1. Structural and functional properties of a plant NRAMP-related aluminum transporter

    This article has 8 authors:
    1. Karthik Ramanadane
    2. Márton Liziczai
    3. Dragana Markovic
    4. Monique S Straub
    5. Gian T Rosalen
    6. Anto Udovcic
    7. Raimund Dutzler
    8. Cristina Manatschal
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study provides valuable insight into the diversity within the NRAMP superfamily of transporters. Evidence of divalent metal ion transport and the structure (obtained without added metal ions) are convincing. However, molecular insight into Al3+ recognition and transport is incomplete, and the work would be strengthened by the determination of a metal-bound structure or additional experiments (such as molecular dynamics simulations or quantitative Al3+ binding/transport assays) to support the proposed Al3+ binding site. The work will be of interest to structural biologists and biophysicists studying NRAMP transporters.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  2. Zinc activation of OTOP proton channels identifies structural elements of the gating apparatus

    This article has 6 authors:
    1. Bochuan Teng
    2. Joshua P Kaplan
    3. Ziyu Liang
    4. Kevin Saejin Chyung
    5. Marcel P Goldschen-Ohm
    6. Emily R Liman
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This valuable study discovers that zinc ions can activate some OTOP proton channels, identifying a pharmacological tool for research, and further establishing that OTOP channels gate. The data presented provide convincing support for the conclusions made by the authors, and the study is expected to be of considerable interest to physiologists investigating OTOP and other proton channels.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  3. A pH-dependent cluster of charges in a conserved cryptic pocket on flaviviral envelopes

    This article has 5 authors:
    1. Lorena Zuzic
    2. Jan K Marzinek
    3. Ganesh S Anand
    4. Jim Warwicker
    5. Peter J Bond
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The study represents an impressive effort to use atomistic simulations to probe cryptic binding sites in the envelope of six flaviviruses. Moreover, using constant pH simulations, the authors suggest that a cluster of ionizable residues contribute to the pH dependent conformational rearrangements required in the infection process. Therefore, the study provides new mechanistic insights that can be helpful in future efforts to develop drugs that target flaviviruses.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  4. PAR recognition by PARP1 regulates DNA‐dependent activities and independently stimulates catalytic activity of PARP1

    This article has 3 authors:
    1. Waghela Deeksha
    2. Suman Abhishek
    3. Eerappa Rajakumara

    Reviewed by Review Commons

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  5. Room-temperature crystallography reveals altered binding of small-molecule fragments to PTP1B

    This article has 13 authors:
    1. Tamar Skaist Mehlman
    2. Justin T Biel
    3. Syeda Maryam Azeem
    4. Elliot R Nelson
    5. Sakib Hossain
    6. Louise Dunnett
    7. Neil G Paterson
    8. Alice Douangamath
    9. Romain Talon
    10. Danny Axford
    11. Helen Orins
    12. Frank von Delft
    13. Daniel A Keedy
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      Based on two room-temperature X-ray crystallographic screens of PTP1B phosphatase against two sets of chemical fragments, and by comparing the results from a previous cryo screen, the authors report the important observation that, in addition to overlapping but non-identical sets of hits compared to the cryo screen, the room-temperature screens lead to significant differences in terms of binding sites and poses for some of the hits. The study provides compelling support for the use of room-temperature X-ray crystallography in early-stage drug discovery and highlights that temperature should be used as a parameter in efforts to extract additional insight from such analyses.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  6. Timeline of changes in spike conformational dynamics in emergent SARS-CoV-2 variants reveal progressive stabilization of trimer stalk with altered NTD dynamics

    This article has 6 authors:
    1. Sean M Braet
    2. Theresa SC Buckley
    3. Varun Venkatakrishnan
    4. Kim-Marie A Dam
    5. Pamela J Bjorkman
    6. Ganesh S Anand
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This fundamental and timely study provides insights into the structural dynamics of several relevant mutant forms of SARS-CoV-2 spike protein, including the most recent omicron variant. The hydrogen/deuterium-exchange studies provide compelling evidence for the stabilization of the spike stalk in conjunction with increased dynamics of the N-terminal domain, where binding to the ACE2 receptor occurs. These results have profound implications for the development of small molecule inhibitors of the spike protein-ACE2 receptor interaction.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  7. DNAJA2 and Hero11 mediate similar conformational extension and aggregation suppression of TDP-43

    This article has 4 authors:
    1. Andy Y.W. Lam
    2. Kotaro Tsuboyama
    3. Hisashi Tadakuma
    4. Yukihide Tomari

    Reviewed by Review Commons

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  8. Gating interactions steer loop conformational changes in the active site of the L1 metallo-β-lactamase

    This article has 11 authors:
    1. Zhuoran Zhao
    2. Xiayu Shen
    3. Shuang Chen
    4. Jing Gu
    5. Haun Wang
    6. Maria F Mojica
    7. Moumita Samanta
    8. Debsindhu Bhowmik
    9. Alejandro J Vila
    10. Robert A Bonomo
    11. Shozeb Haider
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      In this useful study, the authors utilize state-of-the-art computational methods complemented with some experimental validation to investigate the dynamics of flexible loops of the L1 Metallo-β-lactamase enzyme, resulting in a better understanding of the various conformational states useful for the rational design of superior β-lactamase inhibitors/antibiotics. The evidence supporting the claims is solid, and the work will be of interest to computational, experimental biologists, and drug designers working on antibiotic resistance.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  9. The SARS-CoV-2 accessory protein Orf3a is not an ion channel, but does interact with trafficking proteins

    This article has 14 authors:
    1. Alexandria N Miller
    2. Patrick R Houlihan
    3. Ella Matamala
    4. Deny Cabezas-Bratesco
    5. Gi Young Lee
    6. Ben Cristofori-Armstrong
    7. Tanya L Dilan
    8. Silvia Sanchez-Martinez
    9. Doreen Matthies
    10. Rui Yan
    11. Zhiheng Yu
    12. Dejian Ren
    13. Sebastian E Brauchi
    14. David E Clapham
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The function of specific proteins made by SARS-CoV-1 and SARS-CoV-2 is under debate, with diverging claims previously published regarding the ability of Orf3a proteins from either virus to form ion channels. The authors undertook a thorough characterization of Orf3a from CoV-1 and CoV-2 by combining data from a range of different structural and functional experiments, arguably providing the most compelling evidence to date that Orf3a from viruses is not an ion channel. Instead, the orthologue-specific interaction with a component of a larger protein complex suggests a role of one of the two membrane proteins in the endo-lysosomal pathway. The work is significant from a fundamental science perspective, for its implications for COVID antiviral development strategies, and also for establishing guidelines for future identification of true viral ion channels.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  10. Drug specificity and affinity are encoded in the probability of cryptic pocket opening in myosin motor domains

    This article has 10 authors:
    1. Artur Meller
    2. Jeffrey M Lotthammer
    3. Louis G Smith
    4. Borna Novak
    5. Lindsey A Lee
    6. Catherine C Kuhn
    7. Lina Greenberg
    8. Leslie A Leinwand
    9. Michael J Greenberg
    10. Gregory R Bowman
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important study presents insights into how conformational dynamics differentially influences drug specificity and affinity in myosin isoforms using computational approaches. The evidence supporting the conclusions is convincing, establishing a relationship between inhibition and protein dynamics using state of the art computational techniques followed by experimental validation. The work will be of broad interest to computational biophysicists and medicinal chemists.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
Previous Page 34 of 90 Next