Cell Biology

C-type natriuretic peptide facilitates autonomic Ca2+ entry in growth plate chondrocytes for stimulating bone growth

1. Yuu Miyazaki
2. Atsuhiko Ichimura
3. Ryo Kitayama
4. Naoki Okamoto
5. Tomoki Yasue
6. Feng Liu
7. Takaaki Kawabe
8. Hiroki Nagatomo
9. Yohei Ueda
10. Ichiro Yamauchi
11. Takuro Hakata
12. Kazumasa Nakao
13. Sho Kakizawa
14. Miyuki Nishi
15. Yasuo Mori
16. Haruhiko Akiyama
17. Kazuwa Nakao
18. Hiroshi Takeshima

• Curated by eLife

  Evaluation Summary:

  The manuscript provides novel data to support the role of CNP (C Type Natriuretic peptide) in the proliferation and stimulation of growth plate chondrocytes for development of bone. The methods used are innovative and the data provided support the overall hypothesis of the study presented in the paper, which should be of broad interest to bone and cartilage biologists.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

A phosphoswitch at acinus-serine437 controls autophagic responses to cadmium exposure and neurodegenerative stress

1. Nilay Nandi
2. Zuhair Zaidi
3. Charles Tracy
4. Helmut Krämer

• Curated by eLife

  Evaluation Summary:

  Initial insights were provided by yeast genetic experiments into the mechanisms of starvation-induced autophagy. Since malfunctioning of this process is involved in numerous diseases, there is a need for further understanding the role and regulation of autophagy in different physiological settings. This work convincingly shows that a newly identified phosphatase controls basal levels of autophagy via regulation of phospho-acinus levels and reveals how cadmium intoxication triggers a neuroprotective autophagic response in the popular animal model Drosophila.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A

1. Blake A Creighton
2. Simone Afriyie
3. Deepa Ajit
4. Cristine R Casingal
5. Kayleigh M Voos
6. Joan Reger
7. April M Burch
8. Eric Dyne
9. Julia Bay
10. Jeffrey K Huang
11. ES Anton
12. Meng-Meng Fu
13. Damaris N Lorenzo

• Curated by eLife

  Evaluation Summary:

  This paper addresses the mechanisms regulating cytoskeletal changes mediating neuronal branching and axon growth. The authors assess the role of the scaffolding protein giant ankyrin B in cortical neuron differentiation and present strong data implicating ankyrin B functions with the cell adhesion molecule/Sema3A receptor complex and an actin severing protein. With a better explanation of the function of ankyrin B in the Sema3A signaling pathway, this manuscript will be of interest to cell biologists and developmental neurobiologists working to uncover the biological mechanisms of early circuit development, and how these mechanisms relate to autism spectrum disorders.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Initial ciliary assembly in Chlamydomonas requires Arp2/3 complex–dependent endocytosis

1. Brae M. Bigge
2. Nicholas E. Rosenthal
3. Prachee Avasthi

• Curated by eLife

  Evaluation Summary:

  This manuscript addresses the role of the Arp2/3 complex in endocytic retrieval of ciliary precursors from the plasma membrane for use in assembly of Chlamydomonas cilia, a topic of broad interest to cell biologists. The manuscript can serve as basis for future research addressing whether Arp2/3 affects cilium biogenesis solely via endocytic retrieval or through additional mechanisms, and whether these findings apply to species other than Chlamydomonas.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Protein-lipid interaction at low pH induces oligomerization of the MakA cytotoxin from Vibrio cholerae

1. Aftab Nadeem
2. Alexandra Berg
3. Hudson Pace
4. Athar Alam
5. Eric Toh
6. Jörgen Ådén
7. Nikola Zlatkov
8. Si Lhyam Myint
9. Karina Persson
10. Gerhard Gröbner
11. Anders Sjöstedt
12. Marta Bally
13. Jonas Barandun
14. Bernt Eric Uhlin
15. Sun Nyunt Wai

• Curated by eLife

  Evaluation Summary:

  Proteins that form pores in biological membranes are found in diverse contexts, including pathogenic toxins that help infect or lyse target cells and organelles. The study by Nadeem et al. reports on the properties of an α-pore-forming toxin, MakA, produced by the human pathogen, V. cholerae. The study is a remarkable example of a pH-induced structural mechanism of membrane remodeling. The insights reported here will be of interest to a wide range of scientists studying host-pathogen interactions, membrane remodeling, and macromolecular structure.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

A novel live-cell imaging assay reveals regulation of endosome maturation

1. Maria Podinovskaia
2. Cristina Prescianotto-Baschong
3. Dominik P Buser
4. Anne Spang

• Curated by eLife

  Evaluation Summary:

  Endosome maturation in animal cells has been challenging to characterize by microscopy because the fluorescence patterns are complex and dynamic. This study uses acute ionophore treatment to generate enlarged early endosomes, whose behavior and maturation can then be readily tracked. The results offer new insights into several phenomena, including the regulation of endosomal acidification during the maturation process.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Gramicidin S and Melittin - Potential anti-viral therapeutic peptides to treat SARS-CoV-2 infection

1. Mohammed Ghalib
2. Yash Parekh
3. Sarena Banu
4. Sushma Ram
5. Ramakrishnan Nagaraj
6. Bokara Kiran Kumar
7. Mohammed M Idris

Reviewed by ScreenIT

Major Complex Trait for Early De Novo Programming ‘CoV-MAC-TED’ Detected in Human Nasal Epithelial Cells Infected by Two SARS-CoV-2 Variants Is Promising to Help in Designing Therapeutic Strategies

1. José Hélio Costa
2. Shahid Aziz
3. Carlos Noceda
4. Birgit Arnholdt-Schmitt

Reviewed by ScreenIT

Transcriptome data from human nasal epithelial cells infected by H3N2 influenza virus indicate early unbalanced ROS/RNA levels, temporarily increased aerobic fermentation linked to enhanced α-tubulin and rapid energy-dependent IRF9-marked immunization

1. José Hélio Costa
2. Shahid Aziz
3. Carlos Noceda
4. Birgit Arnholdt-Schmitt

Reviewed by ScreenIT

Small disulfide loops in peptide hormones mediate self-aggregation and secretory granule sorting

1. Jennifer Reck
2. Nicole Beuret
3. Erhan Demirci
4. Cristina Prescianotto-Baschong
5. Martin Spiess

Reviewed by Review Commons