Cell Biology

Cardiac glycosides restore autophagy flux in an iPSC-derived neuronal model of WDR45 deficiency

1. Apostolos Papandreou
2. Nivedita Singh
3. Lorita Gianfrancesco
4. Dimitri Budinger
5. Katy Barwick
6. Alexander Agrotis
7. Christin Luft
8. Ying Shao
9. An-Sofie Lenaerts
10. Allison Gregory
11. Suh Young Jeong
12. Penelope Hogarth
13. Susan Hayflick
14. Serena Barral
15. Janos Kriston-Vizi
16. Paul Gissen
17. Manju A Kurian
18. Robin Ketteler

• Curated by eLife

  eLife assessment

  This valuable manuscript reports alterations in autophagy present in dopaminergic neurons differentiated from iPSCs of patients with WDR45 mutations. The authors identified compounds that improved the defects present in mutant cells by generating isogenic iPSC without the mutation and performing an automated drug screening. The methodological approaches are solid, but the claims still need to be completed; showing the effects of the identified compounds on iron-related alterations is crucial. The effects of these drugs in vivo would be a great addition to the study.

Reviewed by eLife

Reactive oxygen species suppress phagocyte surveillance by oxidizing cytoskeletal regulators

1. Iuliia Ferling
2. Steffen Pfalzgraf
3. Lea Moutounet
4. Lanhui Qiu
5. Yumeng Li
6. Yuhuan Zhou
7. Sergio Grinstein
8. Spencer A Freeman

• Curated by eLife

  eLife assessment

  In this study, Ferling and colleagues provide convincing evidence demonstrating that myeloid cells exert distinct, cargo-dependent responses during and after phagocytosis. These important findings establish previously unrecognized insights into the function(s) of myeloid cells in immunosurveillance and are thus likely to be broadly impactful across the spectrum of biomedical disciplines including immunology and cell biology. Notwithstanding these clear strengths of the article, some minor issues were noted pertinent to the relative opaqueness of the mechanisms underpinning context-specific RhoA activation.

Reviewed by eLife

Multi-tissue proteomics identifies a link between satellite DNA organization and heritable transposon repression in Drosophila

1. Ankita Chavan
2. Lena Skrutl
3. Federico Uliana
4. Melanie Pfister
5. Franziska Brändle
6. Laszlo Tirian
7. Delora Baptista
8. Dominik Handler
9. David Burke
10. Anna Sintsova
11. Pedro Beltrao
12. Julius Brennecke
13. Madhav Jagannathan

Reviewed by Review Commons

APEX-based proximity labeling in Plasmodium identifies a membrane protein with dual functions during mosquito infection

1. Jessica Kehrer
2. Dominik Ricken
3. Leanne Strauss
4. Emma Pietsch
5. Julia M. Heinze
6. Friedrich Frischknecht

Reviewed by Review Commons

The role of ER exit sites in maintaining P-body organization and transmitting ER stress response during Drosophila melanogaster oogenesis

1. Samantha N. Milano
2. Livia V. Bayer
3. Julie J. Ko
4. Caroline E. Casella
5. Diana P. Bratu

Reviewed by preLights

Significantly reduced, but balanced, rates of mitochondrial fission and fusion are sufficient to maintain the integrity of yeast mitochondrial DNA

1. Brett T. Wisniewski
2. Jason C. Casler
3. Laura L. Lackner

Reviewed by preLights