Cell Biology

Specific GPCRs Elicit Unique Extracellular Vesicle MiRNA Array Signatures: An Exploratory Study

1. Xiao Shi
2. Michelle C Palumbo
3. Sheila Benware
4. Jack Wiedrick
5. Sheila Markwardt
6. Aaron Janowsky

• Curated by eLife

  eLife Assessment

  This study presents valuable findings by demonstrating that specific GPCR subtypes induce distinct extracellular vesicle miRNA signatures, highlighting a potential novel mechanism for intercellular communication with implications for receptor pharmacology within the field. The data is compelling, however, more evidence is needed to determine whether the distinct extracellular vesicle miRNA signatures result from GPCR-dependent miRNA expression or GPCR-dependent incorporation of miRNAs into extracellular vesicles.

Reviewed by eLife

RNA triggers chronic stress during neuronal aging

1. Kevin Rhine
2. Elle Epstein
3. Natasha M. Carlson
4. Xuezhen Ge
5. Orel Mizrahi
6. Anika Kamat
7. Anita Hermann
8. William R. Brothers
9. John Ravits
10. Eric J. Bennett
11. Gülçin Pekkurnaz
12. Gene W. Yeo

Reviewed by PREreview

Microscopy Nodes: versatile 3D microscopy visualization with Blender

1. Oane Gros
2. Chandni Bhickta
3. Granita Lokaj
4. Yannick Schwab
5. Simone Köhler
6. Niccolò Banterle

Reviewed by Review Commons

Enalapril mitigates senescence and aging-related phenotypes in human cells and mice via pSmad1/5/9-driven antioxidative genes

1. Wencong Lyu
2. Haochen Wang
3. Zhehao Du
4. Ran Wei
5. Jianuo He
6. Fanju Meng
7. Jinlong Bi
8. Lijun Zhang
9. Chao Zhang
10. Yiting Guan
11. Wei Tao

• Curated by eLife

  eLife Assessment

  This study provides valuable insights into the anti-senescence effects of enalapril, identifying pSmad1/5/9 signaling and associated antioxidant pathways as key mediators of its physiological benefits in aged mice. The authors present solid experimental evidence across both in vitro and in vivo systems, demonstrating improved organ function and reduced senescence markers following treatment. Overall, the work supports the repurposing potential of enalapril in aging research and expands understanding of its molecular targets.

Reviewed by eLife

Testosterone-Induced Metabolic Changes in Seminal Vesicle Epithelium Modify Seminal Plasma Components with Potential to Improve Sperm Motility

1. Takahiro Yamanaka
2. Zimo Xiao
3. Natsumi Tsujita
4. Mahmoud Awad
5. Takashi Umehara
6. Masayuki Shimada

• Curated by eLife

  eLife Assessment

  This important work elucidates the biological processes and detailed mechanisms by which testosterone influences seminal plasma metabolites in mice. The evidence supporting the upregulation of metabolic enzymes and the role of ACLY is solid, highlighting the potential contributions of fatty acids to sperm motility.

Reviewed by eLife

Organization of the Yeast Seipin Complex Reveals Differential Recruitment of Regulatory Proteins

1. Yoel A. Klug
2. Pedro Carvalho

Reviewed by Review Commons

Efficiency and localisation of AURKA degradation by PROTACs is modulated by deubiquitinases UCHL5 and target-selective OTUD6A

1. Annabel Cardno
2. Karen Roberts
3. Catherine Lindon

• Curated by eLife

  eLife Assessment

  This study describes a genetic screen to identify deubiquitinases (DUBs) that counteract the activity of small-molecule degraders (PROTACs). The presented data are valuable, identifying OTUD6A and UCHL5 as DUBs that impact the efficacy and potency of PROTACs. While the conclusions are broadly supported and the methods employed are solid, the mechanistic depth and validation are incomplete. Overall, these findings merit further evaluation by the targeted protein degradation community when developing and optimizing PROTACs.

Reviewed by eLife

The Anti-Inflammatory Role of GPNMB in Post-Traumatic Osteoarthritis

1. Asaad A Al-Adlaan
2. Bryson Cook
3. Solorzano Z Ernesto
4. Nazar J Hussein
5. Fatima A Jaber
6. Trinity Kronk
7. Salvatore Frangiamore
8. Hope C Ball
9. Fayez F Safadi

• Curated by eLife

  eLife Assessment

  This study offers useful findings demonstrating the cartilage-protective effects of osteoactivin in inflammatory experimental models. The study provides compelling evidence that osteoactivin may serve as a promising therapeutic target for inflammatory joint diseases.

Reviewed by eLife

The insulin / IGF axis is critically important controlling gene transcription in the podocyte

1. Jennifer A Hurcombe
2. Lusyan Dayalan
3. Fern Barrington
4. Frédéric Burdet
5. Lan Ni
6. Joseph T Coward
7. Paul T Brinkkoetter
8. Martin Holzenberger
9. Aaron Jeffries
10. Sebastian Oltean
11. Gavin I Welsh
12. Richard JM Coward

• Curated by eLife

  eLife Assessment

  This study investigated the role of insulin receptor (IR) and insulin-like growth factor 1 receptor (IGF1R) in the renal glomerular podocytes by characterizing the mice with dual deletion of both receptors in vivo as well as the cultured murine podocytes with induced deletion of both receptors in vitro. The solid data presented in this paper demonstrated the critical requirement of both IR and IGF1R signaling in normal podocyte physiology in mice, albeit a more detailed characterization of the mouse model is desired. Interestingly, long-range sequencing revealed significant retention of introns in mRNAs, due to an altered spliceosome level resulted from the loss of IR and IGF1 signaling in cultured podocytes. This new finding suggests an essential role of IR and IGF1R signaling in regulating RNA metabolism in podocyte, which provides useful information for the understanding of physiology and metabolism of podocytes. However, the underlying molecular mechanism for such a regulation is still unclear and awaits further studies.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife

A biochemical mechanism for Stu2/XMAP215-family microtubule polymerases

1. Binnu Gangadharan
2. Daniel L Kober
3. Luke M Rice

• Curated by eLife

  eLife Assessment

  In their important manuscript, Gangadharan, Kober and Rice focus on how Stu2/XMAP215-family microtubule polymerases use their TOG domains to catalytically promote microtubule growth, testing whether their mechanism follows an enzyme-like kinetic model similar to that of actin polymerases. The authors integrate measurements including microtubule polymerization rates and TOG-tubulin binding kinetics to convincingly show that Stu2 follows an enzyme-like model where tight tubulin binding enables efficient polymerization, revealing a shared mechanism with actin polymerases despite their evolutionary divergence. This work will be of general interest to the cell biology and biophysics communities.

Reviewed by eLife