Cell Biology

mTOR activation induces endolysosomal remodeling and nonclassical secretion of IL-32 via exosomes in inflammatory reactive astrocytes

1. Kun Leng
2. Brendan Rooney
3. Frank McCarthy
4. Wenlong Xia
5. Indigo V.L. Rose
6. Sophie Bax
7. Marcus Chin
8. Saeed Fathi
9. Kari A. Herrington
10. Manuel Leonetti
11. Aimee Kao
12. Stephen P.J. Fancy
13. Joshua E. Elias
14. Martin Kampmann

Reviewed by ASAPbio crowd review

Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans

1. Ian F Price
2. Hannah L Hertz
3. Benjamin Pastore
4. Jillian Wagner
5. Wen Tang

• Curated by eLife

  Evaluation Summary:

  The authors use proximity labeling and genetic experiments to identify and functionally characterize new components of C. elegans P granules. The conclusions of the paper are well-supported by the data. This work will be of broad interest to developmental biologists, particularly those interested in the formation and function of germ cells.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

The supramolecular landscape of growing human axons

1. Patrick C. Hoffmann
2. Stefano L. Giandomenico
3. Iva Ganeva
4. Michael R. Wozny
5. Magdalena Sutcliffe
6. Wanda Kukulski
7. Madeline A. Lancaster

• Curated by eLife

  Evaluation Summary:

  The authors have elegantly combined two techniques, air-liquid interface cerebral organoid (ALI-CO) with correlative light and electron cryo-microscopy (cryo-CLEM), to study the ultrastructure of developing human axons. The technique presented is useful and the data is of high quality and well presented. With a somewhat stronger demonstration of the molecular resolution achieved and a description of how this technique can be expanded to study other organoids or cellular structures in non-neuronal cells and tissues, this paper will be of broad interest to neuroscientists and those developing cryo-electron tomography methods.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Visualizing molecules of functional human profilin

1. Morgan L Pimm
2. Xinbei Liu
3. Farzana Tuli
4. Jennifer Heritz
5. Ashley Lojko
6. Jessica L Henty-Ridilla

• Curated by eLife

  Evaluation Summary:

  This paper will be of interest to a broad audience of cell biologists and biochemists who study the cytoskeleton. It reports the development and rigorous characterization of a fully functional, fluorescently labeled version of profilin that can be used to visualize profilin's dynamic interactions in live cells. Owing to profilin's dual functions in regulating actin and microtubule assembly, this technological development will be a useful tool for a wide range of studies aimed at understanding the role of the cytoskeleton in driving fundamental cellular processes.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife, ASAPbio crowd review

Phagocytic ‘teeth’ and myosin-II ‘jaw’ power target constriction during phagocytosis

1. Daan Vorselen
2. Sarah R Barger
3. Yifan Wang
4. Wei Cai
5. Julie A Theriot
6. Nils C Gauthier
7. Mira Krendel

• Curated by eLife

  Evaluation Summary:

  This study is of great interest to cell biologists studying phagocytosis. The work describes a new method for studying phagocytosis; the engulfment of large cargos such as pathogens by the immune system. With this new method, they describe a mechanical force at the rim of the phagocytic cup that function like teeth. This work will advance the field in a new direction.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Fluidics system for resolving concentration-dependent effects of dissolved gases on tissue metabolism

1. Varun Kamat
2. Brian M Robbings
3. Seung-Ryoung Jung
4. John Kelly
5. James B Hurley
6. Kenneth P Bube
7. Ian R Sweet

• Curated by eLife

  Evaluation Summary:

  This paper presents a flow method for measuring the effects of dissolved gases on tissues while having control over tissue concentration. Working with gases can be challenging. The improvements reported here incorporate technology that allows for metabolic characterization of mammalian tissues while precisely controlling the concentration of abundant gases (e.g., oxygen), as well as trace gases (e.g., hydrogen sulfide). The authors utilize their technology to investigate the metabolic impacts of dissolved hydrogen sulfide, at physiological concentrations. This method should be a powerful tool for the field and enable further experimentation.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Arginine-vasopressin mediates counter-regulatory glucagon release and is diminished in type 1 diabetes

1. Angela Kim
2. Jakob G Knudsen
3. Joseph C Madara
4. Anna Benrick
5. Thomas G Hill
6. Lina Abdul Kadir
7. Joely A Kellard
8. Lisa Mellander
9. Caroline Miranda
10. Haopeng Lin
11. Timothy James
12. Kinga Suba
13. Aliya F Spigelman
14. Yanling Wu
15. Patrick E MacDonald
16. Ingrid Wernstedt Asterholm
17. Tore Magnussen
18. Mikkel Christensen
19. Tina Vilsbøll
20. Victoria Salem
21. Filip K Knop
22. Patrik Rorsman
23. Bradford B Lowell
24. Linford JB Briant

• Curated by eLife

  Evaluation Summary:

  Although the cellular and molecular mechanisms for insulin secretion regulation are relatively well defined, the precise control of glucagon secretion remains poorly understood. This paper is an elegant and thorough investigation into the role of Arginine-vasopressin (AVP) in glucagon secretion. It is known that AVP is a robust activator of calcium response in pancreatic alpha cells leading to glucagon release. The physiological relevance and regulation of this AVP-induced glucagon secretion is unclear. This manuscript goes a long way in closing this gap.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Local regulation of extracellular vesicle traffic by the synaptic endocytic machinery

1. Cassandra R. Blanchette
2. Amy L. Scalera
3. Kathryn P. Harris
4. Zechuan Zhao
5. Erica C. Dresselhaus
6. Kate Koles
7. Anna Yeh
8. Julia K. Apiki
9. Bryan A. Stewart
10. Avital A. Rodal

Reviewed by ASAPbio crowd review

Determinants shaping the nanoscale architecture of the mouse rod outer segment

1. Matthias Pöge
2. Julia Mahamid
3. Sanae S Imanishi
4. Jürgen M Plitzko
5. Krzysztof Palczewski
6. Wolfgang Baumeister

• Curated by eLife

  Evaluation Summary:

  Pöge at al. present a study of the rod outer segment (ROS). These are specialised cilia of rod photoreceptor cells, essential for sensing light cues and initiating the vision process. The authors apply cryo-FIB milling to generate highly preserved rod samples and report high-quality cryo-tomographic data providing new insights into the ultrastructure of the ROS. The work reveals potential molecular scaffolds both in the lumen of the membrane stacks and on the surface of the stack providing the structural basis for ROS crucial ordered ultrastructure. The data presented here will be highly valuable for the field of phototransduction.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Broad ultra-potent neutralization of SARS-CoV-2 variants by monoclonal antibodies specific to the tip of RBD

1. Hang Ma
2. Yingying Guo
3. Haoneng Tang
4. Chien-Te K. Tseng
5. Lei Wang
6. Huifang Zong
7. Zhenyu Wang
8. Yang He
9. Yunsong Chang
10. Shusheng Wang
11. Haiqiu Huang
12. Yong Ke
13. Yunsheng Yuan
14. Mingyuan Wu
15. Yuanyuan Zhang
16. Aleksandra Drelich
17. Kempaiah Rayavara Kempaiah
18. Bi-Hung Peng
19. Ailin Wang
20. Kaiyong Yang
21. Haiyang Yin
22. Junjun Liu
23. Yali Yue
24. Wenbo Xu
25. Shuangli Zhu
26. Tianjiao Ji
27. Xiaoju Zhang
28. Ziqi Wang
29. Gang Li
30. Guangchun Liu
31. Jingjing Song
32. Lingling Mu
33. ZongShang Xiang
34. Zhangyi Song
35. Hua Chen
36. Yanlin Bian
37. Baohong Zhang
38. Hui Chen
39. Jiawei Zhang
40. Yunji Liao
41. Li Zhang
42. Li Yang
43. Yi Chen
44. John Gilly
45. Xiaodong Xiao
46. Lei Han
47. Hua Jiang
48. Yueqing Xie
49. Qiang Zhou
50. Jianwei Zhu

Reviewed by ScreenIT