Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
-
SciScore for 10.1101/2022.04.07.487528: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Antibodies were incubated at room temperature for 1 hour, washed and binding detected with goat anti-human-HRP (Jackson ImmunoResearch Laboratories, PA) and TMB substrate. anti-human-HRPsuggested: NoneMouse anti-monkey IgG-HRP (Southern Biotech, CAT# 4700-05) and Goat anti-human IgG-HRP (Jackson ImmunoResearch Laboratories, CAT# 109-035-098) secondary antibodies were used to detect antibody bound to the spike proteins. anti-monkey IgG-HRPsuggested: (SouthernBiotech Cat# 4700-05, RRID:AB_…SciScore for 10.1101/2022.04.07.487528: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Antibodies were incubated at room temperature for 1 hour, washed and binding detected with goat anti-human-HRP (Jackson ImmunoResearch Laboratories, PA) and TMB substrate. anti-human-HRPsuggested: NoneMouse anti-monkey IgG-HRP (Southern Biotech, CAT# 4700-05) and Goat anti-human IgG-HRP (Jackson ImmunoResearch Laboratories, CAT# 109-035-098) secondary antibodies were used to detect antibody bound to the spike proteins. anti-monkey IgG-HRPsuggested: (SouthernBiotech Cat# 4700-05, RRID:AB_2796069)anti-human IgG-HRPsuggested: NoneRBD binding to IgG’s was assessed using a Series S CM5 chip (Cytiva, MA) which was labeled with anti-human IgG (fc) antibody using a Human Antibody Capture Kit (Cytiva, MA). anti-human IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Antibodies were produced in Expi293 cells (embryonal, human kidney). Expi293suggested: RRID:CVCL_D615)293T/ACE2-MF cells were detached from T75 culture flasks using TrypLE Select Enzyme solution, suspended in growth medium (100,000 cells/ml) and immediately added to all wells (10,000 cells in 100 µL of growth medium per well). 293T/ACE2-MFsuggested: NoneRecombinant DNA Sentences Resources The sequence was codon optimized for mammalian expression and subcloned into the pCAGGS mammalian expression vector under the AG promoter. pCAGGSsuggested: RRID:Addgene_127347)Software and Algorithms Sentences Resources https://www.addgene.org). https://www.addgene.orgsuggested: (Addgene, RRID:SCR_002037)Sensogram data were analyzed using the BiaEvaluation software (Cytiva, MA) BiaEvaluationsuggested: (BIAevaluation Software, RRID:SCR_015936)The cryoSPARC (Punjani et al., 2017) software was used for data processing. cryoSPARCsuggested: (cryoSPARC, RRID:SCR_016501)Coot (Emsley et al., 2010) Cootsuggested: (Coot, RRID:SCR_014222), Pymol (Schrodinger, 2015), Chimera (Pettersen et al., 2004), ChimeraX (Goddard et al., 2018) and Isolde (Croll, 2018) were used for model building and refinement. Pymolsuggested: (PyMOL, RRID:SCR_000305)Difference distance matrices (DDM): DDM were generated using the Bio3D package (Grant et al., 2020) implemented in R (R Core Team (2014). Bio3Dsuggested: NoneResults from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
-