Cell Biology

Novel Nuclear Roles for Testis-Specific ACTL7A and ACTL7B Supported by In Vivo Characterizations and AI Facilitated In Silico Mechanistic Modeling with Implications for Epigenetic Regulation in Spermiogenesis

1. Pierre Ferrer
2. Srijana Upadhyay
3. James J. Cai
4. Tracy M. Clement

• Curated by eLife

  eLife assessment

  This valuable study reports that actin-related proteins may be involved in transcriptional regulation during spermatogenesis. The supporting data remain incomplete, and more extensive disentanglement from the canonical role of these actin-related proteins and the experimental validation of in silico predictions are required. This work will be of interest to reproductive biologists and other researchers working on non-canonical roles of actin and actin-related proteins.

Reviewed by eLife

Spatiotemporal recruitment of the ubiquitin-specific protease USP8 directs endosome maturation

1. Yue Miao
2. Yongtao Du
3. Baolei Wang
4. Jingjing Liang
5. Yu Liang
6. Song Dang
7. Jiahao Liu
8. Dong Li
9. Kangmin He
10. Mei Ding

• Curated by eLife

  eLife Assessment

  The manuscript presents an important model for the field of endosome maturation, providing perspective on the role of the deubiquitinating enzyme UPS-50/USP8 in the process. The evidence presented in the paper is clear, incorporating well-designed experiments that suggest the dual actions of UPS-50 and USP8 in the conversion of early endosomes into late endosomes. Overall, the work is convincing and centers on an intriguing subject.

Reviewed by eLife

ScRNA-seq and scATAC-seq reveal that sertoli cell mediate spermatogenesis disorders through stage-specific communications in non-obstructive azoospermia

1. Shimin Wang
2. Hongxian Wang
3. Bicheng Jin
4. Hongli Yan
5. Qingliang Zheng
6. Dong Zhao

• Curated by eLife

  eLife assessment

  This study provides valuable scRNA-seq and scATAC-seq data for testicular tissues from patients with spermatogenesis disorders. By examining the transcriptomic and epigenetic changes in Sertoli cells, the authors uncovered key regulatory mechanisms underlying male infertility and identified potential therapeutic targets. While some of the cellular profiling results are convincing, the analyses for differential profiling of NOA cases and epigenomics data remain incomplete in their current form.

Reviewed by eLife

Temporally controlled nervous system-to-gut signaling bidirectionally regulates longevity in C. elegans

1. Lingxiu Xu
2. Chengxuan Han
3. Lei Chun
4. X.Z. Shawn Xu
5. Jianfeng Liu

• Curated by eLife

  eLife assessment

  This study reports that the timing of 'brain-to-gut' signaling influences the lifespan of the C. elegans model. The main finding, that modulating the same neurotransmitter, Acetylcholine, at different ages elicits lifespan shortening - or extending - effects utilizing different receptors, is important and of broad interest to the longevity field as recognized by all the reviewers. The data is largely consistent with the authors' model, but the strength of the evidence is incomplete. The study requires several rigorous experiments detailed by the reviewers to substantiate the main conclusions.

Reviewed by eLife

O-GlcNAcylation of the intellectual disability protein DDX3X exerts proteostatic cell cycle control

1. Conor W. Mitchell
2. Huijie Yuan
3. Marie Sønderstrup-Jensen
4. Florence Authier
5. Alfonso Manuel D’Alessio
6. Andrew T. Ferenbach
7. Daan M.F. van Aalten

Reviewed by Review Commons

Mir221/222 drive synovial hyperplasia and arthritis by targeting cell cycle inhibitors and chromatin remodeling components

1. Fani Roumelioti
2. Christos Tzaferis
3. Dimitris Konstantopoulos
4. Dimitra Papadopoulou
5. Alejandro Prados
6. Maria Sakkou
7. Anastasios Liakos
8. Panagiotis Chouvardas
9. Theodore Meletakos
10. Yiannis Pandis
11. Niki Karagianni
12. Maria C Denis
13. Maria Fousteri
14. Maria Armaka
15. George Kollias

• Curated by eLife

  eLife assessment

  This important manuscript used state-of-the-art techniques and employed relevant animal models to provide both convincing and solid evidence supporting the regulatory role of microRNA cluster 221/222 in rheumatoid arthritis synovial fibroblast. The findings of this work offer significant advances to current knowledge which will be interesting to a wide range audience in the rheumatology and bone research fields. However, whereas models, techniques, and analyses are solid, certain concepts related to the role of immune and bone cells are limited.

Reviewed by eLife

Nucleocytoplasmic transport senses mechanics independently of cell density in cell monolayers

1. Ignasi Granero-Moya
2. Guillaume Belthier
3. Bart Groenen
4. Marc Molina-Jordán
5. Miguel González-Martín
6. Xavier Trepat
7. Jacco van Rheenen
8. Ion Andreu
9. Pere Roca-Cusachs

Reviewed by Review Commons

Dynamic Tracking of Native Precursors in Adult Mice

1. Suying Liu
2. Sarah E. Adams
3. Haotian Zheng
4. Juliana Ehnot
5. Seul K. Jung
6. Greer Jeffrey
7. Theresa Menna
8. Louise E. Purton
9. Hongzhe Lee
10. Peter Kurre

• Curated by eLife

  eLife assessment

  This important study describes a new mathematical method to analyze clonal composition of tissues using fluorescent reporters and to estimate the number of precursor cells contributing to tissue homeostasis and regeneration based on statistical variance. The evidence provided is convincing, with rigorous measurement of hematopoietic cell labeling during steady state and regenerative hematopoiesis following insult. It could be further strengthened by exploring the limitations of the binomial assumption, using tools to measure clonality and considering the possible effects of the inducing agent (tamoxifen) on precursor cells. The manuscript not only presents a compelling approach to better understand tissue dynamics, it also challenges some ideas in pathological hematopoiesis, opens new research directions and is thus of broad interest to stem cell and developmental biologists.

Reviewed by eLife

G6b-B antibody-based cis-acting platelet receptor inhibitors (CAPRIs) as a new family of anti-thrombotic therapeutics

1. Alexandra Mazharian
2. Ophélie Bertin
3. Amrita Sarkar
4. Johanna Augros
5. Alicia Bornert
6. Cécile Loubière
7. Friederike Jönsson
8. Juli Warwicker
9. W Mark Abbott
10. Omer Dushek
11. Caroline Vayne
12. Lubica Rauova
13. Klaus Fütterer
14. Jérôme Rollin
15. Mortimer Poncz
16. Yotis A Senis

Reviewed by preLights

Alstrom syndrome proteins regulate centriolar cartwheel assembly by enabling Plk4-Ana2 amplification loop in Drosophila

1. Marine Brunet
2. Joelle Thomas
3. Jean-Andre Lapart
4. Léo Krüttli
5. Marine Laporte
6. Maria Giovanna Riparbelli
7. Giuliano Callaini
8. Benedicte Durand
9. Veronique Morel

Reviewed by Review Commons