of the concerns raised during the first round of review, the study is still considered incomplete, as the claims are only partially supported. In particular, the claim of definitive wave hematopoietic progenitors being produced in the gastruloids, and their engraftment after transplantation, would benefit from further validation.

Reviewed by eLife

Comprehensive profiling of migratory primordial germ cells reveals niche-specific differences in non-canonical Wnt and Nodal-Lefty signaling in anterior vs posterior migrants

1. Rebecca Garrett Jaszczak
2. Jay W Zussman
3. Daniel E Wagner
4. Diana J Laird

• Curated by eLife

  eLife Assessment

  This revised study provides fundamental insights into the differences in migratory primordial germ cells based on their anterior or posterior location. Through convincing methodology and analysis of single-cell RNA sequencing of an exceptionally large number of migratory primordial germ cells and surrounding somatic cells, the novel findings and datasets generated from this study provide many hypotheses of interest to germ cell biologists.

Reviewed by eLife

TET knockout cells transit between pluripotent states and exhibit precocious germline entry

1. Raphaël Pantier
2. Elisa Barbieri
3. Sara Gonzalez Brito
4. Ella Thomson
5. Tülin Tatar
6. Douglas Colby
7. Man Zhang
8. Ian Chambers

Reviewed by preLights

Synergistic and independent roles for Nodal and FGF in zebrafish cardiac progenitor cell migration and asymmetric heart morphogenesis

1. Vanessa Gonzalez
2. Meagan G. Grant
3. Makoto Suzuki
4. Briana Christophers
5. Jessica Rowland Williams
6. Rebecca D. Burdine

Reviewed by preLights

Gene regulatory mechanisms guiding bifurcation of inhibitory and excitatory neuron lineages in the mouse anterior brainstem

1. Sami Kilpinen
2. Lassi Virtanen
3. Silvana Bodington Celma
4. Amos Bonsdorff
5. Heidi Heliölä
6. Kaia Achim
7. Juha Partanen

• Curated by eLife

  eLife Assessment

  This work is a important resource for hypothesis testing of candidate upstream transcriptional regulatory factors that control the spatiotemporal expression of selector genes and their targets for GABAergic vs glutamatergic neuron fate in the anterior brainstem. Extensive high-quality datasets were generated and state of the art computational methods were convincingly implemented to identify candidate regulatory elements. The work will be of interest to biologists working to understand neuronal gene regulatory networks.

Reviewed by eLife