Cell Biology

Loss of Ptpmt1 limits mitochondrial utilization of carbohydrates and leads to muscle atrophy and heart failure in tissue-specific knockout mice

1. Hong Zheng
2. Qianjin Li
3. Shanhu Li
4. Zhiguo Li
5. Marco Brotto
6. Daiana Weiss
7. Domenick Prosdocimo
8. Chunhui Xu
9. Ashruth Reddy
10. Michelle Puchowicz
11. Xinyang Zhao
12. M Neale Weitzmann
13. Mukesh K Jain
14. Cheng-Kui Qu

• Curated by eLife

  eLife assessment

  This paper provides a useful set of data examining the role of PTPMT1, a mitochondria-based phosphatase, in mitochondrial fuel selection. The data were collected and analyzed using solid methodology and can be used as a starting point for further studies that build on the findings here.

Reviewed by eLife

Interaction between Teneurin-2 and microtubules via EB proteins provides a platform for GABAA receptor exocytosis

1. Sotaro Ichinose
2. Yoshihiro Susuki
3. Nobutake Hosoi
4. Ryosuke Kaneko
5. Mizuho Ebihara
6. Hirokazu Hirai
7. Hirohide Iwasaki

• Curated by eLife

  eLife assessment

  This potentially important paper investigates the mechanisms that contribute to building inhibitory synapses through differential protein release from microtubules. The experiments are generally designed well, but the evidence supporting the conclusions is incomplete. This manuscript will be of interest to neuroscientists and cell biologists interested in intracellular trafficking and synapse maturation.

Reviewed by eLife

FAM76B regulates NF-κB-mediated inflammatory pathway by influencing the translocation of hnRNPA2B1

1. Dongyang Wang
2. Xiaojing Zheng
3. Lihong Chai
4. Junli Zhao
5. Jiuling Zhu
6. Yanqing Li
7. Peiyan Yang
8. Qinwen Mao
9. Haibin Xia

• Curated by eLife

  eLife assessment

  The paper provides important insight into the function of FAM76B protein as a regulator of inflammation. The knockout/overexpression data are solid, however, the mechanism of regulation and the role of FAM76B in neurodegeneration is incomplete and requires additional experimentation. The work will be of interest to researchers studying inflammation, particularly neuroinflammation.

Reviewed by eLife

An extracellular vesicle targeting ligand that binds to Arc proteins and facilitates Arc transport in vivo

1. Peter H Lee
2. Michael Anaya
3. Mark S Ladinsky
4. Justin M Reitsma
5. Kai Zinn

• Curated by eLife

  eLife assessment

  Extracellular vesicles (EVs) are emerging as important mediators of cell-to-cell signaling. Lee et al show convincingly that Stranded at second (Sas), a Drosophila cell surface protein, is trafficked by and localized to Drosophila EVs. However, the data supporting interaction with dArc and whether Sas facilitates the intercellular transfer of dArc protein or mRNA is incomplete. Moreover, almost all experiments rely on gain-of-function and over-expression of Sas, thus the relevance to normal physiological signaling is unclear.

Reviewed by eLife

Histone deacetylase 6 inhibition promotes microtubule acetylation and facilitates autophagosome-lysosome fusion in dystrophin-deficient mdx mice

1. Akanksha Agrawal
2. Erin L. Clayton
3. Courtney L. Cavazos
4. Benjamin A. Clayton
5. George G. Rodney

• Curated by eLife

  eLife assessment

  This important study pinpoints nitrite oxide synthase 2 activity and decreased microtubule acetylation as distinct regulators of altered autophagic flux that may contribute to pathogenesis in a mouse model of Duchenne muscular dystrophy. While most of the evidence to support these claims is convincing, the claim that autophagy is improved with increased microtubule acetylation is incompletely supported. This work may be of broad interest to muscle biologists and has translational potential for the treatment of Duchenne muscular dystrophy.

Reviewed by eLife

TANGO-Light - optogenetic control of transcriptional modulators

1. Alicja Przybyszewska-Podstawka
2. Joanna Kałafut
3. Jakub Czapiński
4. Thu Ha Ngo
5. Arkadiusz Czerwonka
6. Adolfo Rivero-Müller

Reviewed by Arcadia Science

Syntenin and CD63 Promote Exosome Biogenesis from the Plasma Membrane by Blocking Cargo Endocytosis

1. Yiwei Ai
2. Chenxu Guo
3. Marta Garcia-Contreras
4. Laura S. Sánchez B.
5. Andras Saftics
6. Oluwapelumi Shodubi
7. Shankar Raghunandan
8. Junhao Xu
9. Shang Jui Tsai
10. Yi Dong
11. Rong Li
12. Tijana Jovanovic-Talisman
13. Stephen J. Gould

Reviewed by Arcadia Science

Arp2/3 Complex Activity Enables Nuclear YAP for Naïve Pluripotency of Human Embryonic Stem Cells

1. Nathaniel P. Meyer
2. Tania Singh
3. Matthew L. Kutys
4. Todd Nystul
5. Diane L. Barber

Reviewed by Review Commons

The yeast endocytic early/sorting compartment exists as an independent sub-compartment within the trans-Golgi network

1. Junko Y Toshima
2. Ayana Tsukahara
3. Makoto Nagano
4. Takuro Tojima
5. Daria E Siekhaus
6. Akihiko Nakano
7. Jiro Toshima

• Curated by eLife

  eLife assessment

  In this study, the authors use high-speed and high-resolution imaging to investigate the role of the yeast syntaxin homolog Tlg2p in endocytic vesicle sorting. They obtain compelling data to show that the Tlg2p-residing compartment within the trans-Golgi network functions as an early/sorting compartment, where endocytic cargos are sorted to either the recycling pathway or the endo-lysosomal pathway. The authors also describe additional molecular details of this sorting process, and overall provide important insights into the mechanism of endocytic vesicle sorting in budding yeast.

Reviewed by eLife

Phosphorylation of tyrosine 90 in SH3 domain is a new regulatory switch controlling Src kinase

1. Lenka Koudelková
2. Markéta Pelantová
3. Zuzana Brůhová
4. Martin Sztacho
5. Vojtěch Pavlík
6. Dalibor Pánek
7. Jakub Gemperle
8. Pavel Talacko
9. Jan Brábek
10. Daniel Rösel

• Curated by eLife

  eLife assessment

  This manuscript explores the potential regulatory role of a previously unstudied phosphorylation site in the Src kinase SH3 domain. A mutant intended to mimic the phosphorylation of this site, Y90E, shows enhanced activity and transforming capacity, reduced mobility in the lipid bilayer, and a more open catalytic structure. In general, these findings are supported by compelling evidence. The paper will be of interest to biochemists and structural biologists studying new mechanisms that are capable of modulating the allosteric regulation of multi-domain protein kinases.

Reviewed by eLife