Cell Biology

Clathrin-independent endocytosis and retrograde transport in cancer cells tune immune synapse organization and CD8 T cell response

1. Shiqiang Xu
2. Alix Buridant
3. Thibault Hirsch
4. Céline Duhamel
5. Benjamin Ledoux
6. Massiullah Shafaq-Zadah
7. Estelle Dransart
8. Louise Thines
9. Ludger Johannes
10. Pierre Van Der Bruggen
11. Pierre Morsomme
12. Henri-François Renard

• Curated by eLife

  eLife Assessment

  This study now provides solid evidence for a role of EndoA3-mediated trafficking of ICAM-1 to the immune synapse with T cells. The study will be valuable to those studying cell-cell communication in the immune system, and opens additional questions regarding the mechanisms involved and how other adhesion ligands are regulated.

Reviewed by eLife

GTPase activating protein DLC1 spatio-temporally regulates Rho signaling

1. Lucien Hinderling
2. Max Heydasch
3. Giliane Rochat
4. Laurent Dubied
5. Jakobus van Unen
6. Maciej Dobrzynski
7. Olivier Pertz

• Curated by eLife

  eLife Assessment

  This study presents a valuable finding on how the GAP DLC1, a deactivator of the small GTPase RhoA, regulates RhoA activity globally as well as at Focal Adhesions. Using a new acute optogenetic system coupled to a RhoA activity biosensor, the authors present convincing evidence that DLC1 amplifies local Rho activity at Focal Adhesions. Thanks to modeling, they show that DLC1 is needed for a negative feedback loop that engage more RhoA deactivators upon RhoA activation, highlighting the complex regulation of RhoGTPases in space and time.

Reviewed by eLife

Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrow

1. Jing-Xin Feng
2. Mei-Ting Yang
3. Lili Li
4. Caiyi C Li
5. Ferenc Livák
6. Jack Chen
7. Yongmei Zhao
8. Dunrui Wang
9. Avinash Bhandoola
10. Naomi Taylor
11. Giovanna Tosato

• Curated by eLife

  eLife Assessment

  This manuscript by Feng et al. provides valuable evidence regarding the hematopoietic differentiation of bone marrow endothelial cells in the adult mouse. Overall, the authors have addressed our main concerns. Solid data now more strongly support long-term multi-lineage reconstitution of the adult hemogenic endothelial cells. However, critical data, especially regarding the endothelial cells' hematopoietic identity and functional capacity, remain insufficient, which limits the strength of the hemogenic claim, especially the assertion that these adult hemogenic ECs generate bona fide HSCs. Additional experiments would be necessary to fully rule out alternative explanations.

Reviewed by eLife

Cell size modulates ferroptosis susceptibility

1. Evgeny Zatulovskiy
2. Magdalena B. Murray
3. Shuyuan Zhang
4. Scott J. Dixon
5. Jan M. Skotheim

Reviewed by Review Commons

Epigenetics and chromatin structure regulate var2csa expression and the placental binding phenotype in Plasmodium falciparum

1. Todd Lenz
2. Madle Sirel
3. Hannes Hoppe
4. Sulman Shafeeq
5. Karine Le Roch
6. Ulf Ribacke

• Curated by eLife

  eLife Assessment

  This interesting study presents a multi-OMICs approach to unify different lines of evidence regarding the epigenetic regulation of the key virulence factor causing placental malaria during P. falciparum infection. Most results are confirmatory of previous observations; nonetheless, the claims are supported by convincing evidence. The combinatorial approach chosen here is unprecedented and therefore provides valuable new data. In addition, the comparative investigation of different DNA methylation modifications is novel and disproves a direct role in var gene regulation.

Reviewed by eLife

Fluorescence Lifetime Unmixing: A New Workflow for FLIM Live-Cell Imaging

1. Cornelia Wetzker
2. Marcelo Leomil Zoccoler
3. Svetlana Iarovenko
4. Chukwuebuka William Okafornta
5. Anja Nobst
6. Hella Hartmann
7. Thomas Müller-Reichert
8. Robert Haase
9. Gunar Fabig

Reviewed by Arcadia Science

Quantitative comparison of PI(3,5)P ₂ biosensors reveals SnxA is the most sensitive and unbiased

1. Tiernan Swayhoover
2. Claire C. Weckerly
3. Gerald R. V. Hammond

Reviewed by Review Commons

Cell loss disrupts mechanical homeostasis to drive retinal pigment epithelium ageing-like phenotype in vitro

1. Teodora Piskova
2. Aleksandra N. Kozyrina
3. Giedrė Astrauskaitė
4. Mohamed Elsafi Mabrouk
5. Sebastian Schepl
6. Stacy Lok Sze Yam
7. Ragul Ravithas
8. Wolfgang Wagner
9. Massimo Vassalli
10. Jacopo Di Russo

Reviewed by preLights

CD8 T lymphocytes redeploy embryonic cell cycle control mechanisms to facilitate rapid cell proliferation

1. D. A. Lewis
2. A. Kar
3. A. Savage
4. V. Kelly
5. D. Wright
6. R. Zamoyska
7. T. Ly

Reviewed by Review Commons

Contractile peri-nuclear actomyosin network repositions peripheral and polar chromosomes to promote early kinetochore–microtubule interactions

1. Nooshin Sheidaei
2. John K Eykelenboom
3. Zuojun Yue
4. Graeme Ball
5. Alexander JR Booth
6. Tomoyuki U Tanaka

• Curated by eLife

  eLife Assessment

  This important study demonstrates that a peri-nuclear actomyosin network, present in some types of human cells, facilitates kinetochore-spindle attachment of chromosomes in unfavorable locations - thereby suppressing their missegregation rate. This actomyosin network and its general role have been studied previously, but this study convincingly clarifies the underlying mechanism using a light-controlled perturbation and detailed tracking of kinetochore movement. The generality of the mechanism could be further supported by confirming the findings in non-synchronized cells and additional cell lines. The results may have implications for understanding chromosome missegregation in cancer cells.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons