Cell Biology

Mutations that prevent phosphorylation of the BMP4 prodomain impair proteolytic maturation of homodimers leading to lethality in mice

1. Hyung-seok Kim
2. Mary L Sanchez
3. Joshua Silva
4. Heidi L Schubert
5. Rebecca Dennis
6. Christopher P Hill
7. Jan L Christian

• Curated by eLife

  eLife Assessment

  This fundamental work presents two clinically relevant BMP4 mutations that contribute to vertebrate development. The compelling evidence, both from wet lab and AI generated predictions, supports that the site-specific cleavage at the BMP4 pro-domain precisely regulates its function and provides mechanistic insight how homodimers and heterodimers behave differently. The work will be of board interest to researchers working on growth factor signaling mechanisms and vertebrate development.

Reviewed by eLife

Optogenetic and chemical genetic tools for rapid repositioning of vimentin intermediate filaments

1. Milena Pasolli
2. Joyce C. M. Meiring
3. James P. Conboy
4. Gijsje H. Koenderink
5. Anna Akhmanova

Reviewed by Review Commons

CellPhePy: a Python implementation of the CellPhe toolkit for automated cell phenotyping from microscopy time-lapse videos

1. Laura Wiggins
2. Stuart Lacy
3. Graeme Park
4. Joanne Marrison
5. Ben Powell
6. Beth Cimini
7. Peter O’Toole
8. Julie Wilson
9. William J. Brackenbury

Reviewed by Arcadia Science

IFT20 regulates lymphatic endothelial cell-cell junctions via endocytic trafficking of VE-cadherin

1. Delayna Paulson
2. Ahana Majumder
3. Zachary Lehmann
4. Luke Knutson
5. Jacob Paulson
6. Shannon Lasey
7. Darci M. Fink

Reviewed by Review Commons

Cell cycle-regulated transcriptional pausing of Drosophila replication-dependent histone genes

1. James P. Kemp
2. Mark S. Geisler
3. Mia Hoover
4. Chun-Yi Cho
5. Patrick H. O’Farrell
6. William F. Marzluff
7. Robert J. Duronio

Reviewed by Review Commons

Treacle’s ability to form liquid-like phase condensates is essential for nucleolar fibrillar center assembly, efficient rRNA transcription and processing, and rRNA gene repair

1. Artem K Velichko
2. Nadezhda V Petrova
3. Dmitry A Deriglazov
4. Anastasia P Kovina
5. Artem V Luzhin
6. Eugene P Kazakov
7. Igor I Kireev
8. Sergey Razin
9. Omar L Kantidze

• Curated by eLife

  eLife Assessment

  This important study reveals that the nucleolar protein Treacle undergoes liquid-liquid phase separation in vitro and in vivo. It provides convincing evidence that the ability of Treacle to form phase-separated condensates is necessary for the proper formation of the fibrillar center of the nucleolus, rRNA transcription, and rDNA repair. These findings will be of interest to the communities studying biomolecular condensates, nucleolar organization, and ribosome biogenesis.

Reviewed by eLife

Teach your microscope how to print: Low-cost and rapid-iteration microfabrication for biology

1. Lucien Hinderling
2. Remo Hadorn
3. Moritz Kwasny
4. Joël Frei
5. Benjamin Grädel
6. Sacha Psalmon
7. Yannick Blum
8. Rémi Berthoz
9. Alex Landolt
10. Benjamin D. Towbin
11. Daniel Riveline
12. Olivier Pertz

Reviewed by Arcadia Science

Membrane microdomains are crucial for Mycobacterium marinum EsxA-dependent membrane damage, escape to the cytosol and infection

1. Cristina Bosmani
2. Angélique Perret
3. Florence Leuba
4. Aurélie Guého
5. Nabil Hanna
6. Thierry Soldati

Reviewed by Review Commons

Intestinal fibroblast heterogeneity: unifying RNA-seq studies and introducing consensus-driven nomenclature

1. Neda Glisovic
2. Aleksandra Chikina
3. Noémie Robil
4. Sonia Lameiras
5. Danijela Matic Vignjevic

Reviewed by Review Commons

C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin

1. Jessica Mella
2. Regan Volk
3. Balyn Zaro
4. Abigail Buchwalter

• Curated by eLife

  eLife Assessment

  This solid work, a Research Advance linked to Buchwalter et al., 2019, demonstrates that epitope tagging influences protein fate, serving as a cautionary example of how different tagging and imaging strategies may alter the pattern of endogenous protein trafficking. The information presented will be useful for researchers in the field of membrane trafficking, particularly in guiding their experimental designs. That being said, the study offers limited new insights into the biogenesis or disposal of endogenous Emerin.

Reviewed by eLife