1. Rac1 Selectively Binds a Specific Lamellipodin Isoform via a Noncanonical Helical Interface

    This article has 5 authors:
    1. Tong Gao
    2. Pingfeng Zhang
    3. Alison M. Kurimchak
    4. James S. Duncan
    5. Jinhua Wu

    Reviewed by Arcadia Science

    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
  2. Erythrocytosis-inducing PHD2 mutations implicate biological role for N-terminal prolyl-hydroxylation in HIF1α oxygen-dependent degradation domain

    This article has 8 authors:
    1. Cassandra C Taber
    2. Wenguang He
    3. Geneviève MC Gasmi-Seabrook
    4. Mia Hubert
    5. Fraser G Ferens
    6. Mitsuhiko Ikura
    7. Jeffery E Lee
    8. Michael Ohh
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      In this valuable study, Taber et al used a battery of biophysical and structural approaches to characterize the impact of erythrocytosis-related mutations in prolyl hydroxylase domain protein 2 (PHD2). The authors show that PHD2 mutant proteins are destabilized, thus supporting the tenet that dysregulation of PHD2/hypoxia induced factor (HIF) axis underpins erythrocytosis, while providing incomplete evidence that N-terminal ODD prolyl hydroxylation of HIF is indispensable for these phenotypes. Notwithstanding that this study was found to be of broad interest for a variety of fields focusing on oxygen sensing in homeostasis and pathological states, resolving inconsistencies in the biophysical analysis (e.g., NMR, SEC, and BLI/MST) was thought to be warranted to further corroborate the proposed model.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  3. Engineering NIR-Sighted Bacteria

    This article has 7 authors:
    1. Stefanie SM Meier
    2. Michael Hörzing
    3. Cornelia Böhm
    4. Emma LR Düthorn
    5. Heikki Takala
    6. René Uebe
    7. Andreas Möglich
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study establishes bathy phytochromes, a unique class of bacterial photoreceptors that respond to near-infrared light (NIR), as important tools for bacterial optogenetics. NIR light is a key control signal in optogenetics due to its deep tissue penetration and the ability to combine with existing red- and blue-light sensitive systems, but thus far, NIR-activated proteins have been poorly characterized. The strength of the evidence is solid overall, with comprehensive in vitro characterization, modular design strategies, and validation across different hosts. There are some questions that remain such as the rationale for linker choices, characterization of growth and performance relative to controls, and the physiological significance of color blind effects at alkaline pH but overall, this study should advance the fields of optogenetics and photobiology and inspire future work.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  4. The temperature dependence of binding entropy is a selective pressure in protein evolution

    This article has 8 authors:
    1. Rosemary Georgelin
    2. Hannah Bott
    3. Joe A. Kaczmarski
    4. Rebecca Frkic
    5. Li Lynn Tan
    6. Nobuhiko Tokuriki
    7. Matthew A. Spence
    8. Colin J. Jackson

    Reviewed by PREreview

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
  5. Categorizing prediction modes within low-pLDDT regions of AlphaFold2 structures

    This article has 4 authors:
    1. Christopher J Williams
    2. Vincent B Chen
    3. David C Richardson
    4. Jane S Richardson

    Reviewed by Arcadia Science

    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
  6. Phage-displayed synthetic library and screening platform for nanobody discovery

    This article has 10 authors:
    1. Baolong Xia
    2. Ah-Ram Kim
    3. Feimei Liu
    4. Myeonghoon Han
    5. Emily Stoneburner
    6. Stephanie Makdissi
    7. Francesca Di Cara
    8. Stephanie E Mohr
    9. Aaron M Ring
    10. Norbert Perrimon
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study presents an alternative platform for nanobody discovery using phage-displayed synthetic libraries. The evidence supporting the platform, which is used to isolate and validate nanobodies targeting Drosophila secreted proteins, is compelling. By making the library openly accessible, this provides an excellent resource to the wider scientific community. The paper presents a detailed protocol for nanobody screening; as this protocol is refined and optimized over time, this will increase the success rate for discovering nanobodies with improved properties using this alternative platform.

    Reviewed by eLife

    This article has 6 evaluationsAppears in 1 listLatest version Latest activity
  7. Cryo-EM structure revealed a novel F-actin binding motif in a Legionella pneumophila lysine fatty-acyltransferase

    This article has 5 authors:
    1. Wenjie W Zeng
    2. Garrison Komaniecki
    3. Jiaze Liu
    4. Hening Lin
    5. Yuxin Mao
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study identifies a novel Legionella effector, Lfat1, which binds F-actin via a coiled-coil domain and structurally resembles the RID toxin, with cryo-EM revealing key interactions mediated by a hydrophobic helical hairpin. While the study is mostly complete and has compelling data, a few minor changes are recommended.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  8. Cardiolipin deficiency disrupts electron transport chain to drive steatohepatitis

    This article has 35 authors:
    1. Marisa J Brothwell
    2. Guoshen Cao
    3. J Alan Maschek
    4. Annelise M Poss
    5. Alek D Peterlin
    6. Liping Wang
    7. Talia B Baker
    8. Justin L Shahtout
    9. Piyarat Siripoksup
    10. Quentinn J Pearce
    11. Jordan M Johnson
    12. Fabian M Finger
    13. Alexandre Prola
    14. Sarah A Pellizzari
    15. Gillian L Hale
    16. Allison M Manuel
    17. Shinya Watanabe
    18. Edwin R Miranda
    19. Kajsa E Affolter
    20. Trevor S Tippetts
    21. Linda S Nikolova
    22. Ran Hee Choi
    23. Stephen T Decker
    24. Mallikarjun Patil
    25. J Leon Catrow
    26. William L Holland
    27. Sara M Nowinski
    28. Daniel S Lark
    29. Kelsey H Fisher-Wellman
    30. Patrice N Mimche
    31. Kimberley J Evason
    32. James E Cox
    33. Scott A Summers
    34. Zach Gerhart-Hines
    35. Katsuhiko Funai
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This paper reports on a correlation between diminished cardiolipin content and the severity of steatohepatitis in human subjects. This is supported further by experimental evidence from mice in which the gene encoding a key enzyme in cardiolipin synthesis has been compromised in the liver. The correlations established between lipidology, mitochondrial function, and the induction of respiration and oxidative stress are notable and will be useful to researchers in the field. However, given that the causal relationship between lipid perturbation and the progression of steatohepatitis implied in the title has not been tested experimentally, the evidence supporting the paper's key conclusion is incomplete.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  9. Elevated Ubiquitin Phosphorylation by PINK1 Contributes to Proteasomal Impairment and Promotes Neurodegeneration

    This article has 11 authors:
    1. Cong Chen
    2. Tong-Yao Gao
    3. Hua-Wei Yi
    4. Yi Zhang
    5. Tong Wang
    6. Zhi-Lin Lou
    7. Tao-Feng Wei
    8. Yun-Bi Lu
    9. Ting-Ting Li
    10. Chun Tang
    11. Wei-Ping Zhang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study provides important insights into the role of polyUbiquitination in neurodegenerative diseases, elucidating how pUb promotes neurodegeneration by affecting proteasomal function. The findings not only offer a new perspective on the pathophysiology of neurodegenerative diseases but also provide potential targets for developing new therapeutic strategies. The results provide solid evidence to support the conclusions.

    Reviewed by eLife

    This article has 11 evaluationsAppears in 1 listLatest version Latest activity
  10. The Structural Dynamics of IRE1 and its Interaction with Unfolded Peptides

    This article has 3 authors:
    1. Elena Spinetti
    2. G Elif Karagöz
    3. Roberto Covino
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      In this important study, the authors conducted atomistic molecular dynamics simulations to probe the interactions between IRE and unfolded peptides. The results help reconcile contradicting experimental findings in the literature and offer mechanistic insights into the activation of the unfolded protein response. The level of evidence is considered solid, although the use of enhanced sampling and more quantitative analysis would further strengthen the conclusions.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
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