A dual-receptor mechanism between integrins and ACE2 widens SARS-CoV-2 tissue tropism

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1. 

   ScreenIT

   Jan 6, 2022

   SciScore for 10.1101/2022.01.02.474028: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   NIH rigor criteria are not applicable to paper type.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   In vitro assessment of Cilengitide inhibition of SARS-CoV-2 binding: An in vitro para-nitrophenyl phosphate binding assay was performed using human colorectal adenocarcinoma Caco-2 cells and formaldehyde-fixed SARS-CoV-2 to investigate the ability of RGD cyclic pentapeptide compound Cilengitide (0.05 and 0.005 μM) to reduce viral adherence, as described previously18.	Caco-2 suggested: None
   Software and Algorithms
   Sentences	Resources
   Structures were energetically repaired in Yasara using the FoldX suite and visually compared using PyMol and CCG Molecular Operating Environment (MOE).	FoldX s…

   SciScore for 10.1101/2022.01.02.474028: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   NIH rigor criteria are not applicable to paper type.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   In vitro assessment of Cilengitide inhibition of SARS-CoV-2 binding: An in vitro para-nitrophenyl phosphate binding assay was performed using human colorectal adenocarcinoma Caco-2 cells and formaldehyde-fixed SARS-CoV-2 to investigate the ability of RGD cyclic pentapeptide compound Cilengitide (0.05 and 0.005 μM) to reduce viral adherence, as described previously18.	Caco-2 suggested: None
   Software and Algorithms
   Sentences	Resources
   Structures were energetically repaired in Yasara using the FoldX suite and visually compared using PyMol and CCG Molecular Operating Environment (MOE).	FoldX suggested: (FoldX, RRID:SCR_008522) PyMol suggested: (PyMOL, RRID:SCR_000305)
   Only the best ranked binding pose obtained from AutoDock Vina was used for MD simulations.	AutoDock suggested: (AutoDock, RRID:SCR_012746)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • No funding statement was detected.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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   Read the original source
2. 

   Version published to 10.1101/2022.01.02.474028v1 on bioRxiv

   Jan 3, 2022