Cell Biology

Time-resolved interactome profiling deconvolutes secretory protein quality control dynamics

1. Madison T Wright
2. Bibek Timalsina
3. Valeria Garcia Lopez
4. Jake N Hermanson
5. Sarah Garcia
6. Lars Plate

Reviewed by Review Commons

Differential regulation by CD47 and thrombospondin-1 of extramedullary erythropoiesis in mouse spleen

1. Rajdeep Banerjee
2. Thomas J Meyer
3. Margaret C Cam
4. Sukhbir Kaur
5. David D Roberts

• Curated by eLife

  eLife assessment

  This study presents a valuable finding on the cell composition in mouse spleen depleted for the CD47 receptor and its signaling ligand Thrombospondin in hematopoietic differentiation. The supporting evidence is convincing with analytical improvements on the individual contributions of the signaling components and with functional studies. This work has implications for the role of CD47/Thsp in extramedullary erythropoiesis in mouse spleen and will be of interest to medical biologists working on cell signaling, transfusion medicine, and cell therapy.

Reviewed by eLife

Membrane lipid poly-unsaturation selectively affects ligand induced dopamine D2 receptor internalization

1. Silvia Sposini
2. Rim Baccouch
3. Mathias Lescuyer
4. Véronique De Smedt-Peyrusse
5. Joyce Heuninck
6. Thierry Durroux
7. Pierre Trifilieff
8. David Perrais
9. Isabel Alves

Reviewed by Review Commons

Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects

1. Ritobrata Ghose
2. Fabio Pezzano
3. Rémi Badia
4. Savvas Kourtis
5. Ilir Sheraj
6. Shubhamay Das
7. Antoni Gañez Zapater
8. Upamanyu Ghose
9. Sara Musa-Afaneh
10. Lorena Espinar
11. Albert Coll-Manzano
12. Katja Parapatics
13. Saška Ivanova
14. Paula Sànchez-Fernàndez-de-Landa
15. Dragana Radivojevikj
16. Valeria Venturini
17. Stefan Wieser
18. Antonio Zorzano
19. André C. Müller
20. Verena Ruprecht
21. Sara Sdelci

Reviewed by preLights

Neutrophils actively swell to potentiate rapid migration

1. Tamas L Nagy
2. Evelyn Strickland
3. Orion D Weiner

• Curated by eLife

  eLife assessment

  This fundamental study significantly advances our understanding of the role of water influx and swelling on neutrophil migration in response to chemoattractant. The evidence supporting the conclusions, based on a genome-wide CRISPR screen and high quality cellular observations, is compelling. This paper will be of interest to cell biologists and biophysicists working on cell migration.

Reviewed by eLife

Dietary bacteria control C. elegans fat content through pathways converging at phosphatidylcholine

1. Hsiao-Fen Han
2. Shao-Fu Nien
3. Hang-Shiang Jiang
4. Jui-Ching Wu
5. Chia-Yi Chiang
6. Man-Tzu Li
7. Leng-Jie Huang
8. Sufeng Chiang
9. Lien-Chieh Lin
10. Yi-Ting Chuang
11. Yu-Ho Lin
12. Chao-Wen Wang
13. Yi-Chun Wu

• Curated by eLife

  eLife assessment

  This a useful study that reports a genetic regulatory network that accounts for altered lipid metabolism in response to two different bacterial diets of C. elegans. The proposed mechanism, linking vitamin B12, S-adenosyl methionine (SAM), phosphatidylcholine (PC), and neutral lipid levels, is solid but has been previously demonstrated by other studies using similar assays. The evidence to support a new layer of regulation, via the production of phospho-choline by ASM-3/acid sphingomyelinase, requires further substantiation.

Reviewed by eLife

Coordination between cytoskeletal organization, cell contraction and extracellular matrix development, is depended on LOX for aneurysm prevention

1. Rohtem Aviram
2. Shelly Zaffryar-Eilot
3. Anna Kaganovsky
4. Anas Odeh
5. Shay Melamed
6. Ruslana Militsin
7. Cameron B. Pinnock
8. Ariel Shemesh
9. Raz Palty
10. Santhi K. Ganesh
11. Peleg Hasson

Reviewed by Review Commons

Identification of a third myosin-5a-melanophilin interaction that mediates the association of myosin-5a with melanosomes

1. Jiabin Pan
2. Rui Zhou
3. Lin-Lin Yao
4. Jie Zhang
5. Ning Zhang
6. Qing-Juan Cao
7. Shaopeng Sun
8. Xiang-dong Li

• Curated by eLife

  eLife assessment

  This study represents a useful description of a third interaction site between melanophilin and myosin-5a which has a role in regulating the distribution of pigment granules in melanocytes. While much of the data forms a solid case for this interaction, the inclusion of controls for the cellular studies and measurement of interaction affinities would have been helpful.

Reviewed by eLife

Re-assessment of the subcellular localization of Bazooka/Par-3 in Drosophila : No evidence for localization to the nucleus and the neuromuscular junction

1. Soya Kim
2. Jaffer Shahab
3. Andreas Wodarz

Reviewed by Review Commons

The non-mitotic role of HMMR in regulating the localization of TPX2 and the dynamics of microtubules in neurons

1. Yi-Ju Chen
2. Shun-Cheng Tseng
3. Peng-Tzu Chen
4. Eric Hwang

• Curated by eLife

  eLife assessment

  In their valuable study, Chen et al. investigate the neuronal role of HMMR, a microtubule-associated protein typically associated with cell division. Their findings indicate that HMMR is necessary for proper neuronal morphology and the generation of polymerizing microtubules within neurites, potentially by promoting the function of TPX2. This solid body of work is the first step in deciphering the influence of a mitotic microtubule-associated protein in organizing microtubules in neurons and will be of interest to the neurobiology and cytoskeleton fields.

Reviewed by eLife