Cell Biology

DIO-SPOTlight Transgenic Mouse to Functionally Monitor Protein Synthesis Regulated by the Integrated Stress Response

1. Matthew L Oliver
2. Zachary F Caffall
3. Callie B Eatman
4. Timothy D Faw
5. Nicole Calakos

• Curated by eLife

  eLife Assessment

  In this manuscript, the authors describe the creation of a transgenic mouse expressing a reporter for Integrated Stress Response (ISR) activation in a CRE-dependent manner. Reliable tools for detecting ISR activation in situ are lacking, so this manuscript describes a potentially valuable tool that builds on and overcomes some of the limitations of a similar viral vector described by the authors in a previous publication. Solid evidence suggests that distinct populations of cells (ChAT) in the nervous system are marked by some level of ISR activation, and that the mouse could be most helpful as a screen for cell types in which the ISR is particularly active, although it would be difficult to draw conclusions from the reporter alone. Additional validations of the reporter activity in situ will further strengthen the manuscript.

Reviewed by eLife

Small molecule modulator of neuronal lysosome positioning and function resolves Alzheimer’s Disease-linked pathologies in cultured human neurons

1. Amanda M. Snead
2. Sruchi Patel
3. Mia Krout
4. Ryan S. Hippman
5. Gabrielle Smith
6. Diya Dileep
7. Nitya Chagoor
8. Rachel Shi
9. Ricardo Linares
10. Andrew Dobria
11. Stephanie M. Cologna
12. Camerron Crowder
13. Leslie N. Aldrich
14. Swetha Gowrishankar

Reviewed by PREreview

TET2-mediated epigenetic modification promotes stress senescence of pancreatic β cells in type 2 diabetes mellitus

1. Weijuan Cai
2. Qingqing Song
3. Xiaoqing Mo
4. Huaqian Li
5. Yuling Song
6. Liang Yin

• Curated by eLife

  eLife Assessment

  This is a useful study that seeks to address the role of the TET family of DNA demethylation enzymes in pancreatic beta cell senescence in the context of type 2 diabetes (T2DM). Although the concepts are novel and of interest, the study presents incomplete evidence to fully support its main conclusions.

Reviewed by eLife

Loss of ErbB3 redirects Integrin β1 from early endosomal recycling to secretion in extracellular vesicles

1. Dorival Mendes Rodrigues-Junior
2. Ana Rosa Sáez-Ibáñez
3. Takeshi Terabayashi
4. Nina Daubel
5. Taija Mäkinen
6. Olof Idevall-Hagren
7. Aristidis Moustakas
8. Ingvar Ferby

Reviewed by Review Commons

Single-molecule tracking reveals the dynamics of Ipl1 recruitment to the kinetochores and spindles in S. cerevisiae

1. Nitesh Kumar Podh
2. Ayan Das
3. Akriti Kumari
4. Kirti Garg
5. Rashmi Yadav
6. Kirti Kashyap
7. Sahil Islam
8. Anupam Gupta
9. Gunjan Mehta

Reviewed by Review Commons