Cell Biology

Human formin FHOD3-mediated actin elongation is required for sarcomere integrity in cardiomyocytes

1. Dylan A Valencia
2. Angela N Koeberlein
3. Haruko Nakano
4. Akos Rudas
5. Aanand A Patel
6. Airi Harui
7. Cassandra Spencer
8. Atsushi Nakano
9. Margot E Quinlan

• Curated by eLife

  eLife Assessment

  Valencia et al. combine elegant in vitro biochemical experiments with functional assays in cardiomyocytes to determine which properties of the FHOD3 formin are essential for sarcomere assembly. Using separation-of-function mutants, they show that FHOD3's elongation activity, rather than its nucleation, capping, or bundling activities, is key to its sarcomeric function. This is an important finding and the data presented in the manuscript are convincing; however, the presence of FHOD3 at filament barbed ends in the TIRF elongation assays should probably be verified directly in a future study.

Reviewed by eLife

Mitochondrial fission during mitophagy requires both inner and outer mitofissins

1. Kentaro Furukawa
2. Tatsuro Maruyama
3. Shun-ichi Yamashita
4. Keiichi Inoue
5. Tomoyuki Fukuda
6. Nobuo N. Noda
7. Tomotake Kanki

Reviewed by Review Commons

Cardiac neurons expressing a glucagon-like receptor mediate cardiac arrhythmia induced by high-fat diet in Drosophila

1. Yunpo Zhao
2. Jianli Duan
3. Joyce van de Leemput
4. Zhe Han

• Curated by eLife

  eLife Assessment

  This study reports useful information on the mechanisms by which a high-fat diet induces arrhythmias in the model organism Drosophila. Specifically, the authors propose that adipokinetic hormone (Akh) secretion is increased with this diet, and through binding of Akh to its receptor on cardiac neurons, arrhythmia is induced. The authors have revised their manuscript but the evidence remains incomplete. Nonetheless, the data presented will be helpful to those who wish to extend the research to a more complex model system, such as the mouse.

Reviewed by eLife

Quantitative intra-Golgi transport and organization data suggest the stable compartment nature of the Golgi

1. Hieng Chiong Tie
2. Haiyun Wang
3. Divyanshu Mahajan
4. Hilbert Yuen In Lam
5. Xiuping Sun
6. Bing Chen
7. Yuguang Mu
8. Lei Lu

• Curated by eLife

  eLife Assessment

  This important study offers convincing evidence that intra-Golgi transport slows from cis to trans and varies between cargos even within the same cisternae, supporting a more stable compartment model. Using nocodazole-induced ministacks, the authors show cargo-specific transport kinetics with distinct velocities and residence times. These findings refine the cisternal progression model and prompt further investigation into alternative mechanisms, such as rapid partitioning or rim progression. This study will be of interest to cell biologists studying membrane trafficking, Golgi organization, and protein secretion, as well as researchers investigating the mechanisms of organelle dynamics and the molecular basis of intracellular transport.

Reviewed by eLife

CCDC32 stabilizes clathrin-coated pits and drives their invagination

1. Ziyan Yang
2. Changsong Yang
3. Zheng Huang
4. Peiliu Xu
5. Yueping Li
6. Lu Han
7. Linyuan Peng
8. Xiangying Wei
9. John Pak
10. Tatyana Svitkina
11. Sandra L. Schmid
12. Zhiming Chen

• Curated by eLife

  eLife Assessment

  The manuscript presents a valuable finding that CCDC32, beyond its reported role in AP2 assembly, follows AP2 to the plasma membrane and regulates clathrin-coated pit assembly and dynamics. The authors further suggest that the alpha-helical region of CCDC32 interacts with AP2 via the alpha appendage domain to mediate this function. While live-cell and ultrastructural imaging data are solid, future biochemical studies will be needed to confirm the proposed CCDC32-AP2 interaction.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

Optogenetic actin network assembly on lipid bilayer uncovers the network density-dependent functions of actin-binding proteins

1. Kei Yamamoto
2. Makito Miyazaki

Reviewed by Arcadia Science

A Commander-independent function of COMMD3 in endosomal trafficking

1. Galen T Squiers
2. Chun Wan
3. James Gorder
4. Harrison Puscher
5. Jingshi Shen

• Curated by eLife

  eLife Assessment

  This important study explores the mechanisms underlying the maintenance of cell surface protein levels. The authors present solid evidence to support their claims, though the addition of certain validation experiments could have further strengthened the conclusions. This work will be of particular interest to cell biologists focused on membrane trafficking.

Reviewed by eLife

The triad interaction of ULK1, ATG13, and FIP200 is required for ULK complex formation and autophagy

1. Yutaro Hama
2. Yuko Fujioka
3. Hayashi Yamamoto
4. Noboru Mizushima
5. Nobuo N Noda

• Curated by eLife

  eLife Assessment

  Building on previous structural studies, this work provides valuable new insights into the architecture of the autophagy initiation complex, comprising ULK1, ATG13, and FIP200. The authors present their findings with solid supporting evidence, making this study a significant contribution to the autophagy field.

Reviewed by eLife

Reversible One-way Lipid Transfer at ER–Autophagosome Membrane Contact Sites via Bridge-like Lipid Transfer Protein Atg2

1. Li Hao
2. Tomoki Midorikawa
3. Yuta Ogasawara
4. Yutaro Hama
5. Huichao Lang
6. Nobuo N. Noda
7. Kuninori Suzuki

Reviewed by Review Commons

Secreted exosomes induce filopodia formation

1. Caitlin McAtee
2. Mikin Patel
3. Daisuke Hoshino
4. Bong Hwan Sung
5. Ariana von Lersner
6. Mingjian Shi
7. Nan Hyung Hong
8. Anna Young
9. Evan Krystofiak
10. Andries Zijlstra
11. Alissa M Weaver

• Curated by eLife

  eLife Assessment

  In this important manuscript, the authors reveal novel findings on the role of exosomes in regulating filopodia formation. Filopodia are crucial for various cellular processes, including migration, polarization, directional sensing, and the formation of neuronal synapses. The authors convincingly demonstrate that exosomes, particularly those enriched with the protein THSD7A, play a significant role in promoting filopodia formation in both cancer cells and neurons.

Reviewed by eLife