Cell Biology

Compartmentalized Cytoplasmic Flows Direct Protein Transport to the Cell’s Leading Edge

1. Catherine G. Galbraith
2. Brian P. English
3. Ulrike Boehm
4. James A. Galbraith

Reviewed by Arcadia Science

Rudhira-mediated microtubule stability controls TGFβ signaling during mouse vascular development

1. Divyesh Joshi
2. Preeti Jindal
3. Ronak Shetty
4. Maneesha S. Inamdar

• Curated by eLife

  eLife assessment

  This important work provides another layer of regulatory mechanism for TGF-beta signaling activity. The evidence supports the involvement of microtubules as a reservoir of Smad2/3, however, additional evidence to convincingly demonstrate the functional involvement of Rudhira in this process is highly appreciated. The work will be of broad interest to developmental biologists in general and molecular biologists in the field of growth factor signaling.

Reviewed by eLife

Novel Mechanism for Tubular Injury in Nephropathic Cystinosis

1. Swastika Sur
2. Maggie Kerwin
3. Silvia Pineda
4. Poonam Sansanwal
5. Tara K. Sigdel
6. Marina Sirota
7. Minnie M. Sarwal

• Curated by eLife

  eLife assessment

  This important study addresses the idea that defective lysosomal clearance might be causal to renal dysfunction in cystinosis. They observe that restoring expression of vATPase subunits and treatment with Astaxanthin ameliorate mitochondrial function in a model of renal epithelial cells, opening opportunities for translational application to humans. The data are convincing, but the description of methodologies is incomplete.

Reviewed by eLife

A delta-tubulin/epsilon-tubulin/Ted protein complex is required for centriole architecture

1. Rachel Pudlowski
2. Lingyi Xu
3. Ljiljana Milenkovic
4. Katherine Hemsworth
5. Tim Stearns
6. Jennifer T. Wang

• Curated by eLife

  eLife assessment

  The study by Pudlowski et al. shows that a protein complex composed of delta- and epsilon-tubulin together with TEDC1 and TEDC2, which was previously identified, functions in generating centriolar triplet microtubules, and that this is crucial for the proper formation of centriolar subdomains and the stability of centrioles throughout the cell cycle. The findings are valuable for a better understanding of centriole biogenesis and structure and are largely supported by solid evidence based on knockout cell lines, immunoprecipitation, and ultrastructure expansion microscopy. The work is of interest to cell biologists, in particular researchers with interest in centrosome biology.

Reviewed by eLife

Phosphorylation of Aly3 C-terminus impedes aberrant endocytosis of S. pombe hexose transporter Ght5

1. Yusuke Toyoda
2. Fumie Masuda
3. Shigeaki Saitoh

Reviewed by Review Commons

TPR is required for cytoplasmic chromatin fragment formation during senescence

1. Bethany M. Bartlett
2. Yatendra Kumar
3. Shelagh Boyle
4. Tamoghna Chowdhury
5. Andrea Quintanilla
6. Charlene Boumendil
7. Juan Carlos Acosta
8. Wendy A. Bickmore

Reviewed by Review Commons

Editing of endogenous tubulins reveals varying effects of tubulin posttranslational modifications on axonal growth and regeneration

1. Yu-Ming Lu
2. Shan Yan
3. Shih-Chieh Ti
4. Chaogu Zheng

• Curated by eLife

  eLife assessment

  This fundamental study analyzes the roles of post-translational modifications of tubulin by generating a large panel of tubulin mutants and describing their effects on morphogenesis and function of sensory neurons in C. elegans. The work, which is of interest to all cell biologists, in particular researchers with an interest in the microtubule cytoskeleton and neurobiology, presents conclusions that are supported by solid evidence. Demonstrating that all introduced mutations have the intended consequences and exploring their direct effect on microtubules would further increase the impact of the work.

Reviewed by eLife

Heparan sulfate-dependent phase separation of CCL5 and its chemotactic activity

1. Xiaolin Yu
2. Guangfei Duan
3. Pengfei Pei
4. Long Chen
5. Renji Gu
6. Wenrui Hu
7. Hongli Zhang
8. Yan-Dong Wang
9. Lili Gong
10. Lihong Liu
11. Ting-Ting Chu
12. Jin-Ping Li
13. Shi-Zhong Luo

• Curated by eLife

  eLife assessment

  How the triplicate interaction between chemokines with both GAGs and G protein-coupled receptors (GPCR) works and how gradients are created and potentially maintained in vivo are poorly understood. The authors provide solid evidence to show phase separation can drive chemotactic gradient formation. The paper is a useful advance in the field of chemokine biology.

Reviewed by eLife

Assessment of the Epigenomic Landscape in Human Myometrium at Term Pregnancy

1. San-Pin Wu
2. Elvis Quiroz
3. Tianyuan Wang
4. Skylar Montague Redecke
5. Xin Xu
6. Lin Lin
7. Matthew L. Anderson
8. Francesco J. DeMayo

• Curated by eLife

  eLife assessment

  This valuable study adopted a multi-omics approach to elucidate the regulatory mechanism underlying parturition and myometrial quiescence. The data presented to support the main conclusion remains incomplete. This work will be of interest to both basic researchers who work on reproductive biology and clinicians who practice reproductive medicine.

Reviewed by eLife

The endoplasmic reticulum stress sensor IRE1 regulates collagen secretion through the enforcement of the proteostasis factor P4HB/PDIA1 contributing to liver damage and fibrosis

1. Younis Hazari
2. Hery Urra
3. Valeria A. Garcia Lopez
4. Javier Diaz
5. Giovanni Tamburini
6. Mateus Milani
7. Philippe Pihan
8. Sylvere Durand
9. Fanny Aprahamia
10. Reese Baxter
11. Menghao Huang
12. X Charlie Dong
13. Helena Vihinen
14. Ana Batista-Gonzalez
15. Patricio Godoy
16. Alfredo Criollo
17. Vlad Ratziu
18. Fabienne Foufelle
19. Jan G. Hengstler
20. Eija Jokitalo
21. Beatrice Bailly-maitre
22. Jessica L Maiers
23. Lars Plate
24. Guido Kroemer
25. Claudio Hetz

Reviewed by Review Commons