Biochemistry

Fibroblast growth factor 21 regulates neuromuscular junction innervation through HDAC4 in denervation-induced skeletal muscle atrophy

1. Lirong Zheng
2. Takashi Sasaki
3. Liyang Ni
4. Yu Takahashi
5. Yoshio Yamauchi
6. Makoto Shimizu
7. Sato Ryuichiro

Reviewed by preLights

Development of D-box peptides to inhibit the Anaphase Promoting Complex/Cyclosome

1. Rohan Eapen
2. Cynthia Okoye
3. Christopher Stubbs
4. Marianne Schimpl
5. Thomas Tischer
6. Eileen J Fisher
7. Maria Zacharopoulou
8. Fernando Ferrer
9. David Barford
10. David Spring
11. Cath Lindon
12. Christopher Phillips
13. Laura S Itzhaki

• Curated by eLife

  eLife Assessment

  The manuscript represents a fundamental advance in designing peptide inhibitors targeting Cdc20, a key activator and substrate-recognition subunit of the APC/C ubiquitin ligase. Supported by compelling biophysical and cellular evidence, the study lays a strong foundation for future developments in degron-based therapeutics. The revised manuscript has been strengthened by additional clarifications and data that address prior reviewer concerns. The work provides a robust framework for developing tools to manipulate protein degradation and will be of broad interest to researchers in protein engineering, cell cycle regulation, and targeted protein degradation.

Reviewed by eLife

Identification of the Regulatory Elements and Protein Substrates of Lysine Acetoacetylation

1. Qianyun Fu
2. Terry Nguyen
3. Bhoj Kumar
4. Parastoo Azadi
5. Y George Zheng

• Curated by eLife

  eLife Assessment

  This useful study reports a method to detect and analyze a novel post-translational modification, lysine acetoacetylation (Kacac), finding it regulates protein metabolism pathways. The study unveils epigenetic modifiers involved in placing this mark, including key histone acetyltransferases such as p300, and concomitant HDACs, which remove the mark. Proteomic and bioinformatics analysis identified many human proteins with Kacac sites, potentially suggesting broad effects on cellular processes and disease mechanisms. While the data presented are solid, the functional validation of the sites would add significantly to the manuscript's description of this modification; the study will be of interest to those studying protein and metabolic regulation.

Reviewed by eLife

Solubilization of Membrane Proteins using designed protein WRAPS

1. Ljubica Mihaljević
2. David E. Kim
3. Helen E. Eisenach
4. Pooja D. Bandawane
5. Andrew J. Borst
6. Alexis Courbet
7. Everton Bettin
8. Qiushi Liu
9. Connor Weidle
10. Sagardip Majumder
11. Xinting Li
12. Mila Lamb
13. Analisa Nicole Azcárraga Murray
14. Rashmi Ravichandran
15. Elizabeth C. Williams
16. Shuyuan Hu
17. Lynda Stuart
18. Linda Grillová
19. Nicholas R. Thomson
20. Pengxiang Chang
21. Melissa J. Caimano
22. Kelly L. Hawley
23. Neil P. King
24. David Baker

Reviewed by Rapid Reviews Infectious Diseases

Force transmission through the inner kinetochore is enhanced by centromeric DNA sequences

1. Elise Miedlar
2. Grace E Hamilton
3. Samuel R Witus
4. Sara J Gonske
5. Michael Riffle
6. Alex Zelter
7. Rachel E Klevit
8. Charles L Asbury
9. Yoana N Dimitrova
10. Trisha N Davis

• Curated by eLife

  eLife Assessment

  Centromeres are specific sites on chromosomes that are essential for mitosis and genome fidelity. This valuable research advance builds upon previous studies to convincingly show that the centromere-histone core contributes to force transduction through the kinetochore. The centromere mainly strengthens one of the two paths of force transduction, influenced by the centromeric DNA sequence. The mechanism underlying this phenomenon will be an exciting future avenue of research, given that centromeric DNAs are not conserved. This work will be of interest to those studying cell division and chromosome segregation.

Reviewed by eLife

Trifluoroacetate reduces plasma lipid levels and the development of atherosclerosis in mice

1. Wei Tang
2. Audrey S. Black
3. Romana Moench
4. Katayoon Marzban
5. Juan Antonio Raygoza Garay
6. James J. Zheng
7. Louis Conway
8. Antonio F. M. Pinto
9. Christopher G. Parker
10. Alan Saghatelian
11. Luke J. Leman
12. M. Reza Ghadiri

Reviewed by PREreview

Heterooligomerization drives structural plasticity of eukaryotic peroxiredoxins

1. Jannik Zimmermann
2. Lukas Lang
3. Julia Malo Pueyo
4. Mareike Riedel
5. Khadija Wahni
6. Dylan Stobbe
7. Christopher Lux
8. Steven Janvier
9. Didier Vertommen
10. Svenja Lenhard
11. Frank Hannemann
12. Helena Castro
13. Ana Maria Tomas
14. Johannes M. Herrmann
15. Armindo Salvador
16. Timo Mühlhaus
17. Jan Riemer
18. Joris Messens
19. Marcel Deponte
20. Bruce Morgan

Reviewed by PREreview

Mycobacterial EsxG·EsxH (TB9.8·TB10.4) peptides as a subunit vaccine to booster BCG vaccination in an experimental model of pulmonary Tuberculosis

1. Constanza Estefania Martínez-Olivares
2. Vasti Lozano-Ordaz
3. Dulce Mata-Espinosa
4. Jorge Alberto Barrios-Payán
5. Ángel Elías Ortiz-Cabrera
6. Yadira Rocio Rodríguez-Miguez
7. Rogelio Hernández-Pando

Reviewed by PREreview

Stabilization of GTSE1 by cyclin D1–CDK4/6-mediated phosphorylation promotes cell proliferation with implications for cancer prognosis

1. Nelson García-Vázquez
2. Tania J González-Robles
3. Ethan Lane
4. Daria Spasskaya
5. Qingyue Zhang
6. Marc A Kerzhnerman
7. YeonTae Jeong
8. Marta Collu
9. Daniele Simoneschi
10. Kelly V Ruggles
11. Gergely Róna
12. Sharon Kaisari
13. Michele Pagano

• Curated by eLife

  eLife Assessment

  In this article, García-Vázquez et al. report valuable findings demonstrating that G2 and S phases expressed protein 1 (GTSE1), is a previously unappreciated non-pocket substrate of the cyclin D/cyclin-dependent kinase (CDK) 4/6 axis. The authors provide convincing evidence showing that this mechanism is triggered in pathological states in which cyclin D levels are elevated (e.g., cancer). Overall, this study holds a promise to improve understanding of the mechanisms underpinning cell cycle progression including its dysregulation in neoplasia and may thus be of broad interest to researchers belonging to diverse biomedical disciplines ranging from cancer research to cell biology.

Reviewed by eLife

Engineering cardiolipin binding to an artificial membrane protein reveals determinants for lipid-mediated stabilization

1. Mia L Abramsson
2. Robin A Corey
3. Jan L Skerle
4. Louise J Persson
5. Olivia Anden
6. Abraham O Oluwole
7. Rebecca J Howard
8. Erik Lindahl
9. Carol V Robinson
10. Kvido Strisovsky
11. Erik G Marklund
12. David Drew
13. Phillip J Stansfeld
14. Michael Landreh

• Curated by eLife

  eLife Assessment

  Cardiolipin is known to play an important role in modulating the assembly and function of membrane proteins in bacterial and mitochondrial membranes. Here, authors convincingly define the molecular determinants of cardiolipin binding on de novo-designed and native membrane proteins combining the coarse-grained molecular dynamics simulation with the state-of-the-art experimental approaches such as native mass spectrometry and cryogenic electron microscopy. The major findings in this study, which are the identification of degenerate cardiolipin binding motifs, the characterization of their dynamic features, and the role in membrane protein stability and activity, will provide much needed insight into the still poorly understood nature of protein-cardiolipin interactions.

Reviewed by eLife