Cell Biology

Mycobacterium tuberculosis -infected macrophages accumulate more nanoparticles

1. Amine Pochet
2. Tom Bourguignon
3. Axelle Grandé
4. Jesus Alfredo Godinez-Leon
5. Joan Fine
6. Priscille Brodin
7. Arnaud Machelart
8. Ruxandra Gref

Reviewed by Rapid Reviews Infectious Diseases

The microcephaly protein WDR62 regulates cellular purine metabolism through the HSP70/HSP90 chaperone machinery

1. Matthew J. Morris
2. Yvonne Y. Yeap
3. Chi Chen
4. S. Sean Millard
5. Julia K. Pagan
6. Dominic C. H. Ng

Reviewed by Review Commons

The Arp2/3 complex maintains genome integrity and survival of epidermal Langerhans cells

1. Maria-Graciela Delgado
2. Ann-Kathrin Burkhart
3. Javiera Villar
4. Roberto Amadio
5. Vanessa S. Racz
6. Doriane Sanséau
7. Nilushi De Silva
8. Livia Lacerda Mariano
9. Anna Shmakova
10. Anna Chipont
11. Mabel San Roman
12. Giulia Bracchetti
13. Vincent Calmettes
14. Zahraa Alraies
15. Mathieu Maurin
16. Aurelien Dauphin
17. Coralie Guerin
18. Florent Ginhoux
19. Nicolas Manel
20. Daniele Fachinetti
21. Federica Benvenuti
22. Sandra Iden
23. Ana-Maria Lennon-Duménil

Reviewed by Review Commons

Identification of Wnt regulated genes that are repressed by, or independent of, β-catenin

1. Shiyang Liu
2. Sara Haghani
3. Enrico Petretto
4. Babita Madan
5. Nathan Harmston
6. David M Virshup

Reviewed by Review Commons

Engineered migrasomes provide a robust and thermally stable vaccination platform

1. Dongju Wang
2. Haifang Wang
3. Wei Wan
4. Zihui Zhu
5. Takami Sho
6. Yi Zheng
7. Xing Zhang
8. Longyu Dou
9. Qiang Ding
10. Li Yu
11. Zhihua Liu

• Curated by eLife

  eLife Assessment

  This study, from the group that pioneered migrasome, describes a novel vaccine platform of engineered migrosomes that behave like natural migrasomes. Importantly, this platform has the potential to overcome obstacles associated with cold chain issues for vaccines such as mRNA. In the revised version, the authors have addressed previous concerns and the results from additional experiments provide compelling evidence that features methods, data, and analyses more rigorous than the current state-of-the-art. Although the findings are important with practical implications for the vaccine technology, results from additional experiments would make this an outstanding study.

Reviewed by eLife

Hexokinase regulates Mondo-mediated longevity via the PPP and organellar dynamics

1. Raymond Laboy
2. Marjana Ndoci
3. Shamsh Tabrez Syed
4. Maximilian Vonolfen
5. Eugen Ballhysa
6. Tim Droth
7. Klara Schilling
8. Anna Loehrke
9. Ilian Atanassov
10. Adam Antebi

• Curated by eLife

  eLife Assessment

  This important study utilizes the nematode C. elegans and mammalian cell culture to investigate the role of MML-1/Mondo in conserved regulation of metabolism and aging. The evidence supporting the conclusions is convincing and covers a range of areas including localization, upstream pathways, and conservation. The paper will be of interest to a broad range of biologists studying aging, metabolism, and transcriptional regulation.

Reviewed by eLife

Forward genetics in C. elegans reveals genetic adaptations to polyunsaturated fatty acid deficiency

1. Delaney Kaper
2. Uroš Radović
3. Per-Olof Bergh
4. August Qvist
5. Marcus Henricsson
6. Jan Borén
7. Marc Pilon

• Curated by eLife

  eLife Assessment

  This fundamental study investigates the role of polyunsaturated fatty acids (PUFAs) in physiology and membrane biology, using a unique model to perform a thorough genetic screen that demonstrates that PUFA synthesis defects cannot be compensated for by mutations in other pathways. These findings are supported by compelling evidence from a high quality genetic screen, functional validation of their hits, and lipid analyses. This study will appeal to researchers in membrane biology, lipid metabolism, and C. elegans genetics.

Reviewed by eLife

Rho-ROCK liberates sequestered claudin for rapid de novo tight junction formation

1. Yuma Cho
2. Akari Taniguchi
3. Akiharu Kubo
4. Junichi Ikenouchi

• Curated by eLife

  eLife Assessment

  This paper identifies a crucial step in the regulation of tight junction formation by identifying Rho-ROCK activity-dependent activation of the serine protease Matriptase, making Claudins available for tight junction formation. The reviewers were satisfied with the revisions and found the work important and the approach convincing.

Reviewed by eLife

Targeting the WSB2–NOXA axis in cancer cells for enhanced sensitivity to BCL-2 family protein inhibitors

1. Dongyue Jiao
2. Kun Chang
3. Jiamin Jin
4. Yingji Chen
5. Mo Ren
6. Yucong Zhang
7. Kun Gao
8. Yaoting Xu
9. Lixin Wang
10. Chenji Wang

• Curated by eLife

  eLife Assessment

  This study reports a fundamental observation concerning cell death regulation by the anti-apoptotic BCL2 family NOXA. The authors convincingly demonstrate that NOXA is destabilized through the interaction with WSB2, a substrate receptor in CRL5 ubiquitin ligase complex, sensitizing the cells to treatments. These are key findings for cell biologists and cancer researchers as they identified a new target impacting drug responsiveness in cancer therapies.

Reviewed by eLife

Human formin FHOD3-mediated actin elongation is required for sarcomere integrity in cardiomyocytes

1. Dylan A Valencia
2. Angela N Koeberlein
3. Haruko Nakano
4. Akos Rudas
5. Aanand A Patel
6. Airi Harui
7. Cassandra Spencer
8. Atsushi Nakano
9. Margot E Quinlan

• Curated by eLife

  eLife Assessment

  Valencia et al. combine elegant in vitro biochemical experiments with functional assays in cardiomyocytes to determine which properties of the FHOD3 formin are essential for sarcomere assembly. Using separation-of-function mutants, they show that FHOD3's elongation activity, rather than its nucleation, capping, or bundling activities, is key to its sarcomeric function. This is an important finding and the data presented in the manuscript are convincing; however, the presence of FHOD3 at filament barbed ends in the TIRF elongation assays should probably be verified directly in a future study.

Reviewed by eLife