Cell Biology

The Hippo kinase cascade regulates a contractile cell behavior and cell density in a close unicellular relative of animals

1. Jonathan E Phillips
2. Duojia Pan

• Curated by eLife

  eLife assessment

  This important study examines the ancestral function of Hippo pathway kinases in contractility and cell density in the ameboid organism Capsaspora owczarzaki, a unicellular animal that is a close relative of multicellular animals. There is convincing evidence for Hippo kinases regulating contractility and cell density but not proliferation in C. owczarzaki. The work complements previous work on the Hippo effector Yorkie homolog in this species, although the unavailability of extensive genetic tools in this species precludes informative epistasis experiments. The work would be of interest to evolutionary and developmental biologists.

Reviewed by eLife

Complementary cytoskeletal feedback loops control signal transduction excitability and cell polarity

1. Jonathan Kuhn
2. Parijat Banerjee
3. Andrew Haye
4. Douglas N. Robinson
5. Pablo A. Iglesias
6. Peter N. Devreotes

Reviewed by preLights

Metabolic adaptation pilots the differentiation of human hematopoietic cells

1. Laëtitia Racine
2. Romuald Parmentier
3. Shreyas Niphadkar
4. Julie Chhun
5. Jean-Alain Martignoles
6. François Delhommeau
7. Sunil Laxman
8. Andras Paldi

Reviewed by Review Commons

Sorting of secretory proteins at the trans-Golgi network by human TGN46

1. Pablo Lujan
2. Carla Garcia-Cabau
3. Yuichi Wakana
4. Javier Vera Lillo
5. Carmen Rodilla-Ramírez
6. Hideaki Sugiura
7. Vivek Malhotra
8. Xavier Salvatella
9. Maria F Garcia-Parajo
10. Felix Campelo

• Curated by eLife

  eLife assessment

  This study provides the fundamental insight that TGN46, a single-pass membrane protein, acts as a cargo receptor for proteins at the Trans-Golgi Network. The authors demonstrate that the luminal domain of TGN46 is crucial for the incorporation of the soluble secretory protein PAUF into CARTS, a class of vesicles mediating TGN to surface traffic. The data presented are compelling, yielding a clear model for the sorting of cargos destined for secretion.

Reviewed by eLife

Binucleated human hepatocytes arise through loss of membrane anchorage to the midbody during endomitosis

1. Gabriella Darmasaputra
2. Susana M. Chuva de Sousa Lopes
3. Hans Clevers
4. Matilde Galli

Reviewed by Review Commons

Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in Drosophila mushroom body contributing to olfactory learning

1. Haibin Yu
2. Dandan Liu
3. Yaowen Zhang
4. Ruijun Tang
5. Xunan Fan
6. Song Mao
7. Lu Lv
8. Fang Chen
9. Hongtao Qin
10. Zhuohua Zhang
11. Daan MF van Aalten
12. Bing Yang
13. Kai Yuan

• Curated by eLife

  eLife assessment

  This work describes a valuable new technique involving proximity labelling to identify Drosophila proteins modified by GlcNAcylation in subsets of cells in vivo. A solid set of experiments shows that several ribosomal proteins are modified in the fly mushroom body. Consistent with a role for GlcNAcylation of ribosomal proteins in control of memory related translational control, the authors show that perturbation of GlcNAc modification in KCs prevents efficient consolidation of long-term memory.

Reviewed by eLife

YAP/TAZ enhances P-body formation to promote tumorigenesis

1. Xia Shen
2. Xiang Peng
3. YueGui Guo
4. Zhujiang Dai
5. Long Cui
6. Wei Yu
7. Yun Liu
8. Chen-Ying Liu

• Curated by eLife

  eLife assessment

  This valuable study advances our understanding that YAP/TAZ, as well as their target genes, play a prominent role in the formation of processing bodies (P-bodies). The evidence supporting the conclusions is convincing. The article could be improved through further analysis to elucidate the mechanistic link between P-body formation and oncogenesis. The work will be of broad interest to scientists working in the field of Hippo signaling and cancer biology.

Reviewed by eLife

WRNIP1 prevents transcription-associated genomic instability

1. Pasquale Valenzisi
2. Veronica Marabitti
3. Pietro Pichierri
4. Annapaola Franchitto

• Curated by eLife

  eLife assessment

  This valuable paper examines the role of the WRNIP1 AAA+ ATPase in regulating R-loop formation, which induces a conflict with active replication forks and transcription. The authors provide convincing evidence to support a role of the ubiquitin-binding UBZ domain of WRNIP1 in R-loop suppression generated by this conflict. The work is of interest to researchers who work on genome stability/instability.

Reviewed by eLife

Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2

1. Ibtisam Ibtisam
2. Alexei F Kisselev

• Curated by eLife

  eLife assessment

  The study presents important findings regarding a transcription-independent component of the early recovery of proteasome activity from a short pulse of proteasome inhibitor treatment, which has not been appreciated before and which is independent of the DDI2-NRF2 axis. While the evidence is in principle solid, with recapitulation in several cell line models, the proposed alternative underlying mechanism, namely regulation at the level of proteasome assembly, lacks experimental support, and at this point remain speculative.

Reviewed by eLife

Universal Cell Embeddings: A Foundation Model for Cell Biology

1. Yanay Rosen
2. Yusuf Roohani
3. Ayush Agrawal
4. Leon Samotorcan
5. Tabula Sapiens Consortium
6. Stephen R. Quake
7. Jure Leskovec

Reviewed by Arcadia Science