Cell Biology

Quantitative phase imaging with temporal kinetics predicts hematopoietic stem cell diversity

1. Takao Yogo
2. Yuichiro Iwamoto
3. Hans Jiro Becker
4. Takaharu Kimura
5. Ayano Sugiyama-Finnis
6. Tomomasa Yokomizo
7. Toshio Suda
8. Sadao Ota
9. Satoshi Yamazaki

Reviewed by Arcadia Science

The exocyst complex controls multiple events in the pathway of regulated exocytosis

1. Sofía Suárez Freire
2. Sebastián Perez-Pandolfo
3. Sabrina Micaela Fresco
4. Julián Valinoti
5. Eleonora Sorianello
6. Pablo Wappner
7. Mariana Melani

• Curated by eLife

  eLife Assessment

  This study makes an important contribution by characterizing the role of the exocyst in secretory granule exocytosis in the Drosophila larval salivary gland. The results are solid and lead to the novel interpretation that the exocyst participates not only in exocytosis, but also in earlier steps of secretory granule biogenesis and maturation. However, the authors are urged to provide additional proof that the exocyst subunit knockdowns were effective and to acknowledge the possibility that inactivation of an essential exocytosis component could indirectly affect other parts of the secretory pathway.

Reviewed by eLife

Multi-color fluorescence live-cell imaging in Dictyostelium discoideum

1. Hidenori Hashimura
2. Satoshi Kuwana
3. Hibiki Nakagwa
4. Kenichi Abe
5. Tomoko Adachi
6. Toyoko Sugita
7. Shoko Fujishiro
8. Gen Honda
9. Satoshi Sawai

Reviewed by Arcadia Science

Genome concentration limits cell growth and modulates proteome composition in Escherichia coli

1. Jarno Mäkelä
2. Alexandros Papagiannakis
3. Wei-Hsiang Lin
4. Michael Charles Lanz
5. Skye Glenn
6. Matthew Swaffer
7. Georgi K Marinov
8. Jan M Skotheim
9. Christine Jacobs-Wagner

• Curated by eLife

  eLife Assessment

  This fundamental work by Mäkelä et al. presents compelling experimental evidence supported by a theoretical model that the amount of chromosomal DNA can become limiting for the total rate of mRNA transcription and consequently protein production in the model bacterium Escherichia coli. The work is based on a mutant that allows inhibition of DNA replication while following growth at the single-cell level due to cell filamentation. The work significantly advances our understanding of growth and of the central dogma, and will be of considerable interest within both systems biology and microbial physiology.

Reviewed by eLife

Single-nucleus transcriptomics reveal the differentiation trajectories of periosteal skeletal/stem progenitor cells in bone regeneration

1. Simon Perrin
2. Maria Ethel
3. Vincent Bretegnier
4. Cassandre Goachet
5. Cécile-Aurore Wotawa
6. Marine Luka
7. Fanny Coulpier
8. Cécile Masson
9. Mickael Ménager
10. Céline Colnot

• Curated by eLife

  eLife Assessment

  This fundamental study generated a single cell atlas of mouse periosteal cells under both steady-state and fracture healing conditions to address the knowledge gap regarding cellular composition of the periosteum and their responses to injury. Based on convincing transcriptome analyses and experimental validation, the authors identified the injury induced fibrogenic cell (IIFC) as a characteristic cell type appearing in the bone regeneration process and proposed that the IIFC is a progenitor undergoing osteochondrogenic differentiation. This study will provide a significant publicly accessible dataset to reexamine the expression of the reported periosteal stem and progenitor cell markers.

Reviewed by eLife

Long range mutual activation establishes Rho and Rac polarity during cell migration

1. Henry De Belly
2. Andreu Fernandez Gallen
3. Evelyn Strickland
4. Dorothy C. Estrada
5. Patrick J. Zager
6. Tamas L. Nagy
7. Janis K Burkhardt
8. Hervé Turlier
9. Orion D. Weiner

Reviewed by preLights

Intracellular diffusion in the cytoplasm increases with cell size in fission yeast

1. Catherine Tan
2. Michael C. Lanz
3. Matthew Swaffer
4. Jan Skotheim
5. Fred Chang

Reviewed by preLights

PKA regulation of neuronal function requires the dissociation of catalytic subunits from regulatory subunits

1. Weihong Xiong
2. Maozhen Qin
3. Haining Zhong

• Curated by eLife

  eLife Assessment

  This important paper demonstrates that different PKA subtypes exhibit distinct subcellular localization at rest in CA1 neurons. The authors provide compelling evidence that when all tested PKA subtypes are activated by norepinephrine, catalytic subunits translocate to dendritic spines but regulatory subunits remain unmoved. Furthermore, PKA-dependent regulation of synaptic plasticity and transmission can be supported only by wildtype, dissociable PKA, but not by inseparable PKA.

Reviewed by eLife

Spatiotemporal recruitment of the ubiquitin-specific protease USP8 directs endosome maturation

1. Yue Miao
2. Yongtao Du
3. Baolei Wang
4. Jingjing Liang
5. Yu Liang
6. Song Dang
7. Jiahao Liu
8. Dong Li
9. Kangmin He
10. Mei Ding

• Curated by eLife

  eLife Assessment

  The manuscript presents an important model for the field of endosome maturation, providing perspective on the role of the deubiquitinating enzyme UPS-50/USP8 in the process. The evidence presented in the paper is clear, incorporating well-designed experiments that suggest the dual actions of UPS-50 and USP8 in the conversion of early endosomes into late endosomes. Overall, the work is convincing and centers on an intriguing subject.

Reviewed by eLife

Non-disruptive inducible labeling of ER-membrane contact sites using the Lamin B Receptor

1. Laura Downie
2. Nuria Ferrandiz
3. Megan Jones
4. Stephen J. Royle

Reviewed by preLights