Human iPSC-Derived Cardiomyocytes Are Susceptible to SARS-CoV-2 Infection

  1. Arun Sharma
  2. Gustavo Garcia
  3. Yizhou Wang
  4. Jasmine T. Plummer
  5. Kouki Morizono
  6. Vaithilingaraja Arumugaswami
  7. Clive N. Svendsen

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Abstract

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  1. Version published to 10.1016/j.xcrm.2020.100052
  2. ScreenIT

    SciScore for 10.1101/2020.04.21.051912: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementIRB: HiPSC Derivation: The appropriate institutional review board (IRB) and stem cell research oversight committee (SCRO) were consulted at UCLA and Cedars-Sinai Medical Center.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    The following antibodies and dilutions were used: α-actinin (1:100, Sigma-Aldrich Cat# A7811, RRID: AB_476766); cardiac troponin T (cTnT, 1:100, Abcam Cat# ab45932, RRID: AB_956386); SARS-CoV-2 spike (S) protein (1:100, BEI Resources NR-616 Monoclonal Anti-SARS-CoV S Protein (Similar to 240C) SARS coronavirus); SARS-CoV-2 double stranded RNA (1:100, J2 clone; Absolute Antibody Inc.); cleaved caspase-3 (1:200, Cell Signaling Technology Cat# 9661, RRID: AB_2341188).
    The
    detected: (Sigma-Aldrich Cat# A7811, RRID:AB_476766)
    cardiac T
    detected: (Abcam Cat# ab45932, RRID:AB_956386)
    Anti-SARS-CoV S Protein
    suggested: None
    cleaved caspase-3
    detected: (Cell Signaling Technology Cat# 9661, RRID:AB_2341188)
    Antibodies were donkey anti-rabbit 488 (Thermo Fisher Scientific Cat# R37118, RRID: AB_2556546), goat anti-mouse 555 (Thermo Fisher Scientific Cat# A32727, RRID: AB_2633276), and donkey anti-mouse 594 (Thermo Fisher Scientific Cat# R37115, RRID: AB_2556543).
    donkey anti-rabbit
    detected: (Thermo Fisher Scientific Cat# R37118, RRID:AB_2556546)
    anti-mouse
    detected: (Thermo Fisher Scientific Cat# R37115, RRID:AB_2556543)
    goat anti-mouse
    detected: (Thermo Fisher Scientific Cat# A32727, RRID:AB_2633276)
    Experimental Models: Cell Lines
    SentencesResources
    SARS-CoV-2 was passaged once in Vero-E6 cells and viral stocks were aliquoted and stored at −80°C.
    Vero-E6
    suggested: None
    Software and Algorithms
    SentencesResources
    Immunofluorescence images were quantified using ImageJ software.
    ImageJ
    suggested: (ImageJ, RRID:SCR_003070)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.04.21.051912v1 on bioRxiv