Cell Biology

Actin dynamics sustains spatial gradients of membrane tension in adherent cells

1. Juan Manuel García-Arcos
2. Amine Mehidi
3. Julissa Sánchez Velázquez
4. Pau Guillamat
5. Caterina Tomba
6. Laura Houzet
7. Laura Capolupo
8. Giovanni D’Angelo
9. Adai Colom
10. Elizabeth Hinde
11. Charlotte Aumeier
12. Aurélien Roux

Reviewed by preLights

Proteomic landscape of tunneling nanotubes reveals CD9 and CD81 tetraspanins as key regulators

1. Roberto Notario Manzano
2. Thibault Chaze
3. Eric Rubinstein
4. Esthel Penard
5. Mariette Matondo
6. Chiara Zurzolo
7. Christel Brou

• Curated by eLife

  eLife assessment

  Notario Manzano et al. offer a valuable first analysis of proteins within tunneling nanotubes (TNTs), membranous bridges connecting cells. This work distinguishes TNTs from extracellular vesicles, but further experimental and analytical tools are needed to refine the TNT proteome. Solid data supports a role for tetraspanins CD9 and CD81 in TNT function. The proposed model for CD9 and CD81 is over-interpreted and requires additional evidence for stronger support.

Reviewed by eLife

Ca ²⁺ binding to Esyt is required to modulate membrane contact site density in Drosophila photoreceptors

1. Vaisaly R Nath
2. Harini Krishnan
3. Shirish Mishra
4. Padinjat Raghu

Reviewed by Review Commons

Identification of Glucosidase II that regulates STIM1 activation dynamics

1. Yangchun Du
2. Sisi Zheng
3. Feifan Wang
4. Jia Li
5. Xiaoman Yuan
6. Chao Xi
7. Yandong Zhou
8. Youjun Wang

Reviewed by PREreview

The ORP9-ORP11 dimer promotes sphingomyelin synthesis

1. Birol Cabukusta
2. Shalom Borst Pauwels
3. Jimmy JLL Akkermans
4. Niek Blomberg
5. Aat A Mulder
6. Roman I Koning
7. Martin Giera
8. Jacques Neefjes

• Curated by eLife

  eLife assessment

  This valuable manuscript systematically addresses the role of intracellular lipid transfer proteins on cellular lipid levels. It provides convincing evidence on the role of ORP9 and ORP11 in sphingolipid metabolism at the Golgi complex. This article will be of broad interest to cell biologists interested in lipid metabolism and membrane biology.

Reviewed by eLife

Spatial proteomics of ER tubules reveals CLMN, an ER-actin tether at focal adhesions that promotes cell migration

1. Holly Merta
2. Kaitlynn Gov
3. Tadamoto Isogai
4. Blessy Paul
5. Achinta Sannigrahi
6. Arun Radhakrishnan
7. Gaudenz Danuser
8. W. Mike Henne

Reviewed by PREreview

Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy

1. Mitsuhiro Abe
2. Masataka Yanagawa
3. Michio Hiroshima
4. Toshihide Kobayashi
5. Yasushi Sako

Reviewed by Review Commons

Activation of the mitochondrial unfolded protein response regulates the formation of stress granules

1. Marta Lopez-Nieto
2. Zhaozhi Sun
3. Emily Relton
4. Rahme Safakli
5. Brian D. Freibaum
6. J Paul Taylor
7. Alessia Ruggieri
8. Ioannis Smyrnias
9. Nicolas Locker

Reviewed by Review Commons

Katanin, kinesin-13, and ataxin-2 inhibit premature interaction between maternal and paternal genomes in C. elegans zygotes

1. Elizabeth A Beath
2. Cynthia Bailey
3. Meghana Mahantesh Magadam
4. Shuyan Qiu
5. Karen L McNally
6. Francis J McNally

• Curated by eLife

  eLife assessment

  This is a valuable paper that identifies a potential challenge for embryos during fertilization: holding sperm contents in the fertilized embryos away from the oocyte meiotic spindle so that they don't get ejected into the polar body during meiotic chromosome segregation. The authors identify proteins involved in cytoplasmic streaming and maintaining the grouping of paternal organelles as being critical for this process. There remain minor weaknesses in the data presented but the paper provides solid evidence for the majority of its claims, and while the findings may pertain to a narrow audience the tools used and basic characterization shown will likely be relied upon by many in the community and therefore is of high value.

Reviewed by eLife

Mapping the human hematopoietic stem and progenitor cell hierarchy through integrated single-cell proteomics and transcriptomics

1. Benjamin Furtwängler
2. Nil Üresin
3. Sabrina Richter
4. Mikkel Bruhn Schuster
5. Despoina Barmpouri
6. Henrietta Holze
7. Anne Wenzel
8. Kirsten Grønbæk
9. Kim Theilgaard-Mönch
10. Fabian J. Theis
11. Erwin M. Schoof
12. Bo T Porse

Reviewed by Arcadia Science