Bioengineering

Engineering RNA viruses with unnatural amino acid to evoke adjustable immune response in mice

1. Zhetao Zheng
2. Yu Wang
3. Xuesheng Wu
4. Haoran Zhang
5. Hongmin Chen
6. Haishuang Lin
7. Yuxuan Shen
8. Qing Xia

Reviewed by ScreenIT

Therapeutic downregulation of neuronal PAS domain 2 (Npas2) promotes surgical skin wound healing

1. Yoichiro Shibuya
2. Akishige Hokugo
3. Hiroko Okawa
4. Takeru Kondo
5. Daniel Khalil
6. Lixin Wang
7. Yvonne Roca
8. Adam Clements
9. Hodaka Sasaki
10. Ella Berry
11. Ichiro Nishimura
12. Reza Jarrahy

• Curated by eLife

  Evaluation Summary:

  The authors identify a novel compound called Dwn1 that suppresses the expression of Npas2, a key gene that delays wound healing. In doing so, they identify a novel treatment strategy for incisional surgical wounds that may have broader application to the treatment of scars in general.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

BSG/CD147 and ACE2 receptors facilitate SARS-CoV-2 infection of human iPS cell-derived kidney podocytes

1. Titilola D. Kalejaiye
2. Rohan Bhattacharya
3. Morgan A. Burt
4. Tatianna Travieso
5. Arinze E. Okafor
6. Xingrui Mou
7. Maria Blasi
8. Samira Musah

Reviewed by ScreenIT

Ultrapotent and broad neutralization of SARS-CoV-2 variants by modular, tetravalent, bi-paratopic antibodies

1. Shane Miersch
2. Nitin Sharma
3. Reza Saberianfar
4. Chao Chen
5. Francesca Caccuri
6. Alberto Zani
7. Arnaldo Caruso
8. James Brett Case
9. Michael S. Diamond
10. Gaya K. Amarasinghe
11. Giuseppe Novelli
12. Sachdev S. Sidhu

Reviewed by ScreenIT

Hemodynamic molecular imaging of tumor-associated enzyme activity in the living brain

1. Mitul Desai
2. Jitendra Sharma
3. Adrian L Slusarczyk
4. Ashley A Chapin
5. Robert Ohlendorf
6. Agata Wisniowska
7. Mriganka Sur
8. Alan Jasanoff

• Curated by eLife

  Evaluation Summary:

  This manuscript will be of interest to readers in the field of magnetic resonance imaging and responsive imaging probes. In this work, a new imaging probe is designed and applied in proof-of-principle animal models, with future promise for relevance in models that have higher relevance to human disease.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids

1. Jessica M. Vanslambrouck
2. Sean B. Wilson
3. Ker Sin Tan
4. Ella Groenewegen
5. Rajeev Rudraraju
6. Jessica Neil
7. Kynan T. Lawlor
8. Sophia Mah
9. Michelle Scurr
10. Sara E. Howden
11. Kanta Subbarao
12. Melissa H. Little

Reviewed by ScreenIT

Plant-based production of SARS-CoV-2 antigens for use in a subunit vaccine

1. Jordan Demone
2. Maryam Nourimand
3. Mariam Maltseva
4. Mina Nasr-Sharif
5. Yannick Galipeau
6. Marc-André Langlois
7. Allyson M. MacLean

Reviewed by ScreenIT

Temperature evolution following joint loading promotes chondrogenesis by synergistic cues via calcium signaling

1. Naser Nasrollahzadeh
2. Peyman Karami
3. Jian Wang
4. Lida Bagheri
5. Yanheng Guo
6. Philippe Abdel-Sayed
7. Lee Laurent-Applegate
8. Dominique P Pioletti

• Curated by eLife

  Evaluation Summary:

  This is the study to determine the effects of mechanical loading on temperature changes in the joint and how the mechano-thermal transduction may influence chondrocyte behavior. This manuscript will be of interest to the clinicians and researchers who are working on tissue engineering and cartilage regeneration. The study has high clinical relevance. It provides new evidence that the mechanical stimuli plus with the temperature increase could influence the cell chondrogenic response. The data support the conclusions of the manuscript within the current context, although several issues need to be addressed.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

SARS-CoV-2 causes human BBB injury and neuroinflammation indirectly in a linked organ chip platform

1. Peng Wang
2. Lin Jin
3. Min Zhang
4. Yunsong Wu
5. Zilei Duan
6. Wenwen Chen
7. Chaoming Wang
8. Zhiyi Liao
9. Jianbao Han
10. Yingqi Guo
11. Yaqiong Guo
12. Yaqing Wang
13. Ren Lai
14. Jianhua Qin

Reviewed by ScreenIT

Virtual mouse brain histology from multi-contrast MRI via deep learning

1. Zifei Liang
2. Choong H Lee
3. Tanzil M Arefin
4. Zijun Dong
5. Piotr Walczak
6. Song-Hai Shi
7. Florian Knoll
8. Yulin Ge
9. Leslie Ying
10. Jiangyang Zhang

• Curated by eLife

  Evaluation Summary:

  This paper demonstrates how MRI can be used to mimic histological measures. This is something that the field of MRI has dubbed virtual histology (or MR-histology) for a while, but to my knowledge this paper is the first convincing demonstration that it can be achieved. The paper combines open access mouse histology data from the Allen Institute with their own multimodal post-mortem MRI, and using deep convolutional networks, are able to build models that map MRI data onto multiple histological contrasts. Some of the results are impressive, such as predicting the outcome of histology on mutant shiverer mice.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife