Cell Biology

Plasmodium RON11 triggers biogenesis of the merozoite rhoptry pair and is essential for erythrocyte invasion

1. David Anaguano
2. Opeoluwa Adewale-Fasoro
3. Grace W. Vick
4. Sean Yanik
5. James Blauwkamp
6. Manuel A. Fierro
7. Sabrina Absalon
8. Prakash Srinivasan
9. Vasant Muralidharan

Reviewed by preLights

Tgif1-deficiency impairs cytoskeletal architecture in osteoblasts by activating PAK3 signaling

1. Simona Bolamperti
2. Hiroaki Saito
3. Sarah Heerdmann
4. Eric Hesse
5. Hanna Taipaleenmäki

• Curated by eLife

  eLife assessment

  This important work substantially advances our understanding of osteoblast migration to the sites of bone formation and regeneration. The evidence supporting the conclusion is compelling, with rigorous in vitro assays for cellular and biochemical aspects and with appropriate in vivo models. The work will be of broad interest to developmental biologists and bone biologists.

Reviewed by eLife

Pigmentation level of human iPSC-derived RPE does not indicate a specific gene expression profile

1. Yoko Nakai-Futatsugi
2. Jianshi Jin
3. Taisaku Ogawa
4. Noriko Sakai
5. Akiko Maeda
6. Ken-ichi Hironaka
7. Masakazu Fukuda
8. Hiroki Danno
9. Yuji Tanaka
10. Seiji Hori
11. Katsuyuki Shiroguchi
12. Masayo Takahashi

• Curated by eLife

  eLife assessment

  This useful work describes a novel microscopy-based method to correlate the degree of pigmentation with the gene expression profile of human-induced pluripotent stem cell-derived Retinal Pigmented Epithelial (iPSC-RPE) cells at the single cell level. The presented evidence is solid in showing that there is heterogeneous gene expression in iPSC-derived RPE cells, and there is no significant correlation with the pigmentation. By analyzing the expression of some genes related to function, lysosomal- and complement-related pathways were partially enriched in darker cells. This methodology can be used by other researchers interested in analyzing gene expression related to microscopic images.

Reviewed by eLife

The doublecortin-family kinase ZYG-8 ^DCLK1 regulates motor activity to achieve proper force balance in C. elegans acentrosomal spindles

1. Emily R. Czajkowski
2. Nikita S. Divekar
3. Sarah M. Wignall

Reviewed by Review Commons

FAM210A mediates an inter-organelle crosstalk essential for protein synthesis and muscle growth in mouse

1. Jingjuan Chen
2. Feng Yue
3. Kun Ho Kim
4. Peipei Zhu
5. Jiamin Qiu
6. W. Andy Tao
7. Shihuan Kuang

Reviewed by Review Commons

Pathogenic Huntingtin aggregates alter actin organization and cellular stiffness resulting in stalled clathrin-mediated endocytosis

1. Surya Bansi Singh
2. Shatruhan Singh Rajput
3. Aditya Sharma
4. Sujal Kataria
5. Priyanka Dutta
6. Vaishnavi Ananthanarayanan
7. Amitabha Nandi
8. Shivprasad Patil
9. Amitabha Majumdar
10. Deepa Subramanyam

Reviewed by Review Commons, Arcadia Science

Chemotherapy activates inflammasomes to cause inflammation-associated bone loss

1. Chun Wang
2. Khushpreet Kaur
3. Canxin Xu
4. Yousef Abu-Amer
5. Gabriel Mbalaviele

• Curated by eLife

  eLife assessment

  This useful study, which systematically addresses off-target effects of a commonly used chemotherapy drug on bone and bone marrow cells and which therefore is of potential interest to a broad readership, presents evidence that reducing systemic inflammation induced by doxorubicin limits bone loss to some extent. The demonstration of the effect of systemic inflammation on bone loss is convincing. Building on prior work, this study sets the scene for additional genetic and pharmacologic experiments as well as future analyses of the bone phenotypes, which should speak to the mechanisms involved in doxorubicin-induced bone loss – which are not addressed in the current study – and which may substantiate the clinical relevance of targeting inflammation in order to limit the negative impact of chemotherapies on bone quality.

Reviewed by eLife

DHCR24-mediated sterol homeostasis during spermatogenesis is required for sperm mitochondrial sheath formation and impacts male fertility over time

1. Sona Relovska
2. Huafeng Wang
3. Xinbo Zhang
4. Pablo Fernández-Tussy
5. Kyung Jo Jeong
6. Jungmin Choi
7. Yajaira Suárez
8. Jeffrey G. McDonald
9. Carlos Fernández-Hernando
10. Jean-Ju Chung

• Curated by eLife

  eLife assessment

  This useful study reports data supporting the importance of sterol homeostasis in sperm development and consequently male reproduction. While most of the data are supportive of the conclusion, some remain incomplete and need more experimental verification. This work would be of interest to basic researchers and clinicians working on sterol homeostasis and male fertility.

Reviewed by eLife

Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies

1. Mathieu C Husser
2. Nhat P Pham
3. Chris Law
4. Flavia RB Araujo
5. Vincent JJ Martin
6. Alisa Piekny

• Curated by eLife

  eLife assessment

  In this study, the authors develop a strategy for fluorophore-tagging endogenous proteins in human induced pluripotent stem cells (iPSCs) using a split mNeonGreen approach, and they conclude that the system will be appropriate for performing live imaging studies of highly dynamic cellular processes such as cytokinesis in iPSCs. Experimentally, the methods are solid, and the data presented support the authors' conclusions. Overall, these methodologies should be useful to a wide audience of cell biologists who want to study protein localization and dynamics at endogenous levels in iPSCs.

Reviewed by eLife

Cytoplasmic protein-free mRNA induces stress granules by two G3BP1/2-dependent mechanisms

1. Sean J. Ihn
2. Laura Farlam-Williams
3. Alexander F. Palazzo
4. Hyun O. Lee

Reviewed by PREreview