Cell Biology

MCL1 may not mediate chemoresistance

1. Kylin A. Emhoff
2. Kunho Chung
3. Dongmei Zhang
4. Belinda Willard
5. Timothy Chan
6. Babal Kant Jha
7. Shaun R. Stauffer
8. Jesse A. Coker
9. Jan Joseph Melenhorst

Reviewed by preLights

Multiciliated cells adapt the mechanochemical Piezo1-Erk1/2-Yap1 cell proliferation axis to fine-tune centriole number

1. Vani Narayanan
2. Venkatramanan G Rao
3. Angelo Arrigo
4. Saurabh S Kulkarni

Reviewed by Review Commons

Septins function in exocytosis via physical interactions with the exocyst complex in fission yeast cytokinesis

1. Davinder Singh
2. Yajun Liu
3. Yi-Hua Zhu
4. Sha Zhang
5. Shelby M Naegele
6. Jian-Qiu Wu

• Curated by eLife

  eLife Assessment

  How secretion is regulated during cell division and how membrane trafficking factors cooperate with the cytoskeleton during cell division remain poorly understood. In this work the authors find protein-protein interactions and localization dependencies between the polymeric septin cytoskeleton and the exocyst complex, using fission yeast as a model organism and using alphafold 3 based structural predictions. The work provides a valuable body of new information that will be of great interest to the cell biology community. The evidence is solid and provides the authors and the community a framework to test if the identified interfaces reflect bona fide interaction sites in vivo and in vitro in future.

Reviewed by eLife

High-content high-resolution microscopy and deep learning-assisted analysis reveals host and bacterial heterogeneity during Shigella infection

1. Ana Teresa López-Jiménez
2. Dominik Brokatzky
3. Kamla Pillay
4. Tyrese Williams
5. Gizem Özbaykal Güler
6. Serge Mostowy

• Curated by eLife

  eLife Assessment

  This manuscript describes an AI-automated microscopy-based approach to characterize both bacterial and host cell responses associated with Shigella infection of epithelial cells. The methodology is compelling and should be helpful for investigators studying a variety of intracellular pathogens. The authors have acquired important findings regarding host and bacterial responses in the context of infection, which should be followed up with further mechanistic-based studies.

Reviewed by eLife

A tool to pulse-label yeast Nuclear Pore Complexes in imaging and biochemical experiments

1. Annemiek C Veldsink
2. Jonas S Fischer
3. Sophie Hell
4. Karsten Weis
5. Liesbeth M Veenhoff

• Curated by eLife

  eLife Assessment

  This valuable study introduces a non-perturbative pulse-labeling strategy for yeast nuclear pore complexes (NPCs), employing a nanobody-based approach in order to selectively capture Nup84-containing complexes for imaging and biochemical analysis. The data convincingly demonstrate that a short induction period (20 minutes to 1 hour) yields a strong and sustained signal, enabling affinity purification that faithfully recapitulates the endogenous Nup84 interactome. This tool offers a powerful framework for investigating NPC dynamics and associated interactomes through both imaging and biochemical assays.

Reviewed by eLife

Codon-specific ribosome stalling reshapes translational dynamics during branched-chain amino acid starvation

1. Lina Worpenberg
2. Cédric Gobet
3. Felix Naef

Reviewed by Review Commons

Insulin-like peptide secretion is mediated by peroxisome-Golgi interplay

1. Marie A. König
2. Nicole Kucharowski
3. Darla P. Dancourt Ramos
4. Hannah Soyka
5. Klaus Wunderling
6. Torsten R. Bülow
7. Mohamed H. Yaghmour
8. Christoph Thiele
9. Jan M. Ache
10. Lars Kuerschner
11. Margret H. Bülow

Reviewed by PREreview

Xrp1 drives damage-induced cellular plasticity of enteroendocrine cells in the adult Drosophila midgut

1. Qingyin Qian
2. Hiroki Nagai
3. Yuya Sanaki
4. Makoto Hayashi
5. Kenichi Kimura
6. Yu-ichiro Nakajima
7. Ryusuke Niwa

Reviewed by Review Commons

Matrix-associated extracellular vesicles modulate human smooth muscle cell adhesion and directionality by presenting collagen VI

1. Alexander N Kapustin
2. Sofia Serena Tsakali
3. Meredith Whitehead
4. George Chennell
5. Meng-Ying Wu
6. Chris Molenaar
7. Anton Kutikhin
8. Yimeng Chen
9. Sadia Ahmad
10. Leo Bogdanov
11. Maxim Sinitsky
12. Kseniya Rubina
13. Aled Clayton
14. Frederik J Verweij
15. Dirk Michiel Pegtel
16. Simona Zingaro
17. Arseniy Lobov
18. Bozhana Zainullina
19. Dylan Owen
20. Maddy Parsons
21. Richard E Cheney
22. Derek T Warren
23. Martin James Humphries
24. Thomas Iskratsch
25. Mark Holt
26. Catherine M Shanahan

• Curated by eLife

  eLife Assessment

  This paper explores the role of extracellular vesicles in providing extracellular matrix signals for migration of vascular smooth muscle cells. The evidence, based on cell culture experiments and supporting imaging of human samples, is mostly convincing. The paper will be valuable for researchers investigating cell migration during vessel repair and atherogenesis.

Reviewed by eLife

Ubiquitination-activated TAB–TAK1–IKK–NF-κB axis modulates gene expression for cell survival in the lysosomal damage response

1. Akinori Endo
2. Chikage Takahashi
3. Naoko Ishibashi
4. Yasumasa Nishito
5. Koji Yamano
6. Keiji Tanaka
7. Yukiko Yoshida

• Curated by eLife

  eLife Assessment

  This study presents the important finding that lysosomal damage triggers inflammatory signaling through ubiquitination and the TAB-TAK1-IKK-NF-kB axis. The data obtained from the unbiased transcriptomic and proteomic analyses are convincing and provide invaluable information to the field. Although further experiments will be required to clarify how TAB2/3 are recruited after various types of lysosome damage, this work will be of interest to researchers in the fields of organelle biology and inflammation.

Reviewed by eLife