Biochemistry

Specific proteolysis mediated by a p97-directed proteolysis-targeting chimera (PROTAC)

1. Constanza Salinas-Rebolledo
2. Javier Blesa
3. Guillermo Valenzuela-Nieto
4. David Schwefel
5. Natalia López González del Rey
6. Maxs Méndez-Ruette
7. Janine Burkhalter
8. Luis Federico Bátiz
9. Ronald Jara
10. José A. Obeso
11. Pedro Chana-Cuevas
12. Gopal P. Sapkota
13. Alejandro Rojas-Fernandez

Reviewed by Review Commons

Plasticity of the proteasome-targeting signal Fat10 enhances substrate degradation

1. Hitendra Negi
2. Aravind Ravichandran
3. Pritha Dasgupta
4. Shridivya Reddy
5. Ranabir Das

• Curated by eLife

  eLife assessment

  This manuscript probes the ways in which a protein tag might influence the structure, dynamics and stability of a covalently-attached substrate protein. Such findings are of important significance to several fields, particularly in understanding how these influences control the abundance of proteins within a cell. The evidence provided to support the authors' conclusions are, however, incomplete and further control experiments are necessary to fully support the proposed model.

Reviewed by eLife

Senolytics enhance longevity in Caenorhabditis elegans by altering betaine metabolism

1. Wenning Lan
2. Xiaolian Xiao
3. Xiaojing Zhang
4. Jingjing Nian
5. Ziran Wang
6. Yajiao Wu
7. Dongcheng Zhang
8. Junkun Chen
9. Wenqiang Bao
10. Chutao Li
11. An Zhu
12. Yun Zhang
13. Fangrong Zhang

Reviewed by PREreview

Human DDX6 regulates translation and decay of inefficiently translated mRNAs

1. Ramona Weber
2. Chung-Te Chang

• Curated by eLife

  eLife assessment

  This study provides valuable findings that improve our understanding of the evolutionary conservation of the role of DDX6 in mRNA decay. The evidence supporting the authors' conclusions is convincing. This work will be of interest to molecular, cell biologists and biochemists, especially those studying RNA.

Reviewed by eLife

idh-1 neomorphic mutation confers sensitivity to vitamin B12 via increased dependency on one-carbon metabolism in Caenorhabditis elegans

1. Olga Ponomarova
2. Alyxandra N. Starbard
3. Alexandra Belfi
4. Amanda V. Anderson
5. Meera V. Sundaram
6. Albertha J.M. Walhout

Reviewed by Review Commons

PARP1 condensates differentially partition DNA repair proteins and enhance DNA ligation

1. Christopher Chin Sang
2. Gaelen Moore
3. Maria Tereshchenko
4. Michael L. Nosella
5. Hongshan Zhang
6. T. Reid Alderson
7. Morgan Dasovich
8. Anthony Leung
9. Ilya J. Finkelstein
10. Julie D. Forman-Kay
11. Hyun O. Lee

Reviewed by Review Commons

Senescent cells inhibit mouse myoblast differentiation via the SASP-lipid 15d-PGJ2 mediated modification and control of HRas

1. Swarang Sachin Pundlik
2. Alok Barik
3. Ashwin Venkateshvaran
4. Snehasudha Subhadarshini Sahoo
5. Mahapatra Anshuman Jaysingh
6. Raviswamy GH Math
7. Heera Lal
8. Maroof Athar Hashmi
9. Arvind Ramanathan

• Curated by eLife

  eLife assessment

  This manuscript outlines an interaction between senescence-related 15d-PGJ2 and the proliferation and differentiation of myoblasts, with potential implications for muscle health. This manuscript is useful in understanding the role of lipid metabolite 15d-PGJ2 in myoblast proliferation and differentiation. However, in its current form, the manuscript is incomplete as there are several concerns in the statistical analysis, lack of clarity on the mechanistic details, and concerns about the use of an immortalized C2C12 myoblasts cell line to draw major conclusions related to senescence-associated secreted phenotype.

Reviewed by eLife

Uncovering the BIN1-SH3 interactome underpinning centronuclear myopathy

1. Boglarka Zambo
2. Evelina Edelweiss
3. Bastien Morlet
4. Luc Negroni
5. Matyas Pajkos
6. Zsuzsanna Dosztanyi
7. Soren Ostergaard
8. Gilles Trave
9. Jocelyn Laporte
10. Gergo Gogl

• Curated by eLife

  eLife assessment

  This work describes a novel affinity interactomics approach that allows investigators to identify networks of protein-protein interactions in cells. The important findings presented here describe the application of this technique to the SH3 domain of the membrane remodeling Bridging Integrator 1 (BIN1), the truncation of which leads to centronuclear myopathy. The authors present solid evidence that BIN1 SH3 engages with an unexpectedly high number of cellular proteins, many of which are linked to skeletal muscle disease, and evidence is presented to suggest that BIN1 may play a role in mitosis creating the potential for new avenues in drug development efforts. Some of the findings, however, remain rather preliminary, lack sufficient replicates and may require additional experiments to definitively support the conclusions.

Reviewed by eLife

Impact of protein and small molecule interactions on kinase conformations

1. Valentina Kugler
2. Selina Schwaighofer
3. Andreas Feichtner
4. Florian Enzler
5. Jakob Fleischmann
6. Sophie Strich
7. Sarah Schwarz
8. Rebecca Wilson
9. Philipp Tschaikner
10. Jakob Troppmair
11. Veronika Sexl
12. Pascal Meier
13. Teresa Kaserer
14. Eduard Stefan

• Curated by eLife

  eLife assessment

  This article reports an important bioluminescence-based reporter system to evaluate kinase conformations. This assay is applied to four different kinases that have unique, very special regulatory features, thereby indicating that the assay can be used to provide convincing evidence on the conformational state of a large number of kinases. This paper will be of interest to researchers working on kinases and their conformational states.

Reviewed by eLife

Toxin-based screening of C-terminal tags in Escherichia coli reveals the exceptional potency of ssrA-like degrons

1. Patrick C. Beardslee
2. Karl R. Schmitz

• Curated by eLife

  eLife assessment

  This important study employs an innovative genetic selection-based approach to identify short peptide sequences that target bacterial proteins for degradation. Using random mutagenesis they identified 5 amino acid long "degrons" that target the toxin VapC for degradation permitting survival. They provide compelling data that degrons ending in Ala-Ala are selectively recognized by the ClpXP protease and identify the sequence FKLVA as a particularly significant target. As a whole, there is enthusiasm about the author's findings, although there are also some improvements that could be made to increase the clarity and impact, mostly in the form of revisions to the text.

Reviewed by eLife