Evaluated articles

The effect of linker conformation on performance and stability of a two-domain lytic polysaccharide monooxygenase

1. Zarah Forsberg
2. Anton A. Stepnov
3. Giulio Tesei
4. Yong Wang
5. Edith Buchinger
6. Sandra K. Kristiansen
7. Finn L. Aachmann
8. Lise Arleth
9. Vincent G.H. Eijsink
10. Kresten Lindorff-Larsen
11. Gaston Courtade

Reviewed by Arcadia Science

Improving Split Reporters of Protein–Protein Interactions through Orthology-Based Protein Engineering

1. Louise-Marie Rakotoarison
2. Alison G. Tebo
3. Dorothea Böken
4. Stephanie Board
5. Lina El Hajji
6. Arnaud Gautier

Reviewed by Arcadia Science

Large-scale analyses reveal the contribution of adaptive evolution in pathogenic and non-pathogenic fungal species

1. Danilo Pereira
2. Melvin D Bolton
3. Timothy L Friesen
4. Wolfgang Stephan
5. Julien Y Dutheil
6. Eva H Stukenbrock

Reviewed by Arcadia Science

High-accuracy mapping of human and viral direct physical protein-protein interactions using the novel computational system AlphaFold-pairs

1. Christian Poitras
2. Felix Lamontagne
3. Nathalie Grandvaux
4. Hao Song
5. Maxime Pinard
6. Benoit Coulombe

Reviewed by Arcadia Science

Computational exploration of the global microbiome for antibiotic discovery

1. Célio Dias Santos-Júnior
2. Marcelo Der Torossian Torres
3. Yiqian Duan
4. Álvaro Rodríguez del Río
5. Thomas S.B. Schmidt
6. Hui Chong
7. Anthony Fullam
8. Kuhn Michael
9. Chengkai Zhu
10. Amy Houseman
11. Jelena Somborski
12. Anna Vines
13. Xing-Ming Zhao
14. Peer Bork
15. Jaime Huerta-Cepas
16. Cesar de la Fuente-Nunez
17. Luis Pedro Coelho

Reviewed by Arcadia Science

Harnessing Nature’s nanoSecrets: biocompatibility, biodistribution and bioactivity of extracellular vesicles derived from microalgae

1. Giorgia Adamo
2. Pamela Santonicola
3. Sabrina Picciotto
4. Paola Gargano
5. Aldo Nicosia
6. Valeria Longo
7. Noemi Aloi
8. Daniele P. Romancino
9. Angela Paterna
10. Estella Rao
11. Samuele Raccosta
12. Rosina Noto
13. Monica Salamone
14. Irene Deidda
15. Salvatore Costa
16. Caterina Di Sano
17. Giuseppina Zampi
18. Svenja Morsbach
19. Katharina Landfester
20. Paolo Colombo
21. Mingxing Wei
22. Paolo Bergese
23. Nicolas Touzet
24. Mauro Manno
25. Elia Di Schiavi
26. Antonella Bongiovanni

Reviewed by Arcadia Science

Neuron cilia constrain glial regulators to microdomains around distal neurons

1. Sneha Ray
2. Pralaksha Gurung
3. R. Sean Manning
4. Alexandra Kravchuk
5. Aakanksha Singhvi

Reviewed by Arcadia Science

Neuronal IL-17 controls Caenorhabditis elegans developmental diapause through CEP-1/p53

1. Abhishiktha Godthi
2. Sehee Min
3. Srijit Das
4. Johnny Cruz-Corchado
5. Andrew Deonarine
6. Kara Misel-Wuchter
7. Priya D. Issuree
8. Veena Prahlad

Reviewed by Arcadia Science

Metabolic diversity in commensal protists regulates intestinal immunity and trans-kingdom competition

1. Elias R. Gerrick
2. Soumaya Zlitni
3. Patrick T. West
4. Matthew M. Carter
5. Claire M. Mechler
6. Matthew R. Olm
7. Elisa B. Caffrey
8. Jessica A. Li
9. Steven K. Higginbottom
10. Christopher J. Severyn
11. Frauke Kracke
12. Alfred M. Spormann
13. Justin L. Sonnenburg
14. Ami S. Bhatt
15. Michael R. Howitt

Reviewed by Arcadia Science

AlphaFold2 predicts interactions amidst confounding structural compatibility

1. Juliette Martin

Reviewed by Arcadia Science

Widespread regulation of the maternal transcriptome by Nanos in Drosophila

1. Mohammad Marhabaie
2. Tammy H. Wharton
3. Sung Yun Kim
4. Robin P. Wharton

Reviewed by Arcadia Science

High-throughput tracking enables systematic phenotyping and drug repurposing in C. elegans disease models

1. Thomas J O'Brien
2. Ida L Barlow
3. Luigi Feriani
4. André EX Brown

• Curated by eLife

  eLife Assessment

  This important study provides proof of principle that C. elegans models can be used to accelerate the discovery of candidate treatments for human Mendelian diseases by detailed high-throughput phenotyping of strains harboring mutations in orthologs of human disease genes. The data are compelling and support an approach that enables the potential rapid repurposing of FDA-approved drugs to treat rare diseases for which there are currently no effective treatments. The work will be of interest to all geneticists.

Reviewed by eLife, Arcadia Science

Improved green and red GRAB sensors for monitoring dopaminergic activity in vivo

1. Yizhou Zhuo
2. Bin Luo
3. Xinyang Yi
4. Hui Dong
5. Xiaolei Miao
6. Jinxia Wan
7. John T. Williams
8. Malcolm G. Campbell
9. Ruyi Cai
10. Tongrui Qian
11. Fengling Li
12. Sophia J. Weber
13. Lei Wang
14. Bozhi Li
15. Yu Wei
16. Guochuan Li
17. Huan Wang
18. Yu Zheng
19. Yulin Zhao
20. Marina E. Wolf
21. Yingjie Zhu
22. Mitsuko Watabe-Uchida
23. Yulong Li

Reviewed by Arcadia Science

Comparison of de novo and reference genome-based transcriptome assembly pipelines for differential expression analysis of RNA sequencing data

1. Rebekah A. Oomen
2. Halvor Knutsen
3. Esben M. Olsen
4. Sissel Jentoft
5. Nils Chr. Stenseth
6. Jeffrey A. Hutchings

Reviewed by Arcadia Science

Targeted decontamination of sequencing data with CLEAN

1. Marie Lataretu
2. Sebastian Krautwurst
3. Matthew R. Huska
4. Mike Marquet
5. Adrian Viehweger
6. Sascha D. Braun
7. Christian Brandt
8. Martin Hölzer

Reviewed by Arcadia Science

Epithelial Ca ²⁺ waves triggered by enteric neurons heal the gut

1. Afroditi Petsakou
2. Yifang Liu
3. Ying Liu
4. Aram Comjean
5. Yanhui Hu
6. Norbert Perrimon

Reviewed by Arcadia Science

Centripetal integration of past events in hippocampal astrocytes regulated by locus coeruleus

1. Peter Rupprecht
2. Sian N. Duss
3. Denise Becker
4. Christopher M. Lewis
5. Johannes Bohacek
6. Fritjof Helmchen

Reviewed by Arcadia Science

Binding and sequestration of poison frog alkaloids by a plasma globulin

1. Aurora Alvarez-Buylla
2. Marie-Therese Fischer
3. Maria Dolores Moya Garzon
4. Alexandra E Rangel
5. Elicio E Tapia
6. Julia T Tanzo
7. H Tom Soh
8. Luis A Coloma
9. Jonathan Z Long
10. Lauren A O'Connell

• Curated by eLife

  eLife assessment

  Poison frogs sequester alkaloids to make themselves toxic or unpalatable to predators, but how this sequestration occurs is not well understood. This valuable study identifies an alkaloid-binding protein in the plasma of poison frogs, which may help explain how these animals are able to sequester a diversity of alkaloids with different target sites. The supporting evidence is solid and the study adds to our understanding of how toxic animals resist the effects of their own defenses.

Reviewed by eLife, Arcadia Science

Non-invasive Analysis of Peptidoglycan from Living Animals

1. Karl L. Ocius
2. Sree H. Kolli
3. Saadman S. Ahmad
4. Jules M. Dressler
5. Mahendra D. Chordia
6. Brandon L. Jutras
7. Melanie R. Rutkowski
8. Marcos M. Pires

Reviewed by Arcadia Science

Characterization of a selective, iron-chelating antifungal compound that disrupts fungal metabolism and synergizes with fluconazole

1. Jeanne Corrales
2. Lucia Ramos-Alonso
3. Javier González-Sabín
4. Nicolás Ríos-Lombardía
5. Nuria Trevijano-Contador
6. Henriette Engen Berg
7. Frøydis Sved Skottvoll
8. Francisco Moris
9. Oscar Zaragoza
10. Pierre Chymkowitch
11. Ignacio Garcia
12. Jorrit M. Enserink

Reviewed by Arcadia Science