Cell Biology

Reactive oxygen species suppress phagocyte surveillance by oxidizing cytoskeletal regulators

1. Iuliia Ferling
2. Steffen Pfalzgraf
3. Lea Moutounet
4. Lanhui Qiu
5. Yumeng Li
6. Yuhuan Zhou
7. Sergio Grinstein
8. Spencer A Freeman

• Curated by eLife

  eLife assessment

  In this study, Ferling and colleagues provide convincing evidence demonstrating that myeloid cells exert distinct, cargo-dependent responses during and after phagocytosis. These important findings establish previously unrecognized insights into the function(s) of myeloid cells in immunosurveillance and are thus likely to be broadly impactful across the spectrum of biomedical disciplines including immunology and cell biology. Notwithstanding these clear strengths of the article, some minor issues were noted pertinent to the relative opaqueness of the mechanisms underpinning context-specific RhoA activation.

Reviewed by eLife

Multi-tissue proteomics identifies a link between satellite DNA organization and heritable transposon repression in Drosophila

1. Ankita Chavan
2. Lena Skrutl
3. Federico Uliana
4. Melanie Pfister
5. Franziska Brändle
6. Laszlo Tirian
7. Delora Baptista
8. Dominik Handler
9. David Burke
10. Anna Sintsova
11. Pedro Beltrao
12. Julius Brennecke
13. Madhav Jagannathan

Reviewed by Review Commons

APEX-based proximity labeling in Plasmodium identifies a membrane protein with dual functions during mosquito infection

1. Jessica Kehrer
2. Dominik Ricken
3. Leanne Strauss
4. Emma Pietsch
5. Julia M. Heinze
6. Friedrich Frischknecht

Reviewed by Review Commons

Oversized cells activate global proteasome-mediated protein degradation to maintain cell size homeostasis

1. Shixuan Liu
2. Ceryl Tan
3. Chloe Melo-Gavin
4. Miriam B Ginzberg
5. Ron Blutrich
6. Nish Patel
7. Michael Rape
8. Kevin G Mark
9. Ran Kafri

• Curated by eLife

  Evaluation Summary:

  Kafri and colleagues assess the contribution of protein degradation to the cell size-dependent accumulation of total protein. As cells get too big, the efficiency of cell growth decreases, which the authors propose is due to increased protein degradation in larger cells. This is an interesting and novel mechanism, and its discovery is potentially useful for future research on understanding and controlling cell growth, though the data could be further strengthened and clarified to support the conclusions.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

The role of ER exit sites in maintaining P-body organization and transmitting ER stress response during Drosophila melanogaster oogenesis

1. Samantha N. Milano
2. Livia V. Bayer
3. Julie J. Ko
4. Caroline E. Casella
5. Diana P. Bratu

Reviewed by preLights

Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization

1. Martina Jabloñski
2. Guillermina M Luque
3. Matias Gomez Elias
4. Claudia Sanchez Cardenas
5. Xinran Xu
6. Jose L de La Vega Beltran
7. Gabriel Corkidi
8. Alejandro Linares
9. Victor Abonza
10. Aquetzalli Arenas-Hernandez
11. María DP Ramos-Godinez
12. Alejandro López-Saavedra
13. Dario Krapf
14. Diego Krapf
15. Alberto Darszon
16. Adán Guerrero
17. Mariano G Buffone

• Curated by eLife

  eLife assessment

  This important work substantially advances our understanding of sperm motility regulation during fertilization process by uncovering the midpiece/mitochondria contraction associated with motility cessation and structural changes in the midpiece actin network as its mode of action involved. The evidence supporting the conclusion is solid, with rigorous live cell imaging using state-of-art microscopy, although more functional analysis of the midpiece/mitochondria contraction would have further strengthened the study. The work will be of broad interest to cell biologists working on the cytoskeleton, mitochondria, cell fusion, and fertilization.

Reviewed by eLife

A Ctnnb1 enhancer transcriptionally regulates Wnt signaling dosage to balance homeostasis and tumorigenesis of intestinal epithelia

1. Xiaojiao Hua
2. Chen Zhao
3. Jianbo Tian
4. Junbao Wang
5. Xiaoping Miao
6. Gen Zheng
7. Min Wu
8. Mei Ye
9. Ying Liu
10. Yan Zhou

• Curated by eLife

  eLife assessment

  Ctnnb1 encodes β-catenin, an essential component of the canonical Wnt signaling pathway. In this important study, the authors identify an upstream enhancer of Ctnnb1 responsible for the specific expression level of β-catenin in the gastrointestinal track. Deletion of this enhancer in mice and analyses of its association with human colorectal tumors provide compelling support that it controls the dosage of Wnt signaling critical to the homeostasis in intestinal epithelia and colorectal cancers.

Reviewed by eLife

Significantly reduced, but balanced, rates of mitochondrial fission and fusion are sufficient to maintain the integrity of yeast mitochondrial DNA

1. Brett T. Wisniewski
2. Jason C. Casler
3. Laura L. Lackner

Reviewed by preLights

STAG3 promotes exit from pluripotency through post-transcriptional mRNA regulation in the cytoplasm

1. Sam Weeks
2. Dubravka Pezic
3. Martin Dodel
4. Kunal Shah
5. Amandeep Bhamra
6. Stephen Henderson
7. Silvia Surinova
8. Tyson Sharp
9. Faraz Mardakheh
10. Suzana Hadjur

• Curated by eLife

  eLife assessment

  This potentially valuable study reports new and unexpected roles of STAG3 in regulating exit from pluripotency in mouse embryonic stem cells (mESCs). However, the evidence for the proposed role of STAG3 in the post-transcriptional regulation of gene expression is viewed as yet incomplete. The work will be of interest to colleagues studying stem cells, early steps in differentiation, and gene regulation.

Reviewed by eLife

PP2A-B56 regulates Mid1 protein levels for proper cytokinesis in fission yeast

1. Madeline L. Chrupcala
2. James B. Moseley

Reviewed by Review Commons