Cell Biology

β-Arrestin-dependent and -independent endosomal G protein activation by the vasopressin type 2 receptor

1. Carole Daly
2. Akim Abdul Guseinov
3. Hyunggu Hahn
4. Adam Wright
5. Irina G Tikhonova
6. Alex Rojas Bie Thomsen
7. Bianca Plouffe

• Curated by eLife

  eLife assessment

  This is an important study that contributes to our understanding of the role of beta-arrestins in endosomal activation of the vasopressin type 2 receptors. While the use of a minigene as a tool is a weakness, the evidence is overall convincing and makes for significant findings whose theoretical and practical implications extend to other GPCRs.

Reviewed by eLife

Oral supplementation of gut microbial metabolite indole-3-acetate alleviates diet-induced steatosis and inflammation in mice

1. Yufang Ding
2. Karin Yanagi
3. Fang Yang
4. Evelyn Callaway
5. Clint Cheng
6. Martha E Hensel
7. Rani Menon
8. Robert C Alaniz
9. Kyongbum Lee
10. Arul Jayaraman

• Curated by eLife

  eLife assessment

  The studies are important to the field of hepatic steatosis and inflammation. The data provided are convincing that treatment with I3A mitigated Western diet (WD)-induced hepatic steatosis, inflammation and reversed WD-induced alterations in liver bile acids and free fatty acids in mice.

Reviewed by eLife

Development and validation of a high throughput screening platform to enable target identification in skeletal muscle cells from Duchenne Muscular Dystrophy (DMD) patients

1. Santosh Hariharan
2. Oana Lorintiu
3. Chia-Chin Lee
4. Eve Duchemin-Pelletier
5. Xianfeng Li
6. Aileen Healy
7. Regis Doyonnas
8. Luc Selig
9. Pauline Poydenot
10. Erwann Ventre
11. Andrea Weston
12. Jane Owens
13. Nicolas Christoforou

• Curated by eLife

  eLife assessment

  This is a solid methods paper developing a machine learning based protocol differentiating normal and diseased myofibers. It emerges with and validates a potentially valuable approach to diifferentiate healthy and Duchenne muscle dystrophy myofibers.

Reviewed by eLife

Crosstalk between the plasma membrane and cell-cell adhesion maintains epithelial identity for correct polarised cell divisions

1. Manal M. Hosawi
2. Jiaoqi Cheng
3. Maria Fankhaenel
4. Marcin R. Przewloka
5. Salah Elias

Reviewed by Review Commons

Phosphorylation-dependent interactions of VAPB and ELYS contribute to the temporal progression of mitosis

1. Christina James
2. Ulrike Möller
3. Sabine König
4. Henning Urlaub
5. Ralph H. Kehlenbach

Reviewed by Review Commons

Remodeling of the secretory pathway is coordinated with de novo membrane formation in budding yeast gametogenesis

1. Yasuyuki Suda
2. Hiroyuki Tachikawa
3. Tomomi Suda
4. Kazuo Kurokawa
5. Akihiko Nakano
6. Kenji Irie

Reviewed by Review Commons

Heterogeneity of radial spokes structural components and associated enzymes in Tetrahymena cilia

1. Marta Bicka
2. Corbin Black
3. Avrin Ghanaeian
4. Ewa Joachimiak
5. Anna Osinka
6. Sumita Majhi
7. Anna Konopka
8. Ewa Bulska
9. Khanh Huy Bui
10. Dorota Wloga

• Curated by eLife

  eLife assessment

  This study presents valuable new insights from the protist Tetrahymena regarding radial spokes, conserved protein complexes that are relevant for cilia motility. The work employs interdisciplinary approaches to provide convincing support for radial spoke composition with some experiments, but there are weaker areas with partially incomplete support, such as relying on knockouts alone rather than including localization studies of tagged proteins.

Reviewed by eLife

Continuous sensing of nutrients and growth factors by the mTORC1-TFEB axis

1. Breanne Sparta
2. Nont Kosaisawe
3. Michael Pargett
4. Madhura Patankar
5. Nicholaus DeCuzzi
6. John G Albeck

• Curated by eLife

  eLife assessment

  In this study, Sparta et al., generated and employed a battery of fluorescent reporters that allowed them to perform time-resolved monitoring of mechanistic target of rapamycin complex 1 (mTORC1) responses to stimuli including glucose, amino acids, and insulin at the single cell resolution. The results of this elegant approach support a model of graded mTORC1 activation in response to the aforementioned stimuli when applied individually or in combination. This model is consistent with continuous adjustment of mTORC1 signaling to changes in cellular environment and opposed to the "on/off" model of mTORC1 function. Considering the pivotal role of mTORC1 in integrating signals such as nutrients, hormones, growth factors, oxygen, and energy status with a plethora of outputs that affect cell fate and organismal physiology, it was thought that this study will be of interests across a variety of biomedical disciplines. Overall, the elegance and robustness of the approach was highly appreciated, though the paper would be strengthened by addressing some technical issues and concerns regarding the positioning of the proposed model of mTORC1 regulation in the field.

Reviewed by eLife

Stratification of enterochromaffin cells by single-cell expression analysis

1. Yan Song
2. Linda J. Fothergill
3. Kari S. Lee
4. Brandon Y. Liu
5. Ada Koo
6. Mark Perelis
7. Shanti Diwakarla
8. Brid Callaghan
9. Jie Huang
10. Jill Wykosky
11. John B. Furness
12. Gene W. Yeo

• Curated by eLife

  eLife assessment

  This valuable study presents a solid transcriptomic analysis of enterochromaffin cells, but there are weaknesses in the functional and physiological data describing the role of enterochromaffin cell mechanosensory receptors (Piezo2 channels) in regulating colonic motility.

Reviewed by eLife

Mechano-dependent sorbitol accumulation supports biomolecular condensate

1. Stephanie Torrino
2. William M Oldham
3. Andrés R Tejedor
4. Ignacio S. Burgos
5. Nesrine Rachedi
6. Kéren Fraissard
7. Caroline Chauvet
8. Chaima Sbai
9. Brendan P. O’Hara
10. Sophie Abélanet
11. Frederic Brau
12. Stephan Clavel
13. Rosana Collepardo-Guevara
14. Jorge R. Espinosa
15. Issam Ben-Sahra
16. Thomas Bertero

Reviewed by preLights