Cell Biology

The evolutionary origins and ancestral features of septins

1. Samed Delic
2. Brent Shuman
3. Shoken Lee
4. Shirin Bahmanyar
5. Michelle Momany
6. Masayuki Onishi

Reviewed by Arcadia Science

An evolutionary cell biology perspective into the diverging mechanisms of clathrin-mediated endocytosis in dikarya fungi

1. Andrea Picco
2. Christopher P. Toret
3. Anne-Sophie Rivier-Cordey
4. Marko Kaksonen

Reviewed by Arcadia Science

Human cytomegalovirus deploys molecular mimicry to recruit VPS4A to sites of virus assembly

1. Benjamin G. Butt
2. Daniela Fischer
3. Alison R. Rep
4. Martin Schauflinger
5. Clarissa Read
6. Thomas Böck
7. Manuel Hirner
8. Frederik Wienen
9. Stephen C. Graham
10. Jens von Einem

Reviewed by Review Commons

Distinct transcriptomic profile of satellite cells contributes to preservation of neuromuscular junctions in extraocular muscles of ALS mice

1. Ang Li
2. Jianxun Yi
3. Xuejun Li
4. Li Dong
5. Lyle W Ostrow
6. Jianjie Ma
7. Jingsong Zhou

• Curated by eLife

  eLife assessment

  The manuscript by Jingsong Zhou and colleagues uncovers why the extraocular muscles (EOMs) are preserved while other muscles undergo degenerative changes in amyotrophic lateral sclerosis (ALS). In this work, the authors have used a mouse model of familial ALS that carries a G93A mutation in the Sod1 gene to demonstrate that NaBu treatment partially restores the integrity of NMJ in the limb and diaphragm muscles of G93A mice. The findings of the study offer important information that EOMs are spared in ALS because they produce protective factors for the NMJ and, more specifically, factors secreted by EOM-derived satellite cells. While most of the experimental approaches are convincing, the use of sodium butyrate (NaBu) in this study needs further investigation, as NaBu might have a variety of biological effects. Overall, this work may help develop future therapeutic interventions for patients with ALS.

Reviewed by eLife

Structural basis of respiratory complexes adaptation to cold temperatures

1. Young-Cheul Shin
2. Pedro Latorre-Muro
3. Amina Djurabekova
4. Oleksii Zdorevskyi
5. Christopher F. Bennett
6. Nils Burger
7. Kangkang Song
8. Chen Xu
9. Vivek Sharma
10. Maofu Liao
11. Pere Puigserver

Reviewed by preLights

Nitrogen availability and TOR signalling are important for preventing catastrophic mitosis in fission yeast

1. Viacheslav Zemlianski
2. Anna Marešová
3. Jarmila Princová
4. Roman Holič
5. Robert Häsler
6. Manuel José Ramos del Río
7. Laurane Lhoste
8. Maryia Zarechyntsava
9. Martin Převorovský

Reviewed by Review Commons

Neuropilin-1 controls vascular permeability through juxtacrine regulation of endothelial adherens junctions

1. Sagnik Pal
2. Yangyang Su
3. Emmanuel Nwadozi
4. Lena Claesson-Welsh
5. Mark Richards

• Curated by eLife

  eLife assessment

  This study is focused on the question of how Nrp1 contributes to the regulation of vascular permeability and whether or why there are differences between different vascular beds. The scientific concept of this paper suggests a possible role of Nrp1 on perivascular cells as a participant in the regulation of vascular permeability. This concept is interesting and potentially useful. However, the methodology and quantitative analysis are currently inadequate to fully support the claims.

Reviewed by eLife

Senescence of endplate osteoclasts induces sensory innervation and spinal pain

1. Dayu Pan
2. Kheiria Gamal Benkato
3. Xuequan Han
4. Jinjian Zheng
5. Vijay Kumar
6. Mei Wan
7. Junying Zheng
8. Xu Cao

• Curated by eLife

  eLife assessment

  This fundamental study advances our understanding of the role of senescent osteoclasts (SnOCs) in the pathogenesis of spine instability. The authors provide compelling evidence for the SnOCs to induce sensory nerve innervation. Accordingly, reduction of SnOCs by the senolytic drug Navitoclax markedly reduces spinal pain sensitivity. This work will be of broad interest to regenerative biologists working on spinal pain.

Reviewed by eLife

Syntaxin 17 recruitment to mature autophagosomes is temporally regulated by PI4P accumulation

1. Saori Shinoda
2. Yuji Sakai
3. Takahide Matsui
4. Masaaki Uematsu
5. Ikuko Koyama-Honda
6. Jun-ichi Sakamaki
7. Hayashi Yamamoto
8. Noboru Mizushima

• Curated by eLife

  eLife assessment

  This paper addresses a fundamental issue in the field of autophagy: how is a protein responsible for autophagosome-lysosome fusion recruited to mature autophagosomes but not immature ones? The work succeeds in its ambition to provide a new conceptual advance. The evidence supporting the conclusions is convincing, with fluorescence microscopy, biochemical assays, and molecular dynamics simulations. This work will be of broad interest to cell biologists and biochemists studying autophagy, and also those focusing on lipid/membrane biology.

Reviewed by eLife

Retinal metabolism displays evidence for uncoupling of glycolysis and oxidative phosphorylation via Cori-, Cahill-, and mini-Krebs-cycle

1. Yiyi Chen
2. Laimdota Zizmare
3. Victor Calbiague
4. Lan Wang
5. Shirley Yu
6. Fritz W Herberg
7. Oliver Schmachtenberg
8. Francois Paquet-Durand
9. Christoph Trautwein

• Curated by eLife

  eLife assessment

  Chen and colleagues utilize an in situ explant model of the neural retina and retinal pigment epithelium (RPE), along with small molecule inhibition of key metabolic enzymes and targeted metabolomic analysis, to decipher key differences in metabolic pathways used by rods, cones, Muller glia, and the RPE. They conclude that rods are heavily reliant on oxidative metabolism, cones are heavily reliant on glycolysis, and multiple mechanisms exist to decouple glycolysis from oxidative metabolism in the retina. This study provides valuable metabolomic data and insights into the metabolic flexibility of different retinal cells. However, current evidence is still incomplete as several of the conclusions from the paper stand in contradiction to other published findings and the authors naturally suggests experiments that will be needed in the future to validate the hypothesized pathways and refute existing published data. Such future validation includes animal models with tissue specific knockout of the key enzymes probed in the study; inhibiting the targets of this study with more than 1 small molecule that is structurally different, and at different doses and timings; using retinal explants from matured animals; performing labeled metabolite tracing experiments; and direct assessment of mitochondrial function (via OCR) under various manipulations.

Reviewed by eLife