Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men

Version published to 10.1016/j.stem.2020.11.009

Dec 1, 2020

SciScore for 10.1101/2020.05.12.091082: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

NIH rigor criteria are not applicable to paper type.

Table 2: Resources

Antibodies
Sentences	Resources
24 hours after treatment, cells were fixed stained with ACE2 antibody.	ACE2 suggested: None
The cells were subsequently fixed, permeabilized and stained with antibodies against ACE2 and human Fc receptor.	antibodies against ACE2 suggested: None
Non-specific antigen binding was blocked by treating the cells with PBS+0.5% BSA prior to adding the primary antibodies (ACE2 or TMPRSS2, Proteintech cat#21115-1-AP and Novus Biologicals cat# NBP1-20984, respectively).	TMPRSS2 suggested: (Novus Cat# NBP1-20984, RRID:AB_1643199)
Secondary antibodies and fluorophore conjugated anti human Fc antibody were incubated for 30 …

SciScore for 10.1101/2020.05.12.091082: (What is this?)

Please note, not all rigor criteria are appropriate for all manuscripts.

Table 1: Rigor

NIH rigor criteria are not applicable to paper type.

Table 2: Resources

Antibodies
Sentences	Resources
24 hours after treatment, cells were fixed stained with ACE2 antibody.	ACE2 suggested: None
The cells were subsequently fixed, permeabilized and stained with antibodies against ACE2 and human Fc receptor.	antibodies against ACE2 suggested: None
Non-specific antigen binding was blocked by treating the cells with PBS+0.5% BSA prior to adding the primary antibodies (ACE2 or TMPRSS2, Proteintech cat#21115-1-AP and Novus Biologicals cat# NBP1-20984, respectively).	TMPRSS2 suggested: (Novus Cat# NBP1-20984, RRID:AB_1643199)
Secondary antibodies and fluorophore conjugated anti human Fc antibody were incubated for 30 minutes at room temperature.	anti human Fc suggested: None
Experimental Models: Cell Lines
Sentences	Resources
Transcriptional changes associated with AR down-regulation: We used an existing AR ChIP-seq dataset generated in LNCaP cells to identify direct transcriptional targets of AR.	LNCaP suggested: CLS Cat# 300265/p761_LNCaP, RRID:CVCL_0395)
Software and Algorithms
Sentences	Resources
Protein-protein interaction network construction: Protein-protein interaction network analysis was performed using the Search Tool for the Retrieval of Interacting Genes (STRING) database.	STRING suggested: (STRING, RRID:SCR_005223)
Adobe Illustrator 24.1 was used for visualization.	Adobe Illustrator suggested: (Adobe Illustrator, RRID:SCR_010279)
We downloaded processed peaks from the Gene Expression Omnibus (GSM3148987) and lifted the peaks from hg19 over to hg38.	Gene Expression Omnibus suggested: (Gene Expression Omnibus (GEO, RRID:SCR_005012)
We downloaded raw fastq files and aligned them to the transcriptome (gencode.v28) using Salmon (v0.14.1 using --validateMappings and −l ISR flags).	Salmon suggested: (Salmon, RRID:SCR_017036)
We then used DESeq2 (1.22.2) to compare gene expression changes in response to AR knockdown.	DESeq2 suggested: (DESeq, RRID:SCR_000154)

Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

Results from TrialIdentifier: No clinical trial numbers were referenced.

Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

Results from JetFighter: We did not find any issues relating to colormaps.

Results from rtransparent:

Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
No protocol registration statement was detected.

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Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men

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