Bioengineering

Magnetically steered cell therapy for reduction of intraocular pressure as a treatment strategy for open-angle glaucoma

1. M Reza Bahranifard
2. Jessica Chan
3. A Thomas Read
4. Guorong Li
5. Lin Cheng
6. Babak N Safa
7. Seyed Mohammad Siadat
8. Anamik Jhunjhunwala
9. Hans E Grossniklaus
10. Stanislav Y Emelianov
11. W Daniel Stamer
12. Markus H Kuehn
13. C Ross Ethier

• Curated by eLife

  eLife Assessment

  This study has fundamental findings that support the potential application of exogenous stem cell therapy as a viable therapeutic option for the management of intraocular pressure (IOP) and to increase outflow facility. The evidence supporting the clinical application of stem cells is compelling, using a combination of established in vivo and ex vivo experimental techniques. The work will be of interest to both basic stem cell biologists and clinical glaucoma specialists.

Reviewed by eLife

AI.zymes: A Modular Platform for Evolutionary Enzyme Design

1. Lucas P. Merlicek
2. Jannik Neumann
3. Abbie Lear
4. Vivian Degiorgi
5. Moor M. de Waal
6. Tudor‐Stefan Cotet
7. Adrian J. Mulholland
8. H. Adrian Bunzel

Reviewed by Arcadia Science

Antibody affinity engineering using antibody repertoire data and machine learning

1. Lena Erlach
2. Simon Friedensohn
3. Daniel Neumeier
4. Derek M. Mason
5. Sai T. Reddy

Reviewed by Arcadia Science

3D Mechanical Confinement Directs Muscle Stem Cell Fate and Function

1. GaYoung Park
2. Josh A. Grey
3. Foteini Mourkioti
4. Woojin M. Han

Reviewed by preLights

Heart Scar-In-A-Dish: Tissue Culture Platform to Study Myocardial Infarct Healing In Vitro

1. MJ Potter
2. JD Heywood
3. SJ Coeyman
4. WJ Richardson

Reviewed by preLights

Toward De Novo Protein Design from Natural Language

1. Fengyuan Dai
2. Shiyang You
3. Yudian Zhu
4. Yuan Gao
5. Lihao Fu
6. Xibin Zhou
7. Jin Su
8. Chentong Wang
9. Yuliang Fan
10. Xiaoxiao Ma
11. Xianjun Deng
12. Letong Yu
13. Hui Qian
14. Yan He
15. Yitao Ke
16. Chenchen Han
17. Xing Chang
18. Liangzhen Zheng
19. Sheng Wang
20. Yajie Wang
21. Anping Zeng
22. Shunzhi Wang
23. Tong Si
24. Jianming Liu
25. Hongyuan Lu
26. Fajie Yuan

Reviewed by Arcadia Science

Self-Organizing Neural Networks in Novel Moving Bodies: Anatomical, Behavioral, and Transcriptional Characterization of a Living Construct with a Nervous System

1. Haleh Fotowat
2. Laurie O’Neill
3. Léo Pio-Lopez
4. Megan Sperry
5. Patrick Erickson
6. Tiffany Lin
7. Michael Levin

Reviewed by Arcadia Science

Free‐Form Microfluidic Microneedle Array Patches

1. Ian A. Coates
2. Madison M. Driskill
3. Netra U. Rajesh
4. Gabriel Lipkowitz
5. Dan Ilyn
6. Yue Xu
7. Maria T. Dulay
8. Gunilla B. Jacobson
9. John R. Tumbleston
10. Jillian L. Perry
11. Shaomin Tian
12. Joseph M. DeSimone

Reviewed by Arcadia Science

Isolating Small Extracellular Vesicles from Small Volumes of Blood Plasma using size exclusion chromatography and density gradient ultracentrifugation: A Comparative Study

1. Fang Kong
2. Megha Upadya
3. Andrew See Wong Weng
4. Rinkoo Dalan
5. Ming Dao

• Curated by eLife

  eLife Assessment

  This work provides a simple, rapid and valuable protocol for the isolation of small extracellular vesicles from small volumes of plasma, using two well-known methodologies, in tandem: size exclusion chromatography (SEC) and density gradient ultracentrifugation (DGUC). The authors exhaustively test these methodologies separately and in combination, showing superior results for the SEC-DGUC in terms of purity and yield. The results obtained in this work are convincing, using multiple state-of-art methodologies for the characterization of the isolates that support their conclusions.

Reviewed by eLife

Development of antigen-dextramers for detection and evaluation of CAR T cells

1. Rasmus U. W. Friis
2. Maria Ormhøj
3. Cecilie S. Krüger-Jensen
4. Markus Barden
5. Keerthana Ramanathan
6. Mikkel R. Hansen
7. Hinrich Abken
8. Sine R. Hadrup

Reviewed by Arcadia Science