SARS-CoV-2 productively infects human gut enterocytes

  1. Mart M. Lamers
  2. Joep Beumer
  3. Jelte van der Vaart
  4. Kèvin Knoops
  5. Jens Puschhof
  6. Tim I. Breugem
  7. Raimond B. G. Ravelli
  8. J. Paul van Schayck
  9. Anna Z. Mykytyn
  10. Hans Q. Duimel
  11. Elly van Donselaar
  12. Samra Riesebosch
  13. Helma J. H. Kuijpers
  14. Debby Schipper
  15. Willine J. van de Wetering
  16. Miranda de Graaf
  17. Marion Koopmans
  18. Edwin Cuppen
  19. Peter J. Peters
  20. Bart L. Haagmans
  21. Hans Clevers

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Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes an influenza-like disease with a respiratory transmission route; however, patients often present with gastrointestinal symptoms such as diarrhea, vomiting, and abdominal pain. Moreover, the virus has been detected in anal swabs, and cells in the inner-gut lining express the receptor that SARS-CoV-2 uses to gain entry to cells. Lamers et al. used human intestinal organoids, a “mini-gut” cultured in a dish, to demonstrate that SARS-CoV-2 readily replicates in an abundant cell type in the gut lining—the enterocyte—resulting in the production of large amounts of infective virus particles in the intestine. This work demonstrates that intestinal organoids can serve as a model to understand SARS-CoV-2 biology and infectivity in the gut.

Science , this issue p. 50

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  1. Version published to 10.1126/science.abc1669
  2. ScreenIT

    SciScore for 10.1101/2020.04.25.060350: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    No key resources detected.

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

    Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 11. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

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  3. Version published to 10.1101/2020.04.25.060350v1 on bioRxiv