1. Protein compactness and interaction valency define the architecture of a biomolecular condensate across scales

    This article has 5 authors:
    1. Anton A Polyansky
    2. Laura D Gallego
    3. Roman G Efremov
    4. Alwin Köhler
    5. Bojan Zagrovic
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study reports a joint experimental and computational investigation of the structural features of biomolecular condensates formed by a specific intrinsically disordered protein. The authors also adapt emerging rules to discuss and physico-chemical determinants of these structures of condensates. Specifically, the authors adapt the analysis of fractal structures, co-opted from the field of colloidal chemistry / physics, and generate important insights regarding the network-like organization of disordered proteins within in silico facsimiles of condensates. The usage of these analyses in the context of studying all atom models for multi-chain assemblies intended to mimic the internal organization of condensates is very interesting. The work is of relevance to cell biology and structural biology.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  2. Robust membrane protein tweezers reveal the folding speed limit of helical membrane proteins

    This article has 4 authors:
    1. Seoyoon Kim
    2. Daehyo Lee
    3. WC Bhashini Wijesinghe
    4. Duyoung Min
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This work presents an important methodological advance for single-molecule force spectroscopy of membrane proteins providing a new robust design of the linkage between a target single molecule and solid support. The data provide compelling evidence of the improved mechanical stability of the pulling system. Allowing more statistically reliable force measurements of biomolecules, this method may be broadly applicable in the field of single-molecule biophysics.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  3. Structural and mechanistic insights into the MCM8/9 helicase complex

    This article has 9 authors:
    1. Zhuangfeng Weng
    2. Jiefu Zheng
    3. Yiyi Zhou
    4. Zuer Lu
    5. Yixi Wu
    6. Dongyi Xu
    7. Huanhuan Li
    8. Huanhuan Liang
    9. Yingfang Liu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This paper presents important findings on the hexametric structure of MCM8/9, which potentially explain its role as a DNA helicase in homologous recombination. This solid work will be of interest to biologists studying DNA transactions.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 2 listsLatest version Latest activity
  4. Cannabidiol sensitizes TRPV2 channels to activation by 2-APB

    This article has 6 authors:
    1. Aaron Gochman
    2. Xiao-Feng Tan
    3. Chanhyung Bae
    4. Helen Chen
    5. Kenton J Swartz
    6. Andres Jara-Oseguera
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This is an important report on the discovery of a strong sensitizing effect of cannabidiol on the activation of TRPV2 channels by 2-APB. The conclusions are convincingly supported by solid electrophysiological recordings and cryo-EM structures, but identification of a clear molecular mechanism will require additional structural work. The paper will be of interest to the ion channel research community.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  5. Structure of the HIV immature lattice allows for essential lattice remodeling within budded virions

    This article has 4 authors:
    1. Sikao Guo
    2. Ipsita Saha
    3. Saveez Saffarian
    4. Margaret E Johnson
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This is a valuable study, carried out in a solid and comprehensive manner. The results advance the understanding of one of the steps of the HIV life cycle, via a better description of the mechanisms underlying Gag-Pol dimerization.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  6. The prolactin receptor scaffolds Janus kinase 2 via co-structure formation with phosphoinositide-4,5-bisphosphate

    This article has 9 authors:
    1. Raul Araya-Secchi
    2. Katrine Bugge
    3. Pernille Seiffert
    4. Amalie Petry
    5. Gitte W Haxholm
    6. Kresten Lindorff-Larsen
    7. Stine Falsig Pedersen
    8. Lise Arleth
    9. Birthe B Kragelund
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important interdisciplinary study substantially advances our understanding of the prolactin receptor interactions with the membrane lipids and the effect of these interactions on cell signaling. The authors use a combination of state-of-the-art NMR structural analysis, simulations, and cellular assays to provide compelling experimental evidence for protein complexes being regulated by IDR-membrane interactions. The work will be of broad interest to structural biologists and biochemists, and the results presented herein are likely relevant for other non-tyrosine kinase receptors.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  7. Force propagation between epithelial cells depends on active coupling and mechano-structural polarization

    This article has 13 authors:
    1. Artur Ruppel
    2. Dennis Wörthmüller
    3. Vladimir Misiak
    4. Manasi Kelkar
    5. Irène Wang
    6. Philippe Moreau
    7. Adrien Méry
    8. Jean Révilloud
    9. Guillaume Charras
    10. Giovanni Cappello
    11. Thomas Boudou
    12. Ulrich S Schwarz
    13. Martial Balland
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      Using surface micropatterning, optical activation, and theoretical analysis, the authors provide compelling evidence that adjacent cells actively propagate mechanical stress in epithelial tissues. The response of the receiver cell is active and enhanced when the principal stress direction is perpendicular to the orientation of actin fibers. This work is important and a must-read for everybody wanting to understand tissue mechanics.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  8. Regulation of chromatin microphase separation by binding of protein complexes

    This article has 5 authors:
    1. Omar Adame-Arana
    2. Gaurav Bajpai
    3. Dana Lorber
    4. Talila Volk
    5. Samuel Safran
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This fundamental work substantially advances our understanding of polymer physics underpinnings of genome folding, organization, and regulation. The conclusions are supported by both convincing computer simulations and analytical theory. The work will be of significant interest to the genome folding community.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  9. Structural and functional properties of the transporter SLC26A6 reveal mechanism of coupled anion exchange

    This article has 5 authors:
    1. David N Tippett
    2. Colum Breen
    3. Stephen J Butler
    4. Marta Sawicka
    5. Raimund Dutzler
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important manuscript combines cryo-EM and a suite of compelling whole cell and proteoliposome transport assays to establish the mechanism and structure of the full-length human SLC26A6 chloride/bicarbonate exchangers, including the first partial view of the previously unresolved IVS region of an SLC26 STAS domain. In combination with prior studies on additional SLC26 paralogs, including the SLC26A9 paralog initially reported by the same group, the study provides broadly relevant insights into the mechanistic diversity of the SLC26 transporters. This study is of interest to the biophysics community and the field of membrane transport.

    Reviewed by eLife

    This article has 9 evaluationsAppears in 1 listLatest version Latest activity
  10. Structural and functional properties of a plant NRAMP-related aluminum transporter

    This article has 8 authors:
    1. Karthik Ramanadane
    2. Márton Liziczai
    3. Dragana Markovic
    4. Monique S Straub
    5. Gian T Rosalen
    6. Anto Udovcic
    7. Raimund Dutzler
    8. Cristina Manatschal
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study provides valuable insight into the diversity within the NRAMP superfamily of transporters. Evidence of divalent metal ion transport and the structure (obtained without added metal ions) are convincing. However, molecular insight into Al3+ recognition and transport is incomplete, and the work would be strengthened by the determination of a metal-bound structure or additional experiments (such as molecular dynamics simulations or quantitative Al3+ binding/transport assays) to support the proposed Al3+ binding site. The work will be of interest to structural biologists and biophysicists studying NRAMP transporters.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
Previous Page 33 of 89 Next