Cell Biology

An improved organ explant culture method reveals stem cell lineage dynamics in the adult Drosophila intestine

1. Marco Marchetti
2. Chenge Zhang
3. Bruce A Edgar

• Curated by eLife

  Evaluation Summary:

  Marchetti and colleagues present a promising, ex vivo culture method for the Drosophila adult midgut and other abdominal organs. There are numerous advantages of the authors' method that will attract broad interest and enable real-time analysis of new and important scientific questions. There are concerns about the authors' interpretations of asymmetric/symmetric fate outcomes and terminal differentiation, more information about midgut viability is needed, and comparison of ex vivo vs in vivo regeneration would be useful.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Dual Inhibition of Vacuolar-ATPase and TMPRSS2 Is Required for Complete Blockade of SARS-CoV-2 Entry into Cells

1. Simoun Icho
2. Edurne Rujas
3. Krithika Muthuraman
4. John Tam
5. Huazhu Liang
6. Shelby Landreth
7. Mingmin Liao
8. Darryl Falzarano
9. Jean-Philippe Julien
10. Roman A. Melnyk

Reviewed by ScreenIT

Measurements and simulations of microtubule growth imply strong longitudinal interactions and reveal a role for GDP on the elongating end

1. Joseph M Cleary
2. Tae Kim
3. Annan SI Cook
4. Lauren A McCormick
5. William O Hancock
6. Luke M Rice

• Curated by eLife

  Evaluation Summary:

  The authors use interference reflection microscopy to image a growing microtubule for long intervals at high frame rates, overcoming a limitation of fluorescence. Using careful quantitative analysis, the authors find that the kinetics of dynamic instability are "slow", in contrast to the "rapid kinetics" previously reported. This work provides new mechanistic insight into the mechanism of microtubule growth and is of interest to biologists and physicists interested in cytoskeletal filament dynamics.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Contribution of Trp63CreERT2-labeled cells to alveolar regeneration is independent of tuft cells

1. Huachao Huang
2. Yinshan Fang
3. Ming Jiang
4. Yihan Zhang
5. Jana Biermann
6. Johannes C Melms
7. Jennifer A Danielsson
8. Ying Yang
9. Li Qiang
10. Jia Liu
11. Yiwu Zhou
12. Manli Wang
13. Zhihong Hu
14. Timothy C Wang
15. Anjali Saqi
16. Jie Sun
17. Ichiro Matsumoto
18. Wellington V Cardoso
19. Charles W Emala
20. Jian Zhu
21. Benjamin Izar
22. Hongmei Mou
23. Jianwen Que

• Curated by eLife

  Evaluation Summary:

  This study addresses an interesting question in lung regeneration following viral injury. The authors describe the ectopic differentiation of tuft cells that were derived from lineage-tagged p63+ cells post influenza virus infection. These tuft cells do not appear to proliferate or give rise to other lineages. Importantly, they demonstrate that depletion of tuft cells caused by genetic deletion of Pou2f3 in p63+ cells has no effect on the expansion or resolution of Krt5+ pods after infection, implying that tuft cells play no functional role in this process.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife, ScreenIT

Genome-wide functional analysis reveals key roles for kinesins in the mammalian and mosquito stages of the malaria parasite life cycle

1. Mohammad Zeeshan
2. Ravish Rashpa
3. David J. P. Ferguson
4. Steven Abel
5. Zeinab Chahine
6. Declan Brady
7. Sue Vaughan
8. Carolyn A. Moores
9. Karine G. Le Roch
10. Mathieu Brochet
11. Anthony A. Holder
12. Rita Tewari

Reviewed by preLights, Review Commons

Centriole distal-end proteins CP110 and Cep97 influence centriole cartwheel growth at the proximal end

1. Mustafa G. Aydogan
2. Laura E. Hankins
3. Thomas L. Steinacker
4. Mohammad Mofatteh
5. Saroj Saurya
6. Alan Wainman
7. Siu-Shing Wong
8. Xin Lu
9. Felix Y. Zhou
10. Jordan W. Raff

Reviewed by Review Commons

DNA circles promote yeast ageing in part through stimulating the reorganization of nuclear pore complexes

1. Anne C Meinema
2. Anna Marzelliusardottir
3. Mihailo Mirkovic
4. Théo Aspert
5. Sung Sik Lee
6. Gilles Charvin
7. Yves Barral

• Curated by eLife

  Evaluation Summary:

  This interesting study examines a potential relationship between the tethering of extrachromosomal DNA (ecDNA) to the nuclear pore complex (NPC) and its role in aging; a model is proposed whereby the nuclear basket is displaced by ecDNA anchoring, which leads to a broader remodeling of the NPC that is distinct from NPC damage. This idea is conceptually novel and will represent an important advance, although some more support for the conclusions is still needed.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Identification of a Golgi-localized peptide reveals a minimal Golgi-targeting motif

1. Alexandra P. Navarro
2. Iain M. Cheeseman

Reviewed by Review Commons

Determination of oligomeric states of proteins via dual-color colocalization with single molecule localization microscopy

1. Hua Leonhard Tan
2. Stefanie Bungert-Plümke
3. Daniel Kortzak
4. Christoph Fahlke
5. Gabriel Stölting

• Curated by eLife

  Evaluation Summary:

  The authors present a method for measuring the oligomerisation state of tagged membrane proteins by PALM co-localisation that is new, interesting and potentially very useful for identifying oligomerization states of unknown proteins in native cells. While the authors develop a basic theory and apply the method to a set of candidate proteins with solid results, their implementation could be refined and improved, which would help to better delineate the full scope and the limitations of their method. An open-source software tool would help other researchers to adopt this analysis. The work is relevant for cell biologists, especially those studying membrane proteins.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Repression of hypoxia-inducible factor-1 contributes to increased mitochondrial reactive oxygen species production in diabetes

1. Xiaowei Zheng
2. Sampath Narayanan
3. Cheng Xu
4. Sofie Eliasson Angelstig
5. Jacob Grünler
6. Allan Zhao
7. Alessandro Di Toro
8. Luciano Bernardi
9. Massimiliano Mazzone
10. Peter Carmeliet
11. Marianna Del Sole
12. Giancarlo Solaini
13. Elisabete A Forsberg
14. Ao Zhang
15. Kerstin Brismar
16. Tomas A Schiffer
17. Neda Rajamand Ekberg
18. Ileana Ruxandra Botusan
19. Fredrik Palm
20. Sergiu-Bogdan Catrina

• Curated by eLife

  Evaluation Summary:

  The authors report that hyperglicemia suppresses HIF1a activity in models of diabetic nephropathy, which in turn leads to cell death by increasing mitochondrial ROS. The notion that HIF1a regulates mitochondrial respiration and thus mitochondrial ROS production is well documented in numerous systems. However, this model has not been tested in the context of diabetic nephropathy so far. The study is thus novel, informative and with interesting translational implications.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife