Cell Biology

Defective Neuronal Differentiation in Lowe Syndrome is Associated with Mitochondrial Dysfunction and Impaired Cilia-related Sonic Hedgehog Signaling

1. Grzegorz Walkiewicz
2. Siyu Chen
3. Chien-Hui Lo
4. Jingyu Zhao
5. Zhiquan Liu
6. Biao Wang
7. Benjamin Lawson
8. Qing Wang
9. Tia J Kowal
10. Yang Sun

• Curated by eLife

  eLife Assessment

  This study investigated mitochondrial dysfunction and the impairment of the ciliary Sonic Hedgehog signaling in Lowe syndrome (LS), a timely topic given the limited research in this area. The data obtained from patient-derived iPSC neurons and a mouse model are solid. Although the main claims of the study are only partially supported by the current evidence, it provides a useful starting point for future functional studies investigating the link between mitochondrial defects and primary cilia in neural development.

Reviewed by eLife

EPHA1 and EPHB4 tyrosine kinase receptors regulate epithelial morphogenesis

1. Noémie Lavoie
2. Anaëlle Scribe
3. François J.M. Chartier
4. Karim Ghani
5. Alexandra Jetté
6. Sara L. Banerjee
7. Manuel Caruso
8. Mélanie Laurin
9. Andrew Freywald
10. Sabine Elowe
11. Patrick Laprise
12. Nicolas Bisson

Reviewed by Review Commons

Nim1-related kinases regulate septin organization and cytokinesis by modulating Hof1 at the cell division site

1. Bindu Bhojappa
2. Anubhav Dhar
3. VT Bagyashree
4. Jayanti Kumari
5. Freya Cardozo
6. Vaseef Rizvi
7. Deepthi Guturu
8. Saravanan Palani

• Curated by eLife

  eLife Assessment

  In this valuable study, Bhojappa et al. investigate the function of four septin-associated kinases (Elm1p, Gin4p, Hsl1p and Kcc4p) in controlling septin ring stability, actomyosin ring organization and constriction, and cytokinesis in budding yeast. The evidence supporting the conclusions is convincing. The results are a meaningful addition to previously published work and provide a clearer picture of a complex mechanism involving proteins of partially redundant functions.

Reviewed by eLife

Cdk1 and PP2A constitute a molecular switch controlling orderly degradation of atypical E2Fs

1. Sapir Nachum-Raines
2. Noy Gamliel
3. Danit Wasserman
4. Nasrin Qassem
5. Inbal Sher
6. Julia Guez-Haddad
7. Michael J Emanuele
8. Jordan H Chill
9. Amit Tzur

Reviewed by Review Commons

Human mitochondrial DNA variants influence telomere length: evidence from a transmitochondrial cybrid model

1. Manon Mahieu
2. Jean-Philippe Defour
3. Barbara Mathieu
4. Elena Richiardone
5. Isaac Heremans
6. Elisa Fabiole
7. Gabriel Levy
8. Gabriel Le Berre
9. Isabelle Scheers
10. Bénédicte Brichard
11. Thierry Arnould
12. Patrick Revy
13. Guido Bommer
14. Bernard Gallez
15. Cyril Corbet
16. Anabelle Decottignies

• Curated by eLife

  eLife Assessment

  This important study addresses a timely issue at the intersection of mitochondrial and telomere biology by focusing on the relationship between naturally occurring variants in the mitochondrial genome and telomere length. This work thereby provides a conceptual and experimental framework for investigating communication between mitochondria and telomeres. Using an innovative transmitochondrial cybrid approach, the authors provide evidence that mitochondrial DNA variants influence telomere maintenance through effects on mitochondrial function, reactive oxygen species, and NAD⁺-dependent repair processes. The evidence supporting the central conclusion that mitochondrial genotype influences telomere-associated phenotypes is convincing and is strengthened by the use of complementary functional and rescue experiments. However, some of the mechanistic interpretations and broader conclusions regarding telomere length inheritance in humans would benefit from additional donors and longitudinal analyses following cybrid generation, or more cautious framing.

Reviewed by eLife

PXGS: a Poly-Transgene Expression System based on Mutually Exclusive Splicing of Dscam

1. Renee Yin Yu
2. Alyeri Bucio-Méndez
3. Brian E Chen

• Curated by eLife

  eLife Assessment

  This valuable study describes PXGS, a poly-transgene expression system that exploits the mutually exclusive splicing of Dscam variable exon 4 to enable conditional, simultaneous expression of up to 12 transgenes in Drosophila, addressing a longstanding limitation in which conditional co-expression has been restricted to a handful of genes. The approach is conceptually elegant and technically accessible, with potential applications spanning neuroscience, synthetic biology, and biomanufacturing across arthropod species. The evidence that Dscam exon 4 splicing is preserved in a UAS vector and that individual alternates can be replaced with functional transgenes is solid, and the in vivo axonal re-wiring application provides a convincing proof of principle. Quantitative characterization of expression levels, a direct demonstration of expression across all twelve positions, and additional imaging controls would further substantiate the system's utility and scope.

Reviewed by eLife

MR1 dependent MAIT cell activation is regulated by autophagy associated proteins

1. Prabhjeet Phalora
2. James Ussher
3. Svenja Hester
4. Carl-Philipp Hackstein
5. Emanuele Marchi
6. Jeffrey Y. W. Mak
7. David P. Fairlie
8. Paul Klenerman

Reviewed by Review Commons

The cell cycle variant in multiciliated cells incorporates 2 centriole biogenesis cycles

1. Amélie-Rose Boudjema
2. Rémi Balagué
3. Cayla E Jewett
4. Jacques Serizay
5. Gina M LoMastro
6. Olivier Mercey
7. Adel Al Jord
8. Adrien Candat
9. Marion Faucourt
10. Alexandre Schaeffer
11. Camille Noûs
12. Nathalie Delgehyr
13. Andrew J Holland
14. Nathalie Spassky
15. Alice Meunier

• Curated by eLife

  eLife Assessment

  In this important study, Boudjema et al. use cell culture models and high quality advanced microscopic imaging to provide detailed analyses of the cellular processes underlying centriole amplification, apical migration, and assembly of hundreds of motile cilia in multi-ciliated cells. The authors present convincing evidence showing that in these cells all the molecular and cellular steps controlling centriole biogenesis that in cycling cells extend over almost two cell cycles, occur within a single cell cycle variant. This work provides a better understanding of the regulation and order of these processes and is of interest to all cell biologists and in particular researchers studying centrioles and cilia.

Reviewed by eLife

Cell cycle dependent variation in endocytosis drives phenotypic diversity in M. tuberculosis

1. Neeraja Subhash
2. Sandhya Krishnan Radhakrishnan
3. Hitakshi Vijay
4. Neilay Bhalerao
5. Sahanawaz Molla
6. Anton Iyer
7. Shaon Chakrabarti
8. Varadharajan Sundaramurthy

• Curated by eLife

  eLife Assessment

  This useful study explores how macrophage cell-cycle state may influence endocytosis, Mycobacterium tuberculosis uptake, and the intracellular stress experienced by bacteria. While the question is interesting and the experimental approach has promise, the evidence for the central claim that endocytic capacity is specifically regulated by cell-cycle stage is incomplete. The main concern is that fluorescence-based sorting and total-fluorescence measurements likely covary with cell size, so the reported phenotypes could reflect biomass accumulation or other cell-cycle-associated changes rather than endocytic capacity as the causal determinant. As a result, whilst the study raises a hypothesis that is of importance, additional controls are required before the proposed mechanism can be considered well supported.

Reviewed by eLife