Cell Biology

Morphological Reprogramming of Primary Cilia Length Mitigates the Fibrotic Phenotype in Fibroblasts Across Diverse Fibrotic Conditions

1. Priyanka Verma
2. Bharat Yalavarthi
3. Swati Bhattacharyya
4. Dinesh Khanna
5. Johann E. Gudjonsson
6. Lam C. Tsoi
7. Rebecca Wells
8. Rebecca L Ross
9. Natalia Riobo-Del Galdo
10. Francesco Del Galdo
11. Sean M. Fortier
12. Maria E. Teves
13. John Varga
14. Dibyendu Bhattacharyya

Reviewed by Review Commons

Therapeutic effects of PDGF-AB/BB against cellular senescence in human intervertebral disc

1. Changli Zhang
2. Martha Elena Diaz-Hernandez
3. Takanori Fukunaga
4. Shenoy Sreekala
5. Sangwook Tim Yoon
6. Lisbet Haglund
7. Hicham Drissi

• Curated by eLife

  eLife Assessment

  This work demonstrates the therapeutic potential of recombinant human PDGF-AB/BB proteins in alleviating the senescent signatures of primary human intervertebral disc cells. The study represents a fundamental, significant advance in the treatment of intervertebral disc degeneration through the suppression of senescence. The strength of evidence supporting these conclusions is compelling, as it is primarily based on transcriptomic analysis and direct protein measurements from relatively homogeneous cell populations. This work will be of interest to spine basic scientists and clinicians, as well as to musculoskeletal scientists more broadly. The revised manuscript adds greater clarity, and the impact of the study is greatly enhanced.

Reviewed by eLife

JAK-STAT pathway activation compromises nephrocyte function in a Drosophila high-fat diet model of chronic kidney disease

1. Yunpo Zhao
2. Jianli Duan
3. Hannah Seah
4. Joyce van de Leemput
5. Zhe Han

• Curated by eLife

  eLife Assessment

  This study presents important new insights linking obesity to kidney disease using a Drosophila model. A series of compelling experiments demonstrate that a high-fat diet induces excretion of a leptin-like JAK-STAT ligand from fat body, driving the adipose-nephrocyte axis through activated JAK-STAT signaling and subsequently causing a functional defect in nephrocytes. The approach using combination of genetic tools and pharmacological intervention is solid and confirms the mechanistic link, together with phenotypic analysis that further supports the authors conclusions.

Reviewed by eLife

Melanocyte differentiation and mechanosensation are differentially modulated by distinct extracellular matrix proteins

1. Carole Luthold
2. Marie Didion
3. Vanessa Samira Rácz
4. Emilio Benedum
5. Ann-Kathrin Burkhart
6. Nina Demmerle
7. Evelyn Wirth
8. Gubesh Gunaratnam
9. Sudharshini Thangamurugan
10. Volkhard Helms
11. Markus Bischoff
12. Annika Ridzal
13. Sandra Iden

Reviewed by Review Commons

Uev1A counteracts oncogenic Ras stimuli in both polyploid and diploid cells

1. Qi Zhang
2. Yunfeng Wang
3. Xueli Fu
4. Ziguang Wang
5. Yang Zhang
6. Lizhong Yan
7. Yuejia Wang
8. Muhan Yang
9. Dongze Song
10. Ruixing Zhang
11. Hongru Zhang
12. Shian Wu
13. Shaowei Zhao

• Curated by eLife

  eLife assessment

  This valuable study examines the role of E2 ubiquitin enzyme, Uev1a in tissue resistance to oncogenic RasV12 in Drosophila melanogaster polyploid germline cells and human cancer cell lines. The incomplete evidence suggests that Uev1a works with the E3 ligase APC/C to degrade Cyclin A, and the strength of evidence could be increased by addressing the expression of CycA in the ovaries and the uev1a loss of function in human cancer cells. This work would be of interest to researchers in germline biology and cancer.

Reviewed by eLife

Sphingolipid imbalance aggravates tau pathology by endomembrane rigidification and rupture

1. Jessica Tittelmeier
2. Carl Alexander Sandhof
3. Nicole Martin
4. Deike El-Kabarity
5. Soki-Bradel Ngonza-Nito
6. Ronald Melki
7. Carmen Nussbaum-Krammer

• Curated by eLife

  eLife Assessment

  This valuable study addresses the role of sphingolipid metabolism in maintaining endolysosomal membrane integrity and its impact on tau pathology in Caenorhabditis elegans and human cell culture models. The methods are solid and the proposed mechanisms are conceivable. However, the current evidence is incomplete and could be strengthened, due to reliance on imaging data and insufficient biochemical validation. The work will be of broad interest to cell biologists and biologists working on Alzheimer's disease and related proteinopathies.

Reviewed by eLife

Intra-manchette transport employs both microtubule and actin tracks

1. Jo H. Judernatz
2. Laura Pérez Pañeda
3. Tereza Kadavá
4. Albert J. R. Heck
5. Tzviya Zeev-Ben-Mordehai

Reviewed by Review Commons

Succinate Dehydrogenase loss causes cascading metabolic effects that impair pyrimidine biosynthesis

1. Madeleine L. Hart
2. Kristian Davidsen
3. Serwah Danquah
4. Eric Zheng
5. David Sokolov
6. Lucas B. Sullivan

Reviewed by preLights

GMCL1 Controls 53BP1 Stability and Modulates Paclitaxel Sensitivity in Cancer

1. Yuki Kito
2. Tania J González-Robles
3. Sharon Kaisari
4. Juhee Pae
5. Sheena Faye Garcia
6. Juliana Ortiz-Pacheco
7. Beatrix Ueberheide
8. Ruth Lehmann
9. Antonio Marzio
10. Gergely Rona
11. Michele Pagano

• Curated by eLife

  eLife Assessment

  This study identifies 53BP1 as an interaction partner of GMCL1 (a likely CUL3 substrate receptor). The study seeks to link this finding to regulation of the mitotic surveillance pathway and paclitaxel resistance in cancer. The evidence for these claims is currently inadequate; concerns include the use of cell lines that have been reported to lack the mitotic surveillance pathway, insufficient consideration of paclitaxel mechanisms of action, and an overinterpretation of correlative results.

Reviewed by eLife

Extracellular vesicle-mediated release of bis(monoacylglycerol)phosphate is regulated by LRRK2 and Glucocerebrosidase activity

1. Elsa Meneses-Salas
2. Marianna Arnold
3. Moises Castellá
4. Frank Hsieh
5. Ruben Fernández-Santiago
6. Mario Ezquerra
7. Alicia Garrido
8. María-José Martí
9. Carlos Enric
10. Suzanne R Pfeffer
11. Kalpana Merchant
12. Albert Lu

• Curated by eLife

  eLife Assessment

  This useful study presents the potentially interesting concept that LRRK2 regulates cellular BMP levels and their release via extracellular vesicles, with GCase activity further modulating this process in mutant LRRK2-expressing cells. However, the evidence supporting the conclusions remains incomplete, and certain statistical analyses are inadequate. This work would be of interest to cell biologists working on Parkinson's disease.

Reviewed by eLife