Cell Biology

A genome-wide CRISPR/Cas9 screen identifies calreticulin as a selective repressor of ATF6α

1. Joanne Tung
2. Lei Huang
3. Ginto George
4. Heather P Harding
5. David Ron
6. Adriana Ordonez

• Curated by eLife

  eLife assessment

  In this important study, the authors explore ER stress signaling mediated by ATF6 using a genome-wide gene depletion screen. They find that the ER chaperone Calreticulin binds and directly represses ATF6, a new and intriguing function for Calreticulin. The evidence presented is convincing, based on CHO genetics and biochemical analysis.

Reviewed by eLife

Phosphoproteomic analysis reveals the diversity of signaling behind ErbB inhibitor-induced phenotypes

1. Katri Vaparanta
2. Anne Jokilammi
3. Johannes Merilahti
4. Johanna Örling
5. Noora Virtanen
6. Cecilia Sahlgren
7. Klaus Elenius
8. Ilkka Paatero

Reviewed by Review Commons

Bacterial RNA promotes proteostasis through inter-tissue communication in C. elegans

1. Emmanouil Kyriakakis
2. Chiara Medde
3. Danilo Ritz
4. Geoffrey Fucile
5. Alexander Schmidt
6. Anne Spang

Reviewed by PREreview

Deep super-resolution imaging of thick tissue using structured illumination with adaptive optics

1. Jingyu Wang
2. Danail Stoychev
3. Mick Phillips
4. David Miguel Susano Pinto
5. Richard M. Parton
6. Nick Hall
7. Josh Titlow
8. Ana Rita Faria
9. Matthew Wincott
10. Dalia Gala
11. Andreas Gerondopoulos
12. Niloufer Irani
13. Ian Dobbie
14. Lothar Schermelleh
15. Martin Booth
16. Ilan Davis

Reviewed by Review Commons

Damage-induced basal epithelial cell migration modulates the spatial organization of redox signaling and sensory neuron regeneration

1. Alexandra M Fister
2. Adam Horn
3. Michael R Lasarev
4. Anna Huttenlocher

• Curated by eLife

  eLife assessment

  This important study identifies a novel link between the early keratinocyte response to wounds and the subsequent regenerative capacity of local sensory neurons. The evidence supporting the claims of the authors is convincing, although inclusion of conditional genetics or cell-autonomy tests would have strengthened the mechanistic aspects. The work will be of interest to cell and developmental biologists interested in tissue regeneration and cell interactions in a broader context.

Reviewed by eLife

Treacle’s ability to form liquid phase condensates is essential for nucleolar fibrillar center assembly, efficient rRNA transcription and processing, and rRNA gene repair

1. Artem K Velichko
2. Anastasia P Kovina
3. Artem V Luzhin
4. Nadezhda V Petrova
5. Dmitry A Deriglazov
6. Eugene P Kazakov
7. Igor I Kireev
8. Sergey V Razin
9. Omar L Kantidze

• Curated by eLife

  eLife assessment

  In this important study, the authors provide evidence that Treacle, a disease-relevant intrinsically disordered protein, undergoes biomolecular condensation to support the structure and function of the fibrillar center of the nucleolus. The findings, arising from complementary approaches, provide solid evidence for the role of Treacle condensation in supporting rDNA transcription, rRNA processing, and genome integrity. These findings may be of interest to the communities studying biomolecular condensates, nucleolar organization, and ribosome biogenesis.

Reviewed by eLife

Maturation and detoxification of synphilin-1 inclusion bodies regulated by sphingolipids

1. Xiuling Cao
2. Xiang Wu
3. Lei Zhao
4. Ju Zheng
5. Xuejiao Jin
6. Xinxin Hao
7. Joris Winderickx
8. Shenkui Liu
9. Lihua Chen
10. Beidong Liu

• Curated by eLife

  eLife assessment

  The current study sheds important light on the role of sphingolipid metabolism on the maturation of Parkinson's disease-associated Synphilin-1 inclusion bodies (SY1 IBs) on the mitochondrial surface in a yeast model using Synthetic Genetic Array (SGA) and state-of-the-art imaging techniques. The authors provide compelling evidence that downregulating the sphingolipid biosynthesis pathway leads to mitochondrial dysfunction, defective maturation, and enhanced toxicity of SY1 IBs, and this effect is conserved from yeast to mammals. Altogether, this study implicates the role of sphingolipid metabolism in the detoxification process of misfolded proteins by facilitating large IB formation on the mitochondrial outer membrane.

Reviewed by eLife

Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia

1. Yoshinobu Ichimura
2. Yuki Sugiura
3. Yoshinori Katsuragi
4. Yu-Shin Sou
5. Takefumi Uemura
6. Naoki Tamura
7. Satoko Komatsu-Hirota
8. Takashi Ueno
9. Masato Koike
10. Satoshi Waguri
11. Masaaki Komatsu

• Curated by eLife

  eLife assessment

  This paper presents useful findings on the dysmyelination phenotype of nervous system-specific Spns1 (a lysosomal lipid transporter) knockout mice. While the analysis of the phenotype is solid, the evidence for the underlying mechanisms, especially the molecular function for SPNS1, is incomplete. With more careful interpretation and/or additional experimental data, this work could have implications for understanding lipid transport and lysosomal storage diseases.

Reviewed by eLife

The ubiquitin-conjugating enzyme UBE2D/eff maintains a youthful proteome and ensures protein quality control during aging

1. Liam C Hunt
2. Kudzai Nyamkondiwa
3. Anna Stephan
4. Jianqin Jiao
5. Kanisha Kavdia
6. Vishwajeeth Pagala
7. Junmin Peng
8. Fabio Demontis

• Curated by eLife

  eLife assessment

  This valuable study presents findings on the role of the ubiquitin-conjugating enzyme UBE2D/eff in maintaining proteostasis during aging. The evidence supporting the conclusions is solid, although one reviewer had concerns about the readout for protein aggregation and the loss-of-function studies. In the future, mechanistic insights explaining the impact of UBE2D/eff deficiency on the accumulation of poly-ubiquitinated proteins and in shortening lifespan would be interesting. The present study is of broad interest to cell biologists working in aging and age-related diseases.

Reviewed by eLife

Mechanotransductive feedback control of endothelial cell motility and vascular morphogenesis

1. Devon E. Mason
2. Paula Camacho
3. Megan E. Goeckel
4. Brendan R. Tobin
5. Sebastián L. Vega
6. Pei-Hsun Wu
7. Dymonn Johnson
8. Su-Jin Heo
9. Denis Wirtz
10. Jason A. Burdick
11. Levi Wood
12. Brian Y. Chow
13. Amber N. Stratman
14. Joel D. Boerckel

• Curated by eLife

  eLife assessment

  This valuable manuscript delineates the role of YAP/TAZ-dependent transcriptional suppression in a mechanodransductive feedback loop. The evidence presented in the manuscript is generally solid. However, compared to an earlier version, some concerns remain. In particular, the in vivo validation should be strengthened, and the in vitro and in vivo models used in this work should be carefully compared in order to improve the main message of the manuscript.

Reviewed by eLife