Bioengineering

Heart Scar-In-A-Dish: Tissue Culture Platform to Study Myocardial Infarct Healing In Vitro

1. MJ Potter
2. JD Heywood
3. SJ Coeyman
4. WJ Richardson

Reviewed by preLights

Toward De Novo Protein Design from Natural Language

1. Fengyuan Dai
2. Shiyang You
3. Yudian Zhu
4. Yuan Gao
5. Lihao Fu
6. Xibin Zhou
7. Jin Su
8. Chentong Wang
9. Yuliang Fan
10. Xiaoxiao Ma
11. Xianjun Deng
12. Letong Yu
13. Hui Qian
14. Yan He
15. Yitao Ke
16. Chenchen Han
17. Xing Chang
18. Liangzhen Zheng
19. Sheng Wang
20. Yajie Wang
21. Anping Zeng
22. Shunzhi Wang
23. Tong Si
24. Jianming Liu
25. Hongyuan Lu
26. Fajie Yuan

Reviewed by Arcadia Science

Self-Organizing Neural Networks in Novel Moving Bodies: Anatomical, Behavioral, and Transcriptional Characterization of a Living Construct with a Nervous System

1. Haleh Fotowat
2. Laurie O’Neill
3. Léo Pio-Lopez
4. Megan Sperry
5. Patrick Erickson
6. Tiffany Lin
7. Michael Levin

Reviewed by Arcadia Science

Free‐Form Microfluidic Microneedle Array Patches

1. Ian A. Coates
2. Madison M. Driskill
3. Netra U. Rajesh
4. Gabriel Lipkowitz
5. Dan Ilyn
6. Yue Xu
7. Maria T. Dulay
8. Gunilla B. Jacobson
9. John R. Tumbleston
10. Jillian L. Perry
11. Shaomin Tian
12. Joseph M. DeSimone

Reviewed by Arcadia Science

Isolating Small Extracellular Vesicles from Small Volumes of Blood Plasma using size exclusion chromatography and density gradient ultracentrifugation: A Comparative Study

1. Fang Kong
2. Megha Upadya
3. Andrew See Wong Weng
4. Rinkoo Dalan
5. Ming Dao

• Curated by eLife

  eLife Assessment

  This work provides a simple, rapid and valuable protocol for the isolation of small extracellular vesicles from small volumes of plasma, using two well-known methodologies, in tandem: size exclusion chromatography (SEC) and density gradient ultracentrifugation (DGUC). The authors exhaustively test these methodologies separately and in combination, showing superior results for the SEC-DGUC in terms of purity and yield. The results obtained in this work are convincing, using multiple state-of-art methodologies for the characterization of the isolates that support their conclusions.

Reviewed by eLife

Development of antigen-dextramers for detection and evaluation of CAR T cells

1. Rasmus U. W. Friis
2. Maria Ormhøj
3. Cecilie S. Krüger-Jensen
4. Markus Barden
5. Keerthana Ramanathan
6. Mikkel R. Hansen
7. Hinrich Abken
8. Sine R. Hadrup

Reviewed by Arcadia Science

Lab-in-the-loop therapeutic antibody design with deep learning

1. Nathan C. Frey
2. Isidro Hötzel
3. Samuel D. Stanton
4. Ryan Kelly
5. Robert G. Alberstein
6. Emily K. Makowski
7. Karolis Martinkus
8. Daniel Berenberg
9. Jack Bevers
10. Tyler Bryson
11. Pamela Chan
12. Yongmei Chen
13. Alicja Czubaty
14. Tamica D’Souza
15. Henri Dwyer
16. Anna Dziewulska
17. James W. Fairman
18. Allen Goodman
19. Jennifer Hofmann
20. Henry Isaacson
21. Aya Ismail
22. Samantha James
23. Taylor Joren
24. Simon Kelow
25. James R. Kiefer
26. Matthieu Kirchmeyer
27. Joseph Kleinhenz
28. James T. Koerber
29. Julien Lafrance-Vanasse
30. Andrew Leaver-Fay
31. Jae Hyeon Lee
32. Edith Lee
33. Donald Lee
34. Wei-Ching Liang
35. Joshua Yao-Yu Lin
36. Sidney Lisanza
37. Andreas Loukas
38. Jan Ludwiczak
39. Sai Pooja Mahajan
40. Omar Mahmood
41. Homa Mohammadi-Peyhani
42. Santrupti Nerli
43. Ji Won Park
44. Jaewoo Park
45. Stephen Ra
46. Sarah Robinson
47. Saeed Saremi
48. Franziska Seeger
49. Imee Sinha
50. Anna M. Sokol
51. Natasa Tagasovska
52. Hao To
53. Edward Wagstaff
54. Amy Wang
55. Andrew M. Watkins
56. Blair Wilson
57. Shuang Wu
58. Karina Zadorozhny
59. John Marioni
60. Aviv Regev
61. Yan Wu
62. Kyunghyun Cho
63. Richard Bonneau
64. Vladimir Gligorijević

Reviewed by Arcadia Science

Mitochondrial adenine base editing of mouse somatic tissues via adeno-associated viral delivery

1. Christian D Mutti
2. Lindsey Van Haute
3. Lucia Luengo-Gutierrez
4. Keira Turner
5. Pedro Silva-Pinheiro
6. Michal Minczuk

• Curated by eLife

  eLife Assessment

  The authors have demonstrated the use of adenine base editors delivered via adeno-associated viruses to introduce edits in the mitochondrial genome. The manuscript describes the methodology well, and the conclusions are convincingly supported by the results. The valuable results highlight the potential of these base editors to model mtDNA variations in somatic tissues in animal models.

Reviewed by eLife

Scaly-tail organ enhances static stability during Pel’s scaly-tailed flying squirrels’ arboreal locomotion

1. Andrew K. Schulz
2. Mrudul Chellapurath
3. Pranav C. Khandelwal
4. SeyedReza Rezaei
5. Stefan Merker
6. Ardian Jusufi

Reviewed by preLights

Semantical and geometrical protein encoding toward enhanced bioactivity and thermostability

1. Yang Tan
2. Bingxin Zhou
3. Lirong Zheng
4. Guisheng Fan
5. Liang Hong

• Curated by eLife

  eLife Assessment

  ProtSSN is a valuable approach that generates protein embeddings by integrating sequence and structural information, demonstrating improved prediction of mutation effects on thermostability compared to competing models. The evidence supporting the authors' claims is compelling, with well-executed comparisons. This work will be of particular interest to researchers in bioinformatics and structural biology, especially those focused on protein function and stability.

Reviewed by eLife