Cell Biology

Deep3DSIM: Super-resolution imaging of thick tissue using 3D structured illumination with adaptive optics

1. Jingyu Wang
2. Danail Stoychev
3. Mick A Phillips
4. David Miguel Susano Pinto
5. Richard M Parton
6. Nicholas James Hall
7. Joshua S Titlow
8. Ana Rita Faria
9. Matthew Wincott
10. Dalia Gala
11. Andreas Gerondopoulos
12. Niloufer Irani
13. Ian Dobbie
14. Lothar Schermelleh
15. Martin J Booth
16. Ilan Davis

Reviewed by Review Commons

Damage-induced basal epithelial cell migration modulates the spatial organization of redox signaling and sensory neuron regeneration

1. Alexandra M Fister
2. Adam Horn
3. Michael R Lasarev
4. Anna Huttenlocher

• Curated by eLife

  eLife assessment

  This important study identifies a novel link between the early keratinocyte response to wounds and the subsequent regenerative capacity of local sensory neurons. The evidence supporting the claims of the authors is convincing, although inclusion of conditional genetics or cell-autonomy tests would have strengthened the mechanistic aspects. The work will be of interest to cell and developmental biologists interested in tissue regeneration and cell interactions in a broader context.

Reviewed by eLife

Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia

1. Yoshinobu Ichimura
2. Yuki Sugiura
3. Yoshinori Katsuragi
4. Yu-Shin Sou
5. Takefumi Uemura
6. Naoki Tamura
7. Satoko Komatsu-Hirota
8. Takashi Ueno
9. Masato Koike
10. Satoshi Waguri
11. Masaaki Komatsu

• Curated by eLife

  eLife assessment

  This paper presents useful findings on the dysmyelination phenotype of nervous system-specific Spns1 (a lysosomal lipid transporter) knockout mice. While the analysis of the phenotype is solid, the evidence for the underlying mechanisms, especially the molecular function for SPNS1, is incomplete. With more careful interpretation and/or additional experimental data, this work could have implications for understanding lipid transport and lysosomal storage diseases.

Reviewed by eLife

The ubiquitin-conjugating enzyme UBE2D/eff maintains a youthful proteome and ensures protein quality control during aging

1. Liam C Hunt
2. Kudzai Nyamkondiwa
3. Anna Stephan
4. Jianqin Jiao
5. Kanisha Kavdia
6. Vishwajeeth Pagala
7. Junmin Peng
8. Fabio Demontis

• Curated by eLife

  eLife assessment

  This valuable study presents findings on the role of the ubiquitin-conjugating enzyme UBE2D/eff in maintaining proteostasis during aging. The evidence supporting the conclusions is solid, although one reviewer had concerns about the readout for protein aggregation and the loss-of-function studies. In the future, mechanistic insights explaining the impact of UBE2D/eff deficiency on the accumulation of poly-ubiquitinated proteins and in shortening lifespan would be interesting. The present study is of broad interest to cell biologists working in aging and age-related diseases.

Reviewed by eLife

Compartmentalized Cytoplasmic Flows Direct Protein Transport to the Cell’s Leading Edge

1. Catherine G. Galbraith
2. Brian P. English
3. Ulrike Boehm
4. James A. Galbraith

Reviewed by Arcadia Science

Phosphorylation of Aly3 C-terminus impedes aberrant endocytosis of S. pombe hexose transporter Ght5

1. Yusuke Toyoda
2. Fumie Masuda
3. Shigeaki Saitoh

Reviewed by Review Commons

TPR is required for cytoplasmic chromatin fragment formation during senescence

1. Bethany M Bartlett
2. Yatendra Kumar
3. Shelagh Boyle
4. Tamoghna Chowdhury
5. Andrea Quintanilla
6. Charlene Boumendil
7. Juan Carlos Acosta
8. Wendy A Bickmore

Reviewed by Review Commons

Editing of endogenous tubulins reveals varying effects of tubulin posttranslational modifications on axonal growth and regeneration

1. Yu-Ming Lu
2. Shan Yan
3. Shih-Chieh Ti
4. Chaogu Zheng

• Curated by eLife

  eLife assessment

  This fundamental study analyzes the roles of post-translational modifications of tubulin by generating a large panel of tubulin mutants and describing their effects on morphogenesis and function of sensory neurons in C. elegans. The work, which is of interest to all cell biologists, in particular researchers with an interest in the microtubule cytoskeleton and neurobiology, presents conclusions that are supported by solid evidence. Demonstrating that all introduced mutations have the intended consequences and exploring their direct effect on microtubules would further increase the impact of the work.

Reviewed by eLife

Heparan sulfate-dependent phase separation of CCL5 and its chemotactic activity

1. Xiaolin Yu
2. Guangfei Duan
3. Pengfei Pei
4. Long Chen
5. Renji Gu
6. Wenrui Hu
7. Hongli Zhang
8. Yan-Dong Wang
9. Lili Gong
10. Lihong Liu
11. Ting-Ting Chu
12. Jin-Ping Li
13. Shi-Zhong Luo

• Curated by eLife

  eLife assessment

  How the triplicate interaction between chemokines with both GAGs and G protein-coupled receptors (GPCR) works and how gradients are created and potentially maintained in vivo are poorly understood. The authors provide solid evidence to show phase separation can drive chemotactic gradient formation. The paper is a useful advance in the field of chemokine biology.

Reviewed by eLife

The endoplasmic reticulum stress sensor IRE1 regulates collagen secretion through the enforcement of the proteostasis factor P4HB/PDIA1 contributing to liver damage and fibrosis

1. Younis Hazari
2. Hery Urra
3. Valeria A. Garcia Lopez
4. Javier Diaz
5. Giovanni Tamburini
6. Mateus Milani
7. Philippe Pihan
8. Sylvere Durand
9. Fanny Aprahamia
10. Reese Baxter
11. Menghao Huang
12. X Charlie Dong
13. Helena Vihinen
14. Ana Batista-Gonzalez
15. Patricio Godoy
16. Alfredo Criollo
17. Vlad Ratziu
18. Fabienne Foufelle
19. Jan G. Hengstler
20. Eija Jokitalo
21. Beatrice Bailly-maitre
22. Jessica L Maiers
23. Lars Plate
24. Guido Kroemer
25. Claudio Hetz

Reviewed by Review Commons