Cell Biology

The ATF6β-calreticulin axis promotes neuronal survival under endoplasmic reticulum stress and excitotoxicity

1. Dinh Thi Nguyen
2. Thuong Manh Le
3. Tsuyoshi Hattori
4. Mika Takarada-Iemata
5. Hiroshi Ishii
6. Jureepon Roboon
7. Takashi Tamatani
8. Takayuki Kannon
9. Kazuyoshi Hosomichi
10. Atsushi Tajima
11. Shusuke Taniuchi
12. Masato Miyake
13. Seiichi Oyadomari
14. Takashi Tanaka
15. Nobuo Kato
16. Shunsuke Saito
17. Kazutoshi Mori
18. Osamu Hori

Reviewed by Review Commons

Neuronal regulated ire-1-dependent mRNA decay controls germline differentiation in Caenorhabditis elegans

1. Mor Levi-Ferber
2. Rewayd Shalash
3. Adrien Le-Thomas
4. Yehuda Salzberg
5. Maor Shurgi
6. Jennifer IC Benichou
7. Avi Ashkenazi
8. Sivan Henis-Korenblit

• Curated by eLife

  Evaluation Summary:

  The authors of this manuscript previously showed that ER stress, and in particular the ER stress sensor Ire1, regulates transdifferentiation in C. elegans, leading to the ectopic differentiation of germline cells. In this follow-up manuscript, the authors present several lines of evidence supporting the idea that Ire1 modules these effects through degrading a novel mRNA substrate flp6. The authors identify the neurons and neuromodulators that affect accumulation of abnormal germline cells. The reviewers agreed that the discovery that flp6 is a regulated Ire-1-dependent decay target in C. elegans, and the demonstration of a non-cell-autonomous effect of Ire1 activity, are novel and likely to be of interest to a broad readership. However, more evidence is required to support some of the main conclusions.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

APEX-Gold: A genetically-encoded particulate marker for robust 3D electron microscopy

1. James Rae
2. Charles Ferguson
3. Nicholas Ariotti
4. Richard I. Webb
5. Han-Hao Cheng
6. James L. Mead
7. Jamie Riches
8. Dominic J.B. Hunter
9. Nick Martel
10. Joanne Baltos
11. Arthur Christopoulos
12. Nicole S. Bryce
13. Maria Lastra Cagigas
14. Sachini Fonseka
15. Edna C. Hardeman
16. Peter W. Gunning
17. Yann Gambin
18. Thomas Hall
19. Robert G. Parton

• Curated by eLife

  Summary: The manuscript by Rae et al. reports a new protocol for labeling genetically-tagged proteins of interest with heavy atom particles for visualization by electron microscopy. The optimized protocol builds on the use of the enzyme APEX2, fused to the target protein of interest. The contrast enhancement may be useful in diverse 3D EM techniques. Also, reviewers were enthusiastic about the prospects for quantitative studies, even for low-levels of endogenous expression. Semi-quantitative studies may be enabled because the new method appears to improve the proportionality of the signal such that the number of APEX2 tags in a sample correlates with the number of heavy atom particles. The apparent simplicity of the protocol raises the potential for it to become a standard in the field of EM labeling.

  Reviewer #1, Reviewer #2 and Reviewer #3 opted to reveal their name to the authors in the decision letter after review.

Reviewed by eLife

KLF10 integrates circadian timing and sugar signaling to coordinate hepatic metabolism

1. Anthony A Ruberto
2. Aline Gréchez-Cassiau
3. Sophie Guérin
4. Luc Martin
5. Johana S Revel
6. Mohamed Mehiri
7. Malayannan Subramaniam
8. Franck Delaunay
9. Michèle Teboul

• Curated by eLife

  Evaluation Summary:

  This paper will be of interest in the fields of circadian biology and metabolic physiology. It provides a molecular mechanism for protection against development of fatty liver in response to high sugar consumption. Quality data support the key claims of the paper in each of the main research areas (circadian biology and metabolism) but additional efforts are needed to integrate the two parts. The current study does not thoroughly connect the in vitro and in vivo findings and misses the opportunity to fully characterize the role of KLF10 in circadian regulation in response to excessive sugar consumption.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Augmenter of liver regeneration regulates cellular iron homeostasis by modulating mitochondrial transport of ATP-binding cassette B8

1. Hsiang-Chun Chang
2. Jason Solomon Shapiro
3. Xinghang Jiang
4. Grant Senyei
5. Teruki Sato
6. Justin Geier
7. Konrad T Sawicki
8. Hossein Ardehali

• Curated by eLife

  Evaluation Summary:

  This is an interesting manuscript and experiments generally make their point on Alr effects. However, additional data would strengthen the paper with respect to the relative roles of cytoplasmic vs mitochondrial isoforms as would mitochondrial function studies.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Endothelial cell senescence exacerbates pulmonary hypertension through Notch-mediated juxtacrine signaling

1. Risa Ramadhiani
2. Koji Ikeda
3. Kazuya Miyagawa
4. Gusty Rizky Teguh Ryanto
5. Naoki Tamada
6. Yoko Suzuki
7. Ken-ichi Hirata
8. Noriaki Emoto

Reviewed by eLife

Decreasing pdzd8-mediated mito–ER contacts improves organismal fitness and mitigates Aβ ₄₂ toxicity

1. Victoria L Hewitt
2. Leonor Miller-Fleming
3. Madeleine J Twyning
4. Simonetta Andreazza
5. Francesca Mattedi
6. Julien Prudent
7. Franck Polleux
8. Alessio Vagnoni
9. Alexander J Whitworth

Reviewed by Review Commons

Genetic and pharmacological evidence for kinetic competition between alternative poly(A) sites in yeast

1. Rachael Emily Turner
2. Paul F Harrison
3. Angavai Swaminathan
4. Calvin A Kraupner-Taylor
5. Belinda J Goldie
6. Michael See
7. Amanda L Peterson
8. Ralf B Schittenhelm
9. David R Powell
10. Darren J Creek
11. Bernhard Dichtl
12. Traude H Beilharz

• Curated by eLife

  Evaluation Summary:

  This study aims to provide a comprehensive analysis of factors governing polyadenylation site selection in yeast. Overall, the authors reveal that multiple but distinct inputs including polyadenylation machinery integrity, transcription elongation rate, nucleotide availability and chromatin landscape all contribute to controlling cleavage and polyadenylation.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

The recycling endosome protein Rab25 coordinates collective cell movements in the zebrafish surface epithelium

1. Patrick Morley Willoughby
2. Molly Allen
3. Jessica Yu
4. Roman Korytnikov
5. Tianhui Chen
6. Yupeng Liu
7. Isis So
8. Haoyu Wan
9. Neil Macpherson
10. Jennifer A Mitchell
11. Rodrigo Fernandez-Gonzalez
12. Ashley EE Bruce

• Curated by eLife

  Evaluation Summary:

  Willoughby et al. examine the role of Rab25 in early embryogenesis in zebrafish. They implicate Rab25 activity in abscission and show various defects including delayed epiboly and altered cell behaviors associated with defective acting dynamics. Overall, this is an interesting and well-written paper. However, there are a number of important controls that are missing and some connections such as the implication of membrane recycling that require stronger experimental validation.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Micronuclei in Kif18a mutant mice form stable micronuclear envelopes and do not promote tumorigenesis

1. Leslie A. Sepaniac
2. Whitney Martin
3. Louise A. Dionne
4. Timothy M. Stearns
5. Laura G. Reinholdt
6. Jason Stumpff

Reviewed by Review Commons