Cell Biology

Small molecule modulator of neuronal lysosome positioning and function resolves Alzheimer’s Disease-linked pathologies in cultured human neurons

1. Amanda M. Snead
2. Sruchi Patel
3. Mia Krout
4. Ryan S. Hippman
5. Gabrielle Smith
6. Diya Dileep
7. Nitya Chagoor
8. Rachel Shi
9. Ricardo Linares
10. Andrew Dobria
11. Stephanie M. Cologna
12. Camerron Crowder
13. Leslie N. Aldrich
14. Swetha Gowrishankar

Reviewed by PREreview

TET2-mediated epigenetic modification promotes stress senescence of pancreatic β cells in type 2 diabetes mellitus

1. Weijuan Cai
2. Qingqing Song
3. Xiaoqing Mo
4. Huaqian Li
5. Yuling Song
6. Liang Yin

• Curated by eLife

  eLife Assessment

  This is a useful study that seeks to address the role of the TET family of DNA demethylation enzymes in pancreatic beta cell senescence in the context of type 2 diabetes (T2DM). Although the concepts are novel and of interest, the study presents incomplete evidence to fully support its main conclusions.

Reviewed by eLife

CCDC32 stabilizes clathrin-coated pits and drives their invagination

1. Ziyan Yang
2. Changsong Yang
3. Zheng Huang
4. Peiliu Xu
5. Yueping Li
6. Lu Han
7. Linyuan Peng
8. Xiangying Wei
9. John Pak
10. Tatyana Svitkina
11. Sandra L. Schmid
12. Zhiming Chen

Reviewed by Review Commons

Loss of ErbB3 redirects Integrin β1 from early endosomal recycling to secretion in extracellular vesicles

1. Dorival Mendes Rodrigues-Junior
2. Ana Rosa Saez-Ibanez
3. Takeshi Terabayashi
4. Nina Daubel
5. Taija Makinen
6. Olof Idevall-Hagren
7. Aristidis Moustakas
8. Ingvar Ferby

Reviewed by Review Commons

Wnt specifically induces FZD5/8 endocytosis and degradation and the involvement of RSPO-ZNRF3/RNF43 and DVL

1. Dong Luo
2. Jing Zheng
3. Shuning Lv
4. Ren Sheng
5. Maorong Chen
6. Xi He
7. Xinjun Zhang

• Curated by eLife

  eLife Assessment

  This study presents important findings demonstrating that FZD5 and FZD8, two of the ten Frizzled proteins, undergo Wnt-mediated endocytosis. The E3 ubiquitin ligases RNF43/ZNRF3 regulate their degradation in a Wnt-dependent manner, a mechanism that was not previously recognized. The evidence supporting the claims of the authors is solid. This research will be of interest to biologists specializing in Wnt signaling, cancer, and regenerative medicine.

Reviewed by eLife

Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages

1. Tomoharu Kanie
2. Roy Ng
3. Keene L Abbott
4. Niaj Mohammad Tanvir
5. Esben Lorentzen
6. Olaf Pongs
7. Peter K Jackson

• Curated by eLife

  eLife assessment

  The identification of NCS1 as a distal appendage protein that captures preciliary vesicles has important implications for understanding the early steps of ciliary assembly. Furthermore, the work has important implications for the broader understanding of NCS1, which prior to this work was focused on roles in neurotransmission, but now must be considered in a broader context. The investigators used a variety of state-of-the-art methodologies, and the conclusions are convincingly supported by the experimental data. This work will be of interest to cell biologists studying ciliary assembly, human geneticists exploring the pathology of cilia as well as neurobiologists studying NCS1.

Reviewed by eLife

Cell membrane glycan contents are biochemical factors that constitute a kinetic barrier to viral particle uptake in a protein-nonspecific manner

1. Yoshihisa Kaizuka
2. Rika Machida

• Curated by eLife

  eLife Assessment

  This fundamental work substantially advances our understanding of how the glycocalyx of cells provide a non-specific barrier for the interaction of viruses with cell-surface receptors. Using both in vitro experiments and in vivo manipulations they provide solid evidence for the properties of the glycocalyx to serve as an energy barrier as a main attribute of its mode of action. The work will be of broad interest to virologists and the cell biology community that studies host-pathogen interactions.

Reviewed by eLife

Single-molecule tracking reveals the dynamics of Ipl1 recruitment to the kinetochores and spindles in S. cerevisiae

1. Nitesh Kumar Podh
2. Ayan Das
3. Akriti Kumari
4. Kirti Garg
5. Rashmi Yadav
6. Kirti Kashyap
7. Sahil Islam
8. Anupam Gupta
9. Gunjan Mehta

Reviewed by Review Commons

Pharmacologic activation of integrated stress response kinases inhibits pathologic mitochondrial fragmentation

1. Kelsey R Baron
2. Samantha Oviedo
3. Sophia Krasny
4. Mashiat Zaman
5. Rama Aldakhlallah
6. Prerona Bora
7. Prakhyat Mathur
8. Gerald Pfeffer
9. Michael J Bollong
10. Timothy E Shutt
11. Danielle A Grotjahn
12. R Luke Wiseman

• Curated by eLife

  eLife Assessment

  This important study identifies a new class of small molecules that activate the integrated stress response (ISR) via the kinase HRI. Convincing evidence, including the image analysis pipeline, indicates that two of these compounds promote mitochondrial elongation and protect against mitochondrial fragmentation caused by chemical stress conditions or by genetic alterations. These findings open an avenue for new strategies for mitochondrial dysfunction targeting linked to ISR alterations.

Reviewed by eLife

Catalytic growth in a shared enzyme pool ensures robust control of centrosome size

1. Deb Sankar Banerjee
2. Shiladitya Banerjee

• Curated by eLife

  eLife Assessment

  This valuable work suggests a new physical model of centrosome maturation: a catalytic growth model with a shared enzyme pool. The authors provide compelling evidence to show that the model is able to reproduce various experimental results such as centrosome size scaling with cell size and centrosome growth curves in C. elegans, and that the final centrosome size is more robust to differences in initial centrosome size. While direct experimental support for this theory is currently lacking, the authors propose concrete experiments that could distinguish their shared-enzyme model from previously proposed alternatives.

Reviewed by eLife