Biochemistry

Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission

1. Dawn M Wenzel
2. Douglas R Mackay
3. Jack J Skalicky
4. Elliott L Paine
5. Matthew S Miller
6. Katharine S Ullman
7. Wesley I Sundquist

• Curated by eLife

  Evaluation Summary:

  The authors quantitatively characterize binding interactions between all known MIM motifs of ESCRT-III proteins with the MIT motifs of several AAA+ ATPases. In addition to the analysis of these interactions, which will be an important resource to the ESCRT community, the authors also identify new roles of the ATPases SPASTIN, KATNA1 and CAPN7 in cytokinesis. Therefore, the work will be of broad interest to biologists interested in membrane-associated complexes and in cell cycle and cytokinesis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Dynamics of allosteric regulation of the phospholipase C-γ isozymes upon recruitment to membranes

1. Edhriz Siraliev-Perez
2. Jordan TB Stariha
3. Reece M Hoffmann
4. Brenda RS Temple
5. Qisheng Zhang
6. Nicole Hajicek
7. Meredith L Jenkins
8. John E Burke
9. John Sondek

• Curated by eLife

  Evaluation Summary:

  This work provides insight into how phospholipase C gamma (PLCγ1) becomes activated upon binding to phosphorylated receptor tyrosine kinase, with an analysis of PLC γ1 bound to the soluble kinase domain of FGFR1 (FGFR1K) and/or liposomes containing PIP2. The most interesting finding is that regions of the protein far from the FGFR1K binding site increase in exchange upon binding. This is new information for a large protein that is arguably difficult to study, but it conforms to what has been observed in many other autoinhibited systems with similar SH2 and SH3 domains such as kinases. The results will be of interest to structural biologists and cell biologists with interest in the mechanisms leading to the regulation of phospholipase C activity on membranes.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Hypertrophic cardiomyopathy mutations in the pliant and light chain-binding regions of the lever arm of human β-cardiac myosin have divergent effects on myosin function

1. Makenna M Morck
2. Debanjan Bhowmik
3. Divya Pathak
4. Aminah Dawood
5. James Spudich
6. Kathleen M Ruppel

• Curated by eLife

  Evaluation Summary:

  This work is of broad interest to readers in the fields of cytoskeletal research, muscle biology and heart disease. By utilizing a combination of quantitative biochemical and biophysical experimental approaches, this work provides critical new insights into the molecular mechanisms of understudied mutations in myosin that cause heart disease. The data are rigorously controlled and analyzed and support the claims of the work.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Structural basis for RNA-duplex unwinding by the DEAD-box helicase DbpA

1. Jan Philip Wurm

• Curated by eLife

  Evaluation Summary:

  This manuscript is interesting to a broad audience in the general fields of RNA and structural biology. It provides detailed and important molecular insight into one of the mechanisms by which ATP-fueled RNA helicases can cause the local destabilisation of terminal base-pairs and eventually contribute to RNA structure remodelling and it is a prime example of how crystallographic high-resolution snapshots of conformational intermediates can be combined with sophisticated NMR techniques and assays into a comprehensive model. The manuscript would benefit from a broader and more explicit comparative discussion including the limitations of the proposed model, because DbpA is a rather specialised RNA helicase and because the double-stranded RNA substrates were specifically designed to exclusively investigate unwinding from the side of a short 5'-overhang.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.

Reviewed by eLife

Antibacterial potency of type VI amidase effector toxins is dependent on substrate topology and cellular context

1. Atanas Radkov
2. Anne L Sapiro
3. Sebastian Flores
4. Corey Henderson
5. Hayden Saunders
6. Rachel Kim
7. Steven Massa
8. Samuel Thompson
9. Chase Mateusiak
10. Jacob Biboy
11. Ziyi Zhao
12. Lea M Starita
13. William L Hatleberg
14. Waldemar Vollmer
15. Alistair B Russell
16. Jean-Pierre Simorre
17. Spencer Anthony-Cahill
18. Peter Brzovic
19. Beth Hayes
20. Seemay Chou

Reviewed by Review Commons

SARS-CoV-2 spike variants differ in their allosteric response to linoleic acid

1. A. Sofia F. Oliveira
2. Deborah K. Shoemark
3. Andrew D. Davidson
4. Imre Berger
5. Christiane Schaffitzel
6. Adrian J. Mulholland

Reviewed by ScreenIT

The free fatty acid–binding pocket is a conserved hallmark in pathogenic β-coronavirus spike proteins from SARS-CoV to Omicron

1. Christine Toelzer
2. Kapil Gupta
3. Sathish K. N. Yadav
4. Lorna Hodgson
5. Maia Kavanagh Williamson
6. Dora Buzas
7. Ufuk Borucu
8. Kyle Powers
9. Richard Stenner
10. Kate Vasileiou
11. Frederic Garzoni
12. Daniel Fitzgerald
13. Christine Payré
14. Gunjan Gautam
15. Gérard Lambeau
16. Andrew D. Davidson
17. Paul Verkade
18. Martin Frank
19. Imre Berger
20. Christiane Schaffitzel

Reviewed by ScreenIT

Target binding triggers hierarchical phosphorylation of human Argonaute-2 to promote target release

1. Brianna Bibel
2. Elad Elkayam
3. Steve Silletti
4. Elizabeth A Komives
5. Leemor Joshua-Tor

• Curated by eLife

  Evaluation Summary:

  This paper provides well documented and solid biochemical data to show how phosphorylation of AGO2 modulate its binding to target mRNAs, releasing the complex to allow its recycling in the cell via electrostatic repulsion. This result could explain how a small amount of Ago proteins could target a very large number of mRNA molecules . The data support the key claims of the manuscript, and the approaches used are rigorous. This very well-written and elegant study will be of great interest to those working in the miRNA field as it addresses important open questions concerning the dynamic regulation of mIRNA-mediated gene repression.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Redox regulation of the SARS-CoV-2 main protease provides new opportunities for drug design

1. Lisa-Marie Funk
2. Gereon Poschmann
3. Ashwin Chari
4. Fabian Rabe von Pappenheim
5. Kim-Maren Stegmann
6. Antje Dickmanns
7. Nora Eulig
8. Marie Wensien
9. Elham Paknia
10. Gabi Heyne
11. Elke Penka
12. Arwen R. Pearson
13. Carsten Berndt
14. Tobias Fritz
15. Sophia Bazzi
16. Jon Uranga
17. Ricardo A. Mata
18. Matthias Dobbelstein
19. Rolf Hilgenfeld
20. Ute Curth
21. Kai Tittmann

Reviewed by ScreenIT

Uncovering the structural flexibility of SARS-CoV-2 glycoprotein spike variants

1. Hiam R. S. Arruda
2. Tulio M. Lima
3. Renata G. F. Alvim
4. Fernanda B. A. Victorio
5. Daniel P. B. Abreu
6. Federico F. Marsili
7. Karen D. Cruz
8. Patricia Sosa-Acosta
9. Mauricio Quinones-Vega
10. Jéssica de S. Guedes
11. Fábio C. S. Nogueira
12. Jerson L. Silva
13. Leda R. Castilho
14. Guilherme A. P. de Oliveira

Reviewed by ScreenIT