Cell Biology

Novel Nuclear Roles for Testis-Specific ACTL7A and ACTL7B Supported by In Vivo Characterizations and AI Facilitated In Silico Mechanistic Modeling with Implications for Epigenetic Regulation in Spermiogenesis

1. Pierre Ferrer
2. Srijana Upadhyay
3. James J. Cai
4. Tracy M. Clement

• Curated by eLife

  eLife assessment

  This valuable study reports that actin-related proteins may be involved in transcriptional regulation during spermatogenesis. The supporting data remain incomplete, and more extensive disentanglement from the canonical role of these actin-related proteins and the experimental validation of in silico predictions are required. This work will be of interest to reproductive biologists and other researchers working on non-canonical roles of actin and actin-related proteins.

Reviewed by eLife

Temporally controlled nervous system-to-gut signaling bidirectionally regulates longevity in C. elegans

1. Lingxiu Xu
2. Chengxuan Han
3. Lei Chun
4. X.Z. Shawn Xu
5. Jianfeng Liu

• Curated by eLife

  eLife assessment

  This study reports that the timing of 'brain-to-gut' signaling influences the lifespan of the C. elegans model. The main finding, that modulating the same neurotransmitter, Acetylcholine, at different ages elicits lifespan shortening - or extending - effects utilizing different receptors, is important and of broad interest to the longevity field as recognized by all the reviewers. The data is largely consistent with the authors' model, but the strength of the evidence is incomplete. The study requires several rigorous experiments detailed by the reviewers to substantiate the main conclusions.

Reviewed by eLife

O-GlcNAcylation of the intellectual disability protein DDX3X exerts proteostatic cell cycle control

1. Conor W. Mitchell
2. Huijie Yuan
3. Marie Sønderstrup-Jensen
4. Andrew T. Ferenbach
5. Daan M. F. van Aalten

Reviewed by Review Commons

Mir221/222 drive synovial hyperplasia and arthritis by targeting cell cycle inhibitors and chromatin remodeling components

1. Fani Roumelioti
2. Christos Tzaferis
3. Dimitris Konstantopoulos
4. Dimitra Papadopoulou
5. Alejandro Prados
6. Maria Sakkou
7. Anastasios Liakos
8. Panagiotis Chouvardas
9. Theodore Meletakos
10. Yiannis Pandis
11. Niki Karagianni
12. Maria C Denis
13. Maria Fousteri
14. Maria Armaka
15. George Kollias

• Curated by eLife

  eLife assessment

  This important manuscript used state-of-the-art techniques and employed relevant animal models to provide both convincing and solid evidence supporting the regulatory role of microRNA cluster 221/222 in rheumatoid arthritis synovial fibroblast. The findings of this work offer significant advances to current knowledge which will be interesting to a wide range audience in the rheumatology and bone research fields. However, whereas models, techniques, and analyses are solid, certain concepts related to the role of immune and bone cells are limited.

Reviewed by eLife

Nucleocytoplasmic transport senses mechanics independently of cell density in cell monolayers

1. Ignasi Granero-Moya
2. Guillaume Belthier
3. Bart Groenen
4. Marc Molina-Jordán
5. Miguel González-Martín
6. Xavier Trepat
7. Jacco van Rheenen
8. Ion Andreu
9. Pere Roca-Cusachs

Reviewed by Review Commons

G6b-B antibody-based cis-acting platelet receptor inhibitors (CAPRIs) as a new family of anti-thrombotic therapeutics

1. Alexandra Mazharian
2. Ophélie Bertin
3. Amrita Sarkar
4. Johanna Augros
5. Alicia Bornert
6. Cécile Loubière
7. Friederike Jönsson
8. Juli Warwicker
9. W Mark Abbott
10. Omer Dushek
11. Caroline Vayne
12. Lubica Rauova
13. Klaus Fütterer
14. Jérôme Rollin
15. Mortimer Poncz
16. Yotis A Senis

Reviewed by preLights

Alstrom syndrome proteins regulate centriolar cartwheel assembly by enabling Plk4-Ana2 amplification loop in Drosophila

1. Marine Brunet
2. Joelle Thomas
3. Jean-Andre Lapart
4. Léo Krüttli
5. Marine Laporte
6. Maria Giovanna Riparbelli
7. Giuliano Callaini
8. Benedicte Durand
9. Veronique Morel

Reviewed by Review Commons

PILS-Nir1 is a novel phosphatidic acid biosensor that reveals mechanisms of lipid production

1. Claire C. Weckerly
2. Taylor A. Rahn
3. Max Ehrlich
4. Rachel C. Wills
5. Joshua G. Pemberton
6. Michael V. Airola
7. Gerald R. V. Hammond

Reviewed by Review Commons

Vacuolar H+-ATPase determines daughter cell fates through asymmetric segregation of the nucleosome remodeling and deacetylase complex

1. Zhongyun Xie
2. Yongping Chai
3. Zhiwen Zhu
4. Zijie Shen
5. Zhengyang Guo
6. Zhiguang Zhao
7. Long Xiao
8. Zhuo Du
9. Guangshuo Ou
10. Wei Li

• Curated by eLife

  eLife assessment

  The authors make the intriguing proposal that the NuRD complex in C. elegans, which has been linked to regulation of the cell death protein EGL-1 before, becomes asymmetrically distributed after cell division and that this asymmetry relies on V-ATPase activity. Whereas some disagreement remained between the reviewers' and the authors' interpretation, the final version incorporated alternative possibilities in the text, and with careful interpretation, the current manuscript's model is supported by solid data, and represents a valuable contribution to the field.

Reviewed by eLife

Growth-dependent concentration gradient of the oscillating Min system in Escherichia coli

1. Claudia Parada
2. Ching-Cher Sanders Yan
3. Cheng-Yu Hung
4. I-Ping Tu
5. Chao-Ping Hsu
6. Yu-Ling Shih

Reviewed by Review Commons