Biochemistry

Systematic investigation of the link between enzyme catalysis and cold adaptation

1. Catherine Stark
2. Teanna Bautista-Leung
3. Joanna Siegfried
4. Daniel Herschlag

• Curated by eLife

  Evaluation Summary:

  Are enzymes found in organisms that optimally grow at colder temperatures are more active than the same enzymes found in organisms that optimally grow at warmer temperatures? Here, an assessment of the catalytic constants for approximately 2200 enzymes (obtained from the BRENDA database) showed no correlation between the relative catalytic activity and the optimum growth temperature. Further support for this conclusion was obtained from the measurement of the catalytic constant from a selection of ketosteroid isomerases from organisms that optimally grow between 15 and 46 degrees centigrade. These are interesting results, although the significance with respect to earlier studies has not been clearly explained.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Regulation of ERK2 activity by dynamic S-acylation

1. Saara-Anne Azizi
2. Tian Qiu
3. Noah E. Brookes
4. Bryan C. Dickinson

• Curated by eLife

  Evaluation Summary:

  This manuscript describes the regulation of ERK1/2, two protein kinases that play important roles in cell signaling, by protein cysteine palmitoylation. The intriguing observations reported here could be of broad interest to colleagues in the field of cell signaling and protein post-translational modifications. Mechanistic understanding is, however, still limited and the work would benefit from additional experimental evidence.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Deep Mutational Engineering of broadly-neutralizing and picomolar affinity nanobodies to accommodate SARS-CoV-1 & 2 antigenic polymorphism

1. Adrien Laroche
2. Maria Lucia Orsini Delgado
3. Philippe Cuniasse
4. Steven Dubois
5. Raphaël Sierocki
6. Fabrice Gallais
7. Stéphanie Debroas
8. Laurent Bellanger
9. Stéphanie Simon
10. Bernard Maillère
11. Hervé Nozach

Reviewed by ScreenIT

Mutations in the spike RBD of SARS-CoV-2 omicron variant may increase infectivity without dramatically altering the efficacy of current multi-dosage vaccinations

1. Bingrui Li
2. Xin Lu
3. Kathleen M. McAndrews
4. Raghu Kalluri

Reviewed by ScreenIT

Cell-surface tethered promiscuous biotinylators enable comparative small-scale surface proteomic analysis of human extracellular vesicles and cells

1. Lisa L Kirkemo
2. Susanna K Elledge
3. Jiuling Yang
4. James R Byrnes
5. Jeff E Glasgow
6. Robert Blelloch
7. James A Wells

• Curated by eLife

  Evaluation Summary:

  This report describes a new technique to detect the surface proteome of normal and myc-transformed cells in relation to extracellular vesicles from the same cells. The data obtained from this comparison may be useful in evaluating cell surface and extracellular vesicle marker proteins that may be of diagnostic value. The article could possibly be more interesting if the actual proteomic results of control vs Myc and cells vs extracellular vesicles were more extensively exploited.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

A proteomic perspective and involvement of cytokines in SARS-CoV-2 infection

1. Sarena Banu
2. Ramakrishnan Nagaraj
3. Mohammed M. Idris

Reviewed by ScreenIT

The High Transmission of SARS-CoV-2 Omicron (B.1.1.529) Variant is Not Only Due to Its hACE2 binding: A Free Energy of Perturbation Study

1. Filip Fratev

Reviewed by ScreenIT

Interactions of SARS-CoV-2 protein E with cell junctions and polarity PDZ-containing proteins

1. Yanlei Zhu
2. Flavio Alvarez
3. Nicolas Wolff
4. Ariel Mechaly
5. Sébastien Brûlé
6. Benoit Neitthoffer
7. Sandrine Etienne-Manneville
8. Ahmed Haouz
9. Batiste Boëda
10. Célia Caillet-Saguy

Reviewed by ScreenIT

Alzheimer's Aβ assembly binds sodium pump and blocks endothelial NOS activity via ROS-PKC pathway in brain vascular endothelial cells

1. Tomoya Sasahara
2. Kaori Satomura
3. Mari Tada
4. Akiyoshi Kakita
5. Minako Hoshi

• Curated by eLife

  Evaluation Summary:

  This paper examines the effects of amylospheroids, highly neurotoxic assemblies of β-amyloid, on aortic function and on cultured cells. The authors propose that the interaction of amylospheroids with the sodium pump in endothelial cells induces production of reactive oxygen species to ultimately comprise nitric oxide generation. The study provides some new insight into mechanisms underlying brain blood vessel dysfunction and will be interesting neuroscientists who study neurovascular contribution to neurodegenerative diseases. The conclusions of the manuscript are supported by the data, but alternative approaches would make the study stronger.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Possible Interference in Protein – Protein interaction as a new approach in microinhibition of respiratory pathogens on nasal– oral epithelium: An early on-screen study with reference toSARS-Cov-2–ACE2 binding interference

1. Debatosh Datta
2. Suyash Pant
3. Devendra Kumar Dhaked
4. Somasundaram Arumugam
5. Ravichandiran Velayutham
6. Pallab Datta

Reviewed by ScreenIT