Cell Biology

Cell crowding activates pro-invasive mechanotransduction pathway in high-grade DCIS via TRPV4 inhibition and cell volume reduction

1. Xiangning Bu
2. Nathanael Ashby
3. Teresa Vitali
4. Sulgi Lee
5. Ananya Gottumukkala
6. Kangsun Yun
7. Sana Tabbara
8. Patricia Latham
9. Christine Teal
10. Inhee Chung

• Curated by eLife

  eLife Assessment

  This fundamental study provides compelling evidence that TRPV4 plays a crucial role in mechanical sensing during cancer cell transition from non-invasive to invasive states, and offers novel insights into metastasis. By employing multiple experimental approaches, including pharmacological and genetic manipulation, as well as advanced imaging techniques, the authors demonstrate a strong correlation between TRPV4 dynamics, calcium homeostasis, and cell volume plasticity. The findings significantly enhance our understanding of mechanotransduction in cancer and present TRPV4 as a promising therapeutic target for inhibiting metastasis.

Reviewed by eLife

Subcellular Region Morphology Reflects Cellular Identity

1. Ángel-Carlos Román
2. Alba Diaz-Pizarro
3. Nuria Del Valle-Del Pino
4. Marcos Olivera-Gómez
5. Guadalupe Cumplido-Laso
6. Dixan Agustín Benítez
7. Jose María Carvajal-González
8. Sonia Mulero-Navarro

Reviewed by Review Commons

Stratification of enterochromaffin cells by single-cell expression analysis

1. Yan Song
2. Linda J Fothergill
3. Kari S Lee
4. Brandon Y Liu
5. Ada Koo
6. Mark Perelis
7. Shanti Diwakarla
8. Brid Callaghan
9. Jie Huang
10. Jill Wykosky
11. John B Furness
12. Gene W Yeo

• Curated by eLife

  eLife Assessment

  This important study presents a transcriptomic analysis of enterochromaffin cells in the intestine. The evidence supporting the authors' claims is solid, although the functional analysis is focused on the Piezo2-expressing subset in the colon. The work will be of interest to biologists working on intestinal mucosal biology.

Reviewed by eLife

Capsaicin acts as a novel NRF2 agonist to suppress ethanol induced gastric mucosa oxidative damage by directly disrupting the KEAP1-NRF2 interaction

1. Xiaoning Gao
2. WuYan Guo
3. Peiyuan Liu
4. Mingyue Yuwen
5. Zixiang Liu
6. Ruyang Tan
7. Kairui Liu
8. Zhiru Yang
9. Junli Ba
10. Xue Bai
11. Shiti Shama
12. Cong Tang
13. Kai Miao
14. Haozhi Pei
15. Liren Liu
16. Cheng Zhu
17. Tao Wang
18. Bo Zhang
19. Jun Kang

• Curated by eLife

  eLife Assessment

  This valuable study suggests that capsaicin nanoparticle administration in rats activates the transcription factor Nrf2 by directly binding to its repressor, KEAP1, leading to the induction of cytoprotective genes and preventing alcohol-induced gastric damage, offering a potential avenue for treating alcoholism-related gastric disorders. Although improvements were made following the first revision, the evidence supporting capsaicin as an Nrf2 activator remains incomplete, as some methodological aspects still require revision and the interpretation of key data needs further clarification.

Reviewed by eLife

Cytosolic Carboxypeptidase 5 maintains mammalian ependymal multicilia to ensure proper homeostasis and functions of the brain

1. Rubina Dad
2. Yujuan Wang
3. Chuyu Fang
4. Yuncan Chen
5. Yuan Zhang
6. Xinwen Pan
7. Xinyue Zhang
8. Emily Swanekamp
9. Krish Patel
10. Matthias TF Wolf
11. Zhiguang Yuchi
12. Xueliang Zhu
13. Hui-Yuan Wu

• Curated by eLife

  eLife Assessment

  This is a valuable study that explores the function of CCP5 in mouse ependymal cells. The methods, data, and analyses broadly support the claims. However, the study is incomplete as it stands. Minor weaknesses remain and the authors may wish to address them.

Reviewed by eLife

Proteome dynamics reveal Leiomodin 1 as a key regulator of myogenic differentiation

1. Ellen Späth
2. Svenja C Schüler
3. Ivonne Heinze
4. Therese Dau
5. Alberto Minetti
6. Maleen Hofmann
7. Julia von Maltzahn
8. Alessandro Ori

• Curated by eLife

  eLife Assessment

  This valuable study offers insights into the role of Leiomodin-1 (LMOD1) in muscle stem cell biology, advancing our understanding of myogenic differentiation and indicating LMOD1 as a regulator of muscle regeneration, aging, and exercise adaptation. The integration of in vitro and in vivo approaches, complemented by proteomic and imaging methodologies, is solid. However, certain aspects require further attention to improve the clarity, impact, and overall significance of the work, particularly in substantiating the in vivo relevance. This work will provide a starting point that will be of value to medical biologists and biochemists working on LMOD and its variants in muscle biology.

Reviewed by eLife

Spatially resolved rewiring of mitochondria-lipid droplet interactions in hepatic lipid homeostasis

1. Sun Woo Sophie Kang
2. Lauryn A Brown
3. Colin B Miller
4. Katherine M Barrows
5. Jihye L Golino
6. Constance M Cultraro
7. Daniel Feliciano
8. Mercedes B. Cornelius-Muwanuzi
9. Andy D Tran
10. Michael Kruhlak
11. Alexei Lobanov
12. Maggie Cam
13. Natalie Porat-Shliom

Reviewed by PREreview

Image-based identification and isolation of micronucleated cells to dissect cellular consequences

1. Lucian DiPeso
2. Sriram Pendyala
3. Heather Z Huang
4. Douglas M Fowler
5. Emily M Hatch

• Curated by eLife

  eLife Assessment

  This valuable paper reports machine learning-based image analysis pipelines for the automated segmentation of micronuclei and the detection and sorting of micronuclei-containing cells. These are powerful new tools for researchers who study micronuclei and their physiologic consequences. The analysis of the new tools and their benchmarking is rigorous and convincing; applications and remaining limitations are well explained in the paper.

Reviewed by eLife

Adventitial fibroblasts direct smooth muscle cell-state transition in pulmonary vascular disease

1. Slaven Crnkovic
2. Helene Thekkekara Puthenparampil
3. Shirin Mulch
4. Valentina Biasin
5. Nemanja Radic
6. Jochen Wilhelm
7. Marek Bartkuhn
8. Ehsan Bonyadi Rad
9. Alicja Wawrzen
10. Ingrid Matzer
11. Ankita Mitra
12. Ryan D Leib
13. Bence Miklos Nagy
14. Anita Sahu-Osen
15. Francesco Valzano
16. Natalie Bordag
17. Matthias Evermann
18. Konrad Hoetzenecker
19. Andrea Olschewski
20. Senka Ljubojevic-Holzer
21. Malgorzata Wygrecka
22. Kurt Stenmark
23. Leigh M Marsh
24. Vinicio de Jesus Perez
25. Grazyna Kwapiszewska

• Curated by eLife

  eLife Assessment

  This fundamental research conducted a molecular comparison between smooth muscle cells and adjacent fibroblast cells within lung blood vessels affected by pulmonary arterial hypertension. The study identified distinct disease-related states in each cell type and provided deeper insights into their interactions and communication. While certain conclusions should be interpreted with caution due to inherent methodological limitations, the study's findings remain convincing and robust. This is supported by the use of advanced and complementary techniques, as well as the rare isolation of diseased lung blood vessel cells from the same donor, enabling direct comparison.

Reviewed by eLife

Nuclear Argonaute protein NRDE-3 switches small RNA partners during embryogenesis to mediate temporal-specific gene regulatory activity

1. Shihui Chen
2. Carolyn Marie Phillips

• Curated by eLife

  eLife Assessment

  The study by Chen and Phillips provides evidence for a dynamic switch in the small RNA repertoire of the Argonaute protein NRDE-3 during embryogenesis in C. elegans. The work is supported by convincing experimental data, shedding light on RNA regulation during development. While the functional relevance of this process warrants further investigation, this study provides valuable insights into small RNA pathways with broader implications for developmental biology and gene regulation in other systems.

Reviewed by eLife