Cell Biology

Single-nucleus transcriptomics reveal the cytological mechanism of conjugated linoleic acids in regulating intramuscular fat deposition

1. Liyi Wang
2. Shiqi Liu
3. Shu Zhang
4. Yizhen Wang
5. Yanbing Zhou
6. Tizhong Shan

• Curated by eLife

  eLife Assessment

  This study provides valuable information on the single nucleus RNA sequencing transcriptome, pathways, and cell types in pig skeletal muscle in response to conjugated linoleic acid (CLA) supplementation. Based on the comprehensive data analyses, the data are considered compelling and provide new insight into the mechanisms underlying intramuscular fat deposition and muscle fiber remodeling. The study contributes significantly to the understanding of nutritional strategies for fat infiltration in pig muscle.

Reviewed by eLife

Med12 cooperates with multiple differentiation signals to facilitate efficient lineage transitions in embryonic stem cells

1. Max Fernkorn
2. Christian Schröter

Reviewed by Review Commons

Piezo1 balances membrane tension and cortical contractility to stabilize intercellular junctions and maintain epithelial barrier integrity

1. Ahsan Javed
2. Aki Stubb
3. Clémentine Villeneuve
4. Franziska Peters
5. Matthias Rübsam
6. Carien M. Niessen
7. Leah C. Biggs
8. Sara A. Wickström

Reviewed by Review Commons

Clathrin-independent endocytosis and retrograde transport in cancer cells promote cytotoxic CD8 T cell activation

1. Shiqiang Xu
2. Alix Buridant
3. Thibault Hirsch
4. Massiullah Shafaq-Zadah
5. Estelle Dransart
6. Benjamin Ledoux
7. Ludger Johannes
8. Pierre van der Bruggen
9. Pierre Morsomme
10. Henri-François Renard

• Curated by eLife

  eLife Assessment

  This valuable study provides convincing evidence that specific proteins on the surface of cancer cells undergo a particular form of recycling and are redirected toward the cell-cell contact with T cells, a type of immune cell. However, the characterization of the consequences of T cell activation resulting from perturbing the recycling pathway is incomplete. Furthermore, relevant literature has not been sufficiently cited.

Reviewed by eLife

The RAB27A effector SYTL5 regulates mitophagy and mitochondrial metabolism

1. Ana Lapao
2. Lauren Sophie Johnson
3. Laura Trachsel-Moncho
4. Sakshi Singh
5. Matthew Yoke Wui Ng
6. Sigve Nakken
7. Eeva-Liisa Eskelinen
8. Anne Simonsen

• Curated by eLife

  eLife Assessment

  This study presents valuable insights into the role of two proteins, Rab27A and SYTL5, which control vesicle transport and delivery. While the data is clear, the overall evidence is somewhat incomplete. Strengthening the mechanistic aspect would enhance the study, making it of greater interest to cell biologists studying membrane trafficking and mitochondria.

Reviewed by eLife

Control of ciliary transcriptional programs during spermatogenesis by antagonistic transcription factors

1. Weihua Wang
2. Junqiao Xing
3. Xiqi Zhang
4. Hongni Liu
5. Xingyu Liu
6. Haochen Jiang
7. Cheng Xu
8. Xue Zhao
9. Zhangfeng Hu

• Curated by eLife

  eLife Assessment

  This valuable study presents data suggesting the critical roles of two ancient proteins, XAP5 and XAP5L, in regulating the transcriptional program of ciliogenesis during mouse spermatogenesis. The supporting data are solid, and this work will be of interest to biomedical researchers studying ciliogenesis and reproduction.

Reviewed by eLife

Multi-omics analyses and machine learning prediction of oviductal responses in the presence of gametes and embryos

1. Ryan M Finnerty
2. Daniel J Carulli
3. Akshata Hedge
4. Yanli Wang
5. Frimpong Boadu
6. Sarayut Winuthayanon
7. Jianlin Jack Cheng
8. Wipawee Winuthayanon

• Curated by eLife

  eLife Assessment

  This important study reports the transcriptomic and proteomic landscapes of the oviducts at four different preimplantation stages during natural fertilization, pseudopregnancy, and superovulation. The supporting data are convincing. This work will be of interest to reproductive biologists and clinicians practicing reproductive medicine.

Reviewed by eLife

Enalapril mitigates senescence and aging-related phenotypes by targeting antioxidative genes via phosphorylated Smad1/5/9

1. Wencong Lyu
2. Haochen Wang
3. Zhehao Du
4. Ran Wei
5. Jianuo He
6. Fanju Meng
7. Jinlong Bi
8. Lijun Zhang
9. Chao Zhang
10. Yiting Guan
11. Wei Tao

• Curated by eLife

  eLife Assessment

  The manuscript presents valuable data suggesting that enalapril elevates pSmad1/5/9 signaling, reduces cellular senescence, and enhances physiological functions in aged mice. However, the in vivo evidence remains incomplete, as studies blocking pSmad1/5/9 or using NAC to negate enalapril's purported benefits are lacking, and no lifespan extension data are shown. While in vitro findings support Smad1/5/9 as a key mediator, additional experiments - including BMP receptor inhibition and comprehensive senescence markers - are necessary to validate its essential role in vivo. Overall, the study provides promising insights into enalapril's anti-senescence potential but requires further rigorous investigation to fully substantiate its mechanism and therapeutic impact.

Reviewed by eLife

Quantification of Cyclin-CDK dissociation constants in living cells using fluorescence cross-correlation spectroscopy with green and near-infrared fluorescent proteins

1. Aika Toyama
2. Yuhei Goto
3. Yuhei Yamauchi
4. Hironori Sugiyama
5. Yohei Kondo
6. Atsushi Mochizuki
7. Kazuhiro Aoki

Reviewed by Review Commons

Bridging the Gap in Cancer Cell Behavior Against Matrix Stiffening: Insights from a Trizonal Model

1. Mohammad E Torki
2. Fan Liu
3. Rongguang Xu
4. Yunfeng Chen
5. Jeffery Fredberg
6. Zi Chen

• Curated by eLife

  eLife Assessment

  This study presents a valuable nonlinear mathematical model that addresses how cell shape transitions in response to ECM stiffness. The evidence supporting the claims of the authors is solid, although additional work is required to improve the manuscript. For instance, the authors should improve the overall readability of the text and amend the experimental validation section. The work will be of interest to scientists working on a spectrum of fields including cell mechanics, cell behaviors, and cancer research.

Reviewed by eLife