Cell Biology

Treacle and MDC1 coordinate rDNA break repair by homologous recombination

1. Andrea Haenel
2. Johannes Leyrer
3. Manuel Stucki

• Curated by eLife

  eLife Assessment

  This manuscript reports valuable results on the role of MDC1 and Treacle in DSB repair in rDNA repeats. It has been previously established that MDC1 is replaced by Treacle as the main adaptor in the nucleolar DNA damage response. This work provides convincing evidence that MDC1 is required for the recruitment of RAD51 and BRCA1 to DSBs in rDNA. The work involves multiple MDC1 knockout models and establishes that RFN8-RNF168 act downstream of MDC1 in the recruitment of the HR machinery to nucleolar DSBs.

Reviewed by eLife

Aging-associated Increase of GATA4 levels in Articular Cartilage is Linked to Impaired Regenerative Capacity of Chondrocytes and Osteoarthritis

1. Meagan J Makarczyk
2. Yiqian Zhang
3. Alyssa Aguglia
4. Olivia Bartholomew
5. Sophie Hines
6. Kate Li
7. Suyash Sinkar
8. Silvia Liu
9. Craig Duvall
10. Hang Lin

• Curated by eLife

  eLife Assessment

  This study presents an important finding on the role of GATA4 in aging- and OA-associated cartilage pathology. The conclusions are well supported by compelling in vitro and in vivo evidence. This work will be of broad interest to both cell biologists and orthopedic/skeletal health clinicians.

Reviewed by eLife

Canonical and phosphoribosyl ubiquitination coordinate to stabilize a proteinaceous structure surrounding the Legionella-containing vacuole

1. Adriana Steinbach
2. Chetan Mokkapati
3. Shaeri Mukherjee

• Curated by eLife

  eLife Assessment

  This manuscript presents a valuable and insightful contribution to the understanding of how Legionella pneumophila remodels its vacuolar niche through coordinated ubiquitination mechanisms. The identification of Rab5 as a target of both canonical and phosphoribosyl ubiquitination, and the demonstration of a detergent-resistant ubiquitin "cloud" surrounding the LCV, represent significant advances in the field. The findings are supported by rigorous experimental design, robust quantitative analyses, and clear mechanistic insight, meeting a standard of evidence that is compelling and exceeds current state-of-the-art approaches.

Reviewed by eLife

Human receptive endometrial assembloid for deciphering the implantation window

1. Yu Zhang
2. Rusong Zhao
3. Chaoyan Yang
4. Jinzhu Song
5. Peishu Liu
6. Yan Li
7. Boyang Liu
8. Tao Li
9. Changjian Yin
10. Minghui Lu
11. Zhenzhen Hou
12. Chuanxin Zhang
13. Zi-Jiang Chen
14. Keliang Wu
15. Han Zhao

• Curated by eLife

  eLife Assessment

  This important study reports an endometrial organoid culture system mimicking the window of implantation. The evidence supporting the conclusion drawn is convincing. The data will be of interest to embryologists and investigators working on reproductive biology and medicine.

Reviewed by eLife

Paclitaxel compromises nuclear integrity in interphase through SUN2-mediated cytoskeletal coupling

1. Thomas Hale
2. Victoria L Hale
3. Piotr Kolata
4. Ália dos Santos
5. Matteo Allegretti

Reviewed by preLights, Review Commons

Dissociation of the nuclear basket triggers chromosome loss in aging yeast

1. Mihailo Mirkovic
2. Jordan McCarthy
3. Anne Cornelis Meinema
4. Julie Parenteau
5. Sung Sik Lee
6. Sherif Abou Elela
7. Yves Barral

• Curated by eLife

  eLife Assessment

  This fundamental study reveals that aging in yeast leads to chromosome mis-segregation due to asymmetric partitioning of chromosomes, driven by disruption of the nuclear pore complex and pre-mRNA leakage. The findings are convincingly supported by carefully-designed experimental data with a combination of genetic, molecular biology and cell biology approaches.

Reviewed by eLife

Nucleus softens during herpesvirus infection

1. Aapo Tervonen
2. Visa Ruokolainen
3. Simon Leclerc
4. Katie Tieu
5. Sébastien Lyonnais
6. Henri Niskanen
7. Jian-Hua Chen
8. Alka Gupta
9. Minna U Kaikkonen
10. Carolyn A Larabell
11. Delphine Muriaux
12. Salla Mattola
13. Daniel E Conway
14. Teemu O Ihalainen
15. Vesa Aho
16. Maija Vihinen-Ranta

Reviewed by PREreview

Targeting ZNRF3 and RNF43 to Restore Regeneration and Reverse Metabolic Dysfunction-Associated Steatotic Liver Disease

1. Federico Di Tullio
2. Sue Bin Yang
3. Lida Yang
4. Bruno Cogliati
5. Shamsa Roshan
6. Kaiyuan Guo
7. Abigail Glezer
8. Jonathan Conrad
9. Siddarth Vinod Kumar
10. Joana Almeida
11. Haoyuan Li
12. Shahina Saeed
13. Marina Barcena-varela
14. Emily Bramel
15. Anthony Lozano
16. Lianyong Su
17. Derrick Zhao
18. Huong Pham
19. Fanglin Ma
20. Amaia Lujambio
21. Sai Ma
22. Yizhou Dong
23. Huiping Zhou
24. Tianliang Sun

Reviewed by PREreview

Orderly mitosis shapes interphase genome architecture

1. Krishnendu Guin
2. Adib Keikhosravi
3. Raj Chari
4. Gianluca Pegoraro
5. Tom Misteli

• Curated by eLife

  eLife Assessment

  This valuable study combines microscopy and CRISPR screening in two different cell lines to identify factors involved in global chromatin organization, using centromere clustering as a proxy. Follow-up cell cycle synchronisation studies confirm roles in centromere clustering in mitosis. However, incomplete characterisation of the cell lines used limits the interpretation of the findings. The study will interest researchers studying genome organisation in mitosis.

Reviewed by eLife

Spatial control of secretory vesicle targeting by the Ync13–Rga7– Rng10 complex during cytokinesis

1. Sha Zhang
2. Davinder Singh
3. Yi-Hua Zhu
4. Katherine J. Zhang
5. Alejandro Melero
6. Sophie G. Martin
7. Jian-Qiu Wu

Reviewed by Review Commons