Cell Biology

Reversing chemorefraction in colorectal cancer cells by controlling mucin secretion

1. Gerard Cantero-Recasens
2. Josune Alonso-Marañón
3. Teresa Lobo-Jarne
4. Marta Garrido
5. Mar Iglesias
6. Lluis Espinosa
7. Vivek Malhotra

• Curated by eLife

  Evaluation Summary:

  The study by Cantero-Recasens et al. aims to investigate if mucus secreted by colorectal cancers would impact the effect of the frequently used chemotherapy treatment, FOLFIRI as it has been reported that mucinous carcinomas are more treatment resistant. They further investigate the role of some mucus secretion regulatory genes in this context. The conclusions made on the effect of the mucus secretion regulatory genes are well supported, though the link to the function of mucus in reducing treatment availability needs some further clarifications.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

A novel mechanism of bulk cytoplasmic transport by cortical dynein in Drosophila ovary

1. Wen Lu
2. Margot Lakonishok
3. Anna S Serpinskaya
4. Vladimir I Gelfand

• Curated by eLife

  Evaluation Summary:

  In their manuscript, Lu et al. use a combination of experimental approaches to determine how cellular components are transported from nurse cells into the growing oocyte during Drosophila egg development. The authors demonstrate that the minus-end directed microtubule motor, dynein, generates cortical flow by gliding microtubules along the cell cortex. This flow is capable of propelling cargoes through the ring canals into the growing oocyte via a bulk cytoplasmic transport mechanism. This action is distinct from dynein's cargo transport functions, as the authors are able to replace dynein with a minus-end directed kinesin linked to the cortex and observe the same phenomenon. Overall, this work had broad significance to cell biologists and developmental biologists interested in intracellular transport functions and oocyte development.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Conjunctival epithelial cells resist productive SARS-CoV-2 infection

1. Robert M. Jackson
2. Catherine F. Hatton
3. Jarmila Stremenova Spegarova
4. Maria Georgiou
5. Joseph Collin
6. Emily Stephenson
7. Bernard Verdon
8. Iram J. Haq
9. Rafiqul Hussain
10. Jonathan M. Coxhead
11. Hardeep-Singh Mudhar
12. Bart Wagner
13. Megan Hasoon
14. Tracey Davey
15. Paul Rooney
16. C.M. Anjam Khan
17. Chris Ward
18. Malcolm Brodlie
19. Muzlifah Haniffa
20. Sophie Hambleton
21. Lyle Armstrong
22. Francisco Figueiredo
23. Rachel Queen
24. Christopher J.A. Duncan
25. Majlinda Lako

Reviewed by ScreenIT

P2Y1 purinergic receptor identified as a diabetes target in a small-molecule screen to reverse circadian β-cell failure

1. Biliana Marcheva
2. Benjamin J Weidemann
3. Akihiko Taguchi
4. Mark Perelis
5. Kathryn Moynihan Ramsey
6. Marsha V Newman
7. Yumiko Kobayashi
8. Chiaki Omura
9. Jocelyn E Manning Fox
10. Haopeng Lin
11. Patrick E Macdonald
12. Joseph Bass

• Curated by eLife

  Evaluation Summary:

  Circadian disruption is widespread in our modern 24/7 society, leading to an increased prevalence of common diseases including type 2 diabetes. The authors conducted an unbiased screen for small-molecule compounds that can restore the attenuated insulin secretion from pancreatic beta cells caused by a disrupted circadian clock. They identified ivermectin and its clock-controlled target, the P2Y1 receptor, which regulate glucose-stimulated Ca2+ influx and insulin secretion in beta cells.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Transcriptional regulation of cyclophilin D by BMP/Smad signaling and its role in osteogenic differentiation

1. Rubens Sautchuk
2. Brianna H Kalicharan
3. Katherine Escalera-Rivera
4. Jennifer H Jonason
5. George A Porter
6. Hani A Awad
7. Roman A Eliseev

• Curated by eLife

  Evaluation Summary:

  This paper is of potential interest to investigators in the bone field. The study provides evidence of CypD regulation of osteoblast differentiation and offers new insights into it's transcriptional regulation. Overall, although the findings are compelling and have the potential to advance the field, several conclusions require additional data or clarification and there are some missed opportunities to strengthen the manuscript.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Volume growth in animal cells is cell cycle dependent and shows additive fluctuations

1. Clotilde Cadart
2. Larisa Venkova
3. Matthieu Piel
4. Marco Cosentino Lagomarsino

• Curated by eLife

  Evaluation Summary:

  The regulation of cell growth is crucial for our understanding of how cells control their size as well as how they balance cell mass and volume. While recent studies carefully measured single cell mass trajectories during the cell cycle, revealing complex growth patterns, the volume growth patterns of animal cells are poorly understood. In this interesting study, Cadart et al. now present high-precision measurements of 1700 HeLa cell growth trajectories and offering evidence for the mechanisms that regulate volume growth-rate fluctuations. This is an important demonstration of cell autonomous volume regulation.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Activation of transient receptor potential vanilloid 4 is involved in pressure overload-induced cardiac hypertrophy

1. Yan Zou
2. Miaomiao Zhang
3. Qiongfeng Wu
4. Ning Zhao
5. Minwei Chen
6. Cui Yang
7. Yimei Du
8. Bing Han

• Curated by eLife

  Evaluation Summary:

  This study investigated the role of transient receptor potential vanilloid 4 (TRPV4) in pressure overload-induced cardiac hypertrophy. The expression of TPRV4 is increased in both heart failure and pressure overload-induced cardiac hypertrophy models. The TRPV4 deletion or inhibition ameliorated the hypertrophy cardiac pathology. The authors propose that TRPV4 is a potential therapeutic target for cardiac hypertrophy.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Plasma membrane damage limits replicative lifespan in yeast and induces premature senescence in human fibroblasts

1. Kojiro Suda
2. Yohsuke Moriyama
3. Nurhanani Razali
4. Yatzu Chiu
5. Yumiko Masukagami
6. Koutarou Nishimura
7. Hunter Barbee
8. Hiroshi Takase
9. Shinju Sugiyama
10. Yuta Yamazaki
11. Yoshikatsu Sato
12. Tetsuya Higashiyama
13. Yoshikazu Johmura
14. Makoto Nakanishi
15. Keiko Kono

Reviewed by Review Commons

Autophagosomes fuse to phagosomes and facilitate the degradation of apoptotic cells in Caenorhabditis elegans

1. Omar Peña-Ramos
2. Lucia Chiao
3. Xianghua Liu
4. Xiaomeng Yu
5. Tianyou Yao
6. Henry He
7. Zheng Zhou

• Curated by eLife

  Evaluation Summary:

  This study presents evidence that autophagosomes fuse with phagosomes and that this promotes the degradation of phagocytosed cell corpses. The study also resolves controversy in the field about the question why genes involved in autophagy affect cell corpse engulfment and degradation. With some additional data to solidify the main conclusions, the work will be of interest to a broad cell biology audience.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

The GARP complex prevents sterol accumulation at the trans-Golgi network during dendrite remodeling

1. Caitlin E. O’Brien
2. Susan H. Younger
3. Lily Yeh Jan
4. Yuh Nung Jan

Reviewed by Review Commons