Cell Biology

Circadian regulation of macromolecular complex turnover and proteome renewal

1. Estere Seinkmane
2. Anna Edmondson
3. Sew Y Peak-Chew
4. Aiwei Zeng
5. Nina M Rzechorzek
6. Nathan R James
7. James West
8. Jack Munns
9. David CS Wong
10. Andrew D Beale
11. John S O’Neill

Reviewed by Review Commons

Export of discarded splicing intermediates requires mRNA export factors and the nuclear basket

1. Yi Zeng
2. Jonathan P. Staley

• Curated by eLife

  eLife assessment

  This is an important study that demonstrates that RNA intermediates arising from improper splicing are exported out of the nucleus via the canonical mRNA export machinery and the nuclear pore basket. The authors provide convincing evidence that the role of the nuclear basket rather than retaining the transcripts is stimulating their export, putting into question the current model of the role of the basket. The conclusions are in line with recent studies in mammalian cells that suggest that the basket's role in mRNA export and quality control has to be revised.

Reviewed by eLife

Heterotypic interaction promotes asymmetric division of human hematopoietic stem and progenitor cells

1. Adrian Candelas
2. Benoit Vianay
3. Matthieu Gelin
4. Lionel Faivre
5. Jerome Larghero
6. Laurent Blanchoin
7. Manuel Théry
8. Stephane Brunet

Reviewed by Review Commons

A novel role for CSA in the regulation of nuclear envelope integrity: uncovering a non-canonical function

1. Denny Yang
2. Austin Lai
3. Amelie Davies
4. Anne FJ Janssen
5. Matthew O Ellis
6. Delphine Larrieu

Reviewed by Review Commons

Imaging analysis of six human histone H1 variants reveals universal enrichment of H1.2, H1.3, and H1.5 at the nuclear periphery and nucleolar H1X presence

1. Monica Salinas-Pena
2. Elena Rebollo
3. Albert Jordan

• Curated by eLife

  eLife assessment

  This manuscript is an important advance in the study of Histone H1s, finding distinct distributions of various H1 variants in the genome. The controls presented by the authors provide convincing evidence to demonstrate a heterogenous distribution of H1 which might reflect functional regulation of chromatin accessibility by linker histones. This work will be of interest to the genome organization field, and could additionally provide a framework for understanding H1 mis-regulation observed in cancer cells.

Reviewed by eLife

Membrane contact sites regulate vacuolar fission via sphingolipid metabolism

1. Kazuki Hanaoka
2. Kensuke Nishikawa
3. Atsuko Ikeda
4. Philipp Schlarmann
5. Saku Sasaki
6. Sotaro Fujii
7. Sayumi Yamashita
8. Aya Nakaji
9. Kouichi Funato

• Curated by eLife

  eLife assessment

  This manuscript presents valuable findings that contribute to our understanding of how sphingolipids and membrane contact sites, formed by the tethering protein family tricalbins, are involved in regulating vacuolar morphology in S. cerevisiae. The evidence supporting the authors' claims is largely solid. While the reported correlation between sphingolipid levels and vacuole homeostasis is interesting and intriguing, more work is needed to thoroughly substantiate the proposed mechanism. This study will be of interest to cell biologists focusing on intracellular organization and lipid metabolism.

Reviewed by eLife

High-energy demand and nutrient exhaustion in MTCH2 knockout cells

1. Sabita Chourasia
2. Christopher Petucci
3. Hu Wang
4. Xianlin Han
5. Ehud Sivan
6. Alexander Brandis
7. Tevie Mehlman
8. Sergey Malitsky
9. Maxim Itkin
10. Ron Rotkopf
11. Limor Regev
12. Yehudit Zaltsman
13. Atan Gross

Reviewed by Review Commons

The septin cytoskeleton is required for plasma membrane repair

1. M Isabella Prislusky
2. Jonathan G T Lam
3. Viviana Ruiz Contreras
4. Marilynn Ng
5. Madeline Chamberlain
6. Sarika Pathak-Sharma
7. Madalyn Fields
8. Xiaoli Zhang
9. Amal O Amer
10. Stephanie Seveau

Reviewed by Review Commons

Effect of α-tubulin acetylation on the doublet microtubule structure

1. Shun Kai Yang
2. Shintaroh Kubo
3. Corbin Steven Black
4. Katya Peri
5. Daniel Dai
6. Thibault Legal
7. Melissa Valente-Paterno
8. Jacek Gaertig
9. Khanh Huy Bui

• Curated by eLife

  eLife assessment

  This fundamental study employs a combination of cryo-electron microscopy, molecular dynamics, and mass spectrometry to elucidate the role of α-tubulin acetylation at the lumenal lysine 40 residue (αK40) within the cilium. Compelling evidence shows αK40 acetylation to impact the structure and stability of doublet microtubules in cilia by affecting the lateral rotational angle. The work will be of relevance to those interested in cytoskeleton and structural biology.

Reviewed by eLife

Novel autophagy inducers by accelerating lysosomal clustering against Parkinson’s disease

1. Yuki Date
2. Yukiko Sasazawa
3. Mitsuhiro Kitagawa
4. Kentaro Gejima
5. Ayami Suzuki
6. Hideyuki Saya
7. Yasuyuki Kida
8. Masaya Imoto
9. Eisuke Itakura
10. Nobutaka Hattori
11. Shinji Saiki

Reviewed by Review Commons