Cell Biology

Endosome-associated Rab GTPases control distinct aspects of neural circuit assembly

1. Katherine X. Dong
2. Hui Ji
3. David J. Luginbuhl
4. Liqun Luo
5. Colleen N. McLaughlin

• Curated by eLife

  eLife Assessment

  Dong et al. present a valuable analysis of mutant phenotypes of the Rab GTPases Rab5, Rab7, and Rab11 in Drosophila second-order olfactory neuron development. This is a solid characterization and comparison of the different Rab mutants on projection neuron development, with clear differences for the three Rabs, and by inference for the early, late, and recycling endosomal functions executed by each.

Reviewed by eLife

CIP2A Mediates the Recruitment of the SLX4-MUS81-XPF Tri-Nuclease Complex in Mitosis and Protects Against Replication Stress

1. Alice Meroni
2. Annica Pellizzari
3. Andrea Hänel
4. Nathalie Varisco
5. Pascale Brasier-Lutz
6. Isabell Witzel
7. Alessandro A Sartori
8. Manuel Stucki

Reviewed by Review Commons

Branched actin polymerization drives invasive protrusion formation to promote myoblast fusion during skeletal muscle regeneration

1. Yue Lu
2. Tezin Walji
3. Pratima Pandey
4. Chuanli Zhou
5. Christa W Habela
6. Scott B Snapper
7. Rong Li
8. Elizabeth H Chen

• Curated by eLife

  eLife Assessment

  This study presents a valuable finding regarding the role of Arp2/3 and the actin nucleators N-WASP and WAVE complexes in myoblast fusion. The data presented is convincing, and the work will be of interest to biologists studying skeletal muscle stem cell biology in the context of skeletal muscle regeneration.

Reviewed by eLife

Analysis of cancer mutations introduced into the Drosophila Notch Negative Regulatory Region uncovers a diversity of regulatory outcomes

1. Hideyuki Shimizu
2. Martin Baron

• Curated by eLife

  eLife Assessment

  This important study presents a technically rigorous and carefully controlled analysis of the signalling potential of cancer-associated gain-of-function Notch alleles. The work is clearly presented, and the experiments are robust, comprehensive, and well-controlled. While some data primarily establish the system or report negative findings, the comparative approach in a well-characterized model provides convincing mechanistic evidence for how these Notch variants function. This study will be of interest to researchers in both developmental and cancer biology.

Reviewed by eLife

A green lifetime biosensor for calcium that remains bright over its full dynamic range

1. Franka H van der Linden
2. Stephen C Thornquist
3. Rick M ter Beek
4. Jelle Y Huijts
5. Mark A Hink
6. Theodorus WJ Gadella
7. Gaby Maimon
8. Joachim Goedhart

• Curated by eLife

  eLife Assessment

  This manuscript reports on an FLIM-based calcium biosensor, G-CaFLITS. It represents an important contribution to the field of genetically-encoded fluorescent biosensors, and will serve as a practical tool for the FLIM imaging community. The paper provides convincing evidence of G-CaFLITS's photophysical properties and its advantages over previous biosensors such as Tq-Ca-FLITS. Although the benefits of G-Ca-FLITS over Tq-Ca-FLITS are limited by the relatively small wavelength shift, it presents some advantages in terms of compatibility with available instrumentation and brightness consistency.

Reviewed by eLife

NudC moonlights in ribosome biogenesis and homeostasis in Drosophila melanogaster polyploid cells

1. Duoduo Shi
2. Yuko Shimada-Niwa
3. Naoki Okamoto
4. Akira Nakamura
5. Yuya Ohhara
6. Wei Sun
7. Ryusuke Niwa

Reviewed by Review Commons

Ciliary and non-ciliary functions of CEP104 in Xenopus

1. Panagiota Louka
2. Louiza Potamiti
3. Mihalis I. Panayiotidis
4. Paris Skourides

Reviewed by Review Commons

A systematic bi-genomic split-GFP assay illuminates the mitochondrial matrix proteome and protein targeting routes

1. Yury S Bykov
2. Solène Zuttion
3. Dunya Edilbi
4. Marina Polozova
5. Johanna Arnold
6. Sergey Malitsky
7. Maxim Itkin
8. Bruno Senger
9. Ofir Klein
10. Yeynit Asraf
11. Hadar Meyer
12. Hubert D Becker
13. Róża Kucharczyk
14. Maya Schuldiner

• Curated by eLife

  eLife Assessment

  This study represents a valuable addition to the catalog of mitochondrial proteins. With the use of methodology based on the bi-genomic split-GFP technology, the authors generate convincing data, including dually localized proteins and topological information, under various growth conditions in yeast. The study represents a key basis for further functional and/or mechanistic studies on mitochondrial protein biogenesis.

Reviewed by eLife

Stress granules and protein aggregates reveal intracellular resource competition

1. Hannah E. Buchholz
2. Sean A. Martin
3. Jane E. Dorweiler
4. Derek C. Prosser
5. Emily M. Sontag
6. Anita L. Manogaran

Reviewed by Arcadia Science

Apelin-13 alleviate inflammatory reaction of ischemia reperfusion in rat kidney transplantation via NF-kappa B signaling pathway

1. Irene Capelli
2. Valeria Aiello
3. Lorenzo Gasperoni
4. Gaetano La Manna

Reviewed by PREreview