Cell Biology

Amoeboid cells undergo durotaxis with soft end polarized NMIIA

1. Chenlu Kang
2. Pengcheng Chen
3. Xin Yi
4. Dong Li
5. Yiping Hu
6. Yihong Yang
7. Huaqing Cai
8. Bo Li
9. Congying Wu

• Curated by eLife

  eLife Assessment

  This study presents an important finding on durotaxis in various amoeboid cells that is independent of focal adhesions. The evidence supporting the authors' claims is compelling. The work will be of interest to cell biologists and biophysicists working on rigidity sensing, the cytoskeleton, and cell migration.

Reviewed by eLife

UGGT1-mediated reglucosylation of N-glycan competes with ER-associated degradation of unstable and misfolded glycoproteins

1. Satoshi Ninagawa
2. Masaki Matsuo
3. Deng Ying
4. Shuichiro Oshita
5. Shinya Aso
6. Kazutoshi Matsushita
7. Mai Taniguchi
8. Akane Fueki
9. Moe Yamashiro
10. Kaoru Sugasawa
11. Shunsuke Saito
12. Koshi Imami
13. Yasuhiko Kizuka
14. Tetsushi Sakuma
15. Takashi Yamamoto
16. Hirokazu Yagi
17. Koichi Kato
18. Kazutoshi Mori

• Curated by eLife

  eLife Assessment

  This important manuscript demonstrates that UGGT1 is involved in preventing the premature degradation of endoplasmic reticulum (ER) glycoproteins through the re-glucosylation of their N-linked glycans following release from the calnexin/calreticulin lectins. The authors include a wealth of convincing data in support of their findings, although extending these findings to other types of substrates, such as secreted proteins, could further demonstrate the global importance of this mechanism for protein trafficking through the secretory pathway. This work will be of interest to scientists interested in ER protein quality control, proteostasis, and protein trafficking.

Reviewed by eLife

MuSK-BMP signaling in adult muscle stem cells maintains quiescence and regulates myofiber size

1. Laura A Madigan
2. Diego Jaime
3. Isabella Chen
4. Justin R Fallon

• Curated by eLife

  eLife Assessment

  This valuable study provides solid support for the participation of the BMP-binding domain of MuSK, a tyrosine kinase mostly known for its role at the neuromuscular junction, in the maintenance and activation of muscle stem cells (SCs). These mononucleated cells, located between the muscle fiber basal lamina and its plasma membrane, are normally quiescent, but following muscle damage, become activated, proliferate, and mediate muscle regeneration. These cells are known to respond to a variety of signaling pathways, but this study makes the case for BMP acting via binding to MuSK in maintaining the quiescent state.

Reviewed by eLife

Constitutively active receptor ADGRA3 signaling induces adipose thermogenesis

1. Zewei Zhao
2. Longyun Hu
3. Bigui Song
4. Tao Jiang
5. Qian Wu
6. Jiejing Lin
7. Xiaoxiao Li
8. Yi Cai
9. Jin Li
10. Bingxiu Qian
11. Siqi Liu
12. Jilu Lang
13. Zhonghan Yang

• Curated by eLife

  eLife Assessment

  The study identifies the adhesion G-protein-coupled receptor A3 (ADGRA3) as a potential target for activating adaptive thermogenesis in white and brown adipose tissue, providing valuable information for scientists in the field of adipose tissue biology and metabolism. Although the authors have addressed some concerns raised by reviewers, the interpretations remain somewhat limited, and the work is deemed incomplete. The evidence supporting ADGRA3's role in thermogenesis is insufficient, necessitating more rigorous experiments to validate the receptor's relevance in adipose tissue. Additionally, the lack of experiments using primary cultures, despite feedback from multiple reviewers, highlights significant shortcomings.

Reviewed by eLife

Periosteal skeletal stem cells can migrate into the bone marrow and support hematopoiesis after injury

1. Tony Marchand
2. Kemi E Akinnola
3. Shoichiro Takeishi
4. Maria Maryanovich
5. Sandra Pinho
6. Julien Saint-Vanne
7. Alexander Birbrair
8. Thierry Lamy
9. Karin Tarte
10. Paul S Frenette
11. Kira Gritsman

• Curated by eLife

  eLife Assessment

  The study is valuable to the field, introducing a new model to test BM-periosteal stem cell function in vivo. The authors' findings suggested that periosteal stem cells are linked to hematopoietic regeneration. More comparisons with the conventional model and direct examination of periosteal stem cell factors in hematopoietic regeneration are missing. The observations are solid, however, the limitations in their experimental model made the overall impact incomplete; there is potential for improvements to be made in this area.

Reviewed by eLife

Quantitative phase imaging with temporal kinetics predicts hematopoietic stem cell diversity

1. Takao Yogo
2. Yuichiro Iwamoto
3. Hans Jiro Becker
4. Takaharu Kimura
5. Ayano Sugiyama-Finnis
6. Tomomasa Yokomizo
7. Toshio Suda
8. Sadao Ota
9. Satoshi Yamazaki

Reviewed by Arcadia Science

The exocyst complex controls multiple events in the pathway of regulated exocytosis

1. Sofía Suárez Freire
2. Sebastián Perez-Pandolfo
3. Sabrina Micaela Fresco
4. Julián Valinoti
5. Eleonora Sorianello
6. Pablo Wappner
7. Mariana Melani

• Curated by eLife

  eLife Assessment

  This study makes an important contribution by characterizing the role of the exocyst in secretory granule exocytosis in the Drosophila larval salivary gland. The results are solid and lead to the novel interpretation that the exocyst participates not only in exocytosis, but also in earlier steps of secretory granule biogenesis and maturation. However, the authors are urged to provide additional proof that the exocyst subunit knockdowns were effective and to acknowledge the possibility that inactivation of an essential exocytosis component could indirectly affect other parts of the secretory pathway.

Reviewed by eLife

The conserved ATPase PCH-2 controls the number and distribution of crossovers by antagonizing crossover formation in C. elegans

1. Bhumil Patel
2. Maryke Grobler
3. Alberto Herrera
4. Elias Logari
5. Valery Ortiz
6. Needhi Bhalla

• Curated by eLife

  eLife Assessment

  This is an important study examining the role of conserved PCH-2 protein at different stages of C. elegans meiosis. The authors use elegant molecular genetic approaches to provide convincing evidence to support their claims. The work will be of interest to scientists studying meiosis, DNA recombination, and chromosome segregation.

Reviewed by eLife

Protein absorption in the zebrafish gut is regulated by interactions between lysosome rich enterocytes and the microbiome

1. Laura Childers
2. Esther Park
3. Siyao Wang
4. Richard Liu
5. Robert Barry
6. Stephen A Watts
7. John F Rawls
8. Michel Bagnat

• Curated by eLife

  eLife Assessment

  In this important study, the authors use zebrafish to examine protein absorption in the gut. Using a combination of imaging and single-cell RNA-seq, they characterize a population of lysosome-rich enterocytes that are essential for protein uptake. They find that the microbiome impacts the ability of these cells to uptake protein. The RNA-seq provides a rich dataset for future functional experiments, which makes a convincing case for the importance of these cells.

Reviewed by eLife

Multi-color fluorescence live-cell imaging in Dictyostelium discoideum

1. Hidenori Hashimura
2. Satoshi Kuwana
3. Hibiki Nakagwa
4. Kenichi Abe
5. Tomoko Adachi
6. Toyoko Sugita
7. Shoko Fujishiro
8. Gen Honda
9. Satoshi Sawai

Reviewed by Arcadia Science