Developmental Biology

Cytoneme-mediated intercellular signaling in keratinocytes is essential for epidermal remodeling in zebrafish

1. Yi Wang
2. Thomas Nguyen
3. Qingan He
4. Oliver Has
5. Kiarash Forouzesh
6. Dae Seok Eom

• Curated by eLife

  eLife Assessment

  This is a valuable study showing that differentiated cells of the zebrafish skin form membrane protrusions called cytonemes that contact and likely transmit Notch signals to cells of the undifferentiated layer below. The data are convincing that cytoneme like protrusions from the periderm are required for proper periderm structure, proliferation, gene expression, and Notch signaling. Evidence that inflammatory signaling through IL-17 affects epidermal differentiation, Notch and cytoneme formation is solid, but whether these are through a single common or two parallel pathways requires further investigation.

Reviewed by eLife

Genetic compensation in podocalyxin-like mutants during zebrafish liver development

1. Alexis N Ross
2. Natalie M Miscik
3. Sharanya Maanasi Kalasekar
4. James D Harris
5. Mimi Tran
6. Aavrati Saxena
7. Steven Andrew Baker
8. Kimberley Jane Evason

• Curated by eLife

  eLife Assessment

  The manuscript by Ross, Miscik, and others describes an intriguing series of observations made when investigating the requirement for podxl during hepatic development in zebrafish. Understanding how genetic compensation pathways are involved in gene function is an important question. However, there is incomplete evidence provided in the manuscript at this point to conclude that discrepancies between observed phenotypes are due to genetic compensation.

Reviewed by eLife

The essential role of connective-tissue cells during axolotl limb regeneration

1. Damián García-García
2. Dunja Knapp
3. Minjoo Kim
4. Katelyn Jamwal
5. Heath Fuqua
6. Ryan P. Seaman
7. Riley E. Grindle
8. Sergej Nowoshilow
9. Maria Novatchkova
10. Fred W. Kolling
11. Joel H. Graber
12. Prayag Murawala

Reviewed by preLights

Alteration of long- and short-term hematopoietic stem cell ratio causes myeloid-biased hematopoiesis

1. Katsuyuki Nishi
2. Taro Sakamaki
3. Akiomi Nagasaka
4. Kevin Shuolong Kao
5. Kay Sadaoka
6. Masahide Asano
7. Nobuyuki Yamamoto
8. Akifumi Takaori-Kondo
9. Masanori Miyanishi

• Curated by eLife

  eLife Assessment

  This manuscript provides valuable insights into the heterogeneity of hematopoietic stem cells and age-associated myeloid-biased hematopoiesis. While several aspects of the study are intriguing and merit further investigation, the current results remain incomplete and additional data are necessary to substantiate the conclusions. Some of the methods and data analyses partially support the claims.

Reviewed by eLife, preLights

FGF and MafB regulated cadherin expression drives lamina formation in the auditory hindbrain

1. Rosanna CG Smith
2. Maryam Clark
3. Mireya Vazquez-Prada
4. Marc Astick
5. Kristina C Tubby
6. Stephen R Price

• Curated by eLife

  eLife Assessment

  The authors conducted a valuable study that investigates a molecular pathway mediating the transformation of a cell aggregate into a sheet known as the nucleus laminaris, a crucial site for auditory processing. While the study offers a comprehensive view of the sequence of developmental events and suggests possible roles for FGF signaling, the transcription factor Mafb, and the cell surface adhesive molecule Cadherin-23 in this process, the current data were considered incomplete.

Reviewed by eLife

Cardiac fibroblasts regulate myocardium and coronary vasculature development in the murine heart via the collagen signaling pathway

1. Yiting Deng
2. Yuanhang He
3. Juan Xu
4. Haoting He
5. Manling Zhang
6. Guang Li

• Curated by eLife

  eLife Assessment

  This study provides a comprehensive analysis of gene expression and bioinformatics data, offering important insights into the roles of fibroblasts in cardiac development. The large and well-analyzed single-cell RNA sequencing (scRNA-seq) dataset is compelling and a significant contribution to the field, and will be of broad interest to the scientific community.

Reviewed by eLife

The gut contractile organoid for studying the gut motility regulated by coordinating signals between interstitial cells of Cajal and smooth muscles

1. Rei Yagasaki
2. Ryo Nakamura
3. Yuuki Shikaya
4. Ryosuke Tadokoro
5. Ruolin Hao
6. Zhe Wang
7. Mototsugu Eiraku
8. Masafumi Inaba
9. Yoshiko Takahashi

• Curated by eLife

  eLife Assessment

  This valuable study reports the development of a novel organoid system for studying the emergence of autorhythmic gut peristaltic contractions through the interaction between interstitial cells of Cajal and smooth muscle cells. The authors further utilized the system to provide convincing evidence for a previously unappreciated potential role for smooth muscle cells in regulating the firing rate of interstitial cells of Cajal. The work will be of interest to those studying development and physiology of the gut.

Reviewed by eLife

Decoding protein phosphorylation during oocyte meiotic divisions using phosphoproteomics

1. Leonid Peshkin
2. Enrico maria Daldello
3. Elizabeth S Van Itallie
4. Matthew Sonnett
5. Johannes Kreuzer
6. Wilhelm Haas
7. Marc W Kirschner
8. Catherine Jessus

• Curated by eLife

  eLife Assessment

  This important paper describes a comprehensive quantitative phospho-proteomic analysis of the meiotic progression of Xenopus oocytes. Using time-resolved proteomic analyses, the authors provide insights into changes in protein levels and phosphorylation states to an unprecedented depth, quality, and quantitative detail. The key findings are compelling and offer a helpful resource for the scientific community.

Reviewed by eLife

PRDM16 functions as a co-repressor in the BMP pathway to suppress neural stem cell proliferation

1. Li He
2. Jiayu Wen
3. Qi Dai

• Curated by eLife

  eLife Assessment

  This valuable work presents how PRDM16 plays a critical role during colloid plexus development, through regulating BMP signaling. Solid evidence supports the context-dependent gene regulatory mechanisms both in vivo and in vitro. The work will be of broad interest to researchers working on growth factor signaling mechanisms and vertebrate development.

Reviewed by eLife

NR2F2 is required in the embryonic testis for fetal Leydig cell development

1. Aitana Perea-Gomez
2. Natividad Bellido Carreras
3. Magali Dhellemmes
4. Furong Tang
5. Coralie Le Gallo
6. Marie-Christine Chaboissier

Reviewed by eLife