Cell Biology

Centrosome Migration and Apical Membrane Formation in Polarized Epithelial Cells: Insights from the MDCK Cyst Model

1. Po-Kai Wang
2. Keng-Hui Lin
3. Tang K Tang

• Curated by eLife

  eLife Assessment

  This important study contributes to our understanding of how epithelial cells establish polarity by identifying a hierarchy in which Par3 acts upstream of centrosome positioning and apical membrane initiation. The evidence supporting the main conclusions is convincing, although several aspects of the model remain only partially supported due to unresolved questions about microtubule organization and the need for clearer integration of quantitative and conceptual points raised in review. The work will be of interest to cell and developmental biologists, but the conclusions would be strengthened by greater precision in methodology, terminology, and interpretation.

Reviewed by eLife

A conserved Hsp70 phosphorylation regulates cell cycle progression after DNA damage

1. Thomas Moss
2. Alexandra Wooldredge
3. Koustav Bhakta
4. Matthew Cronin
5. Jason E Gestwicki
6. Shaeri Mukherjee

• Curated by eLife

  eLife Assessment

  This potentially valuable manuscript focuses on the phosphorylation of residue T495 as a mechanism to inactivate HSP70 and disrupt cell cycle progression in response to DNA damage. The evidence supporting this model is incomplete and would be strengthened by additional studies defining the extent of T495 phosphorylation induced by DNA damage, identifying the kinase responsible for phosphorylating T495 of HSP70, and further elucidation of the functional implications of T495 phosphorylation in human cells. This work will be of interest to scientists focused on topics including chaperone biology, proteostasis, cell cycle progression, and DNA damage.

Reviewed by eLife, preLights

MIRO1 controls energy production and proliferation of vascular smooth muscle cells

1. Lan Qian
2. Olha M Koval
3. Benney T Endoni
4. Denise Juhr
5. Colleen S Stein
6. Chantal Allamargot
7. Li-Hsien Lin
8. Deng-Fu Guo
9. Kamal Rahmouni
10. Antentor O Hinton
11. E Dale Abel
12. Ryan L Boudreau
13. Jennifer Streeter
14. William H Thiel
15. Isabella M Grumbach

• Curated by eLife

  eLife Assessment

  The findings are important, as they identify MIRO1 as a central regulator linking mitochondrial positioning and respiratory chain function to VSMC proliferation, neointima formation, and human vasoproliferative disease. Overall, the strength of evidence is convincing, with comprehensive in vivo and in vitro data, including human cells and added bioenergetic analyses, that broadly support the main claims despite some remaining limitations in mechanistic and mitochondrial assays.

Reviewed by eLife

PIP5K-Ras bistability triggers plasma membrane symmetry breaking to define cellular polarity and regulate migration

1. Yu Deng
2. Tatsat Banerjee
3. Satomi Matsuoka
4. Debojyoti Biswas
5. Liz A. Kurtz
6. Jane Borleis
7. Yu Long
8. Parijat Banerjee
9. Huiwang Zhan
10. Dhiman Sankar Pal
11. Nathan H. Roy
12. Masahiro Ueda
13. Pablo A. Iglesias
14. Peter N. Devreotes

Reviewed by preLights

VAMP8 function reveals tight linkage between endocytic recycling and endocytosis

1. Ailing Liu
2. Yueping Li
3. Zheng Huang
4. Wen Chen
5. Peiliu Xu
6. Xiangying Wei
7. Guosheng Hu
8. Shuangquan Liu
9. Xiaoxia Liu
10. Yaohui He
11. Danling Wang
12. Sandra L Schmid
13. Zhiming Chen

Reviewed by Review Commons

Ubiquitination regulates granulostasis and DRiP accumulation in SGs under heat stress via the E3 ligase MKRN2

1. Emmanuel Amzallag
2. Yehuda M. Danino
3. Valentina Secco
4. Serena Carra
5. Eran Hornstein

Reviewed by Review Commons

Enteropathogenic E. coli-mediated Fast and Coordinated Ca²⁺ responses regulate NF-κB activation

1. Fangrui Guo
2. Roberto Ornelas Guevara
3. Linda Oussaedine
4. Geneviève Dupont
5. Laurent Combettes
6. Guy Tran Van Nhieu

• Curated by eLife

  eLife Assessment

  This study reports important advances in our understanding of how enteropathogenic E. coli (EPEC) interacts at the intestinal interface. Solid data describe a novel model of spatially coordinated calcium signaling to modulate NF-kB activation; additional data and clarification of methods would improve the strength of these conclusions. These findings, which integrate imaging, genetics, and computational modeling, provide a new way to consider host-pathogen interactions in EPEC infections that may lead to improved therapies.

Reviewed by eLife

A retrograde transit filter mediated by optineurin controls mitostasis in distal axons

1. Natalia A. Marahori
2. Barbara Gailer
3. Martina Schifferer
4. Tatjana Kleele
5. Anna Iatroudi
6. Shabab B. Hannan
7. Petros Avramopoulos
8. Stefan Engelhardt
9. Melike Lakadamyali
10. Monika S. Brill
11. Thomas Misgeld

Reviewed by PREreview

mTORC1 signaling modulate microtubule tyrosination/detyrosination status to regulate lysosome dynamics

1. Jeganath Ammavasai
2. Nitin Mohan

Reviewed by PREreview

Identification of a sub-population of synovial mesenchymal stem cells with enhanced treatment efficacy in a rat model of Osteoarthritis

1. Nedaa Al-Jezani
2. Asmaa Affan
3. Catherine Leonard
4. Nabangshu Das
5. Luiz Gustavo Almeida
6. Daniel Young
7. Anand O Masson
8. Antoine Dufour
9. Paul Salo
10. Pam Railton
11. James N Powell
12. Roman J Krawetz

• Curated by eLife

  eLife Assessment

  This important study characterized and identified clonal MSC populations from human synovium. The authors provide convincing evidence that clonal MSC populations can be isolated and expanded from both normal and osteoarthritic synovium and that CD47 represents a potential marker for improved chondrogenic potential of MSC sub-populations. These findings could provide new avenues for osteoarthritis treatment in the future and deeper mechanistic understanding of the factors involved in the repair.

Reviewed by eLife