Cell Biology

HAK-actin, U-ExM-compatible probe to image the actin cytoskeleton

1. Olivier Mercey
2. Luc Reymond
3. Florent Lemaître
4. Isabelle Mean
5. Marine H. Laporte
6. Marine Olivetta
7. Karin Sadoul
8. Omaya Dudin
9. Virginie Hamel
10. Paul Guichard

Reviewed by preLights

Condensin II collaborates with cohesin to establish and maintain interphase chromosome territories

1. Takao Ono
2. Masatoshi Takagi
3. Hideyuki Tanabe
4. Tomoko Fujita
5. Noriko Saitoh
6. Akatsuki Kimura
7. Tatsuya Hirano

Reviewed by Review Commons

The dynamics of ciliogenesis in prepubertal mouse meiosis reveal new clues about testicular maturation during puberty

1. I Pérez-Moreno
2. P López-Jiménez
3. H Zapata
4. S Pérez-Martín
5. M López-Panadés
6. B Barbeito
7. J Urtasun-Elizari
8. I Roig
9. FR Garcia-Gonzalo
10. J Page
11. R Gómez

Reviewed by Review Commons

The Anti-Inflammatory Role of GPNMB in Post-Traumatic Osteoarthritis

1. Asaad A Al-Adlaan
2. Bryson Cook
3. Nazar J Hussein
4. Fatima A Jaber
5. Trinity Kronk
6. Ernesto Solorzano Z
7. Salvatore Frangiamore
8. Hope C Ball
9. Fayez F Safadi

• Curated by eLife

  eLife Assessment

  This study demonstrates the cartilage-protective effects of osteoactivin in inflammatory experimental models. The work offers valuable insights advancing current knowledge regarding regulation of joint inflammation and tissue degeneration. The evidence provided is compelling and suggests that osteoactivin may serve as a promising therapeutic target for inflammatory joint diseases.

Reviewed by eLife

Defining the chromatin-associated protein landscapes on Trypanosoma brucei repetitive elements using synthetic TALE proteins

1. Roberta Carloni
2. Tadhg Devlin
3. Pin Tong
4. Christos Spanos
5. Tanya Auchynnikava
6. Juri Rappsilber
7. Keith R. Matthews
8. Robin C. Allshire

Reviewed by Review Commons

Acidocalcisome-like vacuoles constitute a feedback-controlled phosphate buffering system for the cytosol

1. Samuel Bru
2. Lydie Michaillat Mayer
3. Geun-Don Kim
4. Danye Qiu
5. Henning Jacob Jessen
6. Andreas Mayer

• Curated by eLife

  eLife Assessment

  This study provides fundamental insights into eukaryotic phosphate homeostasis by demonstrating how yeast vacuoles dynamically regulate cytosolic phosphate levels. The conclusions are convincing, supported by an elegant combination of in vitro assays and in vivo measurements. This study will be of interest to cell biologists, particularly for those who are working in the field of phosphate metabolism.

Reviewed by eLife

Labeling of Nascent RNA in C. elegans Intestine

1. Omid Gholamalamdari
2. Stephanie C. Weber

Reviewed by Arcadia Science

SMC5/6-Mediated Plasmid Silencing is Directed by SIMC1-SLF2 and Antagonized by the SV40 Large T Antigen

1. Martina Oravcová
2. Minghua Nie
3. Takanori Otomo
4. Michael N Boddy

• Curated by eLife

  eLife Assessment

  This Research Advance manuscript further elucidates the roles of SMC5/6 loader proteins and associated factors in the silencing of extrachromosomal circular DNA by the SMC5/6 complex. While the findings are largely in line with expectations, they are valuable, representing a meaningful advance beyond the recent study from the same laboratories (PMC9708086), validating the previous model that distinct SMC5/6 subcomplexes, SIMC1-SLF2 and SLF1/2, separately control its transcriptional repression and DNA repair activities on extrachromosomal DNA. Solid evidence is presented for a role for SIMC1/SLF2 in localization of the SMC5/6 complex to plasmid DNA, and the distinct requirements as compared to recruitment of SMC5/6 to chromosomal DNA lesions.

Reviewed by eLife

TRIM32 controls timely cell cycle exit in muscular differentiation through c-Myc down-regulation

1. Lu Xiong
2. Elisa Lazzari
3. Sabrina Pacor
4. Simeone Dal Monego
5. Erica Piovesan
6. Danilo Licastro
7. Germana Meroni

Reviewed by Review Commons

Vesiculation pathways in clathrin-mediated endocytosis

1. Xinran Wang
2. Julien Berro
3. Rui Ma

• Curated by eLife

  eLife Assessment

  This valuable study proposes a theoretical model of clathrin coat formation based on membrane elasticity that seeks to determine whether this process occurs by increasing the area of a protein-coated patch with constant curvature, or by increasing the curvature of a protein-coated patch that forms in an initially flat conformation (so called constant curvature or constant area models). Identifying energetically favorable pathways and comparing the obtained shapes with experiments provides solid support to the constant-area pathway. This work will be of interest for biologists and biophysicists interested in membrane remodelling and endocytosis. It provides an innovative approach to tackle the question of constant curvature vs. constant area coat protein formation, although some of the model's assumption are only partially supported by experimental evidence.

Reviewed by eLife