Cell Biology

Effect of COVID-19 mRNA Vaccine on Human Lung Carcinoma Cells In Vitro by Means of Raman Spectroscopy and Imaging

1. Halina Abramczyk
2. Jakub Surmacki

Reviewed by ScreenIT

Glycogen Synthase Kinase-3 Interaction Domain Enhances Phosphorylation of SARS-CoV-2 Nucleocapsid Protein

1. Jun Seop Yun
2. Hyeeun Song
3. Nam Hee Kim
4. So Young Cha
5. Kyu Ho Hwang
6. Jae Eun Lee
7. Cheol-Hee Jeong
8. Sang Hyun Song
9. Seonghun Kim
10. Eunae Sandra Cho
11. Hyun Sil Kim
12. Jong In Yook

Reviewed by ScreenIT

Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cells

1. Koji Kikuchi
2. Yasuhisa Sakamoto
3. Akiyoshi Uezu
4. Hideyuki Yamamoto
5. Kei-ichiro Ishiguro
6. Kenji Shimamura
7. Taro Saito
8. Shin-ichi Hisanaga
9. Hiroyuki Nakanishi

Reviewed by Review Commons

Eco1-dependent cohesin acetylation anchors chromatin loops and cohesion to define functional meiotic chromosome domains

1. Rachael E Barton
2. Lucia F Massari
3. Daniel Robertson
4. Adèle L Marston

• Curated by eLife

  Evaluation Summary:

  The cohesin complex is involved in both sister chromatid cohesion (SCC) and intra-chromatid loop formation. Combining molecular genetic and cytological tools with genome-wide calibrated ChIP and HiC analyses in budding yeast, the authors elegantly show that Eco1 and Eco1-mediated Smc3 acetylation promote the boundary formation of chromatin loops by cohesin, which is critical for both meiotic recombination in prophase I and sister chromatid segregation in meiosis II. Cohesin's role in the boundary formation is independent of meiotic DNA replication and of antagonizing a cohesion releasing protein, Wapl. Future studies will reveal the molecular mechanisms of how Eco1-mediated Smc3 acetylation stabilizes cohesin at convergent transcription sites for boundary formation.

  This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Comprehensive Evaluation of ACE2-Fc Combination with Neutralization Antibody on Broad Protection against SARS-CoV-2 and Its Variants

1. Haoneng Tang
2. Yong Ke
3. Hang Ma
4. Lei Han
5. Lei Wang
6. Huifang Zong
7. Yunsheng Yuan
8. Zhenyu Wang
9. Yang He
10. Yunsong Chang
11. Shusheng Wang
12. Junjun Liu
13. Yali Yue
14. Wenbo Xu
15. Xiaoju Zhang
16. Ziqi Wang
17. Li Yang
18. Hua Chen
19. Yanlin Bian
20. Baohong Zhang
21. Yunji Liao
22. Haiyang Yin
23. Yi Chen
24. En Zhang
25. Xiaoxiao Zhang
26. Hua Jiang
27. Yueqing Xie
28. John Gilly
29. Mingyuan Wu
30. Tao Sun
31. Jianwei Zhu

Reviewed by ScreenIT

ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1

1. Boris Simonetti
2. James L. Daly
3. Lorena Simón-Gracia
4. Katja Klein
5. Saroja Weeratunga
6. Carlos Antón-Plágaro
7. Allan Tobi
8. Lorna Hodgson
9. Philip A. Lewis
10. Kate J. Heesom
11. Deborah K. Shoemark
12. Andrew D. Davidson
13. Brett M. Collins
14. Tambet Teesalu
15. Yohei Yamauchi
16. Peter J. Cullen

Reviewed by ScreenIT

Differentiation of mouse embryonic stem cells into cells with spermatogonia-like morphology with chemical intervention-dependent increased gene expression of LIM homeobox 1 (Lhx1)

1. Cameron Moshfegh
2. Sebastian G. Rambow
3. Seraina A. Domenig
4. Aldona Pieńkowska-Schelling
5. Ulrich Bleul
6. Viola Vogel

Reviewed by PeerRef

Leading edge maintenance in migrating cells is an emergent property of branched actin network growth

1. Rikki M Garner
2. Julie A Theriot

• Curated by eLife

  Evaluation Summary:

  This paper describes analysis and modeling of leading edge fluctuations in migrating cells driven by a branched Arp2/3 lamellipodial network. A stochastic model shows how branching contributes to shape stability, and reproduces the measured spectrum and dynamics of leading edge fluctuations. Analysis of the model as a function of branching angle suggests that the Arp2/3 branching angle might be selected to smooth lamellipodial shape. The authors provide new ideas to a big field of research, including Fourier analysis of leading edge fluctuations, which is a novel approach. The modeling methods and model design seem valid and the paper is well written.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Cortical waves mediate the cellular response to electric fields

1. Qixin Yang
2. Yuchuan Miao
3. Leonard J Campanello
4. Matt J Hourwitz
5. Bedri Abubaker-Sharif
6. Abby L Bull
7. Peter N Devreotes
8. John T Fourkas
9. Wolfgang Losert

• Curated by eLife

  Evaluation Summary:

  This paper is of interest for cell biologists and biophysicists that work on eukaryotic cell motility and the dynamics of the actin cytoskeleton. The authors combine a series of clever biological approaches to fuse small Dictyostelium cells together into 'giant cells' that make it much easier to spatially resolve actin wave dynamics with and without electrical stimulation when cultured on smooth or nano-textured surfaces. Sophisticated and methodical computational approaches are used to analyze these images and relate the data to actin polymerization and wave dynamics parameters using optic flow and associated techniques. This study is mostly descriptive, a full mechanistic explanation of the results remains open, but this compelling experimental system opens up possibilities for the field to analyze the molecular subtleties involved in these cytoskeletal reorganizations.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Three-dimensional structure of kinetochore-fibers in human mitotic spindles

1. Robert Kiewisz
2. Gunar Fabig
3. William Conway
4. Daniel Baum
5. Daniel Needleman
6. Thomas Müller-Reichert

• Curated by eLife

  Evaluation Summary:

  Kiewisz and colleagues performed sophisticated reconstructions of kinetochore-fibers within human spindles using electron tomography, and then analyzed the ultrastructure and organization of their microtubules. This work will not only serve as an incredible resource for the field, but has clear implications for models of kinetochore-fiber and spindle self-organization.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife