Cell Biology

TUBA1A tubulinopathy mutants disrupt neuron morphogenesis and override XMAP215/Stu2 regulation of microtubule dynamics

1. Katelyn J Hoff
2. Jayne E Aiken
3. Mark A Gutierrez
4. Santos J Franco
5. Jeffrey K Moore

• Curated by eLife

  Evaluation Summary:

  Tubulin mutations underlie a number of neurodevelopmental diseases, but their effect on microtubule function remains largely unknown. Using a combination of approaches and model systems, the authors provide evidence that the disease-associated alpha-tubulin mutations V409A and V409I may perturb microtubule polymerization. In addition, the mutations in alpha tubulin directly impact Tumor Overexpressed Gene domain proteins from recruiting tubulins to regulate microtubule dynamics. This study demonstrates a link between regulators of microtubule dynamics and disease pathogenesis. It will be of interest to the cytoskeleton and neurobiology fields.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Tom70-based transcriptional regulation of mitochondrial biogenesis and aging

1. Qingqing Liu
2. Catherine E Chang
3. Alexandra C Wooldredge
4. Benjamin Fong
5. Brian K Kennedy
6. Chuankai Zhou

• Curated by eLife

  Evaluation Summary:

  The authors test the hypothesis that components of the TOM complex regulate efficient mitochondrial biogenesis by coordinating the synthesis (via controlling transcription of the corresponding RNAs) of mitochondrial proteins with the rate of mitochondrial protein import. It has previously been established that failure to import mitochondrial proteins results in the accumulation of toxic protein aggregates in the cytosol. The authors conclude that Tom70 fulfills this role and find that Tom70 expression declines as cells age, which contributes to age-associated mitochondrial dysfunction.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

The roles of distinct Ca ²⁺ signaling mediated by Piezo and inositol triphosphate receptor (IP3R) in the remodeling of E-cadherin during cell dissemination

1. Alejandra J.H. Cabrera
2. Barry M. Gumbiner
3. Young V. Kwon

Reviewed by Review Commons, preLights

Adult mouse fibroblasts retain organ-specific transcriptomic identity

1. Elvira Forte
2. Mirana Ramialison
3. Hieu T Nim
4. Madison Mara
5. Jacky Y Li
6. Rachel Cohn
7. Sandra L Daigle
8. Sarah Boyd
9. Edouard G Stanley
10. Andrew G Elefanty
11. John Travis Hinson
12. Mauro W Costa
13. Nadia A Rosenthal
14. Milena B Furtado

• Curated by eLife

  Evaluation Summary:

  This paper is of general interest to a broad audience of scientists working in regenerative medicine, tissue engineering, disease modelling, and stem cell fields. It reveals organ fibroblast heterogeneity and shows that the organ-specific identity is preserved in vitro and during ectopic transplantation in vivo. The right choice of fibroblasts might therefore be critical in the fields mentioned above.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Comparative analysis of cell–cell communication at single-cell resolution

1. Aaron J. Wilk
2. Alex K. Shalek
3. Susan Holmes
4. Catherine A. Blish

Reviewed by ScreenIT

Centrioles generate a local pulse of Polo/PLK1 activity to initiate mitotic centrosome assembly

1. Siu‐Shing Wong
2. Zachary M Wilmott
3. Saroj Saurya
4. Ines Alvarez‐Rodrigo
5. Felix Y Zhou
6. Kwai‐Yin Chau
7. Alain Goriely
8. Jordan W Raff

Reviewed by Review Commons

USP22 controls type III interferon signaling and SARS-CoV-2 infection through activation of STING

1. Rebekka Karlowitz
2. Megan L. Stanifer
3. Jens Roedig
4. Geoffroy Andrieux
5. Denisa Bojkova
6. Marco Bechtel
7. Sonja Smith
8. Lisa Kowald
9. Ralf Schubert
10. Melanie Boerries
11. Jindrich Cinatl
12. Steeve Boulant
13. Sjoerd J. L. van Wijk

Reviewed by ScreenIT

Nanoscale architecture and coordination of actin cores within the sealing zone of human osteoclasts

1. Marion Portes
2. Thomas Mangeat
3. Natacha Escallier
4. Ophélie Dufrancais
5. Brigitte Raynaud-Messina
6. Christophe Thibault
7. Isabelle Maridonneau-Parini
8. Christel Vérollet
9. Renaud Poincloux

• Curated by eLife

  Evaluation Summary:

  In this manuscript, the authors studied, using different types of microscopy, the structure and function of podosomes in primary human osteoclasts, providing interesting new insights into the organization and dynamics of the osteoclast sealing zone. The authors describe the general structure of the actin rings of osteoclasts and explore the dynamics of actin and associated proteins in the actin ring, proposing a model in which the function of podosomes during bone resorption is accomplished through the coordination of islets of the actin core and not through the global coordination of all podosome subunits that form the sealing zone. This article has the potential to generate a significant impact in the field of osteoclast biology. Notably, this study could also expand the understanding of matrix degradation/invasion in other tissues.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Quantitative mapping of keratin networks in 3D

1. Reinhard Windoffer
2. Nicole Schwarz
3. Sungjun Yoon
4. Teodora Piskova
5. Michael Scholkemper
6. Johannes Stegmaier
7. Andrea Bönsch
8. Jacopo Di Russo
9. Rudolf E Leube

• Curated by eLife

  Evaluation Summary:

  Windoffer et al. developed an image processing platform to quantify the 3D network of keratin filaments. The concept of this approach is based on 3D visualization of fluorescently labeled proteins using confocal scanning microscopy. The major advantage of this approach is that after initial segmentation of the network, filaments are divided into pieces for detailed analyses in silico. This approach allows for quantification of the segmented polymer and compute some of the network properties of the keratin filaments in cells, in cultured cells ex vivo and specific cell types in situ. Additionally, this approach allows nice visualization of the keratin network in 3D. The resulting contribution is original, provides insight at both a methodological and biological levels, and extends emerging information about the high resolution structure of intermediate filaments in situ using cryoelectron tomography.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors. Reviewer #1 and Reviewer #3 opted to remain anonymous to the authors.)

Reviewed by eLife

The AAA‐ATPase ATAD1 and its partners promote degradation of desmin intermediate filaments in muscle

1. Dina Aweida
2. Shenhav Cohen

Reviewed by Review Commons