Cell Biology

A diabetic milieu increases cellular susceptibility to SARS-CoV-2 infections in engineered human kidney organoids and diabetic patients

1. Elena Garreta
2. Patricia Prado
3. Megan Stanifer
4. Vanessa Monteil
5. Carmen Hurtado del Pozo
6. Asier Ullate-Agote
7. Amaia Vilas-Zornoza
8. Juan Pablo Romero
9. Gustav Jonsson
10. Roger Oria
11. Alexandra Leopoldi
12. Astrid Hagelkruys
13. Daniel Moya-Rull
14. Federico González
15. Andrés Marco
16. Carolina Tarantino
17. Pere Domingo-Pedrol
18. Omar HasanAli
19. Pedro Ventura-Aguiar
20. Josep María Campistol
21. Felipe Prosper
22. Ali Mirazimi
23. Steeve Boulant
24. Josef M. Penninger
25. Nuria Montserrat

Reviewed by ScreenIT

SARS-CoV-2 Spike S1 glycoprotein is a TLR4 agonist, upregulates ACE2 expression and induces pro-inflammatory M ₁ macrophage polarisation

1. Mohamed M. Aboudounya
2. Mark R. Holt
3. Richard J. Heads

Reviewed by ScreenIT

Screening of cell-virus, cell-cell, gene-gene cross-talks among kingdoms of life at single cell resolution

1. Dongsheng Chen
2. Zhihua Ou
3. Jiacheng Zhu
4. Peiwen Ding
5. Haoyu Wang
6. Lihua Luo
7. Xiangning Ding
8. Tianming Lan
9. Weiying Wu
10. Yuting Yuan
11. Wendi Wu
12. Jiaying Qiu
13. Yixin Zhu
14. Yi Jia
15. Yanan Wei
16. Qiuyu Qin
17. Runchu Li
18. Chengcheng Sun
19. Wandong Zhao
20. Zhiyuan Lv
21. Mingyi Pu
22. Shangchen Yang
23. Ashley Chang
24. Xiaofeng Wei
25. Fengzhen Chen
26. Tao Yang
27. Zhenyong Wei
28. Fan Yang
29. Yuejiao Li
30. Yan Hua
31. Huan Liu

Reviewed by ScreenIT

Spatial transcriptomic and single-nucleus analysis reveals heterogeneity in a gigantic single-celled syncytium

1. Tobias Gerber
2. Cristina Loureiro
3. Nico Schramma
4. Siyu Chen
5. Akanksha Jain
6. Anne Weber
7. Anne Weigert
8. Malgorzata Santel
9. Karen Alim
10. Barbara Treutlein
11. J Gray Camp

• Curated by eLife

  Evaluation Summary:

  Single celled organisms are assumed to be smaller, simpler, and less complex than multicellular organisms like animals. However, there are many examples of large single-celled protists - especially amoeba - that can be up to centimeters in size, and it remains unclear how they are able to achieve these sizes and differentiated regions like tissues in animals. Here, the authors provide evidence for variation in gene expression in the syncytial (multinucleate) large amoeba Physarum polycephalum. While primarily descriptive work, the authors are claiming a provocative mechanistic interpretation of the single cell gene expression results, but not yet supported by the current data. This study is neverhteless elegant and interesting regarding heterogeneity of gene expression patterns and thus specialization of functions within a syncytial organism.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Condensation properties of stress granules and processing bodies are compromised in myotonic dystrophy type 1

1. Selma Gulyurtlu
2. Monika S. Magon
3. Patrick Guest
4. Panagiotis P. Papavasiliou
5. Kim D. Morrison
6. Alan R. Prescott
7. Judith E. Sleeman

Reviewed by Review Commons

Optical nanoscopy reveals SARS-CoV-2-induced remodeling of human airway cells

1. Wilco Nijenhuis
2. Hugo G.J. Damstra
3. Emma J. van Grinsven
4. Malina K. Iwanski
5. Patrique Praest
6. Zahra E. Soltani
7. Mariëlle M.P. van Grinsven
8. Jesse E. Brunsveld
9. Theun de Kort
10. Lisa W. Rodenburg
11. Dorien C.M. de Jong
12. Henriette H.M. Raeven
13. Sacha Spelier
14. Gimano D. Amatngalim
15. Anna Akhmanova
16. Monique Nijhuis
17. Robert Jan Lebbink
18. Jeffrey M. Beekman
19. Lukas C. Kapitein

Reviewed by ScreenIT

Tricalbin proteins regulate plasma membrane phospholipid homeostasis

1. Ffion B Thomas
2. Deike J Omnus
3. Jakob M Bader
4. Gary HC Chung
5. Nozomu Kono
6. Christopher J Stefan

Reviewed by Review Commons

Near-infrared imaging in fission yeast using a genetically encoded phycocyanobilin biosynthesis system

1. Keiichiro Sakai
2. Yohei Kondo
3. Hiroyoshi Fujioka
4. Mako Kamiya
5. Kazuhiro Aoki
6. Yuhei Goto

Reviewed by Review Commons

TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis

1. Zhong-Jiao Jiang
2. Wenping Li
3. Li-Hua Yao
4. Badeia Saed
5. Yan Rao
6. Brian S Grewe
7. Andrea McGinley
8. Kelly Varga
9. Simon Alford
10. Ying S Hu
11. Liang-Wei Gong

• Curated by eLife

  Evaluation Summary:

  Jiang et al. present a study on a novel role of the ion channel TRPM7 in the regulated release of neurotransmitters and hormones. Their analysis focused on TRPM7-deficient chromaffin cells and neurons, which they studied using electrophysiological and imaging techniques. The data indicate that TRPM7 is activated during the compensatory endocytotis process that follows secretory vesicle fusion. This TRPM7 activation increases Ca2+ influx into the cytosol during endocytosis, which boosts endocytosis and reduces the tendency of synapses to progressively fatigue in phases of prolonged stimulation. Multiple aspects of the present findings are novel, interesting, and important - they provide important new insights into the mechanisms by which transmitter and hormone release is fine-tuned. The mechanism of how secretory vesicles, and in particular synaptic vesicles, recycle is important to understand, as impairment in vesicle cycling in turn impairs synaptic transmission, and therefore brain function. However, there are several issues that remain to be addressed before the present data fully justify the conclusions put forward by the authors.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

The spike protein of SARS-CoV-2 induces endothelial inflammation through integrin α5β1 and NF-κB signaling

1. Juan Pablo Robles
2. Magdalena Zamora
3. Elva Adan-Castro
4. Lourdes Siqueiros-Marquez
5. Gonzalo Martinez de la Escalera
6. Carmen Clapp

Reviewed by ScreenIT