Cell Biology

Structural basis for cytoplasmic dynein-1 regulation by Lis1

1. John P Gillies
2. Janice M Reimer
3. Eva P Karasmanis
4. Indrajit Lahiri
5. Zaw Min Htet
6. Andres E Leschziner
7. Samara L Reck-Peterson

• Curated by eLife

  Evaluation Summary:

  LIS1 is a key regulator of the microtubule motor cytoplasmic dynein. Here the authors use yeast proteins and streptavidin-coated grids to solve the first high-resolution (3.1Å) structure of the dynein-Lis1 complex. The two beta-propellors in a Lis1 dimer make contact with different sites on a single dynein motor domain. Mutagenesis shows both sites are important for yeast and human dynein and uncover how they modulate motor function.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Load adaptation of endocytic actin networks

1. Charlotte Kaplan
2. Sam J. Kenny
3. Xuyan Chen
4. Johannes Schöneberg
5. Ewa Sitarska
6. Alba Diz-Muñoz
7. Matthew Akamatsu
8. Ke Xu
9. David G. Drubin

Reviewed by ASAPbio crowd review

Quantification of nuclear transport inhibition by SARS-CoV-2 ORF6 using a broadly applicable live-cell dose-response pipeline

1. Tae Yeon Yoo
2. Timothy Mitchison

Reviewed by ScreenIT

Single-cell imaging of ERK and Akt activation dynamics and heterogeneity induced by G-protein-coupled receptors

1. Sergei Chavez-Abiega
2. Max L. B. Grönloh
3. Theodorus W. J. Gadella
4. Frank J. Bruggeman
5. Joachim Goedhart

Reviewed by Review Commons

A phosphoinositide and RAB switch controls early macropinocytosis

1. Hélène Spangenberg
2. Marte Sneeggen
3. Maria Mateo Tortola
4. Camila Valenzuela
5. Yuen-Yan Chang
6. Harald Stenmark
7. Camilla Raiborg
8. Kay Oliver Schink

• Curated by eLife

  Evaluation Summary:

  This study is of interest to the broader audience of cell biologists, as it aims to dissect the hierarchy of protein recruitment and lipid conversion events that may provide a potential mechanism for the formation, maturation and fate of macropinosomes. The conclusions are based on the observation that pharmacological inhibition of the lipid kinase VPS34, which generates the signalling lipid PI3P on endosomes, prevents accumulation of Rab5 on macropinosomes, blocking their maturation and causing them to re-fuse with the plasma membrane.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Chlamydomonas ARMC2/PF27 is an obligate cargo adapter for intraflagellar transport of radial spokes

1. Karl F Lechtreck
2. Yi Liu
3. Jin Dai
4. Rama A Alkhofash
5. Jack Butler
6. Lea Alford
7. Pinfen Yang

• Curated by eLife

  Evaluation Summary:

  This paper is of broad interest to readers interested in motile cilia and cargo transport mediated by intraflagellar transport (IFT). It examines how radial spokes are trafficked into cilia by IFT, which represents a key process in the assembly of motile cilia. The authors demonstrate that an adaptor protein (ARMC2) is needed for association of radial spokes with the IFT machinery. They also find that the interaction of ARMC2/radial spokes with individual IFT trains (or particles) occurs in a stochastic manner rather than there being a specialized subset of trains specifically designated for a particular cargo. The results support the key claims in the paper.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Decoupling SARS-CoV-2 ORF6 localization and interferon antagonism

1. Hoi Tong Wong
2. Victoria Cheung
3. Daniel J. Salamango

Reviewed by ScreenIT

A genetic compensatory mechanism regulated by Jun and Mef2d modulates the expression of distinct class IIa Hdacs to ensure peripheral nerve myelination and repair

1. Sergio Velasco-Aviles
2. Nikiben Patel
3. Angeles Casillas-Bajo
4. Laura Frutos-Rincón
5. Enrique Velasco
6. Juana Gallar
7. Peter Arthur-Farraj
8. Jose A Gomez-Sanchez
9. Hugo Cabedo

• Curated by eLife

  Evaluation Summary:

  This study is of interest to scientists working in the field of genetic control of glial cell differentiation, myelination and repair. The data are extensive, of high quality, support their main conclusions, and provide novel insights into regulation of genetic compensatory mechanisms. The presentation and interpretation of the data can be improved.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Essential role of hyperacetylated microtubules in innate immunity escape orchestrated by the EBV-encoded BHRF1 protein

1. Damien Glon
2. Géraldine Vilmen
3. Daniel Perdiz
4. Eva Hernandez
5. Guillaume Beauclair
6. Frédérique Quignon
7. Clarisse Berlioz-Torrent
8. Vincent Maréchal
9. Christian Poüs
10. Marion Lussignol
11. Audrey Esclatine

Reviewed by Review Commons

ACE2 expression and localization are regulated by CFTR: implications beyond cystic fibrosis

1. Valentino Bezzerri
2. Valentina Gentili
3. Martina Api
4. Alessia Finotti
5. Chiara Papi
6. Anna Tamanini
7. Debora Olioso
8. Martina Duca
9. Erika Tedesco
10. Sara Leo
11. Monica Borgatti
12. Sonia Volpi
13. Paolo Pinton
14. Giulio Cabrini
15. Roberto Gambari
16. Francesco Blasi
17. Giuseppe Lippi
18. Alessandro Rimessi
19. Roberta Rizzo
20. Marco Cipolli

Reviewed by ScreenIT