Cell Biology

T-cell receptor (TCR) signaling promotes the assembly of RanBP2/RanGAP1-SUMO1/Ubc9 nuclear pore subcomplex via PKC-θ-mediated phosphorylation of RanGAP1

1. Yujiao He
2. Zhiguo Yang
3. Chen-si Zhao
4. Zhihui Xiao
5. Yu Gong
6. Yun-Yi Li
7. Yiqi Chen
8. Yunting Du
9. Dianying Feng
10. Amnon Altman
11. Yingqiu Li

• Curated by eLife

  Evaluation Summary:

  PKC-theta is known to regulate T cell activation, and this manuscript reveals a novel function of PKC-theta in the regulation of the nuclear pore complexes. The work by He and colleagues reveals that PKC-theta is recruited to the nuclear pore complex wherein it serves to regulate the assembly of key components of the RanBP2 subcomplex of the NPC, which in turn enables the translocation of AP1, NFkB and NFAT into the nucleus. However, these results need to be substantiated by additional experiments or by limiting the breath of the conclusions.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

CEM500K, a large-scale heterogeneous unlabeled cellular electron microscopy image dataset for deep learning

1. Ryan Conrad
2. Kedar Narayan

• Curated by eLife

  Evaluation Summary:

  This manuscript describes the curation of a training dataset that will be an important resource for developers of new segmentation and deep-learning algorithms for electron microscopy data. The small size of the dataset makes it easy to use, and its broad range of image modalities ensure that the model will be applicable in many situations, making it very useful for the community.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Adult stem cell-derived complete lung organoid models emulate lung disease in COVID-19

1. Courtney Tindle
2. MacKenzie Fuller
3. Ayden Fonseca
4. Sahar Taheri
5. Stella-Rita Ibeawuchi
6. Nathan Beutler
7. Gajanan Dattatray Katkar
8. Amanraj Claire
9. Vanessa Castillo
10. Moises Hernandez
11. Hana Russo
12. Jason Duran
13. Laura E Crotty Alexander
14. Ann Tipps
15. Grace Lin
16. Patricia A Thistlethwaite
17. Ranajoy Chattopadhyay
18. Thomas F Rogers
19. Debashis Sahoo
20. Pradipta Ghosh
21. Soumita Das

• Curated by eLife

  Evaluation Summary:

  The manuscript by Tindle et al describes generation of adult lung organoids (ALO) from human lung biopsies and their use to study the changes in gene expression as a result of SARS-CoV-2 infection. In this particular case the authors report the presence of AT1, AT2 cells, Basal cells, Goblet cells, Ciliated cells and Club cells. The authors were able to cultivate the cells at the air-liquid interface and to establish cultures of predominately proximal and predominately distal airway cells. The main finding is that proximal cells are more prone to viral infection, while distal cells are governing the exuberant inflammatory response, with both cells required for the exuberant response to occur. Useful information provided by the paper is the analysis gene signatures of various cellular models, in comparison to the infected human lung.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife, ScreenIT

CTP and parS coordinate ParB partition complex dynamics and ParA-ATPase activation for ParABS-mediated DNA partitioning

1. James A Taylor
2. Yeonee Seol
3. Jagat Budhathoki
4. Keir C Neuman
5. Kiyoshi Mizuuchi

• Curated by eLife

  Evaluation Summary:

  This manuscript reports carefully executed experiments on the dynamics of ParA-ParB and ParB-ParB interactions. Two main findings are presented: a change in stoichiometry of ParA-ParB interactions upon ligand binding and ligand dependent DNA condensation by ParB. The work is solid, the conclusions are generally well supported by the data, however, the relevance of some of the findings could be established more directly.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Single-cell transcriptomics defines heterogeneity of epicardial cells and fibroblasts within the infarcted murine heart

1. Julia Hesse
2. Christoph Owenier
3. Tobias Lautwein
4. Ria Zalfen
5. Jonas F Weber
6. Zhaoping Ding
7. Christina Alter
8. Alexander Lang
9. Maria Grandoch
10. Norbert Gerdes
11. Jens W Fischer
12. Gunnar W Klau
13. Christoph Dieterich
14. Karl Köhrer
15. Jürgen Schrader

• Curated by eLife

  Evaluation Summary:

  The article by Hesse et al. defines heterogeneity of epicardial cells and fibroblasts in a murine model of cardiac injury to analyze the resulting populations through single cell RNA sequencing. Spatial confirmation of associated markers is performed using in-situ RNA hybridization. The work provides new insights into the heterogeneity of epicardial stromal and activated cardiac stromal cells post-injury.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Misoprostol treatment prevents hypoxia-induced cardiac dysfunction through a 14-3-3 and PKA regulatory motif on Bnip3

1. Matthew D. Martens
2. Nivedita Seshadri
3. Lucas Nguyen
4. Donald Chapman
5. Elizabeth S. Henson
6. Bo Xiang
7. Landon Falk
8. Arielys Mendoza
9. Sunil Rattan
10. Jared T. Field
11. Philip Kawalec
12. Spencer B. Gibson
13. Richard Keijzer
14. Ayesha Saleem
15. Grant M. Hatch
16. Christine A. Doucette
17. Jason M. Karch
18. Vernon W. Dolinsky
19. Ian M. Dixon
20. Adrian R. West
21. Christof Rampitsch
22. Joseph W. Gordon

Reviewed by Review Commons

HDLs extract lipophilic drugs from cells

1. Adi Zheng
2. Gilles Dubuis
3. Maria Georgieva
4. Carla Susana Mendes Ferreira
5. Marc Serulla
6. Maria del Carmen Conde Rubio
7. Evgeniya Trofimenko
8. Thomas Mercier
9. Laurent Decosterd
10. Christian Widmann

Reviewed by Review Commons

Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish

1. Meredith H Wilson
2. Stephen C Ekker
3. Steven A Farber

• Curated by eLife

  Evaluation Summary:

  This manuscript has generated novel and useful tools to mark cytoplasmic lipid droplets and monitor their dynamics in various tissues in live animals. It will be of interest to researchers studying lipid metabolism and related human diseases.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Augmin deficiency in neural stem cells causes p53-dependent apoptosis and aborts brain development

1. Ricardo Viais
2. Marcos Fariña-Mosquera
3. Marina Villamor-Payà
4. Sadanori Watanabe
5. Lluís Palenzuela
6. Cristina Lacasa
7. Jens Lüders

Reviewed by Review Commons

Latrophilin GPCR signaling mediates synapse formation

1. Richard Sando
2. Thomas C Südhof

• Curated by eLife

  Evaluation Summary:

  The main finding that GPCR activity is necessary for latrophilins' role in synapse formation is both surprising and important. This work will inspire new research on compartmentalized GPCR signaling at the synapse.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife