Bioengineering

Production of novel Spike truncations in Chinese hamster ovary cells

1. Shiaki A. Minami
2. Seongwon Jung
3. Yihan Huang
4. Bradley S. Harris
5. Matthew W. Kenaston
6. Roland Faller
7. Somen Nandi
8. Karen A. McDonald
9. Priya S. Shah

Reviewed by ScreenIT

Elucidating Design Principles for Engineering Cell‐Derived Vesicles to Inhibit SARS‐CoV‐2 Infection

1. Taylor F. Gunnels
2. Devin M. Stranford
3. Roxana E. Mitrut
4. Neha P. Kamat
5. Joshua N. Leonard

Reviewed by ScreenIT

Engineering RNA viruses with unnatural amino acid to evoke adjustable immune response in mice

1. Zhetao Zheng
2. Yu Wang
3. Xuesheng Wu
4. Haoran Zhang
5. Hongmin Chen
6. Haishuang Lin
7. Yuxuan Shen
8. Qing Xia

Reviewed by ScreenIT

Therapeutic downregulation of neuronal PAS domain 2 (Npas2) promotes surgical skin wound healing

1. Yoichiro Shibuya
2. Akishige Hokugo
3. Hiroko Okawa
4. Takeru Kondo
5. Daniel Khalil
6. Lixin Wang
7. Yvonne Roca
8. Adam Clements
9. Hodaka Sasaki
10. Ella Berry
11. Ichiro Nishimura
12. Reza Jarrahy

• Curated by eLife

  Evaluation Summary:

  The authors identify a novel compound called Dwn1 that suppresses the expression of Npas2, a key gene that delays wound healing. In doing so, they identify a novel treatment strategy for incisional surgical wounds that may have broader application to the treatment of scars in general.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

BSG/CD147 and ACE2 receptors facilitate SARS-CoV-2 infection of human iPS cell-derived kidney podocytes

1. Titilola D. Kalejaiye
2. Rohan Bhattacharya
3. Morgan A. Burt
4. Tatianna Travieso
5. Arinze E. Okafor
6. Xingrui Mou
7. Maria Blasi
8. Samira Musah

Reviewed by ScreenIT

Ultrapotent and broad neutralization of SARS-CoV-2 variants by modular, tetravalent, bi-paratopic antibodies

1. Shane Miersch
2. Nitin Sharma
3. Reza Saberianfar
4. Chao Chen
5. Francesca Caccuri
6. Alberto Zani
7. Arnaldo Caruso
8. James Brett Case
9. Michael S. Diamond
10. Gaya K. Amarasinghe
11. Giuseppe Novelli
12. Sachdev S. Sidhu

Reviewed by ScreenIT

Hemodynamic molecular imaging of tumor-associated enzyme activity in the living brain

1. Mitul Desai
2. Jitendra Sharma
3. Adrian L Slusarczyk
4. Ashley A Chapin
5. Robert Ohlendorf
6. Agata Wisniowska
7. Mriganka Sur
8. Alan Jasanoff

• Curated by eLife

  Evaluation Summary:

  This manuscript will be of interest to readers in the field of magnetic resonance imaging and responsive imaging probes. In this work, a new imaging probe is designed and applied in proof-of-principle animal models, with future promise for relevance in models that have higher relevance to human disease.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids

1. Jessica M. Vanslambrouck
2. Sean B. Wilson
3. Ker Sin Tan
4. Ella Groenewegen
5. Rajeev Rudraraju
6. Jessica Neil
7. Kynan T. Lawlor
8. Sophia Mah
9. Michelle Scurr
10. Sara E. Howden
11. Kanta Subbarao
12. Melissa H. Little

Reviewed by ScreenIT

Plant-based production of SARS-CoV-2 antigens for use in a subunit vaccine

1. Jordan Demone
2. Maryam Nourimand
3. Mariam Maltseva
4. Mina Nasr-Sharif
5. Yannick Galipeau
6. Marc-André Langlois
7. Allyson M. MacLean

Reviewed by ScreenIT

Temperature evolution following joint loading promotes chondrogenesis by synergistic cues via calcium signaling

1. Naser Nasrollahzadeh
2. Peyman Karami
3. Jian Wang
4. Lida Bagheri
5. Yanheng Guo
6. Philippe Abdel-Sayed
7. Lee Laurent-Applegate
8. Dominique P Pioletti

• Curated by eLife

  Evaluation Summary:

  This is the study to determine the effects of mechanical loading on temperature changes in the joint and how the mechano-thermal transduction may influence chondrocyte behavior. This manuscript will be of interest to the clinicians and researchers who are working on tissue engineering and cartilage regeneration. The study has high clinical relevance. It provides new evidence that the mechanical stimuli plus with the temperature increase could influence the cell chondrogenic response. The data support the conclusions of the manuscript within the current context, although several issues need to be addressed.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife